Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells by Ruley H Earl, Gebre-Amlak Kassatihun D, Jo Daewoong, Lin Qing

“…The most active Cre proteins tested contained either an N-terminal 6xHis tag and a nuclear localization sequence from SV40 large T antigen (HNC) or the HIV Tat transduction sequence and a C-terminal 6xHis tag (TCH₆). …”
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Immunogenicity Analysis of PCV3 Recombinant Capsid Protein Virus-like Particles and Their Application in Antibodies Detection by Xuyang Cao, Min Huang, Ying Wang, Yanzhi Chen, Hanwen Yang, Fusheng Quan

“…In this study, the recombinant Cap protein was successfully expressed in <i>Escherichia coli</i> by deleting the nuclear localization sequence (NLS). The VLPs were observed by transmission electron microscopy. …”
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Development of a split fluorescent protein-based RNA live-cell imaging system to visualize mRNA distribution in plants by Nien-Chen Huang, Kai-Ren Luo, Tien-Shin Yu

“…To minimize the background fluorescence in the cytosol, the nuclear localization sequence has been used to sequester the RBP not bound to mRNA in the nucleus. …”
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The Cif proteins from Wolbachia prophage WO modify sperm genome integrity to establish cytoplasmic incompatibility by Rupinder Kaur, Brittany A. Leigh, Isabella T. Ritchie, Seth R. Bordenstein

“…Finally, CI and rescue are contingent upon a newly annotated CifA bipartite nuclear localization sequence. Together, our results strongly support the Host modification model of CI in which Cifs initially modify the paternal and maternal gametes to bestow CI-defining embryonic lethality and rescue. …”
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Peripheral non-viral MIDGE vector-driven delivery of β-endorphin in inflammatory pain by Busch Melanie, Labuz Dominika, Brack Alexander, Rittner Heike L, Mousa Shaaban A, Sitte Nicolle, Binder Waltraud, Schroff Matthias, Oswald Detlef, Machelska Halina, Wittig Burghardt, Schäfer Michael, Stein Christoph

“…Here, we investigated the effects of a nuclear localization sequence (NLS)-coupled MIDGE encoding the β-endorphin precursor proopiomelanocortin (POMC) on complete Freund's adjuvant-induced inflammatory pain in rats.…”
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Progress and Challenges: Development and Implementation of CRISPR/Cas9 Technology in Filamentous Fungi by Qiang Wang, Jeffrey J. Coleman

“…Potential challenges that are confronted when developing a CRISPR/Cas9 system for filamentous fungi are also discussed such as the nuclear localization sequence for the CAS9 gene, potential off-target effects, and highly efficient transformation methods. …”
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High-Resolution Crystal Structure of Muscle Phosphoglycerate Mutase Provides Insight into Its Nuclear Import and Role by Janusz Wiśniewski, Jakub Barciszewski, Jakub Turlik, Karolina Baran, Przemysław Duda, Mariusz Jaskolski, Dariusz Rakus

“…In this study, we present the first crystal structure of PGAM2, identify the residues involved in the nuclear localization of the protein and propose that PGAM contains a “quaternary nuclear localization sequence (NLS)”, i.e., one that consists of residues from different protein chains. …”
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Exploiting the endogenous yeast nuclear proteome to identify short linear motifs in vivo by Tanner M. Tessier, Cason R. King, Joe S. Mymryk

“…This approach capitalizes on the observation that many nuclear proteins lack an identifiable nuclear localization sequence (NLS) and are co-transported into the nucleus via interaction with proteins containing a true NLS.…”
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High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment by Klaus Braun, Volker Ehemann, Manfred Wiessler, Ruediger Pipkorn, Bernd Didinger, Gabriele Mueller, Waldemar Waldeck

“…The modular-composed carrier consists of a transmembrane transporter (CPP), connected to a nuclear localization sequence (NLS) cleavably-bound, which in turn was coupled with TMZ. …”
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Characterization of the Nucleocytoplasmic Transport Mechanisms of Epstein-Barr Virus BFLF2 by Meili Li, Tao Chen, Xingmei Zou, Zuo Xu, Yuanfang Wang, Ping Wang, Xiaowen Ou, Yiwen Li, Daixiong Chen, Tao Peng, Yao Wang, Mingsheng Cai

“…Methods: Interspecies heterokaryon assays were utilized to detect the nucleocytoplasmic shuttling of BFLF2, and mutation analysis, plasmid transfection and fluorescence microscopy assays were performed to identify the functional nuclear localization sequence (NLS) and nuclear export sequence (NES) of BFLF2 in live cells. …”
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Engineered Post-Translational Logic (PTL) by Sutton, Samantha, Neves, Sara, Leung, Lauren, Endy, Drew

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Histone Deacetylase 5 Limits Cocaine Reward through cAMP-Induced Nuclear Import by Taniguchi, Makoto, Carreira, Maria B., Smith, Laura N., Zirlin, Benjamin C., Neve, Rachael L., Cowan, Christopher W.

“…We show that cAMP-stimulated nuclear import of HDAC5 requires a signaling mechanism that involves transient, protein phosphatase 2A (PP2A)-dependent dephosphorylation of a Cdk5 site (S279) found within the HDAC5 nuclear localization sequence. Dephosphorylation of HDAC5 increases its nuclear accumulation, by accelerating its nuclear import rate and reducing its nuclear export rate. …”
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Role of the ATPase/helicase maleless (MLE) in the assembly, targeting, spreading and function of the male-specific lethal (MSL) complex of <it>Drosophila</it> by Morra Rosa, Yokoyama Ruth, Ling Huiping, Lucchesi John C

“…Deletion of the glycine-rich C-terminal region, which contains a nuclear localization sequence, caused a substantial level of retention of the other MSL proteins in the cytoplasm. …”
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MHO1, an evolutionarily conserved gene, is synthetic lethal with PLC1; Mho1p has a role in invasive growth. by Ivan D Schlatter, Maria Meira, Vanessa Ueberschlag, Dominic Hoepfner, Rao Movva, Nancy E Hynes

“…None of the Memo homologues have a characteristic nuclear localization sequence, however, a conserved nuclear export sequence is found in all. …”
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Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i> by Hua Jiang, Shengyu Gu, Kai Li, Junyi Gai

“…Among the 18 functional ones, GmTGA8 and GmTGA19, with similar motif distribution, nuclear localization sequence and interaction proteins, showed a rapid response to SMV infection and performed better than the others in inhibiting SMV multiplication. …”
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Alternative mRNA Splicing Controls the Functions of the Histone H3K27 Demethylase UTX/KDM6A by Omid Fotouhi, Sheikh Nizamuddin, Stephanie Falk, Oliver Schilling, Ruth Knüchel-Clarke, Martin L. Biniossek, H. T. Marc Timmers

“…Up to half of all stable mRNAs (8–48% in bladder tissues and 18–58% in cell lines) are represented by the UTX canonical isoform lacking exon 14 encoding a nuclear localization sequence, and hence exon 14-containing UTX isoforms exclusively localize to the nucleus, unlike the cytonuclear localization of the canonical isoform. …”
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Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of <i>γ-Thionin</i> Expression by Ekaterina V. Sheshukova, Natalia M. Ershova, Fedor A. Lipskerov, Tatiana V. Komarova

“…Here, we performed synthesis of an artificial nuclear protein—red fluorescent protein (mRFP) fused with a nuclear localization sequence (NLS)—in plant cells as a mimetic of a bacterial effector. …”
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Nuclear Isoforms of Neurofibromin Are Required for Proper Spindle Organization and Chromosome Segregation by Charoula Peta, Emmanouella Tsirimonaki, Dimitris Samouil, Kyriaki Georgiadou, Dimitra Mangoura

“…Cell type- and development-regulated splicings may lead to the inclusion or exclusion of <i>NF1</i>exon51, which bears a nuclear localization sequence (NLS) for nuclear import at G2; yet the functions of the produced NLS and ΔNLS neurofibromin isoforms have not been previously addressed. …”
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Targeted therapies in liver fibrosis: combining the best parts of platelet derived growth factor BB and interferon gamma by Fransien van Dijk, Fransien van Dijk, Peter eOlinga, Klaas ePoelstra, Leonie eBeljaars

“…For that, we altered the cell binding properties of IFNγ, by delivery of the IFNγ-nuclear localization sequence to the highly expressed PDGFβ-receptor using a PDGFβ-receptor recognizing peptide, thereby creating a construct referred to as Fibroferon (i.e. fibroblast-targeted interferon γ). …”
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The subcellular localization of IGFBP5 affects its cell growth and migration functions in breast cancer by Sahin Aysegul, Hu Limei, Akkiprik Mustafa, Hao Xishan, Zhang Wei

“…The mutation occurs within the nuclear localization sequence (NLS) of the protein and is generated by site-directed mutagenesis using the wild-type IGFBP5 expression construct as a template. …”
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