Cytosolic and Nucleosolic Calcium Signaling in Response to Osmotic and Salt Stresses Are Independent of Each Other in Roots of Arabidopsis Seedlings by Feifei Huang, Jin Luo, Tingting Ning, Wenhan Cao, Xi Jin, Heping Zhao, Yingdian Wang, Shengcheng Han

“…Here, we generated transgenic plants containing a fusion protein, comprising rat parvalbumin (PV) with either a nuclear export sequence (PV-NES) or a nuclear localization sequence (NLS-PV), to selectively buffer the cytosolic or nucleosolic calcium. …”
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A bipartite NLS motif mediates the nuclear import of Drosophila moesin by Zoltán Kovács, Zoltán Kovács, Csaba Bajusz, Anikó Szabó, Péter Borkúti, Balázs Vedelek, Réka Benke, Zoltán Lipinszki, Ildikó Kristó, Péter Vilmos

“…We show that the nuclear import of moesin is an NLS-mediated, active process. The nuclear localization sequence of the moesin protein is an evolutionarily highly conserved, conventional bipartite motif located on the surface of the FERM domain. …”
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Cysteine (C)-x-C receptor 4 undergoes transportin 1-dependent nuclear localization and remains functional at the nucleus of metastatic prostate cancer cells. by Ayesha S Don-Salu-Hewage, Siu Yuen Chan, Kathleen M McAndrews, Mahandranauth A Chetram, Michelle R Dawson, Danaya A Bethea, Cimona V Hinton

“…We reveal a putative nuclear localization sequence (NLS), 'RPRK', within CXCR4 that contributed to nuclear localization. …”
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CDPK1 from ginger promotes salinity and drought stress tolerance without yield penalty by improving growth and photosynthesis in Nicotiana tabacum. by Padmanabhan Jayanthi Vivek, Narendra Tuteja, Eppurathu Vasudevan Soniya

“…An important aspect seen during the analysis of the deduced protein is a rare coupling between the presence of a nuclear localization sequence in the junction domain and consensus sequence in the EF-hand loops of calmodulin-like domain. …”
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Generation of a transducible version of a bioactive recombinant human TBX5 transcription factor from E. Coli by Krishna Kumar Haridhasapavalan, Nayan Jyoti Das, Rajkumar P. Thummer

“…First, the protein-coding, codon-optimized TBX5 nucleotide sequence was fused in-frame with three tags (octahistidine, transactivator of transcription, and nuclear localization sequence) at either end of the sequence for affinity purification, cellular uptake, and nuclear delivery. …”
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Nuclear Import of a Secreted “Candidatus Liberibacter asiaticus” Protein is Temperature Dependent and Contributes to Pathogenicity in Nicotiana benthamiana by Xuelu Liu, Xuelu Liu, Yanyan Fan, Yanyan Fan, Chao Zhang, Meixue Dai, Xuefeng Wang, Weimin Li

“…However, NLS-m460, which contains the SV40 nuclear localization sequence (NLS) at the N-terminus to promote nuclear import of m460, caused chlorosis and necrosis in the local leaves and severe necrosis in the systemic leaves. …”
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High‐throughput, microscope‐based sorting to dissect cellular heterogeneity by Nicholas Hasle, Anthony Cooke, Sanjay Srivatsan, Heather Huang, Jason J Stephany, Zachary Krieger, Dana Jackson, Weiliang Tang, Sriram Pendyala, Raymond J Monnat Jr., Cole Trapnell, Emily M Hatch, Douglas M Fowler

“…First, we use Visual Cell Sorting to assess hundreds of nuclear localization sequence variants in a pooled format, identifying variants that improve nuclear localization and enabling annotation of nuclear localization sequences in thousands of human proteins. …”
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Complex Formation with Monomeric α-Tubulin and Importin 13 Fosters c-Jun Protein Stability and Is Required for c-Jun’s Nuclear Translocation and Activity by Melanie Kappelmann-Fenzl, Silke Kuphal, Rosemarie Krupar, Dirk Schadendorf, Viktor Umansky, Lily Vardimon, Claus Hellerbrand, Anja-Katrin Bosserhoff

“…In addition, this complex formation is necessary for c-Jun’s nuclear localization sequence binding to importin 13, and consequent nuclear import and activity of c-Jun. …”
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Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation by Kiyomitsu, Tomomi, Cheeseman, Iain M

“…RanGTP acts in part through the nuclear localization sequence of NuMA to locally alter the ability of NuMA-LGN to associate with the cell cortex in the vicinity of chromosomes. …”
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Molecular radiotherapy using cleavable radioimmunoconjugates that target eGFR and γH2AX by Cornelissen, B, Waller, A, Able, S, Vallis, K, Vallis, K

“…The EGFR ligand EGF was conjugated to a fluorophore- or 111In-labeled anti-γH2AX antibody, linked via a nuclear localization sequence (NLS) to ensure nuclear translocation. …”

Molecular radiotherapy using cleavable radioimmunoconjugates that target EGFR and γH2AX. by Cornelissen, B, Waller, A, Able, S, Vallis, K

“…The EGFR ligand EGF was conjugated to a fluorophore- or (111)In-labeled anti-γH2AX antibody, linked via a nuclear localization sequence (NLS) to ensure nuclear translocation. …”

Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) by Omidvar, Vahid, Abdullah, Siti Nor Akmar, Ho, Chai Ling, Mahmood, Maziah

“…The EgEREBP-encoded protein has a conserved AP2/EREBP DNA-binding domain (DNA-BD), a potential nuclear localization sequence, and a putative activation domain. …”
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Exploring novel and potent cell penetrating peptides in the proteome of SARS-COV-2 using bioinformatics approaches. by Kimia Kardani, Azam Bolhassani

“…Furthermore, the novel and potent CPPs derived from SARS-CoV-2 may be used alone or conjugated to some sequences such as nuclear localization sequence (NLS) for vaccine and drug delivery.…”
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Functional analysis of the <it>theobroma cacao NPR1 </it>gene in <it>arabidopsis</it> by Verica Joseph, Liu Yi, Maximova Siela N, Shi Zi, Guiltinan Mark J

“…The predicted TcNPR1 protein shares 55% identity and 78% similarity to <it>Arabidopsis </it>NPR1, and contains each of the highly conserved functional domains indicative of this class of transcription factors (BTB/POZ and ankyrin repeat protein-protein interaction domains and a nuclear localization sequence (NLS)). To functionally define the <it>TcNPR1 </it>gene, we transferred <it>TcNPR1 </it>into an <it>Arabidopsis npr1 </it>mutant that is highly susceptible to infection by the plant pathogen <it>Pseudomonas syringae </it>pv. tomato DC3000. …”
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The sequential phosphorylation of PHF10 subunit of the PBAF chromatin-remodeling complex determines different properties of the PHF10 isoforms by Andrey A. Sheynov, Victor V. Tatarskiy, Eugene V. Tatarskiy, Elena N. Nabirochkina, Sofia G. Georgieva, Nataliya V. Soshnikova

“…These two subclusters surround a sequence, which is predicted to be a nuclear localization sequence (NLS3). The NLS3 does not affect localization of PHF10 isoforms. …”
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Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells by Kathryn E Kistler, Tatjana Trcek, Thomas R Hurd, Ruoyu Chen, Feng-Xia Liang, Joseph Sall, Masato Kato, Ruth Lehmann

“…Deletion of Oskar’s nuclear localization sequence specifically ablates nuclear granules in cell systems. …”
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Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a by Smith George W, Tejomurtula Jyothsna, Tripurani Swamy K, Lingenfelter Brandon M, Yao Jianbo

“…The protein contains the conserved bipartite nuclear localization sequence and shows 53% and 62% identity with mouse and human NPM2, respectively. …”
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Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae. by Mitchell A Lesko, Dakshayini G Chandrashekarappa, Eric M Jordahl, Katherine G Oppenheimer, Ray W Bowman, Chaowei Shang, Jacob D Durrant, Martin C Schmidt, Allyson F O'Donnell

“…To achieve its role in glucose repression, Hxk2 reportedly binds the Mig1 transcriptional repressor, is dephosphorylated at serine 15 and requires an N-terminal nuclear localization sequence (NLS). We used high-resolution, quantitative, fluorescent microscopy of live cells to determine the conditions, residues, and regulatory proteins required for Hxk2 nuclear localization. …”
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TRIM22 promotes the proliferation of glioblastoma cells by activating MAPK signaling and accelerating the degradation of Raf-1 by Xiaowei Fei, Ya-nan Dou, Kai Sun, Jialiang Wei, Qingdong Guo, Li Wang, Xiuquan Wu, Weihao Lv, Xiaofan Jiang, Zhou Fei

“…In addition, deletion of the RING domain and the nuclear localization sequence of TRIM22 significantly inhibited TRIM22-induced proliferation of GBM cells in vivo and in vitro. …”
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TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy by Rachel Atkinson, Jacqueline Leung, James Bender, Matthew Kirkcaldie, James Vickers, Anna King

“…Mouse (C57BL/6J) retinal ganglion cells (RGCs) were transduced with GFP-tagged human wild-type TDP-43 (hTDP-WT-GFP) and human TDP-43 with a mutation in the nuclear localization sequence (hTDP-ΔNLS-GFP), to cause TDP-43 mislocalization, with ∼60% transduction efficiency achieved. …”
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