Absence of PTHrP nuclear localization and carboxyl terminus sequences leads to abnormal brain development and function. by Zhen Gu, Yahong Liu, Yongjie Zhang, Shulei Jin, Qi Chen, David Goltzman, Andrew Karaplis, Dengshun Miao

“…We assessed whether the nuclear localization sequences (NLS) and C terminus of parathyroid hormone-related protein (PTHrP) play critical roles in brain development and function. …”
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Calcium-Activated Big-Conductance (BK) Potassium Channels Traffic through Nuclear Envelopes into Kinocilia in Ray Electrosensory Cells by Abby L. Chen, Ting-Hsuan Wu, Lingfang Shi, William T. Clusin, Peter N. Kao

“…BK and CaV1.3 are co-expressed in electrosensory cell plasma membranes, nuclear envelopes and kinocilia. Nuclear localization sequences (NLS) were predicted in BK and CaV1.3 by bioinformatic sequence analyses. …”
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Characterization of the nuclear import mechanism of the CCAAT-regulatory subunit Php4. by Md Gulam Musawwir Khan, Jean-François Jacques, Jude Beaudoin, Simon Labbé

“…Php4 nuclear import relies on two functionally independent nuclear localization sequences (NLSs) that are located between amino acid residues 171 to 174 (KRIR) and 234 to 240 (KSVKRVR). …”
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Mammary mechanisms for lactoferrin: interactions with IGFBP-3. by Baumrucker C.R.

“…Both IGFBP-3 and Lf have nuclear localization sequences that are reported to he key components of nuclear localization of proteins. …”
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HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity by Wenwei Luo, Jingyan Li, Ziqing Li, Tong Lin, Lili Zhang, Wanqi Yang, Yanqi Mai, Ruiming Liu, Meiting Chen, Chunmei Dai, Hanwei Yang, Jing Lu, Hong Li, Guimei Guan, Min Huang, Peiqing Liu, Zhuoming Li

“…In addition, infection of recombinant adenovirus encoding ΔHO-1 with three nuclear localization sequences (NLS), alleviated endothelial senescence induced by knockdown of endogenous HO-1 by CRISPR/Cas9. …”
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Fragmentation of Nimotuzumab for Preparation of 125I-F(ab’)2-Nimotuzumab as a Precursor for Preparing 125I-F(ab’)2-Nimotuzumab-NLS Radiopharmaceutical for Cancer Therapy by R.D. Haryuni, A. Bahtiar, S. Soenarjo, Y. Harahap, A. Mutalib, M. Ramli, S. Hermanto, C.N. Ardiyatno, V.Y. Susilo, D. Haffid

“…Therefore in this study nimotuzumab was fragmented to form bivalent antibody [F(ab’)2] and then labeled with 125I to form 125I-F(ab’)2-nimotuzumab which can be used further as a precursor for preparing 125I-F(ab’)2-nimotuzumab-NLS (NLS = nuclear localizing sequences ) radiopharmaceutical for radioimmunotherapy. …”
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Development of an indirect immunofluorescence assay for PCV3 antibody detection based on capsid protein by Lun Yao, Chang Li, Junwei Wang, Yufang Cheng, Ahmed H. Ghonaim, Qi Sun, Xuexiang Yu, Weijie Niu, Shengxian Fan, Qigai He

“…Expression of the nuclear localization sequences was further analyzed by IFA. Strong and specific fluorescence signals were observed in the nucleus of rvBac-Pc-transfected cells and in the cytoplasm of rvBac-Sc-transfected cells. …”
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A versatile endosome acidity-induced sheddable gene delivery system: increased tumor targeting and enhanced transfection efficiency by Zhao M, Li J, Ji H, Chen D, Hu H

“…The novel carrier exhibited a few picturesque characteristics, including (i) neutral surface charge to restrain nonspecific interactions; (ii) folate receptors (FR)-mediated endocytosis to augment cellular uptake; (iii) dual proton sponge effect to realize endosome escape, and (iv) nuclear localization sequences (NLS) to enhance the transfection of pDNA.Results: FPCPNDs could compress and protect pDNA from degradation. …”
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Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment by Patrick F Forde, Lindsay J Hall, Mira Sadadcharam, Marcle de Kruijf, Gerald C O'Sullivan, Declan M Soden

“…To overcome these expression difficulties, we developed a proof-of-concept vector enhanced expression vector (EEV), which incorporates elements from viral systems including nuclear localization sequences and a viral replicase from the Semliki Forest virus. …”
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<i>Proteus mirabilis</i> Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity by Matheus V. C. Grahl, Augusto F. Uberti, Valquiria Broll, Paula Bacaicoa-Caruso, Evelin F. Meirelles, Celia R. Carlini

“…Bioinformatic analysis revealed two bipartite nuclear localization sequences in PMU. We have shown that PMU, besides urinary stone formation, can potentially contribute in other ways to pathogenesis. …”
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Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae by Megan E. Goeckel, Erianna M. Basgall, Isabel C. Lewis, Samantha C. Goetting, Yao Yan, Megan Halloran, Gregory C. Finnigan

“…We examine non-native nuclear localization sequences (both NLS and NES) on Cas9 fusion proteins in vivo through fluorescence microscopy and genomic editing. …”
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Nucleocytoplasmic shuttling activity of ataxin-3. by Sandra Macedo-Ribeiro, Luísa Cortes, Patrícia Maciel, Ana Luísa Carvalho

“…In order to characterize the nuclear shuttling activity of Atx3, we performed yeast nuclear import assays and found that Atx3 is actively imported into the nucleus, by means of a classical nuclear localizing sequence formed by a cluster of lysine and arginine residues. …”
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Localization and translocation of mature miRNAs by A.E. Abaturov, V.L. Babуch

“…These proteins recognize nuclear localization sequences (NLS) in the amino acid sequences of proteins and actively transport these proteins through the pores of the cell’s nuclear membrane. …”
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Adenovirus hexon protein enhances nuclear delivery and increases transgene expression of polyethylenimine/plasmid DNA vectors. by Carlisle, R, Bettinger, T, Ogris, M, Hale, S, Mautner, V, Seymour, L

“…The ability of hexon to promote nuclear delivery of PEI/DNA nanoparticles was compared with that of classical nuclear localization sequences (NLS) by measuring transgene expression following intracytoplasmic microinjection of hexon-PEI/DNA complexes and NLS-albumin-PEI/DNA complexes in rat-1 fibroblasts. …”

Adenovirus hexon protein enhances nuclear delivery and increases transgene expression of polyethylenimine/plasmid DNA vectors by Carlisle, RC, Bettinger, T, Ogris, M, Hale, S, Mautner, V, Seymour, LW

“…The ability of hexon to promote nuclear delivery of PEI/DNA nanoparticles was compared with that of classical nuclear localization sequences (NLS) by measuring transgene expression following intracytoplasmic microinjection of hexon–PEI/DNA complexes and NLS–albumin–PEI/DNA complexes in rat-1 fibroblasts. …”

Application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of Influenza A virus strain by Atreya Chintamani D, Ketha Krishna

“…<p>Abstract</p> <p>Background</p> <p>Two nuclear localization sequences (NLS) in influenza A virus nucleoprotein (NP) have been demonstrated to be critical for nuclear import of NP and viral ribonucleoprotein complexes. …”
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Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression by Gutierrez-Hartmann Arthur, Koto Karen, McConnell Brice V

“…To investigate the respective roles of cytoplamsic and nuclear LIMK1 in breast cancer progression, we targeted GFP-LIMK1 to cytoplasmic and nuclear subcellular compartments by fusing nuclear export signals (NESs) or nuclear localization sequences (NLS), respectively, to the amino-terminus of GFP-LIMK1. …”
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123I-labeled HIV-1 tat peptide radioimmunoconjugates are imported into the nucleus of human breast cancer cells and functionally interact in vitro and in vivo with the cyclin-depen... by Hu, M, Chen, P, Wang, J, Scollard, D, Vallis, K, Reilly, R

“…METHODS: Peptides [GRKKRRQRRRPPQGYGC] harboring the nuclear-localizing sequence from HIV tat domain were conjugated to anti-p21(WAF-1/Cip-1) antibodies. …”

111In-labeled immunoconjugates (ICs) bispecific for the epidermal growth factor receptor (EGFR) and cyclin-dependent kinase inhibitor, p27Kip1. by Cornelissen, B, Kersemans, V, McLarty, K, Tran, L, Reilly, R

“…Our aim was to construct immunoconjugates (ICs) bispecific for epidermal growth factor receptors (EGFRs) and the intranuclear cyclin-dependent kinase inhibitor (CDKI) p27(Kip1) modified with nuclear-localizing sequences (NLSs) to facilitate their nuclear uptake following EGFR-mediated internalization. …”

Trastuzumab-resistant breast cancer cells remain sensitive to the auger electron-emitting radiotherapeutic agent 111In-NLS-trastuzumab and are radiosensitized by methotrexate. by Costantini, D, Bateman, K, McLarty, K, Vallis, K, Reilly, R

“…UNLABELLED: Our goals in this study were to determine whether (111)In-trastuzumab coupled to peptides harboring nuclear localizing sequences (NLSs) could kill trastuzumab-resistant breast cancer cell lines through the emission of Auger electrons and whether the combination of radiosensitization with methotrexate (MTX) would augment the cytotoxicity of this radiopharmaceutical. …”