Elevated temperature during slicing enhances acute slice preparation quality by Shiwei eHuang, Marylka Yoe Uusisaari, Marylka Yoe Uusisaari

“…The enhanced quality of brain slices from old animals facilitates experimentation on age-related disorders as well as optogenetic studies requiring long transfection periods.…”
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Peripersonal encoding of forelimb proprioception in the mouse somatosensory cortex by Ignacio Alonso, Irina Scheer, Mélanie Palacio-Manzano, Noémie Frézel-Jacob, Antoine Philippides, Mario Prsa

“…We delineated the rodent proprioceptive cortex with wide-field calcium imaging and optogenetic silencing experiments during behavior. Our results reveal that proprioception is represented in both sensory and motor cortical areas. …”
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Learning prediction error neurons in a canonical interneuron circuit by Loreen Hertäg, Henning Sprekeler

“…By balancing excitation and inhibition in multiple pathways, experience-dependent inhibitory plasticity can generate different variants of prediction-error circuits, which can be distinguished by simulated optogenetic experiments. The experience-dependence of the model circuit is consistent with that of negative prediction-error circuits in layer 2/3 of mouse primary visual cortex. …”
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Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males by Hayato M. Yamanouchi, Ryoya Tanaka, Azusa Kamikouchi

“…An RNA-seq database search and subsequent mutant analysis revealed the importance of piezo for maintaining male copulation posture. piezo-GAL4-positive signals were found in the sensory neurons of male genitalia bristles, and optogenetic inhibition of piezo-expressing neurons in the posterior side of the male body during copulation destabilized posture and terminated copulation. …”
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Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies by Jonathan CY Tang, Eugene Drokhlyansky, Behzad Etemad, Stephanie Rudolph, Binggege Guo, Sui Wang, Emily G Ellis, Jonathan Z Li, Constance L Cepko

“…Fusion of destabilized Nbs to various proteins enabled applications in living cells, such as optogenetic control of neural activity in specific cell types in the mouse brain, and detection of HIV-infected human cells by flow cytometry. …”
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Cortical Sensory Responses Are Enhanced by the Higher-Order Thalamus by Rebecca A. Mease, Markus Metz, Alexander Groh

“…While primary thalamocortical nuclei transmit sensory signals from the periphery, the function of higher-order thalamocortical projections remains enigmatic, in particular their role in sensory processing in the cortex. Here, by optogenetically controlling the thalamocortical pathway from the higher-order posteromedial thalamic nucleus (POm) during whisker stimulation, we demonstrate the integration of the two thalamocortical streams by single pyramidal neurons in layer 5 (L5) of the mouse barrel cortex under anesthesia. …”
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Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed by Daniel C. Williams, Rachid El Bejjani, Paula Mugno Ramirez, Sean Coakley, Shin Ae Kim, Hyewon Lee, Quan Wen, Aravi Samuel, Hang Lu, Massimo A. Hilliard, Marc Hammarlund

“…Here, we show that the optogenetic tool KillerRed selectively, rapidly, and permanently inactivates different classes of neurons in C. elegans in response to a single light stimulus, through the generation of reactive oxygen species (ROS). …”
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Dopamine release at the time of a predicted aversive outcome causally controls the trajectory and expression of conditioned behavior by Munir Gunes Kutlu, Jennifer Tat, Brooke A. Christensen, Jennifer E. Zachry, Erin S. Calipari

“…This dopamine response is largest when the omission is unexpected and decreases over learning, and artificially increasing this signal disrupts discrimination learning. Conversely, optogenetic inhibition of dopamine responses to the footshock itself impairs learning. …”
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Value representations in the rodent orbitofrontal cortex drive learning, not choice by Kevin J Miller, Matthew M Botvinick, Carlos D Brody

“…Electrophysiological recordings and optogenetic perturbations indicate that these representations do not directly drive choice. …”
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A Dual Operator View of Habitual Behavior Reflecting Cortical and Striatal Dynamics by Smith, Kyle S., Graybiel, Ann M., Smith, Kyle S., Graybiel, Ann M.

“…A similar pattern developed in the upper layers of the infralimbic cortex, but it formed only late during overtraining and closely tracked habit states. Selective optogenetic disruption of infralimbic activity during overtraining prevented habit formation. …”
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Oxytocin Mediates Entrainment of Sensory Stimuli to Social Cues of Opposing Valence by Choe, Han Kyoung, Reed, Michael Douglas, Benavidez, Nora, Montgomery, Daniel P, Soares, Natalie, Yim, Yeong Shin, Choi, Bohyun Gloria, Choe, Han Kyoung, Reed, Michael Douglas

“…We used odor-driven behavioral paradigms to ask if oxytocin, a neuropeptide implicated in various social behaviors, plays a crucial role in the formation of learned associations between odor and socially significant cues. Through genetic, optogenetic, and pharmacological manipulations, we show that oxytocin receptor signaling is crucial for entrainment of odor to social cues but is dispensable for entrainment to nonsocial cues. …”
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Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis by Kotýnková, Kristýna, Su, Kuan-Chung, West, Stephen C., Petronczki, Mark

“…Using chemical genetic and optogenetic tools, we demonstrate that the association of ECT2 with the plasma membrane during anaphase is required and sufficient for cytokinesis. …”
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Amygdala-ventral striatum circuit activation decreases long-term fear by dos Santos Correia, Susana Maria, McGrath, Anna, Lee, Allison M., Graybiel, Ann M, Goosens, Ki Ann

“…Enhanced recruitment of this circuit during extinction learning, either by pairing reward with fear extinction training or by optogenetic stimulation of this circuit during fear extinction, reduces the return of fear that normally follows extinction training. …”
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Adaptive and multifunctional hydrogel hybrid probes for long-term sensing and modulation of neural activity by Park, Seongjun, Yuk, Hyunwoo, Zhao, Ruike, Yim, Yeong Shin, Woldeghebriel, Eyob W, Kang, Jeewoo, Canales, Andres, Fink, Yoel, Choi, Gloria B, Zhao, Xuanhe, Anikeeva, Polina

“…The hydrogel hybrid devices permit electrophysiological, optogenetic, and behavioral studies of neural circuits with minimal foreign body responses and tracking of stable isolated single neuron potentials in freely moving mice over 6 months following implantation.…”
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Polymer Fiber Probes Enable Optical Control of Spinal Cord and Muscle Function In Vivo by Canales, Andres, Caggiano, Vittorio, Bizzi, Emilio, Anikeeva, Polina Olegovna, Froriep, Ulrich Paul, Koppes, Ryan, Selvidge, Jennifer G., Lu, Chi, Ph. D. Massachusetts Institute of Technology

“…Using our fiber probes, we demonstrate simultaneous recording and optogenetic stimulation of neural activity in the spinal cord of transgenic mice expressing the light sensitive protein channelrhodopsin 2 (ChR2). …”
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Upward synaptic scaling is dependent on neurotransmission rather than spiking by Fong, Ming-fai, Newman, Jonathan P., Potter, Steve M., Wenner, Peter

“…Here we combined chronic multielectrode recording, closed-loop optogenetic stimulation, and pharmacology to show that reduced glutamatergic transmission directly triggers cell-wide synaptic upscaling. …”
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Precision of Discrete and Rhythmic Forelimb Movements Requires a Distinct Neuronal Subpopulation in the Interposed Anterior Nucleus by Low, Aloysius Y. T., Thanawalla, Ayesha Rustom, Yip, Alaric K. K., Kim, Jinsook, Wong, Kelly L. L., Tantra, Martesa, Augustine, George James, Chen, Albert I-Ming

“…We show that these neurons represent a subset of glutamatergic neurons in the IntA and constitute a specific element of an internal feedback circuit within the cerebellar cortex and cerebello-thalamo-cortical pathway associated with limb control. Ablation and optogenetic stimulation of these neurons disrupt efficacy of skilled reach and locomotor movement and reveal that they control positioning and timing of the forelimb and hindlimb. …”
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Optical control of excitation waves in cardiac tissue by Burton, R, Klimas, A, Ambrosi, C, Tomek, J, Corbett, A, Entcheva, E, Bub, G

“…Here, we combine dye-free optical imaging with optogenetic actuation to achieve dynamic control of cardiac excitation waves. …”

Long-term restoration of visual function in end-stage retinal degeneration using subretinal human melanopsin gene therapy by De Silva, SR, Barnard, AR, Hughes, S, Tam, SKE, Martin, C, Singh, MS, Barnea-Cramer, AO, McClements, ME, During, MJ, Peirson, SN, Hankins, MW, MacLaren, RE

“…Optogenetic strategies to restore vision in patients who are blind from end-stage retinal degenerations aim to render remaining retinal cells light sensitive once photoreceptors are lost. …”

Current approaches to characterize micro- and macroscale circuit mechanisms of Parkinson’s disease in rodent models by Peng, Y, Schöneberg, N, Esposito, MS, Geiger, JRP, Sharott, A, Tovote, P

“…In this review, we will introduce classic and novel methods ranging from single-cell electrophysiological recordings to state-of-the-art calcium imaging and cell-type specific optogenetic or chemogenetic stimulation. We will focus on their application in rodent models of Parkinson’s disease while also presenting their use in the context of motor control and basal ganglia function. …”