Seroepidemiology of pertussis in the east of China: Estimates of incidence of infection in adolescents and adults pre- and post-COVID-19 by Qiang Chen, Wen Wang, Xiuyun Shi, Yan Xu, Yanhong Zhu, Yun Wu, Zhiguo Wang, Hui Sun, Xiang Sun

“…The objective was to compare the estimated incidence rate of pertussis in populations pre- and post-COVID-19 pandemic by analyzing the anti-pertussis toxin (anti-PT) IgG and anti-filamentous hemagglutininant (anti-FHA) IgG antibodies in healthy Chinese population from 2018 to 2021.MethodsAll serum samples (N = 1,000) were collected from healthy population (aged ≥ 15 years) who attended an annual monitoring project of antibody levels in Jiangsu province in 2018–2021 were measured by ELISA.ResultsThe positive rates of anti-PT IgG and anti-FHA IgG antibodies were 11.4% (114/1,000) and 20.2% (202/1,000) (≥40 IU/ml), the GMC were 17.25 (95% CI: 15.49–19.03) IU/mL and 24.94 (95% CI: 22.73–27.16) IU/mL in the study population, respectively. …”
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Montelukast for postinfectious cough in adults: A double-blind randomised placebo-controlled trial by Wang, K, Taylor, K, Jin, J, Perera, R, Farmer, A, Mant, D, Harnden, A, Birring, S, Fry, N, Harrison, T, Hay, A, Moore, M, Little, P

“…Patients were tested for pertussis (oral fluid anti-pertussis toxin IgG) and randomly assigned (1:1) to montelukast 10 mg daily or image-matched placebo for 2 weeks. …”

PEPITEM Treatment Ameliorates EAE in Mice by Reducing CNS Inflammation, Leukocyte Infiltration, Demyelination, and Proinflammatory Cytokine Production by Mohammed Alassiri, Fahd Al Sufiani, Mohammed Aljohi, Asma Alanazi, Aiman Saud Alhazmi, Bahauddeen M. Alrfaei, Hasan Alnakhli, Yasser A. Alshawakir, Saleh M. Alharby, Abdullah Y. Almubarak, Mohammed Alasseiri, Nora Alorf, Mashan L. Abdullah

“…The disease was induced using MOG35-55 and complete Freund’s adjuvants augmented with pertussis toxin. The EAE score was recorded daily until the end of the experiment (21 days). …”
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The Eukaryotic Host Factor 14-3-3 Inactivates Adenylate Cyclase Toxins of <named-content content-type="genus-species">Bordetella bronchiseptica</named-content> and <named-content c... by Aya Fukui-Miyazaki, Hirono Toshima, Yukihiro Hiramatsu, Keisuke Okada, Keiji Nakamura, Keisuke Ishigaki, Naoaki Shinzawa, Hiroyuki Abe, Yasuhiko Horiguchi

“…The toxins of B. bronchiseptica and presumably B. parapertussis were inactivated by the host factor 14-3-3 after phosphorylation in target cells, whereas the B. pertussis toxin was not inactivated because of the lack of the phosphorylation site. …”
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Montelukast for postinfectious cough in adults: a double-blind randomised placebo-controlled trial. by Wang, K, Birring, S, Taylor, K, Fry, N, Hay, A, Moore, M, Jin, J, Perera, R, Farmer, A, Little, P, Harrison, T, Mant, D, Harnden, A

“…Patients were tested for pertussis (oral fluid anti-pertussis toxin IgG) and randomly assigned (1:1) to montelukast 10 mg daily or image-matched placebo for 2 weeks. …”

Montelukast for postinfectious cough in adults: a double-blind randomised placebo-controlled trial by Wang, K, Birring, S, Taylor, K, Fry, N, Hay, A, Moore, M, Jin, J, Perera, R, Farmer, A, Little, P, Harrison, T, Mant, D, Harnden, A

“…Patients were tested for pertussis (oral fluid anti-pertussis toxin IgG) and randomly assigned (1:1) to montelukast 10 mg daily or image-matched placebo for 2 weeks. …”

Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3 by Samira A. Hamed, Armaan Mohan, Saranya Navaneetha Krishnan, Arthur Wang, Marija Drikic, Nicole L. Prince, Ian A. Lewis, Jane Shearer, Åsa V. Keita, Johan D. Söderholm, Timothy E. Shutt, Derek M. McKay

“…The use of the G-protein inhibitor, pertussis toxin, implicated GPCR signaling as critical to the effect of butyrate, and the free fatty acid receptor three (FFAR3, GPR41) agonist, AR420626, reproduced butyrate’s effect in terms of ameliorating the loss of barrier function and reducing the mitochondrial fragmentation observed in E. coli-LF82 infected T84-cells and organoids. …”
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Regulation of FGF2-induced proliferation by inhibitory GPCR in normal pituitary cells by Liliana del V. Sosa, Liliana del V. Sosa, Florencia Picech, Florencia Picech, Pablo Perez, Pablo Perez, Silvina Gutierrez, Silvina Gutierrez, Rodrigo Bainy Leal, Ana De Paul, Ana De Paul, Alicia Torres, Alicia Torres, Juan Pablo Petiti, Juan Pablo Petiti

“…The aim of this study was to evaluate whether GPCR-Gαi modulates basic fibroblast growth factor (FGF2)-induced proliferative activity in normal pituitary cell populations.MethodsAnterior pituitary primary cell cultures from Wistar female rats were treated with FGF2 (10ng/mL) or somatostatin analog (SSTa, 100nM) alone or co-incubated with or without the inhibitors of GPCR-Gαi, pertussis toxin (PTX, 500nM), MEK inhibitor (U0126, 100µM) or PI3K inhibitor (LY 294002, 10 μM).ResultsFGF2 increased and SSTa decreased the lactotroph and somatotroph BrdU uptak2e (p&lt;0.05) whereas the FGF2-induced S-phase entry was prevented by SSTa co-incubation in both cell types, with these effects being reverted by PTX, U0126 or LY294002 pre-incubation. …”
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Dextran-enhanced CEST MRI reveals the size effect of BBB dysfunction associated with neuroinflammation by Wenshen Wang, Jiadi Xu, Aline Thomas, Guanshu Liu

“…EAE MS mouse model: C57Bl/6 mice (F/6-10w), were injected s.c. with myelin peptide (MOG35-55, 200 μL, 0.5 mg/mL) emulsified in incomplete Freund's adjuvant supplemented with M. tuberculosis H37Ra (5 mg/mL) and i.p. with 300 ng of pertussis toxin on days 0 and 2. Mice were observed daily for signs of paralysis using a 0-5 rating system. …”
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Optimising DTwP-containing vaccine infant immunisation schedules (OptImms) — a protocol for two parallel, open-label, randomised controlled trials by Sarah Kelly, Xinxue Liu, Katherine Theiss-Nyland, Merryn Voysey, Sarah Murphy, Grace Li, Mary Nyantaro, Meeru Gurung, Sudha Basnet, Bhishma Pokhrel, Sanjeev M. Bijukchhe, Agnes Eordogh, Ben Gombe, Ayoub Kakande, Simon Kerridge, Geofrey Kimbugwe, Sylvia Kusemererwa, Lawrence Lubyayi, Henry Luzze, Olga Mazur, Yama F. Mujadidi, Shamim Nabukenya, Walter-Rodney Nagumo, Tryphena Nareeba, Rabiullah Noristani, Peter O’Reilly, Andrew Roberts, Ganesh Shah, Sonu Shrestha, Laxman P. Shrestha, Surya B. Thapa, Freddie M. Kibengo, Arun K. Sharma, Alison Elliott, Shrijana Shrestha, Andrew J. Pollard

“…The primary outcome is anti-pertussis toxin IgG antibodies measured at the time of the booster dose. …”
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A semi high-throughput whole blood-based flow cytometry assay to detect and monitor Bordetella pertussis-specific Th1, Th2 and Th17 responses by Véronique Corbière, Eleonora E. Lambert, Marine Rodesch, Jacqueline A. M. van Gaans-van den Brink, Alicja Misiak, Elles Simonetti, Elles Simonetti, Anne Van Praet, Audrey Godefroid, Dimitri A. Diavatopoulos, Dimitri A. Diavatopoulos, Cécile A. C. M. van Els, Cécile A. C. M. van Els, Françoise Mascart, PERISCOPE WP5 Task 7 working group

“…Blood samples collected from recently acellular (aP) vaccine boosted subjects with a whole-cell- or aP-primed background was incubated for 24 hrs with Pertussis toxin, Filamentous hemagglutinin or a Bp lysate (400µl per stimulation). …”
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Antioxidant effect of grape seed extract corrects experimental autoimmune encephalomyelitis behavioral dysfunctions, demyelination, and glial activation by Maha Mabrouk, Maha Mabrouk, Maha Mabrouk, Maha Mabrouk, Mohamed El Ayed, Mohamed El Ayed, Amélie Démosthènes, Amélie Démosthènes, Youssef Aissouni, Youssef Aissouni, Ezzedine Aouani, Ezzedine Aouani, Laurence Daulhac-Terrail, Laurence Daulhac-Terrail, Meherzia Mokni, Meherzia Mokni, Mélina Bégou, Mélina Bégou

“…We presently evaluated the curative effect of grape seed extract (GSE) in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.Experimental approachSix-week-old C57Bl/6J females were subjected to the EAE paradigm (using myelin oligodendrocyte glycoprotein peptide fragment (35-55), complete Freund’s adjuvant, and pertussis toxin) and then chronically treated with GSE from day 10 to day 30 post-induction. …”
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Persistence of antibody response following a booster dose of Hib-MenC-TT glycoconjugate vaccine to five years: a follow-up study. by Khatami, A, Snape, MD, Wysocki, J, John, T, Westcar, S, Mesaros, N, Peddiraju, K, Boutriau, D, Yu, L, Pollard, A

“…Antibody concentrations against filamentous hemagglutinin, pertactin and pertussis toxin were also measured at this visit. RESULTS: Two hundred sixty-eight participants aged 58-64 months were enrolled. …”

Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China by Pan Fu, Jinlan Zhou, Jianhua Meng, Zhiwei Liu, Yaxier Nijiati, Leiyan He, Chunling Li, Saige Chen, Aimin Wang, Gangfeng Yan, Guoping Lu, Lijun Zhou, Xiaowen Zhai, Chuanqing Wang

“…There were six multilocus variable-number tandem-repeat analysis types (MTs), including MT28 (62.0%), MT195 (20%), MT27 (10.0%), MT104 (4.7%), MT55 (2.7%), and MT32 (0.7%). BP strains with pertussis toxin (ptx)P3/(pertactin) prn2/ptxC2/ptxA1/(fimbrial proteins) fim2-1/fim3-1, including MT27, MT28, and MT32, accounted for 72.7%, among which MT27 and MT32 were macrolide-sensitive BP, whereas most (94.6∼100%) of MT28 were MRBP. …”
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Stable Levels of Antibodies Against Unrelated Toxoid Vaccines After COVID-19: COVID-19 Infection Does Not Affect Toxoid Vaccine Antibody Levels by Suvi T. Jokiranta, Simo Miettinen, Sami Salonen, Lauri Kareinen, Ruut Uusitalo, Essi M. Korhonen, Jenni Virtanen, Ilkka Kivistö, Kirsi Aaltonen, Dina A. Mosselhy, Tinja Lääveri, Anu Kantele, T. Petteri Arstila, Hanna Jarva, Olli Vapalahti, Santtu Heinonen, Eliisa Kekäläinen

“…We measured IgG antibodies to diphtheria toxin, tetanus toxoid, and pertussis toxin, along with total IgG, SARS-CoV-2 spike protein IgG, and neutralizing antibodies. …”
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Low Glucose plus β-Hydroxybutyrate Induces an Enhanced Inflammatory Response in Yak Alveolar Macrophages via Activating the GPR109A/NF-κB Signaling Pathway by Jiancheng Qi, Qiyuan Yang, Qing Xia, Fangyuan Huang, Hongrui Guo, Hengmin Cui, Yue Xie, Zhihua Ren, Liping Gou, Dongjie Cai, Maqsood Ahmed Kumbhar, Jing Fang, Zhicai Zuo

“…A pretreatment of either pertussis toxin (PTX, inhibitor of GPR109A) or pyrrolidinedithiocarbamic (PDTC, inhibitor of NF-κB p65) was effective in preventing the elevated secretion of pro-inflammatory cytokines induced by low glucose plus BHB (<i>p</i> < 0.05). …”
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Safety and immunogenicity of a single dose of Tdap compared to Td in pregnant women in Mali and 3 its effect on infant immune responses: a single-centre, randomised, double-blind,... by Fadima Cheick Haidara, Milagritos D. Tapia, Fatoumata Diallo, Susana Portillo, Margaret Williams, Awa Traoré, Elizabeth Rotrosen, Elizabeth Hensel, Mat Makowski, Semhal Selamawi, Jonathan A. Powell, Karen L. Kotloff, Marcela F. Pasetti, Samba O. Sow, Kathleen M. Neuzil

“…We aimed to evaluate the safety of a single dose of tetanus, diphtheria and acellular pertussis vaccine (Tdap) vaccine compared to tetanus and diphtheria vaccine (Td) vaccine in pregnant women in Bamako, Mali and to assess the pertussis toxin (PT) antibody response at birth. Methods: In this phase 2, single-centre, randomised, double-blind, active-controlled study, from 23 January 2019 to 10 July 2019, healthy 18–39 year old women in the second trimester of a singleton pregnancy were randomised 2:1 to receive Tdap or Td. …”
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Memory B Cell Activation Induced by Pertussis Booster Vaccination in Four Age Groups of Three Countries by Pauline Versteegen, Alex-Mikael Barkoff, Marta Valente Pinto, Jan van de Kasteele, Aapo Knuutila, Sagida Bibi, Lia de Rond, Johanna Teräsjärvi, Katherine Sanders, Mary-lène de Zeeuw-Brouwer, Raakel Luoto, Hinke ten Hulscher, Elizabeth A. Clutterbuck, Elisabeth A. M. Sanders, Elisabeth A. M. Sanders, Jussi Mertsola, Guy A. M. Berbers, Qiushui He, Dominic F. Kelly, Dominic F. Kelly, Anne-Marie Buisman, PERISCOPE Consortium

“…Memory B cells at baseline, day 28, and 1 year post-vaccination were measured by a pertussis toxin (Ptx), filamentous haemagglutinin (FHA), and pertactin (Prn) specific ELISpot assay. …”
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