Non-homologous End Joining-Mediated Insertional Mutagenesis Reveals a Novel Target for Enhancing Fatty Alcohols Production in Yarrowia lipolytica by Mengxu Li, Mengxu Li, Jinlai Zhang, Jinlai Zhang, Qiuyan Bai, Qiuyan Bai, Lixia Fang, Lixia Fang, Hao Song, Hao Song, Yingxiu Cao, Yingxiu Cao

“…Whole-genome sequencing of the high-yield strain demonstrated that a new target YALI0_A00913g (“A1 gene”) was disrupted by NHEJ-mediated integration of partial carrier DNA, and reverse engineering of the A1 gene disruption (YlΔA1-FAR) recovered the fatty alcohol overproduction phenotype. Transcriptome analysis of YlΔA1-FAR strain revealed A1 disruption led to strengthened protein synthesis process that was confirmed by sfGFP gene expression, which may account for enhanced cell viability and improved biosynthesis of fatty alcohols. …”
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Canthaxanthin Attenuates the Vascular Aging or Endothelial Cell Senescence by Inhibiting Inflammation and Oxidative Stress in Mice by Zhefeng Wang, Lilin Li, Souqi Liao, Ren Huang, Yibo Jiang, Jia Fei, Lijun Cai, Keda Zhang

“…Background: Vascular endothelial dysfunction is an early phenotype of aging-related vascular dysfunction. Delaying vascular aging and preventing cardiovascular disease are major public health problems that urgently need to be solved. …”
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First evidence for STING SNP R293Q being protective regarding obesity-associated cardiovascular disease in age-advanced subjects – a cohort study by Lutz Hamann, Malgorzata Szwed, Malgorzata Mossakowska, Jerzy Chudek, Monika Puzianowska-Kuznicka

“…Since current smoking triggers DNA damage that, similar to obesity, may result in the release of DNA into the cytoplasm, we hypothesized that the cGAS/STING pathway can modify the phenotype of aging also in obese subjects. Therefore, the objective of our study was to investigate whether STING R293Q is associated with aging-related diseases in obese individuals. …”
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Older Patients Are Immunocompromised by Cytokine Depletion and Loss of Innate Immune Function After HIP Fracture Surgery by Alasdair G. Sutherland MD, FRCSEd (T&O), Alistair Cook PhD, Clare Miller MBChB, Linda Duncan BSc, Raif Yuecel PhD, Steven D. Heys PhD, FRCS, James D. Hutchison PhD, FRCSEd, Janet Liversidge PhD

“…Methods: In a cohort observational study, 16 patients undergoing surgery for hip fractures had immune function evaluation prior to surgery, and then at 3 and 7 days postoperatively, using flow cytometry for phenotype and for monocyte and granulocyte phagocytic function and respiratory burst. …”
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Cytokine profile and nitric oxide levels in peritoneal macrophages of BALB/c mice exposed to the fucose-mannose ligand of Leishmania infantum combined with glycyrrhizin by Hasan Namdar Ahmadabad, Reza Shafiei, Gholam Reza Hatam, Reza Zolfaghari Emameh, Ashok Aspatwar

“…Conclusions Therefore, we concluded that GL can improve the immunostimulatory effect of FML on macrophages and leads to their polarization towards an M1-like phenotype.…”
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Evaluation of genetic diversity of 22 rice (Oryza sativa) landraces native to northern Iran in terms of some germination components and growth indices by SAMANEH ELYASI, VAHID MOLLASADEGHI, SHAPOUR ABDOLLAHI

“…In order to study genetic diversity of 22 rice (Oryza sativa L.) landraces native to northern part of the country in terms of germination components and of some growth indices, an experiment was conducted at laboratory of agriculture faculty of Islamic Azad University, Ardabil branch, as randomized complete blocks design with three replications, in year 2011. Estimation of phenotypic and genotypic coefficients for various traits revealed that genotypes being studied were more genetically diverse in terms of FGP, GI, GRI, Rs, VI, seedling length, radicle length and total fresh weight of sample than in terms of other traits. …”
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Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis by Ludmila V. Sakhno, Ekaterina Ya. Shevela, Marina A. Tikhonova, Sergey D. Nikonov, Alexandr A. Ostanin, Elena R. Chernykh

“…The phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-reactive versus PPD-anergic patients). …”
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Molecular Mechanisms behind the Physiological Resistance to Intense Transient Warming in an Iconic Marine Plant by Lazaro Marín-Guirao, Laura Entrambasaguas, Emanuela Dattolo, Juan M. Ruiz, Gabriele Procaccini

“…Shallow plants, living in a more unstable thermal environment, optimized phenotype variation in response to warming. These plants showed a pre-adaptation of genes in anticipation of stress. …”
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Quantitative Real Time PCR study on the effects of hilA gene deletion on the expression of pathogenicity genes in Salmonella enterica ssp. by Maryam Safarpour-Dehkordi, Abbas Doosti, Mohammad-Saeid Jami, Abolfazl Gholipour

“…The hilA gene encodes a transcriptional activator that regulates expression of the majority of the genes responsible for the Salmonella invasive phenotype. In the present study, PCR was performed using our designed primers for amplification of upstream and downstream regions of Salmonella typhimurium hilA gene. …”
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High-fat diet-induced acceleration of osteoarthritis is associated with a distinct and sustained plasma metabolite signature by Poulami Datta, Yue Zhang, Alexa Parousis, Anirudh Sharma, Evgeny Rossomacha, Helal Endisha, Brian Wu, Izabela Kacprzak, Nizar N. Mahomed, Rajiv Gandhi, Jason S. Rockel, Mohit Kapoor

“…This study suggests that in addition to increased weight, identified metabolites and local leptin-signaling may also contribute in part, towards the accelerated OA-phenotype observed in HFD mice.…”
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IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo by Miriam Weiss, Katrina Blazek, Adam J. Byrne, Dany P. Perocheau, Irina A. Udalova

“…Thus, we establish the species-invariant role of IRF5 in controlling the inflammatory macrophage phenotype both in vitro and in in vivo.…”
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Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons by C. Tubert, E. Zampese, T. Pancani, T. Tkatch, D.J. Surmeier

“…Thus, PPN CNs have a distinctive physiological phenotype that shares some, but not all, of the features of other neurons that are selectively vulnerable in PD.…”
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Tensile Stress‐Activated and Exosome‐Transferred YAP/TAZ‐Notch Circuit Specifies Type H Endothelial Cell for Segmental Bone Regeneration by Feng Wang, Shanyu Li, Lingchi Kong, Kai Feng, Rongtai Zuo, Hanzhe Zhang, Yifan Yu, Kunqi Zhang, Yuting Cao, Yimin Chai, Qinglin Kang, Jia Xu

“…Mechanistically, cell‐matrix interactions‐mediated activation of yes‐associated protein (YAP)/transcriptional co‐activator with PDZ‐binding motif (TAZ) transcriptionally upregulates the expression of Notch1 and Delta‐like ligand 4, which act as direct positive regulators of THECs phenotype, in bone marrow endothelial cells (BMECs) upon TS stimulation. …”
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Interleukin-11/IL-11 Receptor Promotes Glioblastoma Cell Proliferation, Epithelial–Mesenchymal Transition, and Invasion by Sarah F. Stuart, Peter Curpen, Adele J. Gomes, Michelle C. Lan, Shuai Nie, Nicholas A. Williamson, George Kannourakis, Andrew P. Morokoff, Adrian A. Achuthan, Rodney B. Luwor

“…Furthermore, IL-11Rα-overexpressing cells displayed a more mesenchymal-like phenotype compared to parental cells and expressed greater levels of the mesenchymal marker Vimentin. …”
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Lipids regulate the hydrolysis of membrane bound glucosylceramide by lysosomal β-glucocerebrosidase by Misbaudeen Abdul-Hammed, Bernadette Breiden, Günter Schwarzmann, Konrad Sandhoff

“…Our data suggest that these strong modifiers of GlcCer hydrolysis affect the genotype-phenotype correlation in several cases of Gaucher patients independent of the types.…”
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Stable Regulation of Cell Cycle Events in Mycobacteria: Insights From Inherently Heterogeneous Bacterial Populations by Michelle M. Logsdon, Michelle M. Logsdon, Bree B. Aldridge, Bree B. Aldridge, Bree B. Aldridge

“…This innate asymmetry creates unequal birth sizes and growth rates for daughter cells with each division, generating a phenotypically heterogeneous population. Although the asymmetric growth patterns of mycobacteria lead to a larger variation in birth size than typically seen in model bacterial populations, the cell size distribution is stable over time. …”
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Genetic Regulatory Perturbation of Gene Expression Impacted by Genomic Introgression in Fiber Development of Allotetraploid Cotton by Xinyuan Chen, Xiubao Hu, Guo Li, Corrinne E. Grover, Jiaqi You, Ruipeng Wang, Zhenping Liu, Zhengyang Qi, Xuanxuan Luo, Yabin Peng, Mengmeng Zhu, Yuqi Zhang, Sifan Lu, Yuan‐ming Zhang, Zhongxu Lin, Jonathan F. Wendel, Xianlong Zhang, Maojun Wang

“…Abstract Interspecific genomic introgression is an important evolutionary process with respect to the generation of novel phenotypic diversity and adaptation. A key question is how gene flow perturbs gene expression networks and regulatory interactions. …”
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Entomopathogenic potential of indigenous Simplicillium subtropicum (Hypocreales: Cordycipitaceae) isolates from Tamil Nadu, India, against the cotton aphid, Aphis gossypii Glover (... by Krishnaveni Mariappan, Jeyarajan Nelson Samuel, Nakkeeran Sevugapperumal, Sivakumar Uthandi, Mohankumar Subbarayalu, Premalatha Krishnamoorthi

“…In order to develop biopesticides for the management of aphids in an environmentally safe manner, 6 indigenous entomopathogenic fungi Simplicillium subtropicum were isolated from soils of Tamil Nadu and their pathogenicity was tested against A. gossypii under laboratory conditions after characterization. Results Phenotypic and molecular characterization was performed for all the isolates for species identification. …”
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Effect of depth gradient and temperature on Zostera marina vegetative and reproductive phenology in the first year of the 1997–1998 El Niño by Alf Enrique Meling-López, Silvia Emilia Ibarra-Obando, Horacio de la Cueva, Pedro Ortega-Romero, Adriana Leticia Navarro-Verdugo

“… Does Zostera marina exhibit phenotypic plasticity, maximizing fitness in traits responding to environmental factors, i.e., depth and temperature? …”
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Comparative Genomic Analyses Reveal Core-Genome-Wide Genes Under Positive Selection and Major Regulatory Hubs in Outlier Strains of Pseudomonas aeruginosa by Utkarsh Sood, Utkarsh Sood, Princy Hira, Roshan Kumar, Roshan Kumar, Roshan Kumar, Abhay Bajaj, Abhay Bajaj, Desiraju Lakshmi Narsimha Rao, Rup Lal, Rup Lal, Mallikarjun Shakarad

“…We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. Virulence genotype analysis revealed that strain CR1 lacked hemolytic phospholipase C and D, three genes for LPS biosynthesis and had reduced antibiotic resistance genes when compared with clinical strains. …”
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