Investigating miR-9 as a mediator in laryngeal cancer health disparities by Christina Gobin, Samuel Inkabi, Chayil C. Lattimore, Tongjun Gu, James N. Menefee, Mayrangela Rodriguez, Heather Kates, Christopher Fields, Tengfei Bian, Natalie Silver, Chengguo Xing, Clayton Yates, Clayton Yates, Clayton Yates, Rolf Renne, Mingyi Xie, Kristianna M. Fredenburg

“…In this study, we present our computational analysis of differentially expressed miRNAs in Black compared with White laryngeal cancer and further validate microRNA-9-5p (miR-9-5p) as a potential mediator of cancer phenotype and chemoresistance.MethodsBioinformatic analysis of 111 (92 Whites, 19 Black) laryngeal squamous cell carcinoma (LSCC) specimens from the TCGA revealed miRNAs were significantly differentially expressed in Black compared with White LSCC. …”
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CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME by Igor Vladimirovich Kudryavtsev, Natalia Mihailovna Lazareva, Olga Petrovna Baranova, Alexey Sergeevich Golovkin, Dmitry Valer’evich Isakov, Maria Konstantinovna Serebriakova, Tatyana Pavlovna Ses’, Mikhail Mikhailovich Ilkovich, Areg Artemovich Totolian

“…There are two main phenotypes of pulmonary sarcoidosis (PS): Lofgren's syndrome (LS) is an acute form with favorable outcome, while non-Lofgren's syndrome (nLS) is a chronic type of disease that can lead to pulmonary fibrosis in 20 % of cases. …”
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Mortality risk factors in primary Sjögren syndrome: a real-world, retrospective, cohort studyResearch in context by Pilar Brito-Zerón, Alejandra Flores-Chávez, Ildiko Fanny Horváth, Astrid Rasmussen, Xiaomei Li, Peter Olsson, Arjan Vissink, Roberta Priori, Berkan Armagan, Gabriela Hernandez-Molina, Sonja Praprotnik, Luca Quartuccio, Nevsun Inanç, Burcugül Özkızıltaş, Elena Bartoloni, Agata Sebastian, Vasco C. Romão, Roser Solans, Sandra G. Pasoto, Maureen Rischmueller, Carlos Galisteo, Yasunori Suzuki, Virginia Fernandes Moça Trevisani, Cecilia Fugmann, Andrés González-García, Francesco Carubbi, Ciprian Jurcut, Toshimasa Shimizu, Soledad Retamozo, Fabiola Atzeni, Benedikt Hofauer, Sheila Melchor-Díaz, Tamer Gheita, Miguel López-Dupla, Eva Fonseca-Aizpuru, Roberto Giacomelli, Marcos Vázquez, Sandra Consani, Miriam Akasbi, Hideki Nakamura, Antónia Szántó, A. Darise Farris, Li Wang, Thomas Mandl, Angelica Gattamelata, Levent Kilic, Katja Perdan Pirkmajer, Kerem Abacar, Abdurrahman Tufan, Salvatore de Vita, Hendrika Bootsma, Manuel Ramos-Casals, S. Arends, E. Treppo, S. Longhino, V. Manfrè, M. Rizzo, C. Baldini, S. Bombardieri, M. Bandeira, M. Silvéiro-António, R. Seror, X. Mariette, G. Nordmark, D. Danda, P. Wiland, R. Gerli, S.K. Kwok, S.H. Park, M. Kvarnstrom, M. Wahren-Herlenius, S. Downie-Doyle, D. Sene, D. Isenberg, V. Valim, V. Devauchelle-Pensec, A. Saraux, J. Morel, C. Morcillo, P.E. Díaz Cuiza, B.E. Herrera, L. González-de-Paz, A. Sisó-Almirall

“…The key determinants that defined the disease phenotype at diagnosis (glandular, systemic, and immunological) were analysed as independent variables. …”
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Comparison of Primary Virus Isolation in Pulmonary Alveolar Macrophages and Four Different Continuous Cell Lines for Type 1 and Type 2 Porcine Reproductive and Respiratory Syndrome... by Jiexiong Xie, Nick Vereecke, Sebastiaan Theuns, Dayoung Oh, Nathalie Vanderheijden, Ivan Trus, Jannes Sauer, Philip Vyt, Caroline Bonckaert, Christian Lalonde, Chantale Provost, Carl A. Gagnon, Hans Nauwynck

“…In conclusion, PK15^Sn-CD163 and PK15^S10-CD163 cells are phenotypically closely related to the in vivo target macrophages and are more suitable for virus isolation and titration than MARC-145/MARC-145^Sn cells. …”
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Neuroinflammation, autoinflammation, splenomegaly and anemia caused by bi-allelic mutations in IRAK4 by Samantha Cooray, Fiona Price-Kuehne, Ying Hong, Ebun Omoyinmi, Alice Burleigh, Alice Burleigh, Kimberly C. Gilmour, Bilal Ahmad, Sangdun Choi, Mohammad W. Bahar, Paul Torpiano, Andrey Gagunashvili, Barbara Jensen, Evangelos Bellos, Evangelos Bellos, Vanessa Sancho-Shimizu, Vanessa Sancho-Shimizu, Jethro A. Herberg, Jethro A. Herberg, Kshitij Mankad, Atul Kumar, Marios Kaliakatsos, Austen J. J. Worth, Despina Eleftheriou, Elizabeth Whittaker, Elizabeth Whittaker, Paul A. Brogan

“…We therefore highlight that bi-allelic mutation in IRAK4 may be associated with a severe and complex autoinflammatory and neuroinflammatory phenotype that we have called NASA (neuroinflammation, autoinflammation, splenomegaly and anemia), in addition to immunodeficiency in humans.…”
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Long-Lived Individuals Show a Lower Burden of Variants Predisposing to Age-Related Diseases and a Higher Polygenic Longevity Score by Guillermo G. Torres, Janina Dose, Tim P. Hasenbein, Marianne Nygaard, Ben Krause-Kyora, Jonas Mengel-From, Kaare Christensen, Karen Andersen-Ranberg, Daniel Kolbe, Wolfgang Lieb, Matthias Laudes, Siegfried Görg, Stefan Schreiber, Andre Franke, Amke Caliebe, Gregor Kuhlenbäumer, Almut Nebel

“…Longevity is a complex phenotype influenced by both environmental and genetic factors. …”
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Faecal microbiota transplantation from young rats attenuates age‐related sarcopenia revealed by multiomics analysis by Xiaoxing Mo, Lihui Shen, Ruijie Cheng, Pei Wang, Lin Wen, Yunhong Sun, Qiang Wang, Juan Chen, Shan Lin, Yuxiao Liao, Wei Yang, Hong Yan, Liegang Liu

“…Correlation analysis revealed that yFMT‐induced alterations of gut microbiota and metabolites might be closely related to mitochondria‐related genes and sarcopenia‐related phenotypes. Conclusions yFMT could reshape the dysbiosis of gut microbiota and metabolites, maintain gut barrier integrity, and improve muscle mitochondrial dysfunction, eventually alleviating sarcopenia in aged rats. yFMT might be a new therapeutic strategy for age‐related sarcopenia.…”
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Transferrin receptor 1 ablation in satellite cells impedes skeletal muscle regeneration through activation of ferroptosis by Hongrong Ding, Shujie Chen, Xiaohan Pan, Xiaoshuang Dai, Guihua Pan, Ze Li, Xudong Mai, Ye Tian, Susu Zhang, Bingdong Liu, Guangchao Cao, Zhicheng Yao, Xiangping Yao, Liang Gao, Li Yang, Xiaoyan Chen, Jia Sun, Hong Chen, Mulan Han, Yulong Yin, Guohuan Xu, Huijun Li, Weidong Wu, Zheng Chen, Jingchao Lin, Liping Xiang, Jun Hu, Yan Lu, Xiao Zhu, Liwei Xie

“…Between Tfr1SC/WT and Tfr1SC/KO (n = 6–8 mice per group), cardiotoxin was intramuscularly injected, and transverse abdominal muscle was dissected, weighted, and cryosectioned, followed by immunostaining, haematoxylin and eosin staining, and Masson staining. These phenotypical analyses were followed with functional analysis such as flow cytometry, tread mill, Prussian blue staining, and transmission electron microscopy to identify pathological pathways that contribute to regeneration defects. …”
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The Positive and Negative Syndrome Scale for Schizophrenia Autism Severity Scale (PAUSS) in a sample of early-onset psychosis by J. Suárez Campayo, L. Pina-Camacho, J. Merchán-Naranjo, C. Ordas, V. Cavone, R. Panadero, G. Sugranyes, I. Baeza, J. Castro-Fornieles, E. de la Serna, C. Arango, C. M. Diaz Caneja

“…Objectives The aim of the study was to investigate the prevalence of autistic traits and the diagnostic, developmental, clinical, and functional correlates of this phenotype in a sample of early-onset psychosis (onset before age 18 years; EOP). …”
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A new aggressive xenograft model of human colon cancer using cancer-associated fibroblasts by Ester Fernando-Macías, Maria Teresa Fernández-García, Eva García-Pérez, Belén Porrero Guerrero, Camilo López-Arévalo, Raquel Rodríguez-Uría, Sandra Sanz-Navarro, José Fernando Vázquez-Villa, María Carmen Muñíz-Salgueiro, Laura Suárez-Fernández, José A. Galván, Clara Barneo-Caragol, Marcos García-Ocaña, Juan R. de los Toyos, Luis Barneo-Serra

“…Stromal cell purity was evaluated by morphology and immunostaining with vimentin (VIM) as a fibroblast marker and anti-proColXIα1 as a specific human CAF marker. Phenotypic characterization of cultured stromal cells was performed by co-staining with mesenchymal and epithelial cell markers. …”
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Pyrethroid resistance status and co-occurrence of V1016G, F1534C and S989P mutations in the Aedes aegypti population from two dengue outbreak counties along the China-Myanmar borde... by Li Chen, Kemei Zhou, Jun Shi, Yuting Zheng, Xiaotao Zhao, Qingyun Du, Yingkun Lin, Xaioxiong Yin, Jinyong Jiang, Xinyu Feng

“…A possible association between the observed resistant phenotype and mutations in the voltage-gated sodium channel gene (VGSC) was also studied. …”
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siRNA-Mediated B7H7 Knockdown in Gastric Cancer Lysate-Loaded Dendritic Cells Amplifies Expansion and Cytokine Secretion of Autologous T Cells by Javad Masoumi, Farid Ghorbaninezhad, Hossein Saeedi, Sahar Safaei, Vahid Khaze Shahgoli, Amir Ghaffari Jolfayi, Bahar Naseri, Amir Baghbanzadeh, Elham Baghbani, Ahad Mokhtarzadeh, Mohammad Bakhshivand, Mohammad Reza Javan, Nicola Silvestris, Behzad Baradaran

“…B7H7-siRNA was delivered into mDCs using electroporation, and gene silencing efficiency was assessed. The phenotypic characteristics of iDCs, mDCs, and B7H7-silenced mDCs were evaluated using specific surface markers, an inverted light microscope, and flow cytometry. …”
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Circulating Regulatory T Cell Subsets in Patients with Sarcoidosis by Igor Kudryavtsev, Yulia Zinchenko, Anna Starshinova, Maria Serebriakova, Anna Malkova, Tatiana Akisheva, Dmitriy Kudlay, Anzhela Glushkova, Piotr Yablonskiy, Yehuda Shoenfeld

“…Furthermore, we want to declare that analysis of Treg numbers of phenotypes could fully characterize their functional activity in peripherally inflamed tissues.…”
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Detección de Betalactamasas de espectro extendido en Enterobacteriaceae en un Centro de Salud de Maracaibo, Venezuela by Perozo Mena Armindo, Marín Milagros, Castellano Maribel, Ling Toledo Eliana, Núñez Daniela, Ginestre Messaria, Villasmil Jessica, Bermúdez-González José, Villalobos Rafael, Gómez-Gamboa Liliana

“…The Kirby-Baüer method was perform to preliminary phenotypic detection of ESBL, according to CLSI guidelines. …”
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Le chêne faginé (Quercus faginea, Fagaceae) en Algérie : potentiel germinatif et variabilité morphologique des glands et des semis by Abdeldjalil Aissi, Yassine Beghami, Myriam Heuertz

“…Prior to germination, phenotypic seed traits were measured and a set of physical treatments was performed. …”
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A combination vaccine against SARS-CoV-2 and H1N1 influenza based on receptor binding domain trimerized by six-helix bundle fusion core by Rui Shi, Jiawei Zeng, Ling Xu, Fengze Wang, Xiaomin Duan, Yue Wang, Zheng Wu, Dandan Yu, Qingrui Huang, Yong-Gang Yao, Jinghua Yan

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O-GlcNAcylation regulates extracellular signal-regulated kinase (ERK) activation in Alzheimer’s disease by Sophiya John Ephrame, Gentry K. Cork, Victoria Marshall, Margaret A. Johnston, Jenna Shawa, Ibtihal Alghusen, Amy Qiang, Aspin R. Denson, Marisa S. Carman, Halyna Fedosyuk, Russell H. Swerdlow, Russell H. Swerdlow, Russell H. Swerdlow, Chad Slawson, Chad Slawson

“…Furthermore, 1-month TMG injection was sufficient to increase the amplitude of p-ERK in 5XFAD AD mice brains suggesting AD phenotype contributes to the acceleration of ERK activation mediated by OGA inhibition.ConclusionTogether, these results indicate that disruptions to O-GlcNAc homeostasis amplify ERK signal activation in AD.…”
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Results of molecular genetic screening of mutations in the NLRP3, TNFRSF1A, and MVK genes in patients with autoinflammatory diseases and systemic juvenile arthritis by S. O. Salugina, E. A. Kamenets, E. S. Fedorov, E. Yu. Zakharova, M. I. Kaleda

“…About one-quarter of the patients who have an inflammatory phenotype, including the manifestations of SJA, suffer from monogenic AIDs. …”
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Spatial Metrics of Interaction between CD163-Positive Macrophages and Cancer Cells and Progression-Free Survival in Chemo-Treated Breast Cancer by Brenton A. Maisel, Misung Yi, Amy R. Peck, Yunguang Sun, Jeffrey A. Hooke, Albert J. Kovatich, Craig D. Shriver, Hai Hu, Marja T. Nevalainen, Takemi Tanaka, Nicole Simone, Li Lily Wang, Hallgeir Rui, Inna Chervoneva

“…An objective and automated algorithm was developed to phenotype CD<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mn>163</mn><mo>+</mo></msup></semantics></math></inline-formula> TAMs and calculate their density within the tumor stroma and derive several spatial metrics of interaction with cancer cells. …”
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Activation of eIF4E‐binding‐protein‐1 rescues mTORC1‐induced sarcopenia by expanding lysosomal degradation capacity by Elisa M. Crombie, Seonyoung Kim, Stuart Adamson, Han Dong, Tzu‐Chiao Lu, Yiju Wu, Yajun Wu, Yotam Levy, Nolan Stimple, Wing Moon R. Lam, Hwee Weng D. Hey, Dominic J. Withers, Ao‐Lin Hsu, Boon Huat Bay, Julien Ochala, Shih‐Yin Tsai

“…We discovered a previously unidentified phenotype of lysosomal failure in TSC1mKO mouse muscle, in which the lysosomal defect was also conserved in the naturally aged mouse muscle, whereas 4EBP1 activation enhanced lysosomal protease activities to compensate for impaired autophagy induced by mTORC1 hyperactivity. …”
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