Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China by Biao Tang, Juan Ni, Juan Ni, Jiahui Lin, Yangying Sun, Hui Lin, Yuehong Wu, Hua Yang, Min Yue, Min Yue

“…Here, eight cfr-positive E. coli strains were screened using PCR from an extensive collection of E. coli (n = 2,165) strains isolated from pigs and chickens in 2021 in China, with a prevalence rate of 0.37%. …”
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Single-nucleus and bulk RNA sequencing reveal cellular and transcriptional mechanisms underlying lipid dynamics in high marbled pork by Liyi Wang, Xueyan Zhao, Shiqi Liu, Wenjing You, Yuqin Huang, Yanbing Zhou, Wentao Chen, Shu Zhang, Jiying Wang, Qiankun Zheng, Yizhen Wang, Tizhong Shan

“…However, the cell dynamics and transcriptional programs underlying lipid deposition in highly marbled meat are still unclear. Here, we used Laiwu pigs with high (HLW) or low (LLW) IMF contents to explore the cellular and transcriptional mechanisms underlying lipid deposition in highly-marbled pork by single-nucleus RNA sequencing (snRNA-seq) and bulk RNA sequencing. …”
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Mask R-CNN provides efficient and accurate measurement of chondrocyte viability in the label-free assessment of articular cartilage by Hongming Fan, Pei Xu, Xun Chen, Yang Li, Zhao Zhang, Jennifer Hsu, Michael Le, Emily Ye, Bruce Gao, Harry Demos, Hai Yao, Tong Ye

“…Method: TPAF/SHG images were acquired simultaneously on cartilage samples from rats and pigs using two-photon microscopes and were merged to form RGB color images with red, green, and blue channels assigned to emission bands of oxidized flavoproteins, reduced forms of nicotinamide adenine dinucleotide, and SHG signals, respectively. …”
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Non-ablative doses of focal ionizing radiation alters function of central neural circuits by Hamed Zaer, Wei Fan, Dariusz Orlowski, Andreas N. Glud, Morten B. Jensen, Esben S. Worm, Slávka Lukacova, Trine W. Mikkelsen, Lise M. Fitting, Liisa M. Jacobsen, Thomas Portmann, Jui-Yi Hsieh, Christopher Noel, Georg Weidlich, Woody Chung, Patrick Riley, Cesare Jenkins, John R. Adler, Jr., M. Bret Schneider, Jens Christian H. Sørensen, Albrecht Stroh

“…Six months post-irradiation, the pigs were implanted with a 9 mm-wide, eight-shank multi-electrode probe, which spanned the radiation focus as well as the low-exposure neighboring areas. …”
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Putrescine mitigates intestinal atrophy through suppressing inflammatory response in weanling piglets by Bangmin Liu, Xianren Jiang, Long Cai, Xuemei Zhao, Zhaolai Dai, Guoyao Wu, Xilong Li

“…To evaluate the effects of putrescine on alleviating intestinal atrophy and underlying molecular mechanisms, both in vivo feeding trial and in vitro cell culture were conducted. Weanling pigs were fed a diet supplemented with 0, 0.1%, 0.2% or 0.3% putrescine dihydrochloride, whereas porcine intestinal epithelial cells (IPEC-J2) were challenged with lipopolysaccharide (LPS) in the presence of 200 μmol/L putrescine. …”
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In Vitro Analysis of TGF-β Signaling Modulation of Porcine Alveolar Macrophages in Porcine Circovirus Type 2b Infection by Shunli Yang, Muhammad Umar Zafar Khan, Baohong Liu, Muhammad Humza, Shuanghui Yin, Jianping Cai

“…Our study may provide insights into the underlying antiviral functional changes in the immune system of PCV2-infected pigs.…”
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Real-time feedback on chest compression efficacy by hands-free carotid Doppler in a porcine model by Bjørn Ove Faldaas, Erik Waage Nielsen, Benjamin Stage Storm, Knut Tore Lappegård, Bent Aksel Nilsen, Gabriel Kiss, Eirik Skogvoll, Hans Torp, Charlotte Björk Ingul

“…Methods: In this model of animal cardiac arrest, we induced ventricular fibrillation in five domestic pigs. Manual chest compressions were performed for ten seconds at three different positions on the sternum in random order and repeated six times. …”
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Tantalum-specific contrast-to-noise ratio or conventional detector dose-driven exposure control in angiography: radiation dose and image quality evaluation in a porcine model by Thomas Werncke, Timo Christian Meine, Jan B. Hinrichs, Sabine K. Maschke, Lena Sophie Becker, Inga Brüsch, Regina Rumpel, Frank K. Wacker, Bernhard C. Meyer

“…Methods Nine angiography scenarios were created in seven anaesthetised pigs using Ta-based embolisation material during percutaneous liver and kidney intervention. …”
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The ultrasonically treated nanoliposomes containing PCV2 DNA vaccine expressing gC1qR binding site mutant Cap is efficient in mice by Qian Du, Qian Du, Tengfei Shi, Tengfei Shi, Huaxin Wang, Huaxin Wang, Changlei Zhu, Changlei Zhu, Nan Yang, Nan Yang, Dewen Tong, Dewen Tong, Yong Huang, Yong Huang

“…Nowadays, vaccines are broadly used to prevent porcine circovirus type 2 (PCV2) infection-induced expenditures, but the virus is still spreading among pigs. The current PCV2 vaccines all rely on the immunogenicity of Cap, yet our previous studies found that Cap is also the major component mediating the PCV2 infection-induced immune suppression through its interaction with host gC1qR. …”
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Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2) by Supatra Areekit, Pongbun Tangjitrungrot, Sintawee Khuchareontaworn, Kankanit Rattanathanawan, Pornpun Jaratsing, Montri Yasawong, Gaysorn Chansiri, Nareerat Viseshakul, Kosum Chansiri

“…No cross-reaction to each other and to other pathogenic viruses that can infect pigs were observed according to analytical specificity tests. …”
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DIAGNOSTICS OF THE INTESTINE ISCHEMIC DAMAGES IN SOME ACUTE SURGICAL DISEASES OF ABDOMINAL CAVITY by Vil M. Timerbulatov, Shamil V. Timerbulatov, Ravil B. Sagitov, Artur U. Sultanabaev, Dmitriy I. Asmanov

“…In order to study the role of intra-abdominal hypertension in the developing ischemic infringements of abdominal organs, as well as lactate as a marker of ischemia, the simulation of the abdominal compartment syndrome by a tense pneumoperitoneum under pressure of 20 mm Hg. within two hours was carried out on 5 pigs weighing 60-70 kg and 7 piglets weighing 8-10 kg. …”
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Foot-and-mouth disease in elephants in Kerala state of India during 2013 by MANORANJAN ROUT, NANDAKUMAR S. NAIR, BISWAJIT DAS, SARAVANAN SUBRAMANIAM, JAJATI KESHARI MOHAPATRA, BRAMHADEV PATTNAIK

“…Foot-and-mouth disease (FMD) is a highly contagious acute vesicular disease of the cloven-hoofed animals including cattle, buffalo, sheep, goats, pigs along with more than 70 wildlife species. During the year 2013, FMD outbreaks were recorded in the southern peninsular India comprising the states of Karnataka, Tamil Nadu, Kerala and Andhra Pradesh. …”
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Subnormothermic Ex Vivo Porcine Kidney Perfusion Improves Energy Metabolism: Analysis Using 31P Magnetic Resonance Spectroscopic Imaging by Thomas Agius, MS, Julien Songeon, MS, Antoine Klauser, PhD, Florent Allagnat, PhD, Grégoire Longchamp, MD, Raphael Ruttimann, BS, Arnaud Lyon, MD, Julijana Ivaniesevic, PhD, Raphael Meier, MD, PhD, Sébastien Déglise, MD, James F. Markmann, MD, PhD, Korkut Uygun, PhD, Leo Buhler, MD, Christian Toso, MD, PhD, Jean-Marc Corpataux, MD, Francois Lazeyras, PhD, Alban Longchamp, MD, PhD

“…To mimic donation after circulatory death procurement, kidneys from 45-kg pigs underwent 60 min of warm ischemia. Kidneys were then perfused ex vivo for 4 h with Belzer machine perfusion solution UW at 22 °C or at 4 °C before transplantation. …”
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SVCT2 Overexpression and Ascorbic Acid Uptake Increase Cortical Neuron Differentiation, Which Is Dependent on Vitamin C Recycling between Neurons and Astrocytes by Katterine Salazar, Francisca Espinoza, Gustavo Cerda-Gallardo, Luciano Ferrada, Rocío Magdalena, Eder Ramírez, Viviana Ulloa, Natalia Saldivia, Ninoschka Troncoso, María José Oviedo, María José Barahona, Fernando Martínez, Francisco Nualart

“…Other studies have shown that vitamin C-deprived guinea pigs have reduced spatial memory, suggesting that ascorbic acid (AA) and SVCT2 have important roles in postnatal neuronal differentiation and neurite formation. …”
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Video analysis of ex vivo beating hearts during preservation on the TransMedics® organ care system by Michelle Mendiola Pla, Silvia Berrettoni, Franklin H. Lee, Giacomo Rozzi, Federica Marrano, Ryan T. Gross, Amy Evans, David C. Wendell, Paul Lezberg, Margherita Burattini, Margherita Burattini, Francesco Paolo lo Muzio, Lorenzo Fassina, Carmelo A. Milano, Marie-Louise Bang, Marie-Louise Bang, Dawn E. Bowles, Michele Miragoli, Michele Miragoli

“…We applied a video algorithm for an in vivo assessment of cardiac kinematics, video kinematic evaluation (Vi.Ki.E.), to the donor hearts undergoing ex vivo perfusion on the OCS™ to assess the feasibility of applying this algorithm in this setting.MethodsHealthy donor porcine hearts (n = 6) were procured from Yucatan pigs and underwent 2 h of normothermic ex vivo perfusion on the OCS™ device. …”
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Evaluation of a Porcine Endogenous Reference Gene (Internal Sample Control) in a Porcine Reproductive and Respiratory Syndrome Virus RT-qPCR by Berenice Munguía-Ramírez, Betsy Armenta-Leyva, Alexandra Henao-Díaz, Fangshu Ye, David H. Baum, Luis G. Giménez-Lirola, Jeffrey J. Zimmerman

“…In Study 2, the constancy of ISC detection over time (≥42 days) was assessed in oral fluid (<i>n</i> = 130), serum (<i>n</i> = 215), and feces (<i>n</i> = 132) collected from individual pigs of known PRRSV status. In Study 3, serum (<i>n</i> = 150), oral fluid (<i>n</i> = 150), and fecal samples (<i>n</i> = 75 feces, 75 fecal swabs) from commercial herds were used to establish ISC reference limits. …”
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Non-Invasive Sampling in the Aspect of African Swine Fever Detection—A Risk to Accurate Diagnosis by Marek Walczak, Anna Szczotka-Bochniarz, Jacek Żmudzki, Małgorzata Juszkiewicz, Krzesimir Szymankiewicz, Krzysztof Niemczuk, Daniel Pérez-Núñez, Lihong Liu, Yolanda Revilla

“…In this study, we analyzed data from three independent animal trials, in the framework of the detection of positive samples in different matrices (blood, sera, oral and rectal swabs) collected from nineteen domestic pigs infected with similar doses but under different scenarios, including different ASFV strains or routes of infection. …”
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The supplementation of female dogs with live yeast Saccharomyces cerevisiae var. boulardii CNCM I-1079 acts as gut stabilizer at whelping and modulates immunometabolic phenotype of... by Quentin Garrigues, Amélie Mugnier, Sylvie Chastant, Flavie Sicard, Flavie Sicard, Jean-Charles Martin, Ljubica Svilar, Ljubica Svilar, Mathieu Castex, Manuel Guillermo Ramis-Vidal, Manuel Guillermo Ramis-Vidal, Nicoletta Rovere, Laurine Michaud, Pauline David, Elodie Mansalier, Ana Rodiles, Hanna Mila, Emmanuelle Apper

“…The use of probiotics has been proposed, e.g., in pigs, to improve health status of sows, their reproductive performances and in turn, the health and performance of their progeny. …”
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Static Cold Storage with Mitochondria-Targeted Hydrogen Sulfide Donor Improves Renal Graft Function in an Ex Vivo Porcine Model of Controlled Donation-after-Cardiac-Death Kidney Tr... by George J. Dugbartey, Smriti Juriasingani, Mahms Richard-Mohamed, Andrew Rasmussen, Max Levine, Winnie Liu, Aaron Haig, Matthew Whiteman, Jacqueline Arp, Patrick P.W. Luke, Alp Sener

“…Renal pedicles of male pigs were clamped in situ for 30 min and the ureters and arteries were cannulated to mimic DCD. …”
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Astrocytes Enhance Streptococcus suis-Glial Cell Interaction in Primary Astrocyte-Microglial Cell Co-Cultures by Jana Seele, Roland Nau, Chittappen K. Prajeeth, Martin Stangel, Peter Valentin-Weigand, Maren Seitz

“…Streptococcus (S.) suis infections are the most common cause of meningitis in pigs. Moreover, S. suis is a zoonotic pathogen, which can lead to meningitis in humans, mainly in adults. …”
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