Development of a Multispecies Double-Antigen Sandwich ELISA Using N and RBD Proteins to Detect Antibodies against SARS-CoV-2 by Maritza Cordero-Ortiz, Mónica Reséndiz-Sandoval, Freddy Dehesa-Canseco, Mario Solís-Hernández, Jahir Pérez-Sánchez, Carlos Martínez-Borges, Verónica Mata-Haro, Jesús Hernández

“…After confirming that double-antigen sandwich ELISA with both RBD and N proteins had the best performance (AUC= 88%), the cutoff was adjusted using positive and negative samples from cats, humans (<i>n</i> = 32) and guinea pigs (<i>n</i> = 3). The use of samples from tigers (<i>n</i> = 2) and rats (<i>n</i> = 51) showed good agreement with the results previously obtained using the microneutralization test. …”
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Update on the Phylodynamics of SADS-CoV by Fabio Scarpa, Daria Sanna, Ilenia Azzena, Piero Cossu, Marta Giovanetti, Domenico Benvenuto, Elisabetta Coradduzza, Ivailo Alexiev, Marco Casu, Pier Luigi Fiori, Massimo Ciccozzi

“…The direct progenitor of SADS-CoV is so far unknown and, although it is well known that horseshoe bats are reservoirs for <i>Rhinolophus bat coronavirus HKU2-like</i> (HKU2-like CoVs), the transmission path from bats to pigs is still unclear. The discrepancies in the phylogenetic position of rodent CoV, when different molecular markers were considered, corroborate the recombination hypothesis, suggesting that wild rats, which are frequent in farms, may have played a key role. …”
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Laparoscopic renal surgery using multi degree-of-freedom articulating laparoscopic instruments in a porcine model by Jong Keun Kim, Bosik Kang, Yu Seon Kim, Yujin Yun, Myoung Jin Jang, Donghwan Bae, Dalsan You

“…Materials and Methods: Nine female Yorkshire pigs were equally divided into three groups. The three groups were compared at each surgical step in terms of objective and subjective parameters. …”
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African swine fever virus – variants on the rise by Jan H. Forth, Sten Calvelage, Melina Fischer, Jan Hellert, Julia Sehl-Ewert, Hanna Roszyk, Paul Deutschmann, Adam Reichold, Martin Lange, Hans-Hermann Thulke, Carola Sauter-Louis, Dirk Höper, Svitlana Mandyhra, Maryna Sapachova, Martin Beer, Sandra Blome

“…This virus, causing a severe haemorrhagic disease (African swine fever) with very high lethality rates in wild boar and domestic pigs, has demonstrated a remarkably high genetic stability for over 10 years. …”
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Immunobiotic <i>Ligilactobacillus salivarius</i> FFIG58 Confers Long-Term Protection against <i>Streptococcus pneumoniae</i> by Mariano Elean, Fernanda Raya Tonetti, Kohtaro Fukuyama, Luciano Arellano-Arriagada, Fu Namai, Yoshihito Suda, Nadia Gobbato, Keita Nishiyama, Julio Villena, Haruki Kitazawa

“…Previously, we isolated potentially probiotic <i>Ligilactobacillus salivarius</i> strains from the intestines of wakame-fed pigs. The strains were characterized based on their ability to modulate the innate immune responses triggered by the activation of Toll-like receptor (TLR)-3 or TLR4 signaling pathways in intestinal mucosa. …”
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Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus <i>CD2v</i> and <i>p54</i> by Jianhui Wei, Chuancheng Liu, Xinyan He, Bilal Abbas, Qi Chen, Zhaolong Li, Zhihua Feng

“…African swine fever (ASF) leads to high mortality in domestic pigs and wild boar, and it is caused by the African swine fever virus (ASFV). …”
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The Effects of Temperature Management on Brain Microcirculation, Oxygenation and Metabolism by Katia Donadello, Fuhong Su, Filippo Annoni, Sabino Scolletta, Xinrong He, Lorenzo Peluso, Leonardo Gottin, Enrico Polati, Jacques Creteur, Olivier De Witte, Jean-Louis Vincent, Daniel De Backer, Fabio Silvio Taccone

“…We studied the time course of these variables in a healthy swine model.Methods: Fifteen invasively monitored, mechanically ventilated pigs were allocated to sham procedure (normothermia, NT; <i>n</i> = 5), cooling (hypothermia, HT, <i>n</i> = 5) or cooling with controlled oxygenation (HT-Oxy, <i>n</i> = 5). …”
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Dietary lactate supplementation can alleviate DSS-induced colitis in piglets by Mingyu Wang, Zequn Fan, Daiwen Chen, Bing Yu, Jun He, Jie Yu, Xiangbing Mao, Zhiqing Huang, Yuheng Luo, Junqiu Luo, Hui Yan, Ping Zheng

“…The variations in colonic lactate in piglets with DSS-induced colitis were investigated in Experiment 1 (Exp.1). Thirty weaned pigs were allotted into three groups and sampled at different stages of DSS-induced colitis (days 0, 5, and 7). …”
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HSP90AB1 Is a Host Factor Required for Transmissible Gastroenteritis Virus Infection by Daili Song, Yujia Zhao, Ying Sun, Yixiao Liang, Rui Chen, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Qigui Yan, Xinfeng Han, Sanjie Cao, Xiaobo Huang

“…Transmissible gastroenteritis virus (TGEV) is an important swine enteric coronavirus causing viral diarrhea in pigs of all ages. Currently, the development of antiviral agents targeting host proteins to combat viral infection has received great attention. …”
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Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China by Mingliu Wang, Pengcheng Du, Jianping Wang, Ruiting Lan, Jun Huang, Ming Luo, Yan Jiang, Jun Zeng, Yi Quan, Zhaohui Shi, Han Zheng

“…Serotype 2 and sequence type (ST) 1 are the most frequently reported strains in both infected humans and pigs. ST7 is only endemic to China, and it was responsible for outbreaks in 1998 and 2005 in China. …”
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Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies by Yuanyuan Tian, Yuanyuan Tian, Chao Liang, Chao Liang, Jingming Zhou, Jingming Zhou, Fanglin Sun, Fanglin Sun, Yankai Liu, Yankai Liu, Yumei Chen, Yumei Chen, Xifang Zhu, Xifang Zhu, Hongliang Liu, Hongliang Liu, Peiyang Ding, Peiyang Ding, Enping Liu, Enping Liu, Ying Zhang, Ying Zhang, Sixuan Wu, Sixuan Wu, Aiping Wang, Aiping Wang, Aiping Wang

“…African swine fever (ASF) is a viral disease caused by the African swine fever virus that can be highly transmitted and lethal in domestic pigs. In the absence of a vaccine, effective diagnosis is critical for minimizing the virus’s spread. …”
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Effects of Iron Deficiency on Serum Metabolome, Hepatic Histology, and Function in Neonatal Piglets by Zhenglin Dong, Dan Wan, Huansheng Yang, Guanya Li, Yiming Zhang, Xihong Zhou, Xin Wu, Yulong Yin

“…Furthermore, expressions of cyp7a1 and the urea cycle enzyme (ornithinetranscarbamoylase and argininosuccinate synthetase) were significantly increased in iron deficient pigs (<i>p</i> < 0.05). The present experimental results indicated that neonatal piglets without iron supplementation drop to borderline anemia within 8 days after birth. …”
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Neuroanatomical and cellular degeneration associated with a social disorder characterized by new ritualistic belief systems in a TDP-C patient vs. a Pick patient by Daniel T. Ohm, Daniel T. Ohm, Emma Rhodes, Alejandra Bahena, Noah Capp, MaKayla Lowe, Philip Sabatini, Winifred Trotman, Christopher A. Olm, Jeffrey Phillips, Karthik Prabhakaran, Katya Rascovsky, Lauren Massimo, Corey McMillan, James Gee, M. Dylan Tisdall, Paul A. Yushkevich, Edward B. Lee, Murray Grossman, David J. Irwin, David J. Irwin

“…The FTLD-TDP patient developed a complex set of values centered on positivity and associated with specific physical and behavioral features of pigs, while the FTLD-tau patient developed compulsive, goal-directed behaviors related to general themes of positivity and spirituality. …”
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Evaluation of the Presence of ASFV in Wolf Feces Collected from Areas in Poland with ASFV Persistence by Maciej Szewczyk, Krzysztof Łepek, Sabina Nowak, Małgorzata Witek, Anna Bajcarczyk, Korneliusz Kurek, Przemysław Stachyra, Robert W. Mysłajek, Bogusław Szewczyk

“…During last few years, it has spread among populations of wild boars and pigs in countries of Eastern and Central Europe, causing huge economical losses. …”
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The neurophysiological effect of mild hypothermia in gyrencephalic brains submitted to ischemic stroke and spreading depolarizations by Roberto Díaz-Peregrino, Modar Kentar, Modar Kentar, Carlos Trenado, Carlos Trenado, Renán Sánchez-Porras, Pablo Albiña-Palmarola, Pablo Albiña-Palmarola, Pablo Albiña-Palmarola, Francisco L. Ramírez-Cuapio, Daniel San-Juan, Andreas Unterberg, Johannes Woitzik, Edgar Santos, Edgar Santos

“…ObjectiveCharacterize the neurophysiological effects of mild hypothermia on stroke and spreading depolarizations (SDs) in gyrencephalic brains.MethodsLeft middle cerebral arteries (MCAs) of six hypothermic and six normothermic pigs were permanently occluded (MCAo). Hypothermia began 1 h after MCAo and continued throughout the experiment. …”
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Evaluation of transcutaneous near-infrared spectroscopy for early detection of cardiac arrest in an animal model by Katharina Raschdorf, Arman Mohseni, Kaavya Hogle, Amanda Cheung, Kitty So, Neda Manouchehri, Mahsa Khalili, Saud Lingawi, Brian Grunau, Calvin Kuo, Jim Christenson, Babak Shadgan

“…We investigated transcutaneous NIRS as a method to detect pentobarbital-induced changes during cardiac arrest in eight Yucatan miniature pigs. NIRS measurements during cardiac arrest were compared to invasively acquired carotid blood pressure and partial oxygen pressure (PO2) of spinal cord tissues. …”
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N-Glycolylneuraminic Acid in Animal Models for Human Influenza A Virus by Cindy M. Spruit, Nikoloz Nemanichvili, Masatoshi Okamatsu, Hiromu Takematsu, Geert-Jan Boons, Robert P. de Vries

“…Animal models that are currently most often used to study human influenza are mice and ferrets. Additionally, guinea pigs, cotton rats, Syrian hamsters, tree shrews, domestic swine, and non-human primates (macaques and marmosets) are discussed. …”
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Immunogenicity and efficacy of serogroup A and D bacterins against Pasteurella multocida in mice by Li-jun Guan, Li-jun Guan, Jin-qian Yang, Qing-yuan Xu, Yi-fan Feng, Xi-chen Zhang, Bo Tang, Zhan-qin Zhao

“…IntroductionPasteurella multocida is a widespread respiratory pathogen in pigs, causing swine pneumonia and atrophic rhinitis, and the capsular serogroups A and D are the main epidemic serogroups in infected animals. …”
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Benchmarking DNA methylation analysis of 14 alignment algorithms for whole genome bisulfite sequencing in mammals by Wentao Gong, Xiangchun Pan, Dantong Xu, Guanyu Ji, Yifei Wang, Yuhan Tian, Jiali Cai, Jiaqi Li, Zhe Zhang, Xiaolong Yuan

“…Herein, based on the real and simulated WGBS data of 14.77 billion reads, we undertook 936 mappings to benchmark and evaluate 14 wildly utilized alignment algorithms from reads mapping to biological interpretation in humans, cattle and pigs: Bwa-meth, BSBolt, BSMAP, Walt, Abismal, Batmeth2, Hisat_3n, Hisat_3n_repeat, Bismark-bwt2-e2e, Bismark-his2, BSSeeker2-bwt, BSSeeker2-soap2, BSSeeker2-bwt2-e2e and BSSeeker2-bwt2-local. …”
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Effects of canagliflozin on myocardial microvascular density, oxidative stress, and proteomic profile by Sharif A. Sabe, Cynthia M. Xu, Mohamed Sabra, Dwight D. Harris, Mark Broadwin, Krishna G. Bellam, Debolina Banerjee, Anny Usheva, M. Ruhul Abid, Frank W. Sellke

“…Methods: Yorkshire swine underwent placement of an ameroid constrictor to the left circumflex artery. Two weeks later, pigs received no drug (CON, n = 8) or 300 mg CANA daily (n = 8). …”
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