Establishment and assessment of pancreas preservation using oxygenated hypothermic machine perfusion by Hakim, G

“…</p> <p><strong>Methods:</strong> Pancreases were retrieved after cold preservation solution flush from Sus scrofa domesticus pigs sourced at an abattoir, in a DCD model. Pancreases were cold preserved with Static Cold Storage (n=5), Hypothermic Machine Perfusion (Waves machine) (n=4), or with Hypothermic Oxygenated Pulsatile Perfusion (n=4) (medical air 300ml/min). …”

Characterisation of buffalo (bubalus bubalis) bone gelatin extracted using acid and papain pre-treatment by Muhammad Yazid Samatra, Nor Qhairul Izzreen Mohd Noor, Umi Hartina Mohamad Razali, Sharifudin Md. Shaarani

“…There have been substantial efforts to develop gelatin alternative sources to replace porcine because eating pigs and its derivatives is strictly prohibited in Halal and Kosher diets. …”
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Postbiotic metabolites produced by Lactobacillus plantarum strains exert selective cytotoxicity effects on cancer cells by Chuah, Li Oon, Foo, Hooi Ling, Loh, Teck Chwen, Mohammed Alitheen, Noorjahan Banu, Yeap, Swee Keong, Abdul Mutalib, Nur Elina, Abdul Rahim, Raha, Yusoff, Khatijah

“…Background: Lactobacillus plantarum, a major species of Lactic Acid Bacteria (LAB), are capable of producing postbiotic metabolites (PM) with prominent probiotic effects that have been documented extensively for rats, poultry and pigs. Despite the emerging evidence of anticancer properties of LAB, very limited information is available on cytotoxic and antiproliferative activity of PM produced by L. plantarum. …”
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The development of a highly sensitive and quantitative SARS-CoV-2 rapid antigen test applying newly developed monoclonal antibodies to an automated chemiluminescent flow-through me... by Kengo Nishimura, Hiroaki Kitazawa, Takashi Kawahata, Kosuke Yuhara, Takahiro Masuya, Toshihiro Kuroita, Kentarou Waki, Seiichi Koike, Masaharu Isobe, Nobuyuki Kurosawa

“…Results To develop a highly sensitive and accurate Ag-RDT, 90 monoclonal antibodies were raised from guinea pigs immunized with SARS CoV-2 nucleocapsid protein (CoV-2-NP). …”
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Genetic diversity of Toxoplasma gondii isolates obtained from free-living wild birds rescued in Southeastern Brazil by W.M.F. Rêgo, J.G.L. Costa, R.C.A. Baraviera, L.V. Pinto, G.L. Bessa, R.E.N. Lopes, J.A.G. Silveira, R.W.A. Vitor

“…Recent studies carried out in Brazil have shown that strains from the same Toxoplasma gondii genotype can infect humans, domestic animals (dogs and cats) and animals slaughtered for human consumption (pigs, sheep, goats, and chickens), suggesting a common infection route. …”
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Autologous Umbilical Cord Blood–Derived Mononuclear Cell Therapy Promotes Cardiac Proliferation and Adaptation in a Porcine Model of Right Ventricle Pressure Overload by Saji Oommen, Susana Cantero Peral, Muhammad Y. Qureshi, Kimberly A. Holst, Harold M. Burkhart, Matthew A. Hathcock, Walter K. Kremers, Emma B. Brandt, Brandon T. Larsen, Joseph A. Dearani, Brooks S. Edwards, Joseph J. Maleszewski, Timothy J. Nelson

“…To determine the clinical relevance of autologous umbilical cord blood–derived mononuclear cells (UCB-MNCs) as a therapy to augment cardiac adaptation following surgical management of congenital heart disease, a validated model system of right ventricular pressure overload due to pulmonary artery banding (PAB) in juvenile pigs has been employed. PAB in a juvenile porcine model and intramyocardial delivery of UCB-MNCs was evaluated in three distinct 12-week studies utilizing serial cardiac imaging and end-of-study pathology evaluations. …”
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The AnimalAssociatedMetagenomeDB reveals a bias towards livestock and developed countries and blind spots in functional-potential studies of animal-associated microbiomes by Anderson Paulo Avila Santos, Muhammad Kabiru Nata’ala, Jonas Coelho Kasmanas, Alexander Bartholomäus, Tina Keller-Costa, Stephanie D. Jurburg, Tamara Tal, Amélia Camarinha-Silva, João Pedro Saraiva, André Carlos Ponce de Leon Ferreira de Carvalho, Peter F. Stadler, Danilo Sipoli Sanches, Ulisses Rocha

“…From the collected metagenomes, 51.1% were recovered from animals associated with medical research or grown for human consumption (i.e., mice, rats, cattle, pigs, and poultry). Further, we observed an over-representation of animals collected in temperate regions (89.2%) and a lower representation of samples from the polar zones, with only 11 samples in total. …”
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Screening of Virulence-Related Transcriptional Regulators in <i>Streptococcus suis</i> by Liang Liu, Qiang Zhang, Zhongmin Xu, Bo Chen, Anding Zhang, Xiaomei Sun, Meilin Jin

“…<i>Streptococcus suis</i> (<i>S.suis</i>) is an important zoonotic pathogen that causes many severe diseases in pigs and humans. Virulence-related transcriptional regulators have been widely reported in pathogenic microorganisms, but only a few have been identified in <i>S.suis</i>. …”
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Lysine Distinctively Manipulates Myogenic Regulatory Factors and Wnt/Ca²⁺ Pathway in Slow and Fast Muscles, and Their Satellite Cells of Postnatal Piglets by Xiaofan Wang, Xiaoyin Zong, Mao Ye, Chenglong Jin, Tao Xu, Jinzeng Yang, Chunqi Gao, Xiuqi Wang, Huichao Yan

“…Thirty 28-day-old weaned piglets with similar body weights were subjected to three diet regimens: control group (d 0–28: 1.31% Lys, n = 12), Lys-deficient group (d 0–28: 0.83% Lys, n = 12), and Lys rescue group (d 0–14: 0.83% Lys; d 15–28: 1.31% Lys, n = 6). Pigs on d 15 and 29 were selectively slaughtered for muscular parameters evaluation. …”
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Lumbar Interbody Fusion Conducted on a Porcine Model with a Bioresorbable Ceramic/Biopolymer Hybrid Implant Enriched with Hyperstable Fibroblast Growth Factor 2 by Milan Krticka, Ladislav Planka, Lucy Vojtova, Vladimir Nekuda, Premysl Stastny, Radek Sedlacek, Adam Brinek, Michaela Kavkova, Eduard Gopfert, Vera Hedvicakova, Michala Rampichova, Leos Kren, Kvetoslava Liskova, Daniel Ira, Jana Dorazilová, Tomas Suchy, Tomas Zikmund, Jozef Kaiser, David Stary, Martin Faldyna, Martin Trunec

“…Twenty-four experimental pigs underwent L2/3 discectomy with implantation of either the tricortical iliac crest bone autograft or the bioresorbable hybrid implant (BHI) followed by lateral intervertebral fixation. …”
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A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibod... by Shubin Li, Ming Qiu, Shuai Li, Chen Li, Hong Lin, Yuejia Qiu, Wenhao Qi, Binghui Feng, Meng Cui, Shuai Yang, Wanglong Zheng, Shaobin Shang, Kegong Tian, Jianzhong Zhu, Yu Lu, Nanhua Chen

“…The number of SD1291-strain-specific IFNγ-secreting cells was similar in ORF2-6-CON-inoculated and rHLJB1-inoculated pigs. Overall, our findings support that the Marc-145-adapted ORF2-6-CON can trigger Tfh cell and heterologous nAb responses to confer improved cross-protection and may serve as a candidate strain for the development of a cross-protective PRRSV1 vaccine.…”
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A triplex crystal digital PCR for the detection of genotypes I and II African swine fever virus by Kaichuang Shi, Kaichuang Shi, Kaichuang Shi, Xinxiu Qian, Yuwen Shi, Haina Wei, Yi Pan, Feng Long, Qingan Zhou, Shenglan Mo, Liping Hu, Zongqiang Li

“…African swine fever (ASF) is a highly contagious and lethal viral disease that causes severe hemorrhagic fever in pigs. It keeps spreading around the world, posing a severe socioeconomic risk and endangering biodiversity and domestic food security. …”
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Comparison of Two Generations of Thoracic Aortic Stent Grafts and Their Impact on Aortic Stiffness in an Ex Vivo Porcine ModelWhat this paper adds by Tim J. Mandigers, Michele Conti, Sara Allievi, Francesca Dedola, Daniele Bissacco, Daniele Bianchi, Stefania Marconi, Maurizio Domanin, Joost A. Van Herwaarden, Ferdinando Auricchio, Santi Trimarchi

“…Thoracic aortas of young healthy pigs were harvested and connected to the mock circulatory loop. …”
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Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc by Han-Jin Oh, Yei-Ju Park, Jae Hyoung Cho, Min-Ho Song, Bon-Hee Gu, Won Yun, Ji-Hwan Lee, Ji-Seon An, Yong-Ju Kim, Jun-Soeng Lee, Sheena Kim, Hyeri Kim, Eun Sol Kim, Byoung-Kon Lee, Byeong-Woo Kim, Hyeun Bum Kim, Jin-Ho Cho, Myung-Hoo Kim

“…In conclusion, low nano-ZnO levels have similar effects on nutrient digestibility, fecal microflora, and intestinal immune profiles in weaning pigs; thus, nano-ZnO could be used as a ZnO alternative for promoting ZnO utilization and intestinal immunity.…”
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Phenotypic and Genotypic Characterization of Veterinary <i>Vibrio cincinnatiensis</i> Isolates by Claudia Jäckel, Jens Andre Hammerl, Huynh-Huong-Thao Arslan, Cornelia Göllner, Nicole vom Ort, Karin Taureck, Eckhard Strauch

“…The isolates were mostly recovered from abortion material of pigs, cattle, and horse (amnion or fetuses). One isolate was from a goose. …”
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A humoral diagnostic test outperforms cellular tests in a farm with a latent tuberculosis outbreak caused by a new Mycobacterium tuberculosis complex spoligotype that affected shee... by Ramon A. Juste, Leire Fernández-Veiga, Miguel Fuertes, Ignacio Fernández-Ortiz de Murua, Guillermo Cardona, Maria V. Geijo, Joseba M. Garrido, Iker A. Sevilla

“…M. caprae, a member of the complex which is close to M. bovis, is emerging and affects several different hosts that include goats, cattle, sheep, pigs, rabbits, wild boar, red deer, foxes and also humans. …”
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Development and Application of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Detection and Differentiation of EP402R-Deleted and Wild-Type African Swine Fever Virus by Junhai Zhu, Junhai Zhu, Junhai Zhu, Junhai Zhu, Junhai Zhu, Weijun Jian, Weijun Jian, Weijun Jian, Weijun Jian, Yifan Huang, Yifan Huang, Yifan Huang, Yifan Huang, Qi Gao, Qi Gao, Qi Gao, Qi Gao, Fei Gao, Fei Gao, Fei Gao, Fei Gao, Huahan Chen, Huahan Chen, Huahan Chen, Huahan Chen, Guihong Zhang, Guihong Zhang, Guihong Zhang, Guihong Zhang, Ming Liao, Ming Liao, Ming Liao, Ming Liao, Ming Liao, Wenbao Qi, Wenbao Qi, Wenbao Qi, Wenbao Qi, Wenbao Qi

“…ASF has been raging in China for 3 years, and recently EP402R-deleted ASFV strains emerged, showing sub-acute or chronic symptoms in pigs and providing novel difficulties to monitor and control the disease as EP402R-deleted strains possess no hemadsorption (HAD) ability. …”
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<i>Limosilactobacillus fermentum</i> 3872 That Produces Class III Bacteriocin Forms Co-Aggregates with the Antibiotic-Resistant <i>Staphylococcus aureus</i> Strains and Induces The... by Vyacheslav M. Abramov, Igor V. Kosarev, Andrey V. Machulin, Tatiana V. Priputnevich, Evgenia I. Deryusheva, Ekaterina L. Nemashkalova, Irina O. Chikileva, Tatiana N. Abashina, Alexander N. Panin, Vyacheslav G. Melnikov, Nataliya E. Suzina, Ilia N. Nikonov, Marina V. Selina, Valentin S. Khlebnikov, Vadim K. Sakulin, Vladimir A. Samoilenko, Alexey B. Gordeev, Gennady T. Sukhikh, Vladimir N. Uversky, Andrey V. Karlyshev

“…The sensitivity of the antibiotic-resistant <i>S. aureus</i> collection strains and freshly isolated antibiotic-resistant strains was tested using samples from women with lactation mastitis; the human nasopharynx and oral cavity; the oropharynx of pigs; and the cows with a diagnosis of clinical mastitis sensitive to the lytic action of the LF3872 strain producing BLF3872. …”
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M2 Macrophage‐Derived sEV Regulate Pro‐Inflammatory CCR2+ Macrophage Subpopulations to Favor Post‐AMI Cardiac Repair by Lan Li, Jiasong Cao, Sheng Li, Tianyi Cui, Jingyu Ni, Han Zhang, Yan Zhu, Jingyuan Mao, Xiumei Gao, Adam C. Midgley, Meifeng Zhu, Guanwei Fan

“…Here, the authors harvested M2 macrophage‐derived sEV (M2EV) from M2‐polarized bone‐marrow‐derived macrophages for intramyocardial injection and recapitulation of sEV‐mediated anti‐inflammatory activity in ischemic‐reperfusion (I/R) injured hearts. Rats and pigs received sham surgery; I/R without treatment; or I/R with autologous M2EV treatment. …”
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Transcriptome and metabolome after porcine hemodynamic-directed CPR compared with standard CPR and sham controls by Kumaran Senthil, Marco M. Hefti, Larry N. Singh, Ryan W. Morgan, Constantine D. Mavroudis, Tiffany Ko, Hunter Gaudio, Vinay M. Nadkarni, Johannes Ehinger, Robert A. Berg, Robert M. Sutton, Francis X. McGowan, Todd J. Kilbaugh

“…Subjects: Four-week-old female piglets (8–11 kg). Interventions: Pigs (1-month-old), three groups: 1) HD-CPR (compression depth to systolic BP 90 mmHg, vasopressors to coronary perfusion pressure 20 mmHg); 2) Std-CPR and 3) shams (no CPR). …”
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