Use of Transient Transfection for cGMP Manufacturing of eOD-GT8 60mer, a Self-Assembling Nanoparticle Germline-Targeting HIV-1 Vaccine Candidate by Sharma, Vaneet K., Menis, Sergey, Brower, Evan T., Sayeed, Eddy, Ackland, Jim, Lombardo, Angela, Cottrell, Christopher A., Torres, Jonathan L., Hassell, Thomas, Ward, Andrew B., Tsvetnitsky, Vadim, Schief, William R.

“…Production was carried out via transient expression in the human embryonic kidney 293 (HEK293) cell line followed by a combination of purification techniques. A large-scale cGMP (200 L) production run yielded 354 mg of the purified eOD-GT8 60mer drug product material, which was formulated at 1 mg/mL in 10% sucrose in phosphate-buffered saline (PBS) at pH 7.2. …”
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Synthesized tin-activated carbon adsorbent for purer and cheaper hydrogen by Iyuke, S.E.

“…Since the catalyst can only tolerate CO of less than 100 ppm, it is then expedient to introduce a purification system to reduce the CO content to the required concentration. …”
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Conformational design characterization of a truncated diamine oxidase by Razali, Nur Nadia

“…High purification yield of 88% was obtained for mini protein fusion tagged (His-tagged + mature mini protein). …”
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Synthesis and characterization of a novel biodegradable polylactide. by Cheong, Qi Li.

“…A series of crosslinked PLA samples were prepared using different reaction conditions and purification techniques. Tensile test demonstrated improved mechanical properties in the synthesized polymers. …”
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Watch and learn - laboratory practical made easy. by Wong, Mei Kee.

“…The first of its kind in SBS, this video will endeavour to guide students through the experiment on GST protein extraction and purification.…”
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Polymers at Nanoscale / by He, Jie, editor 655466, Wang, Xin, editor 655467, World Scientific (Online service) 655444

“…It showcases a wide range of advanced applications of polymer nanoparticles in several fields that include pharmaceutics (drug and nucleotide delivery), biomedicals (bioimaging, diagnosis, and therapeutics), energy (batteries and solar cells) and environmental (catalysis and water purification). This book is enriched with a comprehensive range of content, incorporating synthesis, properties and applications in polymeric nanoparticles that will serve as a suitable beginner guide and survey book in polymer nanomaterials, as well as a useful tool for graduate students, scientists and practitioners in related fields or industries such as chemistry, materials science and engineering, nanomaterials, energy storage and conversion devices, and biomedicine"--…”
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Energizing solar still efficiency with eco-friendly coconut shell biochar enhanced organic phase change material by Rajamony, Reji Kumar, Suraparaju, Subbarama Kousik, Kalidasan, B., Yadav, Aman, Kumar Pandey, Adarsh Kumar, Nurhanis Sofiah, Abd Ghafar, Siaw Paw, Johnny Koh, Fatehmulla, Amanullah, Samykano, Mahendran, Saidur, Rahman Md

“…These systems utilize solar energy for the processes of evaporation and condensation, offering a sustainable and environmentally friendly method for water purification. However, the primary drawback of conventional SS lies in its relatively low production capacity, constrained by daylight hours and limited thermal efficiency. …”
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Enhancing fouling mitigation of submerged flat-sheet membranes by vibrating 3D-spacers by Tan, Yong Zen, Mao, Ziming, Zhang, Yanjun, Tan, Wen See, Chong, Tzyy Haur, Wu, Bing, Chew, Jia Wei

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Synthetic approaches to all-porphyrin nanorings by Field, J

“…The Suzuki strategy was successful in synthesizing a range of different sized all-porphyrin nanorings, but the reaction was highly sensitive to a range of difficult-to-control factors, as well as significant purification issues. This ultimately resulted in this method being insufficiently repeatable and scalable.…”

Water reclaimation and reuse using photocatalyst : review and preliminary experiment by Ng, Guan Si.

“…Titanium dioxide (TiO2), which is one of the most basic materials in our daily life, has emerged as an excellent photocatalyst material for environmental purification. TiO2 is close to being an ideal photocatalyst because it is relatively inexpensive, highly stable chemically and the photogenerated holes are highly oxidizing. …”
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Synthesis of hydrogel particles for disaster relief application by Fan, Hao

“…Portable and easy-to-use water purification method is needed in regions impacted by disasters to provide drinking water to the affected population. …”
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Hybrid antibacterial agents. by Lin, Jiawen.

“…However, further progress was impeded by limited yield and purification difficulty from solid- and liquid-phase peptide synthesis method respectively. …”
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The role of bacteriophages in an aerobic biogranulation system by A R Balamurugan

“…The filtration process for the separation of bacteriophage from the AGS system was also optimized and automated to allow for more efficient and less laborious purification procedure.…”
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Water treatment using one-dimensional manganese oxide based materials by Zhang, Tong

“…In recent years, there has been a growing trend in the usage of nanomaterials to enhance water purification efficiency. One-dimensional (1D) nanostructured manganese oxides and their derivatives have attracted considerable research interest due to their large surface area, adjustable transport properties and chemical specificities. …”
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Selective and Chemical-Free Removal of Toxic Heavy Metal Cations from Water Using Shock Ion Extraction by Alkhadra, Mohammad A, Jordan, Matthew L, Tian, Huanhuan, Arges, Christopher G, Bazant, Martin Z

“…Electrochemical methods are known to have attractive features and capabilities when used for ion separations and water purification. In this study, we developed a new process called shock ion extraction (shock IX) for selective and chemical-free removal of toxic heavy metals from water. …”
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In vivo visualization of cells with fluorescent-labeled fab fragment. by Wee, Armanda Lixuan.

“…Our results showed that mouse IgG and rat IgG displayed differential susceptibility to cleavage by papain, and Protein A columns were unsuitable for the purification of Fab fragments from rat IgG. Mouse Fab fragments labeled with DyLight 650 (DL650) were found to have lower binding avidity and fluorescence intensity when compared to DL650-conjugated IgG. …”
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Hierarchically structured TIO2 nanorod spheres for clean water and energy production by Bai, Hongwei

“…Hydrothermally synthesized TiO2 nanorod spheres were applied for concurrent photocatalytic membrane filtration for water purification via a “win-win” strategy to maximize the advantages of photocatalysis and membrane filtration. …”
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Structural characterization of the full-length Hantaan virus polymerase by Keown, JR, Carrique, L, Nilsson-Payant, BE, Fodor, E, Grimes, JM

“…Here we establish the expression and purification of the polymerase from the Old World Hantaan virus and characterise the structure using Cryo-EM. …”

Discovery of stabilized functional mutants of human aquaporin 1 by Soh, Miao Yi

“…In future, we hope to characterize these mutants for increased stability as they may be useful in structural studies, drug discovery in human cancers related to AQP1 overexpression as well as in biotechnological applications, such as water purification, in particular.…”
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Advanced omniphobic membrane: fabrication via NIPS method using PVDF-HFP, TEOS, and PFDTMS by Abid, Monis, Abdul Wahab, Roswanira, Gzara, Lassaad, Baig, Nadeem, Kormin, Faridah, Ahmed Moujdin, Iqbal, Salam, Mohamed Abdel

“…Amid the global water crisis, developing innovative solutions for sustainable water purification is crucial. Membrane distillation (MD) has emerged as a pivotal method with significant potential to address these challenges effectively. …”