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    High-throughput and reliable acquisition of in vivo turnover number fuels precise metabolic engineering by Zhenghong Li, Chengyu Zhang, Zhengduo Wang, Hao Yan, Chuan Li, Zhiheng Yang, Zilong Li, Lixin Zhang, Weishan Wang

    Published 2022-03-01
    “…As synthetic biology enters the era of quantitative biology, mathematical information such as kinetic parameters of enzymes can offer us an accurate knowledge of metabolism and growth of cells, and further guidance on precision metabolic engineering. kcat, termed the turnover number, is a basic parameter of enzymes that describes the maximum number of substrates converted to products each active site per unit time. …”
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