Preclinical Head and Neck Squamous Cell Carcinoma Models for Combined Targeted Therapy Approaches by Nina Schoenwaelder, Mareike Krause, Thomas Freitag, Björn Schneider, Sarah Zonnur, Annette Zimpfer, Anne Sophie Becker, Inken Salewski, Daniel Fabian Strüder, Heiko Lemcke, Christina Grosse-Thie, Christian Junghanss, Claudia Maletzki

“…Our cell lines showed different in vitro growth kinetics, morphology, invasive potential, and radiosensitivity. All cell lines were sensitive to 5-FU, Cisplatin, and THZ1. …”
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MicroRNA Profiling in Oesophageal Adenocarcinoma Cell Lines and Patient Serum Samples Reveals a Role for miR-451a in Radiation Resistance by Frederike Butz, Ann-Kathrin Eichelmann, George C. Mayne, Tingting Wang, Isabell Bastian, Karen Chiam, Shashikanth Marri, Pamela J. Sykes, Bas P. Wijnhoven, Eelke Toxopeus, Michael Z. Michael, Christos S. Karapetis, Richard Hummel, David I. Watson, Damian J. Hussey

“…Inhibition of miR-451a in the radiation resistant OAC cell line OE19 increased radiosensitivity (Survival Fraction 73% vs. 87%, <i>p</i> = 0.0003), and altered RNA expression. …”
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Toward a Transportable Cell Culture Platform for Evaluating Radiotherapy Dose Modifying Factors by Nicholas Carlson, Carrie D. House, Mauro Tambasco

“…Additionally, we measured the clonogenic viability of the cells to characterize how the duration of the TCCP at room temperature affected their radiosensitivity at doses up to 16 Gy. We found that <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>(</mo><mn>78</mn><mo>±</mo><mn>2</mn><mo>)</mo><mo>%</mo></mrow></semantics></math></inline-formula> of MDA-MB-231 cells were successfully recovered after being kept at room temperature for three days in 50% FBS in medium-supplemented gelatin at hypoxia (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>0.4</mn><mo>±</mo><mn>0.1</mn><mo>)</mo><mo>%</mo></mrow></semantics></math></inline-formula> pO₂, while metabolic and clonogenic viabilities as measured by ATP luminescence and colony formation were found to be <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>(</mo><mn>58</mn><mo>±</mo><mn>5</mn><mo>)</mo><mo>%</mo></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>(</mo><mn>57</mn><mo>±</mo><mn>4</mn><mo>)</mo></mrow></semantics></math></inline-formula>%, respectively. …”
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Demographic, clinical, immunological, and molecular features of iranian national cohort of patients with defect in DCLRE1C gene by Soodeh Ghadimi, Mahnaz Jamee, Hassan Abolhassani, Nima Parvaneh, Nima Rezaei, Samaneh Delavari, Mahnaz Sadeghi-Shabestari, Sedigheh Rafiei Tabatabaei, Alireza Fahimzad, Shahnaz Armin, Zahra Chavoshzadeh, Samin Sharafian

“…Impaired DNA repair and block in early adaptive immunity maturation results in T-B-NK+ immunodeficiency associated with radiosensitivity. Recurrent infections early in life are the main characteristic of Artemis patients. …”
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The Role of SOX2 and SOX9 in Radioresistance and Tumor Recurrence by Silvia Barbosa, Natalia Koerich Laureano, Wahyu Wijaya Hadiwikarta, Fernanda Visioli, Mahnaz Bonrouhi, Kinga Pajdzik, Cristina Conde-Lopez, Christel Herold-Mende, Gustavo Eidt, Renan Langie, Marcelo Lazzaron Lamers, Fabian Stögbauer, Jochen Hess, Ina Kurth, Adriana Jou

“…In vitro findings reveal that silencing SOX2 enhances tumor radioresistance, while SOX9 silencing enhances radiosensitivity. These discoveries lay the groundwork for further studies on the therapeutic potential of transcription factors in optimizing HNSCC treatment.…”
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STC2 activates PRMT5 to induce radioresistance through DNA damage repair and ferroptosis pathways in esophageal squamous cell carcinoma by Kan Jiang, Xin Yin, Qingyi Zhang, Jie Yin, Qiuying Tang, Mengyou Xu, Lingyun Wu, Yifan Shen, Ziyang Zhou, Hao Yu, Senxiang Yan

“…In this study, STC2 was screened out by RNA-sequencing and bioinformatics analyses as a potential prognosis predictor of ESCC radiosensitivity and then was determined to facilitate radioresistance. …”
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Sphingosine-1-Phosphate Alleviates Irradiation Induced Salivary Gland Hypofunction through Preserving Endothelial Cells and Resident Macrophages by Tao Yang, Qingguo Zhao, Meijun Hu, Simin Pan, Linying Zhang, Ruoxi Zhu, Bowen Zhou, Xuanhe Feng, Zhenhua Gao, Zhao Zhu, Yu Zhang, Liang Hu, Fei Liu, Zhaochen Shan

“…Moreover, intra-SMG-injected S1P did not affect the growth and radiosensitivity of head-and-neck cancer in a mouse model. …”
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Different Impacts of DNA-PK and mTOR Kinase Inhibitors in Combination with Ionizing Radiation on HNSCC and Normal Tissue Cells by Nina Klieber, Laura S. Hildebrand, Eva Faulhaber, Julia Symank, Nicole Häck, Annamaria Härtl, Rainer Fietkau, Luitpold V. Distel

“…The application of kinase inhibitors (KIs) in HNSCC, specifically mTOR and DNA-PK inhibitors, can increase radiosensitivity and therefore presents a promising strategy when used simultaneously with ionizing radiation (IR) in cancer treatment. …”
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CD9- and CD81-positive extracellular vesicles provide a marker to monitor glioblastoma cell response to photon-based and proton-based radiotherapy by Sara Jennrich, Martin Pelzer, Tobias Tertel, Benjamin Koska, Melanie Vüllings, Basant Kumar Thakur, Verena Jendrossek, Beate Timmermann, Bernd Giebel, Justine Rudner

“…Although secreted EVs of non-irradiated cells were not predictive for radiosensitivity, their increased EV release after irradiation correlated with the cytotoxic response to radiotherapy 72 h after irradiation. …”
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Inhibition of HIF-1α by Atorvastatin During ¹³¹I-RTX Therapy in Burkitt’s Lymphoma Model by Eun-Ho Kim, Hae Young Ko, A Ram Yu, Hyeongi Kim, Javeria Zaheer, Hyun Ji Kang, Young-Cheol Lim, Kyung Deuk Cho, Hyun-Yoo Joo, Min Kyoung Kang, Jae Jun Lee, Seung-Sook Lee, Hye Jin Kang, Sang Moo Lim, Jin Su Kim

“…The underlying mechanism of ATV involved induction of anti-angiogenesis and radiosensitivity by downregulating HIF-1α in Raji cells. …”
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Photon and Proton irradiation in Patient-derived, Three-Dimensional Soft Tissue Sarcoma Models by Siyer Roohani, Jürgen Loskutov, Jens Heufelder, Felix Ehret, Lena Wedeken, Manuela Regenbrecht, Rica Sauer, Daniel Zips, Andrea Denker, Antonia M. Joussen, Christian R. A. Regenbrecht, David Kaul

“…Conclusions Pronounced differences in radiosensitivity are evident among UPS and PLS 3D patient-derived sarcoma cell cultures which may reflect the clinical heterogeneity. …”
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Radiation Response of Murine Embryonic Stem Cells by Christine E. Hellweg, Vaibhav Shinde, Sureshkumar Perumal Srinivasan, Margit Henry, Tamara Rotshteyn, Christa Baumstark-Khan, Claudia Schmitz, Sebastian Feles, Luis F. Spitta, Ruth Hemmersbach, Jürgen Hescheler, Agapios Sachinidis

“…Cell cycle progression was determined by flow cytometry of propidium iodide-stained cells, and DNA double strand break (DSB) induction and repair by γH2AX immunofluorescence. The radiosensitivity of mESCs was slightly higher compared to the murine osteoblast cell line OCT-1. …”
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RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11 by Yarui Zhang, Guomin Ou, Zhujing Ye, Zhou Zhou, Qianlin Cao, Mengting Li, Jingdong Wang, Jianping Cao, Hongying Yang

“…Here it is identified that protein Reprimo (RPRM) downregulates ATM protein levels, resulting in impaired DNA repair and enhanced cellular radiosensitivity. Mechanistically, although primarily localized in the cytoplasm, RPRM translocates to the nucleus shortly after induced by X-irradiation, interacts with ATM and promotes its nuclear export and proteasomal degradation. …”
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<i>Dclre1c</i>-Mutation-Induced Immunocompromised Mice Are a Novel Model for Human Xenograft Research by Yixiao Bin, Sanhua Wei, Ruo Chen, Haowei Zhang, Jing Ren, Peijuan Liu, Zhiqian Xin, Tianjiao Zhang, Haijiao Yang, Ke Wang, Zhuan Feng, Xiuxuan Sun, Zhinan Chen, Hai Zhang

“…Artemis deficiency precipitates a profound immunodeficiency syndrome, marked by radiosensitivity and compromised T and B lymphocyte functionality. …”
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The Individual Effects of Cyclin-Dependent Kinase Inhibitors on Head and Neck Cancer Cells—A Systematic Analysis by Nina Schoenwaelder, Inken Salewski, Nadja Engel, Mareike Krause, Björn Schneider, Michael Müller, Christin Riess, Heiko Lemcke, Anna Skorska, Christina Grosse-Thie, Christian Junghanss, Claudia Maletzki

“…Dinaciclib, palbociclib, and THZ1 displayed anti-neoplastic activity after low-dose treatment, while the two latter substances slightly enhanced radiosensitivity. Dinaciclib decelerated wound healing, decreased invasiveness, and induced MHC-I, accompanied by high amounts of surface-bound Calreticulin. …”
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Platinum-crosslinking polymeric nanoparticle for synergetic chemoradiotherapy of nasopharyngeal carcinoma by Yuxun Ding, Xiaohui Xiao, Lingli Zeng, Qiuping Shang, Wei Jiang, Sha Xiong, Xiaohui Duan, Jun Shen, Ruibing Wang, Jinshan Guo, Yue Pan

“…Despite extensive use of radiotherapy in nasopharyngeal carcinoma (NPC) treatment because of its high radiosensitivity, there have been huge challenges in further improving therapeutic effect, meanwhile obviously reducing radiation damage. …”
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LncRNA ANRIL promotes HR repair through regulating PARP1 expression by sponging miR-7-5p in lung cancer by Zhipeng Du, Fangxiao Zhang, Lei Liu, Hui Shen, Tingting Liu, Jing Jin, Nanxi Yu, Zhijie Wan, Hang Wang, Xuguang Hu, Yuanyuan Chen, Jianming Cai

“…Blocking DNA damage repair can increase the radiosensitivity of tumor cells. Recent studies have identified long noncoding RNAs as key regulators in DNA damage repair. …”
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Morphological, Functional and Texture Analysis Magnetic Resonance Imaging Features in the Assessment of Radiotherapy-Induced Xerostomia in Oropharyngeal Cancer by Leonardo Calamandrei, Luca Mariotti, Eleonora Bicci, Linda Calistri, Eleonora Barcali, Martina Orlandi, Nicholas Landini, Francesco Mungai, Luigi Bonasera, Pierluigi Bonomo, Isacco Desideri, Leonardo Bocchi, Cosimo Nardi

“…Accordingly, texture analysis on ADC maps should be considered a useful tool to evaluate salivary gland radiosensitivity and help identify patients at risk of developing more serious xerostomia before radiation therapy is administered.…”
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The role of radiotherapy-related autophagy genes in the prognosis and immune infiltration in lung adenocarcinoma by Jingyan Gao, Fei Lu, Fei Lu, Jiawen Yan, Run Wang, Yaoxiong Xia, Li Wang, Lan Li, Li Chang, Wenhui Li

“…The colony formation assay showed that the radiosensitivity of radiation-resistant cell lines was lower than that of primary cells. …”
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A Multi-Scale and Multi-Technique Approach for the Characterization of the Effects of Spatially Fractionated X-ray Radiation Therapies in a Preclinical Model by Mariele Romano, Alberto Bravin, Alberto Mittone, Alicia Eckhardt, Giacomo E. Barbone, Lucie Sancey, Julien Dinkel, Stefan Bartzsch, Jens Ricke, Marianna Alunni-Fabbroni, Heidrun Hirner-Eppeneder, Dmitry Karpov, Cinzia Giannini, Oliver Bunk, Audrey Bouchet, Viktoria Ruf, Armin Giese, Paola Coan

“…Histology, immunohistochemistry, SAXS/WAXS and XRF allowed identification and classification of these deposits as hydroxyapatite crystals with the coexistence of Ca, P and Fe mineralization, and the multi-technique approach enabled the realization, for the first time, of the map of the differential radiosensitivity of the different brain areas treated with MRT and MB. 3D XPCI-CT datasets enabled also the quantification of tumor volumes and Ca/Fe deposits and their full-organ visualization. …”
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