Dual-fluorescent reporter for live-cell imaging of the ER during DENV infection by Lochlain Corliss, Madeline Holliday, Nicholas J. Lennemann

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HSV as A Platform for the Generation of Retargeted, Armed, and Reporter-Expressing Oncolytic Viruses by Laura Menotti, Elisa Avitabile, Valentina Gatta, Paolo Malatesta, Biljana Petrovic, Gabriella Campadelli-Fiume

“…Additionally, the insertion of mIL12 in the US1-US2 intergenic region of the HER2- or EGFRvIII-retargeted o-HSVs, and the further insertion of Gaussia Luciferase, gave rise to viable recombinants capable of secreting the cytokine and the reporter. Lastly, we engineered two known mutations in gB; they increased the ability of an HER2-retargeted recombinant to spread among murine cells. …”
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Correction: Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles. by Maria João Jacinto, João R C Trabuco, Binh V Vu, Gavin Garvey, Mohammad Khodadadi, Ana M Azevedo, Maria Raquel Aires-Barros, Long Chang, Katerina Kourentzi, Dmitri Litvinov, Richard C Willson

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Enrichment of transgene integrations by transient CRISPR activation of a silent reporter gene by Nanna S. Mikkelsen, Sabina S. Hernandez, Trine I. Jensen, Jessica L. Schneller, Rasmus O. Bak

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Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells by Tingting Xu, Madison Gilliam, Gary Sayler, Steven Ripp, Dan Close

“…By eliminating exogenous substrate, this assay provided a significant advantage over traditional reporter assays by enabling higher-throughput screening with reduced testing costs while maintaining detection accuracy.…”
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A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma by Haley R Noonan, Alexandra M Thornock, Julia Barbano, Michael E Xifaras, Chloe S Baron, Song Yang, Katherine Koczirka, Alicia M McConnell, Leonard I Zon

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Streamlined Yeast Colorimetric Reporter Activity Assays Using Scanners and Plate Readers by Ilya G. Serebriiskii, Garabet G. Toby, Erica A. Golemis

“…It is possible to augment the utility of two-hybrid systems and derivative systems such as dual-bait two-hybrid systems by adapting strategies that speed the analysis of the relative strength of a series of protein-protein associations. This report describes two simple techniques that employ either a flatbed scanner or a plate reader to quantitate the activity of colorimetric reporters such as LacZ or GusA commonly used in two-hybrid approaches.…”
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Functional dissection of neural circuitry using a genetic reporter for fMRI by Ghosh, Souparno, Li, Nan, Schwalm, Miriam, Bartelle, Benjamin B, Xie, Tianshu, Daher, Jade I, Singh, Urvashi D, Xie, Katherine, DiNapoli, Nicholas, Evans, Nicholas B, Chung, Kwanghun, Jasanoff, Alan

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Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa by Borlee, Bradley R., Nielsen, Thomas E., Parsek, Matthew R., Rybtke, Morten Theil, Murakami, Keiji, Irie, Yasuhiko, Hentzer, Morten, Givskov, Michael, Tolker-Nielsen, Tim

“…We have created cyclic di-GMP level reporters by transcriptionally fusing the cyclic di-GMP-responsive cdrA promoter to genes encoding green fluorescent protein. …”
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An activatable NIR-II fluorescent reporter for in vivo imaging of amyloid-β plaques by Miao, Jia, Miao, Minqian, Jiang, Yue, Zhao, Min, Li, Qing, Zhang, Yuan, An, Yi, Pu, Kanyi, Miao, Qingqing

“…Fluorescence imaging in the second near-infrared (NIR-II) window holds great promise for in vivo visualization of amyloid-β (Aβ) pathology, which can facilitate characterization and deep understanding of Alzheimer's disease (AD); however, it has been rarely exploited. Herein, we report the development of NIR-II fluorescent reporters with a donor-π-acceptor (D-π-A) architecture for specific detection of Aβ plaques in AD-model mice. …”
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A tandem-locked fluorescent NETosis reporter for the prognosis assessment of cancer immunotherapy by Cheng, Penghui, He, Shasha, Zhang, Chi, Liu, Jing, Pu, Kanyi

“…Non-invasive real-time imaging is thus imperative for prognosis of cancer immunotherapy yet remains challenging. Herein, we report a Tandem-locked NETosis Reporter 1 (TNR1 ) that activates fluorescence signals only in the presence of both neutrophil elastase (NE) and cathepsin G (CTSG) for the specific imaging of NETosis. …”
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Quantitative glucose detection by surface-enhanced Raman spectroscopy using viologens as reporter. by Cheng, Chun Chieh.

“…According to previous studies, glucose has been difficult to detect by SERS due to its poor Raman cross section and its inability to absorb onto metallic surfaces. In this report, the study of indirect glucose detection via SERS is documented. …”
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Feasibility of magnetic gold nanoparticles for use as reporter In a lateral flow immunoassay by Lee, Chleo Ke Rou

“…Popular reporters like Colloidal Gold and magnetic nanobeads have high sensitivity, stability and bio-compatibility: each reporter offering a unique advantage over the other. …”
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Investigating cell type specific metabolism using GFP as a reporter protein by Rossi, M, Ratcliffe, R, Kruger, N

“…The fundamental difficulty in applying a reporter protein strategy in a multicellular organism arises from the limited amount of recombinant protein expressed by the cells.…”

Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter. by Victora, G, Schwickert, T, Fooksman, DR, Kamphorst, A, Meyer-Hermann, M, Dustin, M, Nussenzweig, M

Nestin reporter transgene labels multiple central nervous system precursor cells. by Walker, A, Goings, G, Kim, Y, Miller, R, Chenn, A, Szele, F

Rational optimization and imaging in vivo of a genetically encoded optical voltage reporter. by Sjulson, L, Miesenböck, G

“…We evaluate strategies for potential further improvement of hVOS quantitatively and derive theoretical performance limits for optical voltage reporters in general.…”

Technical Advance: Fluorescent reporter reveals insights into eomesodermin biology in cytotoxic lymphocytes. by Paley, M, Gordon, S, Bikoff, E, Robertson, E, Wherry, E, Reiner, S

“…Characterization of reporter activity in NK lineage cells also permitted identification of a novel intermediate of NK cell maturation. …”

Agrobacterium-mediated genetic transformation of Pjalaenopsis using GFP and GUS reporter genes. by Mahmood, Maziah, Chew, Yee Chern

Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection by Yan B., Wang H., Kon T., Li C.-Y.

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