Express your LOV: an engineered flavoprotein as a reporter for protein expression and purification. by Jayde A Gawthorne, L Evan Reddick, Snezhana N Akpunarlieva, Katherine S H Beckham, John M Christie, Neal M Alto, Mads Gabrielsen, Andrew J Roe

“…In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function. Specifically, we used iLOV, an enhanced and more photostable variant of LOV. …”
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A neuronal cell-based reporter system for monitoring the activity of HDAC2 by Kazuhiro Unemura, Masako Kawano, Mai Takakura, Ikuko Iwata, Kana Hyakkoku, Naotaka Horiguchi, Tomohiko Okuda, Yukinori Hirano

“…We also established the knock-in neuronal cell lines of a bioluminescence reporter gene to ID1. The knock-in cell lines showed significant reporter activity by known HDAC inhibitors and by HDAC2-knockdown but not by HDAC1-knockdown. …”
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An In Vitro Model for Acute Myeloid Leukemia Relapse Using the SORE6 Reporter by Justine Lai, Chuquan Shang, Will Chen, Iyare Izevbaye, Michael P. Chu, Irwindeep Sandhu, Joseph Brandwein, Raymond Lai, Peng Wang

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Dual-fluorescent reporter for live-cell imaging of the ER during DENV infection by Lochlain Corliss, Madeline Holliday, Nicholas J. Lennemann

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HSV as A Platform for the Generation of Retargeted, Armed, and Reporter-Expressing Oncolytic Viruses by Laura Menotti, Elisa Avitabile, Valentina Gatta, Paolo Malatesta, Biljana Petrovic, Gabriella Campadelli-Fiume

“…Additionally, the insertion of mIL12 in the US1-US2 intergenic region of the HER2- or EGFRvIII-retargeted o-HSVs, and the further insertion of Gaussia Luciferase, gave rise to viable recombinants capable of secreting the cytokine and the reporter. Lastly, we engineered two known mutations in gB; they increased the ability of an HER2-retargeted recombinant to spread among murine cells. …”
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Correction: Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles. by Maria João Jacinto, João R C Trabuco, Binh V Vu, Gavin Garvey, Mohammad Khodadadi, Ana M Azevedo, Maria Raquel Aires-Barros, Long Chang, Katerina Kourentzi, Dmitri Litvinov, Richard C Willson

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Duplex microRNAs assay based on target-triggered universal reporter hybridization by Yinan Wang, Yue Sun, Choiwan Lau, Jianzhong Lu

“…Keywords: xMAP array, Multiple microRNAs, Target-triggered, Universal reporter…”
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Enrichment of transgene integrations by transient CRISPR activation of a silent reporter gene by Nanna S. Mikkelsen, Sabina S. Hernandez, Trine I. Jensen, Jessica L. Schneller, Rasmus O. Bak

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A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity by Camilla Højland Knudsen, Camilla Højland Knudsen, Emilía S. Ásgrímsdóttir, Emilía S. Ásgrímsdóttir, Karim Rahimi, Karim Rahimi, Katherine P. Gill, Katherine P. Gill, Søs Frandsen, Søs Frandsen, Susanne Hvolbøl Buchholdt, Susanne Hvolbøl Buchholdt, Muwan Chen, Muwan Chen, Jørgen Kjems, Jørgen Kjems, Fabia Febbraro, Fabia Febbraro, Fabia Febbraro, Mark Denham, Mark Denham

“…We designed an improved monomeric red fluorescent protein reporter, GEmCherry2, for assessing CRISPR Cas9 activity and for optimizing sgRNA. …”
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Functional dissection of neural circuitry using a genetic reporter for fMRI by Ghosh, Souparno, Li, Nan, Schwalm, Miriam, Bartelle, Benjamin B, Xie, Tianshu, Daher, Jade I, Singh, Urvashi D, Xie, Katherine, DiNapoli, Nicholas, Evans, Nicholas B, Chung, Kwanghun, Jasanoff, Alan

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Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa by Borlee, Bradley R., Nielsen, Thomas E., Parsek, Matthew R., Rybtke, Morten Theil, Murakami, Keiji, Irie, Yasuhiko, Hentzer, Morten, Givskov, Michael, Tolker-Nielsen, Tim

“…We have created cyclic di-GMP level reporters by transcriptionally fusing the cyclic di-GMP-responsive cdrA promoter to genes encoding green fluorescent protein. …”
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An activatable NIR-II fluorescent reporter for in vivo imaging of amyloid-β plaques by Miao, Jia, Miao, Minqian, Jiang, Yue, Zhao, Min, Li, Qing, Zhang, Yuan, An, Yi, Pu, Kanyi, Miao, Qingqing

“…Fluorescence imaging in the second near-infrared (NIR-II) window holds great promise for in vivo visualization of amyloid-β (Aβ) pathology, which can facilitate characterization and deep understanding of Alzheimer's disease (AD); however, it has been rarely exploited. Herein, we report the development of NIR-II fluorescent reporters with a donor-π-acceptor (D-π-A) architecture for specific detection of Aβ plaques in AD-model mice. …”
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A tandem-locked fluorescent NETosis reporter for the prognosis assessment of cancer immunotherapy by Cheng, Penghui, He, Shasha, Zhang, Chi, Liu, Jing, Pu, Kanyi

“…Non-invasive real-time imaging is thus imperative for prognosis of cancer immunotherapy yet remains challenging. Herein, we report a Tandem-locked NETosis Reporter 1 (TNR1 ) that activates fluorescence signals only in the presence of both neutrophil elastase (NE) and cathepsin G (CTSG) for the specific imaging of NETosis. …”
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Quantitative glucose detection by surface-enhanced Raman spectroscopy using viologens as reporter. by Cheng, Chun Chieh.

“…According to previous studies, glucose has been difficult to detect by SERS due to its poor Raman cross section and its inability to absorb onto metallic surfaces. In this report, the study of indirect glucose detection via SERS is documented. …”
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Investigating cell type specific metabolism using GFP as a reporter protein by Rossi, M, Ratcliffe, R, Kruger, N

“…The fundamental difficulty in applying a reporter protein strategy in a multicellular organism arises from the limited amount of recombinant protein expressed by the cells.…”

Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter. by Victora, G, Schwickert, T, Fooksman, DR, Kamphorst, A, Meyer-Hermann, M, Dustin, M, Nussenzweig, M

Nestin reporter transgene labels multiple central nervous system precursor cells. by Walker, A, Goings, G, Kim, Y, Miller, R, Chenn, A, Szele, F

Rational optimization and imaging in vivo of a genetically encoded optical voltage reporter. by Sjulson, L, Miesenböck, G

“…We evaluate strategies for potential further improvement of hVOS quantitatively and derive theoretical performance limits for optical voltage reporters in general.…”

Technical Advance: Fluorescent reporter reveals insights into eomesodermin biology in cytotoxic lymphocytes. by Paley, M, Gordon, S, Bikoff, E, Robertson, E, Wherry, E, Reiner, S

“…Characterization of reporter activity in NK lineage cells also permitted identification of a novel intermediate of NK cell maturation. …”

Agrobacterium-mediated genetic transformation of Pjalaenopsis using GFP and GUS reporter genes. by Mahmood, Maziah, Chew, Yee Chern