Deconstructing the trypanosome cytoskeleton: from structures to functions via components and complexes. by Portman, N

“…</p> <p>I have used a combined reverse genetics/comparative proteomics approach to identify over 20 novel components of the paraflagellar rod, an essential structure for the mammalian infective form of the parasite. …”

Characterisation and rescue of a recombinant newcastle disease virus strain AF2240-I by Kavitha, Murulitharan

“…The main objectives of this study are to sequence the full genome of NDV strain AF2240-I and perform in-silico characterisation and to generate a reverse genetics system to rescue stably virulent NDV strain AF2240-I. …”
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Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus by Tamires Cruz dos Santos, Mariana Ramos Leandro, Clara Yohana Maia, Patrícia Rangel, Fabiano S. Soares, Ricardo Reis, Lucas Passamani, Vanildo Silveira, Gonçalo Apolinário de Souza Filho

“…Finally, the role of some up-accumulated proteins in the response G. diazotrophicus to cocultivation was analyzed by reverse genetics, using insertion mutants. Results Our results revealed the induction of bacterial growth in response to cocultivation. …”
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Studies of an influenza A virus temperature-sensitive mutant identify a late role for NP in the formation of infectious virions. by Noton, S, Simpson-Holley, M, Medcalf, E, Wise, H, Hutchinson, E, McCauley, J, Digard, P

“…Introduction of this mutation into A/PR/8/34 virus by reverse genetics produced a ts phenotype, confirming the identity of the lesion. …”

Development of a T7 RNA polymerase expressing cell line using lentivirus vectors for the recovery of recombinant Newcastle disease virus by Yeong, Ming Yue, Cheow, Pheik Sheen, Abdullah, Syahril, Song, Adelene Ai Lian, Lei, Janet Rossmann, Tan, Tiong Kit, Yusoff, Khatijah, Chia, Suet Lin

“…BSR T7/5 cells marks an improvement of reverse genetics for the recovery of recombinant Newcastle disease virus (rNDV). …”
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Virulence of newcastle disease virus: what is known so far? by Dortmans Jos CFM, Koch Guus, Rottier Peter JM, Peeters Ben PH

“…This is mainly due to the development of reverse genetics systems which made it possible to genetically modify NDV and to investigate the contribution of individual genes and genome regions to its virulence. …”
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Rescue of influenza A virus from recombinant DNA. by Fodor, E, Devenish, L, Engelhardt, O, Palese, P, Brownlee, G, García-Sastre, A

“…This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.…”

Systems Biology Approaches Reveal Potential Phenotype-Modifier Genes in Neurofibromatosis Type 1 by Thayne Woycinck Kowalski, Larissa Brussa Reis, Tiago Finger Andreis, Patricia Ashton-Prolla, Clévia Rosset

“…Protein–protein interaction (PPI) networks were assembled, and network statistics were obtained by using forward and reverse genetics strategies. We also evaluated the heterogeneous networks comprising the phenotype ontologies selected, gene expression data, and the PPI network. …”
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Genetic analysis : an integrated approach / by 531048 Sanders, Mark Frederick, Bowman, John L. 531049

Development of a recombinant genotype-matched live attenuated vaccine against genotype VII Newcastle disease virus by Bello, Muhammad Bashir

“…Therefore in the present study, reverse genetics technology was applied to genetically attenuate the pathogenicity of a recently isolated virulent Malaysian NDV strain IBS025/13. …”
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Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation. by Bernadette Crescenzo-Chaigne, Cyril Barbezange, Vianney Frigard, Damien Poulain, Sylvie van der Werf

“…Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5' NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication.…”
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Cell type dependent stability and virulence of a recombinant SARS-CoV-2, and engineering of a propagation deficient RNA replicon to analyze virus RNA synthesis by Li Wang, María Guzman, Diego Muñoz-Santos, Jose Manuel Honrubia, Jorge Ripoll-Gomez, Rafael Delgado, Isabel Sola, Luis Enjuanes, Sonia Zuñiga

“…Engineering of reverse genetics systems for newly emerged viruses allows viral genome manipulation, being an essential tool for the study of virus life cycle, virus-host interactions and pathogenesis, as well as for the development of effective antiviral strategies. …”
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Molecular Genetics & Gene Expression / by Cornish, Marquita, author 650295, Carlisle, Kane, author 639827

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Lipid Metabolism in Plants by Hyun Uk Kim

“…The metabolism of fatty acids and triacylglycerols is well studied in most Arabidopsis model plants by forward and reverse genetics methods. However, research on the diverse functions of lipids in plants, including various crops, has yet to be completed. …”
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Attenuation of neurovirulence of chikungunya virus by a single amino acid mutation in viral E2 envelope protein by Huixin Chen, Patchara Phuektes, Li Sze Yeo, Yi Hao Wong, Regina Ching Hua Lee, Bowen Yi, Xinjun Hou, Sen Liu, Yu Cai, Justin Jang Hann Chu

“…Additionally, whole viral genome analysis and reverse genetics were employed to identify genomic virulence factors. …”
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Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana. by Wang, Z, Gu, C, Colby, T, Shindo, T, Balamurugan, R, Waldmann, H, Kaiser, M, van der Hoorn, R

“…Inhibitor studies and reverse genetics led to the discovery that this unusual modification is mediated by a single plant-specific, papain-like protease called RD21. …”

Understanding bat SARS-like coronaviruses for the preparation of future coronavirus outbreaks — Implications for coronavirus vaccine development by Oi-Wing Ng, Yee-Joo Tan

“…The application of reverse genetics further enabled that characterization of these bat CoVs and the prediction of their potential to cause disease in humans. …”
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Live attenuated-nonpathogenic Leishmania and DNA structures as promising vaccine platforms against leishmaniasis: innovations can make waves by Negar Seyed, Tahereh Taheri, Sima Rafati

“…Innovative approaches such as reverse genetics, CRISP/R-Cas9 and antibiotic-free selection are now available to promisingly compensate for intrinsic drawbacks associated with these platforms. …”
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Viral Inhibition of the IFN-Induced JAK/STAT Signalling Pathway: Development of Live Attenuated Vaccines by Mutation of Viral-Encoded IFN-Antagonists by Stephen B. Fleming

“…Moreover, identification of factors encoded by viruses that modulate the JAK/STAT pathway has opened up opportunities to create new anti-viral drugs and rationally attenuated new generation vaccines, particularly for RNA viruses, by reverse genetics.…”
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Bench Research Informed by GWAS Results by Nikolay V. Kondratyev, Margarita V. Alfimova, Arkadiy K. Golov, Vera E. Golimbet

“…The study of such traits presents a challenge for researchers, as the complex genetic architecture of such traits makes it nearly impossible to utilise many of the usual methods of reverse genetics, which often focus on specific genes. …”
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