Applications of Deep Mutational Scanning in Virology by Thomas D. Burton, Nicholas S. Eyre

“…We also briefly discuss the reverse genetics systems which allow for analysis of viral replication cycles, next-generation sequencing technologies and the bioinformatics tools that facilitate this research.…”
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Building a hair: tip growth in Arabidopsis thaliana root hairs. by Carol, R, Dolan, L

“…A role for LRX1, a leucine-rich repeat extensin, in determining the morphology of the cell wall of root hairs has been established using reverse genetics. The new information can be integrated into a general and more advanced view of how these specialized plant cells grow.…”

Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 by Nik Nadia, Nik Mohd Nasir, Tan, Chee Wah, Ong, Kien Chai, Sam, I-Ching, Chan, Yoke Fun

“…The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. …”

An improved method for the rescue of of recombinant Newcastle disease virus by Cheow, Pheik Sheen, Tan, Tiong Kit, Song, Adelene Ai Lian, Yusoff, Khatijah, Chia, Suet Lin

“…Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. …”
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Modulation of sarcoplasmic reticulum calcium release by calsequestrin in cardiac myocytes by SANDOR GYÖRKE, INNA GYÖRKE, DMITRY TERENTYEV, SERGE VIATCHENKO-KARPINSKI, SIMON C WILLIAMS

“…We have studied the function of CASQ2 and the consequences of arrhythmogenic CASQ2 mutations on intracellular Ca signalling using a combination of approaches of reverse genetics and cellular physiology in adult cardiac myocytes. …”
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Applications of Virus-Induced Gene Silencing in Cotton by Yue Tian, Yao Fang, Kaixin Zhang, Zeyang Zhai, Yujie Yang, Meiyu He, Xu Cao

“…Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become an effective tool to investigate gene function in plants. …”
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Development and Use of an Efficient DNA-Based Viral Gene Silencing Vector for Soybean by Chunquan Zhang, Chunling Yang, Steven A. Whitham, John H. Hill

“…Virus-induced gene silencing (VIGS) is increasingly being used as a reverse genetics tool to study functions of specific plant genes. …”
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CRISPR/Cas9 Mediated KO Studies Identified Essential Genes for Fertilization and Oocyte Activation by Masahito Ikawa

“…CRISPR/Cas9 system has opened a new era for reverse genetics. Taking advantage of the CRISPR/Cas9-mediated approaches, our laboratory has knocked out 421 male reproductive organ-specific genes and analysed their phenotypes in vivo. …”
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Measles Virus as an Oncolytic Immunotherapy by Christine E. Engeland, Guy Ungerechts

“…The versatile MeV reverse genetics systems allows for engineering of advanced targeted, armed, and shielded oncolytic viral vectors. …”
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FUNCTIONAL CHARACTERIZATION OF SPERMINE FAMILY TRANSPORTER caf5+ IN Schizosaccharomyces pombe (Lindner) by Aslıhan Örs Gevrekci

“…This study aims to understand the contribution of caf5+ on different processes previously associated with polyamines, by reverse genetics. Deletion mutants of caf5+, which are viable in normal conditions, were scanned for multiple cellular processes. …”
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BIOTECHNOLOGICAL RESEARCH IN THE CREATION AND PRODUCTION OF ANTIRABIC VACCINES by Krasnopolsky Yu. М., Pylypenko D. М.

“…The advantages of different vaccine types are considered: live attenuated, peptide, liposomal, RNA vaccines, vaccines based on viral vectors, transgenic plants and reverse genetics methods. Conclusions. The development of biotechnology, immunology and virology makes it possible to improve constantly vaccine preparations, including those against rabies, increasing their effectiveness and safety.…”
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Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections by Ellison, Aubrey J, Dempwolff, Felix, Kearns, Daniel B, Raines, Ronald T

“…To identify a molecular target, we used reverse genetics to "knock-in"the GAS genes that encode two proteins with collagen-like domains, Scl1 and Scl2, into B. subtilis. …”
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Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion. by Halldorsson, S, Behrens, A, Harlos, K, Huiskonen, J, Elliott, R, Crispin, M, Brennan, B, Bowden, T

“…By comparison of SFTSV Gc with that of the prefusion structure of the related Rift Valley fever virus, we show that these changes involve a dimer-to-trimer transition and refolding of the protein. Reverse genetics and rescue of site- directed histidine mutants enabled localization of histidines likely to be important for triggering this pH-dependent process. …”

Stage-specific depletion of myosin A supports an essential role in motility of malarial ookinetes. by Siden-Kiamos, I, Ganter, M, Kunze, A, Hliscs, M, Steinbüchel, M, Mendoza, J, Sinden, R, Louis, C, Matuschewski, K

“…Functional analysis of Plasmodium genes by classical reverse genetics is currently limited to mutants that are viable during erythrocytic schizogony, the pathogenic phase of the malaria parasite where transfection is performed. …”

The cell biology of parasitism in Trypanosoma brucei: insights and drug targets from genomic approaches? by Gull, K

“…The review also focuses on the likely impact of the genome project and reverse genetics in providing greater insight to these cellular processes and how, if coordinated with some alan by scientists and funding agencies, this may provide novel targets for future drug development.…”

The structural mechanics of cell division in Trypanosoma brucei. by Vaughan, S, Gull, K

“…This structural precision is aiding progress in understanding these processes as we apply the excellent reverse genetics and post-genomic technologies available in this system. …”

Vaccines for contagious diseases by Oi Leng, Irene Liew, Toh, Hwee Ting, Mohammed, Elizah, Mohd Fauzi, Noor Akhmazillah

“…With the improvements made in biotechnology, the production and effectiveness of vaccines also experience an advancement from the commonly applied recombinant DNA technology to the reverse genetics’ techniques and newly DNA vaccines which is still not common in the applications for humans. …”
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Vaccines for contagious diseases by Liew Oi Leng, Irene, Toh, Hwee Ting, Mohammed, Elizah, Mohd Fauzi, Noor Akhmazillah

“…With the improvements made in biotechnology, the production and effectiveness of vaccines also experience an advancement from the commonly applied recombinant DNA technology to the reverse genetics’ techniques and newly DNA vaccines which is still not common in the applications for humans. …”
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Calcineurin Is an Antagonist to PKA Protein Phosphorylation Required for Postmating Filamentation and Virulence, While PP2A Is Required for Viability in Ustilago maydis by John D. Egan, María D. García-Pedrajas, David L. Andrews, Scott E. Gold

“…The roles of two protein phosphatases that potentially act antagonistically to PKA were assessed. A reverse genetics approach to mutate the catalytic subunits of calcineurin (CN, protein phosphatase [PP]2B) and PP2A in U. maydis was employed. …”
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The Cellular and Chemical Biology of Endocytic Trafficking and Intracellular Delivery—The GL–Lect Hypothesis by Ludger Johannes

“…Two of the most abundant fundamental fabrics of membranes—lipids and complex sugars—are produced through elaborate chains of biosynthetic enzymes, which makes it difficult to study them by conventional reverse genetics. This review illustrates how organic synthesis provides access to uncharted areas of membrane glycobiology research and its application to biomedicine. …”
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