TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding by Taheri, Sima, Abdullah, Thohirah Lee, Jain, Shri, Sahebi, Mahbod, Azizi, Parisa

“…With the assistance of reverse genetic techniques, sequence variation information can be linked to traits to investigate gene function. …”
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Diagnostic and vaccination approaches for Newcastle disease virus in poultry: the current and emerging perspectives by Bello, Muhammad Bashir, Yusoff, Khatijah, Ideris, Aini, Bejo, Mohd Hair, Peeters, Ben P. H., Omar, Abdul Rahman

“…Among the recently evolving technologies for the development of genotype-matched vaccines, reverse genetics-based live attenuated vaccines obviously appeared to be the most promising candidates. …”
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Characterization and functional analysis of AhGPAT9 gene involved in lipid synthesis in peanut (Arachis hypogaea L.) by Yue Shen, Yi Shen, Yonghui Liu, Yang Bai, Man Liang, Xuyao Zhang, Zhide Chen

“…GPAT enzymes (glycerol-3-phosphate 1-O-acyltransferase, EC 2.3.1.15) catalyze the initial and rate-limiting step of plant glycerolipid biosynthesis for membrane homeostasis and lipid accumulation, yet little research has been done on peanuts. By reverse genetics and bioinformatics analyses, we have characterized an AhGPAT9 isozyme, of which the homologous product is isolated from cultivated peanut. …”
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Genetic stability of the HA, NA, and NS genes of the recombinant vector virus FluNS1-124-Omp16 (H5N1) expressing the brucellar gene by S. O. Sadikaliyeva, K. T. Sultankulova, K. A. Shorayeva, V. M. Strochkov, O. V. Chervyakova, V. L. Zaitsev, K. K. Tabynov, N. T. Sandybayev, A. R. Sansyzbay, A. Yu. Egorov

“…The recombinant strain Flu-NS1-124-Omp16 (H5N1) of the influenza virus expressing the brucellar Omp16 gene was constructed on the basis of the technology of reverse genetics for the purpose of developing vector antibrucellosis vaccine. …”
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Protein-RNA linkage and posttranslational modifications of feline calicivirus and murine norovirus VPg proteins by Allan Olspert, Myra Hosmillo, Yasmin Chaudhry, Lauri Peil, Erkki Truve, Ian Goodfellow

“…However, a number of caliciviruses replicate efficiently in cell culture and have robust reverse genetics systems available, most notably feline calicivirus (FCV) and murine norovirus (MNV). …”
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The Early Endocytosis Gene <i>PAL1</i> Contributes to Stress Tolerance and Hyphal Formation in <i>Candida albicans</i> by Miranda Yu, Dakota Ma, Susan Eszterhas, Christiane Rollenhagen, Samuel A. Lee

“…In this study, we examined the functions of the early endocytosis gene <i>PAL1</i> using a reverse genetics approach based on CRISPR-Cas9-mediated gene deletion. …”
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Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the <i>FAD2</i> and <i>FATB</i> Multigene in... by Won-Nyeong Kim, Hye-Jeong Kim, Young-Soo Chung, Hyun-Uk Kim

“…CRISPR/Cas9 is a commonly used technique in reverse-genetics research to knock out a gene of interest. …”
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Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus by Giovanni Messina, Yuri Prozzillo, Francesca Delle Monache, Maria Virginia Santopietro, Patrizio Dimitri

“…Results We have combined cell biology, reverse genetics, and biochemical approaches to study the subcellular distribution of a number of subunits belonging to the SRCAP and p400/Tip60 complexes and assess their involvement during cell division progression in HeLa cells. …”
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Investigation of Antifungal Mechanisms of Thymol in the Human Fungal Pathogen, <i>Cryptococcus neoformans</i> by Kwang-Woo Jung, Moon-Soo Chung, Hyoung-Woo Bai, Byung-Yeoup Chung, Sungbeom Lee

“…Although plants produce a diversity of chemicals to protect themselves from pathogens, the molecular targets and modes of action of these plant-derived chemicals have not been well characterized. Using a reverse genetics approach, the present study revealed that thymol, a monoterpene alcohol from <i>Thymus vulgaris</i> L., (Lamiaceae), exhibits antifungal activity against <i>Cryptococcus neoformans</i> by regulating multiple signaling pathways including calcineurin, unfolded protein response, and HOG (high-osmolarity glycerol) MAPK (mitogen-activated protein kinase) pathways. …”
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Suramin action in African trypanosomes involves a RuvB-like DNA helicase by Anna Albisetti, Silvan Hälg, Martin Zoltner, Pascal Mäser, Natalie Wiedemar

“…The introduction of this missense mutation, by reverse genetics, into drug-sensitive trypanosomes significantly decreased their sensitivity to suramin. …”
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Adaptation of the H7N2 Feline Influenza Virus to Human Respiratory Cell Culture by Wataru Sekine, Akiko Takenaka-Uema, Haruhiko Kamiki, Hiroho Ishida, Hiromichi Matsugo, Shin Murakami, Taisuke Horimoto

“…In this study, to gain insight into the molecular basis of the transmission of the feline virus to humans, we selected mutant viruses with enhanced growth in human respiratory A549 cells via successive passages of the virus and found almost all mutations to be in the envelope glycoproteins, such as hemagglutinin (HA) and neuraminidase (NA). The reverse genetics approach revealed that the HA mutations, HA1-H16Q, HA2-I47T, or HA2-Y119H, in the stalk region can lead to a high growth of mutant viruses in A549 cells, possibly by changing the pH threshold for membrane fusion. …”
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Loss of IκB kinase β promotes myofibroblast transformation and senescence through activation of the ROS-TGFβ autocrine loop by Liang Chen, Zhimin Peng, Qinghang Meng, Maureen Mongan, Jingcai Wang, Maureen Sartor, Jing Chen, Liang Niu, Mario Medvedovic, Winston Kao, Ying Xia

“…ABSTRACT Using forward and reverse genetics and global gene expression analyses, we explored the crosstalk between the IκB kinase β (IKKβ) and the transforming growth factor β (TGFβ) signaling pathways. …”
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A Recombinant Genotype I Japanese Encephalitis Virus Expressing a <i>Gaussia</i> Luciferase Gene for Antiviral Drug Screening Assay and Neutralizing Antibodies Detection by Chenxi Li, Xuan Chen, Jingbo Hu, Daoyuan Jiang, Demin Cai, Yanhua Li

“…In this study, we established a reverse genetics system for the GI JEV strain (YZ-1) using a homologous recombination strategy. …”
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Complexity of immunobiology of tumor necrosis factor and novel anti-TNF therapy by S. А. Nedospasov

“…Our studies in mice based on the technologies of reverse genetics and experimental disease models, revealed a paradoxical feature of TNF: some cellular sources of this cytokine (such as myeloid cells) promoted diseases, while other cell types (such as T lymphocytes) produced a protective form of the same cytokine. …”
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Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens by Sohini Dey, Madhan Mohan Chellappa, Dinesh C. Pathak, Satish Gaikwad, Kalpana Yadav, Saravanan Ramakrishnan, Vikram N. Vakharia

“…Here, we demonstrate that a bivalent NDV-IBDV vaccine candidate generated by reverse genetics method is safe, efficacious and cost-effective, which will greatly aid the poultry industry in developing countries.…”
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Analysis of porcine bone marrow-derived macrophage cytokine responses to infection with PRRSV-1 strains of differing virulence by Ben Jackson, Tiphany Chrun, Kay Childs, Nanchaya Wanasen, Jean-Pierre Frossard, Simon P. Graham, Julian Seago

“…To address this knowledge gap, we sought to develop a reverse genetics and ex vivo model system, to attempt to identify correlates of PRRSV-1 virulence and attenuation in vitro. …”
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Increased Polymerase Activity of Zoonotic H7N9 Allows Partial Escape from MxA by Philipp P. Petric, Jacqueline King, Laura Graf, Anne Pohlmann, Martin Beer, Martin Schwemmle

“…In MxA^tg/−Rag2^−/−, but not in Rag2^−/− mice, the well-described mammalian adaptation E627K in the viral polymerase subunit PB2 was acquired, but no variants with MxA escape mutations in NP were detected. Utilizing reverse genetics, we could show that acquisition of PB2 E627K allowed partial evasion from MxA restriction in MxA^tg/tg mice. …”
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Non-structural protein NS3/NS3a is required for propagation of bluetongue virus in Culicoides sonorensis by Femke Feenstra, Barbara S. Drolet, Jan Boonstra, Piet A. van Rijn

“…Virus strains were generated using reverse genetics and their growth was examined in vitro. …”
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Detection of genomic deletions in rice using oligonucleotide microarrays by Bordeos Alicia, Sugiyama Nobuko, Diaz M Genaleen, Bai Jianfa, Mauleon Ramil, Hess Ann, Bruce Myron, Wang Guo-Liang, Leung Hei, Leach Jan E

“…Our study focuses not only on the utility of this method for forward genetics, but also its potential as a reverse genetics tool through accumulation of hybridization data for a collection of deletion mutants harboring multiple genetic lesions.…”
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Enthralling genetic regulatory mechanisms meddling insecticide resistance development in insects: role of transcriptional and post-transcriptional events by Chandramohan Muthu Lakshmi Bavithra, Marimuthu Murugan, Shanmugasundaram Pavithran, Kathirvel Naveena

“…An overview of molecular mechanisms underlying these biological rhythms allows for developing alternative pest control methods to focus on insect vulnerabilities, employing reverse genetics approaches like RNA interference (RNAi) technology to silence particular resistance-related genes for sustained insect management.…”
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