Natural variants of AtHKT1 enhance Na+ accumulation in two wild populations of Arabidopsis. by Ana Rus, Ivan Baxter, Balasubramaniam Muthukumar, Jeff Gustin, Brett Lahner, Elena Yakubova, David E Salt

“…Here, we report on the coupling of high-throughput elemental profiling of shoot tissue from various Arabidopsis accessions with DNA microarray-based bulk segregant analysis and reverse genetics, for the rapid identification of genes from wild populations of Arabidopsis that are involved in regulating how plants acquire and accumulate Na(+) from the soil. …”
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MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing by Madhura M. Yapa, Paymon Doroodian, Paymon Doroodian, Zhenyu Gao, Zhenyu Gao, Peifeng Yu, Peifeng Yu, Zhihua Hua, Zhihua Hua

“…In addition, our newly developed iCRISPRi approach provided a novel genetic tool for quantitative reverse genetics studies on hub genes in plants.…”
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Multiplexed CRISPR/Cas9 genome editing increases the efficacy of homologous-dependent repair of donor sequences in mammalian cells by Ezio T. Fok, Clement B. Penny, Musa M. Mhlanga, Marc S. Weinberg

“…Efficient and robust genome editing tools and strategies allow for specific and exact genetic changes to be captured in model systems, thereby accelerating both forward and reverse genetics studies. The development of CRISPR/Cas9 as a facile designer nuclease toolset has allowed for defined genetic modifications to be efficiently made through homology-directed repair of targeted DNA double-stranded breaks (DSBs) using exogenous repair templates. …”
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Development of a Recombinant Pichinde Virus-Vectored Vaccine against Turkey Arthritis Reovirus and Its Immunological Response Characterization in Vaccinated Animals by Pawan Kumar, Tamer A. Sharafeldin, Rahul Kumar, Qinfeng Huang, Yuying Liang, Sagar M. Goyal, Robert E. Porter, Hinh Ly, Sunil K. Mor

“…Here, we describe the use of reverse genetics technology to generate a recombinant Pichinde virus (PICV) that expresses the Sigma C and/or Sigma B proteins of TARV as antigens. …”
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AddaVax-Adjuvanted H5N8 Inactivated Vaccine Induces Robust Humoral Immune Response against Different Clades of H5 Viruses by Feixia Gao, Xueying Liu, Yudong Dang, Peng Duan, Wenting Xu, Xin Zhang, Shilei Wang, Jian Luo, Xiuling Li

“…In the present study, a vaccine candidate virus based on newly human-infected A/Astrakhan/3212/2020 H5N8 virus was constructed by reverse genetics (RG) technology. The immunogenicity of H5N8 whole virion inactivated vaccine was evaluated by various doses of vaccine antigen formulated with squalene-based adjuvant (AddaVax), aluminum hydroxide (Al(OH)₃) or without adjuvant in mice. …”
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A New Role for Plastid Thioredoxins in Seed Physiology in Relation to Hormone Regulation by Guillaume Née, Gilles Châtel-Innocenti, Patrice Meimoun, Juliette Leymarie, Françoise Montrichard, Pascale Satour, Christophe Bailly, Emmanuelle Issakidis-Bourguet

“…We hypothesized that Trx y1 and Trx y2 would play an important role in seed physiology as antioxidants. Using reverse genetics, we found important changes in the corresponding Arabidopsis mutant seeds. …”
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Anilinopyrimidine Resistance in Botrytis cinerea Is Linked to Mitochondrial Function by Andreas Mosbach, Dominique Edel, Andrew D. Farmer, Andrew D. Farmer, Stephanie Widdison, Thierry Barchietto, Robert A. Dietrich, Andy Corran, Gabriel Scalliet

“…In order to explore the widest range of possible resistance mechanisms, AP-resistant B. cinerea UV laboratory mutants were generated and the mutations conferring resistance were determined by combining whole-genome sequencing and reverse genetics. Genetic mapping from a cross between a resistant field isolate and a sensitive reference isolate was used in parallel and led to the identification of an additional molecular determinant not found from the characterized UV mutant collection. …”
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The Cellular Protein CAD is Recruited into Ebola Virus Inclusion Bodies by the Nucleoprotein NP to Facilitate Genome Replication and Transcription by Janine Brandt, Lisa Wendt, Bianca S. Bodmer, Thomas C. Mettenleiter, Thomas Hoenen

“…To this end, we used siRNA knockdowns in combination with various reverse genetics-based life cycle modelling systems and additionally performed co-immunoprecipitation and co-immunofluorescence assays to investigate the influence of CAD on individual aspects of the EBOV life cycle and to characterize the interactions of CAD with viral proteins. …”
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Competitive Fitness of Influenza B Viruses Possessing E119A and H274Y Neuraminidase Inhibitor Resistance-Associated Substitutions in Ferrets. by Philippe Noriel Q Pascua, Bindumadhav M Marathe, Andrew J Burnham, Peter Vogel, Richard J Webby, Robert G Webster, Elena A Govorkova

“…We evaluated in ferrets the pathogenicity and relative fitness of reverse genetics (rg)-generated influenza B/Yamanashi/166/1998-like viruses containing E119A or H274Y NA substitutions (N2 numbering). …”
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Proline to Threonine Mutation at Position 162 of NS5B of Classical Swine Fever Virus Vaccine C Strain Promoted Genome Replication and Infectious Virus Production by Facilitating In... by Huining Pang, Ling Li, Hongru Liu, Zishu Pan

“…We generated P162T mutants of two chimeras using the reverse genetics system, separately. The single P162T mutation in NS5B of vC/SM3′UTR or vC/b3′UTR played a key role in increased viral genome replication and infectious virus production. …”
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Applications of genome editing for disease modeling in mice by Platt, Randall Jeffrey

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Site-directed mutagenesis of newcastle disease VIRUS V protein in virus pathogenicity by Tham, May Ling

“…The NDV antigenome plasmids containing the mutated V genes were co-transfected with helper plasmids (plasmids containing NP, P, and L genes) into BSR T7/5 cells to produce the recombinant NDV (rNDV) by reverse genetics. The virus was then rescued and propagated in embryonated chicken eggs. …”
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Site-directed mutagenesis of newcastle disease VIRUS V protein in virus pathogenicity by Tham, May Ling

“…The NDV antigenome plasmids containing the mutated V genes were co-transfected with helper plasmids (plasmids containing NP, P, and L genes) into BSR T7/5 cells to produce the recombinant NDV (rNDV) by reverse genetics. The virus was then rescued and propagated in embryonated chicken eggs. …”
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NS1 Protein N-Linked Glycosylation Site Affects the Virulence and Pathogenesis of Dengue Virus by Enyue Fang, Miao Li, Xiaohui Liu, Kongxin Hu, Lijuan Liu, Zelun Zhang, Xingxing Li, Qinhua Peng, Yuhua Li

“…Recently, site-directed mutation of the flavivirus genome using reverse genetics techniques has been used for the rapid development of attenuated vaccines. …”
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Updating the National Antigen Bank in Korea: Protective Efficacy of Synthetic Vaccine Candidates against H5Nx Highly Pathogenic Avian Influenza Viruses Belonging to Clades 2.3.2.1... by Yong-Myung Kang, Hyun-Kyu Cho, Sung-Jun An, Hyun-Jun Kim, Youn-Jeong Lee, Hyun-Mi Kang

“…To update the vaccine strains in the antigen bank, we used reverse genetics technology to develop two vaccine candidates against avian influenza strains belonging to clades 2.3.2.1d and 2.3.4.4h, and then evaluated their immunogenicity and protective efficacy in SPF chickens challenged with H5 viruses. …”
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The nonstructural p17 protein of a fusogenic bat-borne reovirus regulates viral replication in virus species- and host-specific manners by Ryotaro Nouda, Takahiro Kawagishi, Yuta Kanai, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi

“…Here, various p17 mutant viruses including p17-deficient viruses were generated by a reverse genetics system for NBV. The results demonstrated that p17 is not essential for viral replication and does not play an important role in viral pathogenesis. …”
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MiRNA Targeted NP Genome of Live Attenuated Influenza Vaccines Provide Cross-Protection against a Lethal Influenza Virus Infection by Feixia Gao, Tianhan Yang, Xueying Liu, Feifei Xiong, Jian Luo, Yinglei Yi, Jiangfeng Fan, Ze Chen, Wen-Song Tan

“…In this study, the nucleoprotein (NP) genome segment of the influenza virus was inserted by different perfect miRNA-192-5p target sites, and the virus was rescued by standard reverse genetics method, so as to verify the virulence and protective efficacy of live attenuated vaccine in cells and mice. …”
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A clinically attenuated double-mutant of porcine reproductive and respiratory syndrome virus-2 that does not prompt overexpression of proinflammatory cytokines during co-infection... by Chia-Ming Su, Jineui Kim, Junyu Tang, Yu Fan Hung, Federico A Zuckermann, Robert Husmann, Patrick Roady, Jiyoun Kim, Young-Min Lee, Dongwan Yoo

“…In the present study, we generated a double-mutant PRRSV-2 that contained the L126A mutation in the nsp1β gene and the NLS mutation (ΔNLS) in the N gene using reverse genetics. The immunological phenotype of this mutant PRRSV-2 was examined in porcine alveolar macrophages (PAMs) in vitro and in young pigs in vivo. …”
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Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens by Deep Prakash Saikia, Kalpana Yadav, Dinesh C. Pathak, Narayan Ramamurthy, Ajai Lawrence D’Silva, Asok Kumar Marriappan, Saravanan Ramakrishnan, Vikram N. Vakharia, Madhan Mohan Chellappa, Sohini Dey

“…In this seminal work, a recombinant NDV strain R2B expressing the sigma C (σC) gene of ARV (rNDV-R2B-σC) was generated by reverse genetics, characterized in vitro and tested as a bivalent vaccine candidate in chickens. …”
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Porcine Reproductive and Respiratory Syndrome Virus Modulates the Switch of Macrophage Polarization from M1 to M2 by Upregulating MoDC-Released sCD83 by Xingyu Gong, Tianyi Ma, Qiaoya Zhang, Yanhong Wang, Chengchuang Song, Min Lai, Chunlei Zhang, Xingtang Fang, Xi Chen

“…Neutralization of sCD83 removes the inhibitory effects of PRRSV on PAMs. Using reverse genetics, we generated recombinant PRRSVs with mutations in N protein, nsp1α, and nsp10 (knockout sCD83-concerned key amino acid site). …”
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