Immunogenicity and Therapeutic Efficacy of a Sendai-Virus-Vectored HSV-2 Vaccine in Mouse and Guinea Pig Models by Xiuxiu Ren, Wenhao Su, Shishi Li, Tingting Zhao, Qiufang Huang, Yinan Wang, Xiaojie Wang, Xiaohuan Zhang, Jiangbo Wei

“…Methods: We constructed two recombinant vectors, one encoding HSV-2 glycoprotein D (gD, SeV-dF/HSV-2-gD) and one encoding HSV-2-infected cell protein 27 (ICP27, SeV-dF/HSV-2-ICP27), based on a replication-defective Sendai virus through reverse genetics, collectively comprising a combinatorial HSV-2 therapeutic vaccine candidate. …”
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Domesticating Vigna stipulacea: Chromosome-Level genome assembly reveals VsPSAT1 as a candidate gene decreasing hard-seededness by Yu Takahashi, Hiroaki Sakai, Hirotaka Ariga, Shota Teramoto, Takashi L. Shimada, Takashi L. Shimada, Heesoo Eun, Chiaki Muto, Ken Naito, Norihiko Tomooka

“…Given that there are multiple stress-tolerant wild legume species, it is important to establish efficient domestication processes using reverse genetics and identify the genes responsible for domestication traits. …”
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In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts by Carolyn A. Owen, Romy Moukarzel, Xiao Huang, Mona A. Kassem, Eleonora Eliasco, Miguel A. Aranda, Robert H. A. Coutts, Ioannis C. Livieratos

“…The clone T3CM8Δ will facilitate reverse genetics studies of CYSDV gene function and RNA replication determinants.…”
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Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam by Ogawa, Haruko, Imai, Kunitoshi, Bui, Vuong Nghia, Trinh, Dai Quang, Nguyen, Tham Hong Thi, Pham, Nga Thi, Truong, Duc Anh, Bui, Anh Ngoc, Nguyen, Khong Viet, Runstadler, Jonathan

“…Those mutations may be related to the escape of virus from antibody binding and the infection of poultry, interpretations which may be confirmed through a reverse genetics approach. The virus also carried an amino acid substitution in the M2, which conferred a reduced susceptibility to amantadine, but no neuraminidase inhibitor resistance markers were found in the viral NA gene. …”
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Developmental transitions of germ cell lineage of the mouse by Baltus, Andrew Edmund

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A Single Base-Pair Change in 2009 H1N1 Hemagglutinin Increases Human Receptor Affinity and Leads to Efficient Airborne Viral Transmission in Ferrets by Jayaraman, Akila, Pappas, Claudia, Raman, Rahul, Belser, Jessica A., Viswanathan, Karthik, Tumpey, Terrence M., Sasisekharan, Ram, Shriver, Zachary H.

“…Moreover, a 2009 H1N1 virus carrying this mutation in the RBS (generated using reverse genetics) transmits efficiently in ferrets by respiratory droplets thereby reestablishing our previously observed correlation between human receptor-binding affinity and transmission efficiency. …”
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The biology of kinetoplastid parasites: insights and challenges from genomics and post-genomics. by Gull, K

“…This is an important fact, since in general, it means that we have only recently started to analyse the phenotypes of mutants produced in the context of reverse genetics. In the following, I will argue that this lack of investment in the analysis of mutant phenotype is just one of the challenges that will need to be met if we are to gain the expected added value from the parasite genome projects. …”

Amino Acid Substitutions in Positions 385 and 393 of the Hydrophobic Region of VP4 May Be Associated with Rotavirus Attenuation and Cell Culture Adaptation by Yusheng Guo, David E. Wentworth, Karla M. Stucker, Rebecca A. Halpin, Ham Ching Lam, Douglas Marthaler, Linda J. Saif, Anastasia N. Vlasova

“…Collectively, our data suggest that the VP4 hydrophobic region may play an important role in RV attenuation and aa385 and aa393 may represent potential targets for RV vaccine development using reverse genetics and site-specific mutagenesis.…”
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Attenuation and efficacy of human parainfluenza virus type 1 (HPIV1) vaccine candidates containing stabilized mutations in the P/C and L genes by Skiadopoulos Mario H, Collins Peter L, Surman Sonja R, Castaño Adam, Bartlett Emmalene J, Murphy Brian R

“…<p>Abstract</p> <p>Background</p> <p>Two recombinant, live attenuated human parainfluenza virus type 1 (rHPIV1) mutant viruses have been developed, using a reverse genetics system, for evaluation as potential intranasal vaccine candidates. …”
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A chimeric influenza virus vaccine expressing fusion protein epitopes induces protection from human metapneumovirus challenge in mice by Tian Chongyu, Tian Chongyu, Lei Guanglin, Sun Fang, Deng Zhuoya, Yang Hao, Li Cong, Li Xinyu, He Wei, Tan Lingyun, Niu Yan, Yang Penghui, Yang Penghui, Yang Penghui

“…In this study, we constructed a novel recombinant influenza virus carrying partial HMPV fusion protein (HMPV-F), termed rFLU-HMPV/F-NS, utilizing reverse genetics, which contained (HMPV-F) in the background of NS segments of influenza virus A/PuertoRico/8/34(PR8). …”
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Research Progress on Molecular Mechanism of Salt Tolerance in Rice by Sirong CHEN, Chen LI, Bingrui SUN

“…Nevertheless, many cloned genes related to salt tolerance are difficult to be applied in rice breeding currently as most of them are obtained through reverse genetics methods and exhibit salt tolerance only under overexpression conditions, or these genes are recessive genes. …”
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Peroxisome Proliferator FpPEX11 Is Involved in the Development and Pathogenicity in <i>Fusarium pseudograminearum</i> by Mingyu Wang, Hao Xu, Chunjie Liu, Yilin Tao, Xiaofeng Wang, Yuancun Liang, Li Zhang, Jinfeng Yu

“…The functions of FpPEX11a, FpPEX11b, and FpPEX11c in <i>F. pseudograminearum</i> were studied using reverse genetics, and the results showed that FpPEX11a and FpPEX11b are involved in the regulation of vegetative growth and asexual reproduction. …”
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Changes in the Hemagglutinin and Internal Gene Segments Were Needed for Human Seasonal H3 Influenza A Virus to Efficiently Infect and Replicate in Swine by Daniela S. Rajao, Eugenio J. Abente, Joshua D. Powell, Marcus J. Bolton, Phillip C. Gauger, Bailey Arruda, Tavis K. Anderson, Troy C. Sutton, Daniel R. Perez, Amy L. Vincent Baker

“…For this purpose, reassortant viruses were generated by reverse genetics (rg) swapping the surface genes (hemagglutinin-HA and neuraminidase-NA) and internal gene segment backbones between a human-like H3N1 isolated from swine and a seasonal human H3N2 virus with common HA ancestry. …”
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Establishment and application of a root wounding–immersion method for efficient virus-induced gene silencing in plants by Xinyun Li, Na Tao, Bin Xu, Junqiang Xu, Zhengan Yang, Caiqian Jiang, Ying Zhou, Minghua Deng, Junheng Lv, Kai Zhao

“…In the post-genomic era, virus-induced gene silencing (VIGS) has played an important role in research on reverse genetics in plants. Commonly used Agrobacterium-mediated VIGS inoculation methods include stem scratching, leaf infiltration, use of agrodrench, and air-brush spraying. …”
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MucR Is Required for Transcriptional Activation of Conserved Ion Transporters to Support Nitrogen Fixation of Sinorhizobium fredii in Soybean Nodules by Jian Jiao, Li Juan Wu, Biliang Zhang, Yue Hu, Yan Li, Xing Xing Zhang, Hui Juan Guo, Li Xue Liu, Wen Xin Chen, Ziding Zhang, Chang Fu Tian

“…MucR1 is required for active expression of transporters for phosphate, zinc, and elements essential for nitrogenase activity (iron, molybdenum, and sulfur) in nodules but is dispensable for transcription of key genes (nif/fix) involved in nitrogen fixation. Further reverse genetics suggests that S. fredii uses high-affinity transporters to meet the demand for zinc and phosphate within nodules. …”
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Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus by Lingcai Zhao, Huizhi Xia, Jingjin Huang, Yiqing Zheng, Chang Liu, Juan Su, Jihui Ping

“…First, we generated reassortant viruses via reverse genetics to analyze the segment compatibility and gene interchangeability between IAVs and IDVs. …”
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Rotavirus capping enzyme VP3 inhibits interferon expression by inducing MAVS degradation during viral replication by Jin Dai, Chantal A. Agbemabiese, Ashley N. Griffin, John T. Patton

“…In this study, we have used reverse genetics to generate recombinant rotaviruses expressing compromised forms of VP3 or NSP1, or both, to explore the function of these viral proteins in the degradation of MAVS and IRF3. …”
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Construction and Characterization of Severe Fever with Thrombocytopenia Syndrome Virus with a Fluorescent Reporter for Antiviral Drug Screening by Xiao Wang, Mingyue Xu, Huanhuan Ke, Longda Ma, Liushuai Li, Jiang Li, Fei Deng, Manli Wang, Zhihong Hu, Jia Liu

“…First, we developed a reverse genetics system based on the SFTSV HBMC5 strain. …”
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Structural Requirements in the Hemagglutinin Cleavage Site-Coding RNA Region for the Generation of Highly Pathogenic Avian Influenza Virus by Yurie Kida, Kosuke Okuya, Takeshi Saito, Junya Yamagishi, Aiko Ohnuma, Takanari Hattori, Hiroko Miyamoto, Rashid Manzoor, Reiko Yoshida, Naganori Nao, Masahiro Kajihara, Tokiko Watanabe, Ayato Takada

“…In virus particles generated by using reverse genetics, nucleotide insertions that created additional codons for basic amino acids were found in the RNA region encoding the cleavage site of an H5 HA gene but not in the mutated RNA region. …”
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Generation of a high yield vaccine backbone for influenza B virus in embryonated chicken eggs by Sadaf Aslam, Madhusudan Rajendran, Divya Kriti, Andrew Kurland, Jeffrey Johnson, Harm van Bakel, Florian Krammer, Adolfo García-Sastre, Juan Ayllon

“…We then generated reverse genetics systems for the three clones that performed best in growth curves. …”
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