Molecular characteristics and pathogenicity of infectious cDNA clones of the Chinese isolates of pea enation mosaic virus 1 and pea enation mosaic virus 2 by Xiaojiao Chen, Min Xie, Honglin Chen, Yue Zhang, Kehua Li, Pingxiu Lan, Taiyun Wei, Fan Li

“…The current approach will facilitate determination of the synergistic relationship between PEMV-1 and PEMV-2 and their interactions with the host plant by reverse genetic analysis.…”
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A recombinant rabies virus chimera expressing the DC-targeting molecular MAB2560 shows enhanced vaccine immunogenicity through activation of dendritic cells by Zhiyuan Gong, Pei Huang, Hongli Jin, Yujie Bai, Hailun Li, Meichen Qian, Jingxuan Sun, Cuicui Jiao, Mengyao Zhang, Yuanyuan Li, Haili Zhang, Hualei Wang

“…<h4>Methodology/Principal findings</h4> In this study, we constructed a recombinant virus, rCVS11-MAB2560, based on the reverse genetic system of the RABV CVS11 strain. The MAB2560 protein (a DC-targeting molecular) was chimeric expressed on the surface of the viral particles to help target and activate the DCs when this virus was used as inactivated vaccine. …”
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In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions by Mohamed A. Attia, Cassandra E. Nelson, Wendy A. Offen, Namrata Jain, Gideon J. Davies, Jeffrey G. Gardner, Harry Brumer

“…Strikingly, exhaustive reverse-genetic analysis of all three GH5_4 members and a previously biochemically characterized GH74 member failed to reveal a growth defect, thereby indicating functional compensation in vivo, both among members of this cohort and by other, yet unidentified, xyloglucanases in C. japonicus. …”
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Functional characterization of MATE gene family under abiotic stresses and melatonin-mediated tolerance in dragon fruit (Selenicereus undatus L.) by Darya Khan, Liu Hui, Aamir Ali Khokhar, Muhammad Azhar Hussain, Wei Lv, Qamar U Zaman, Hua-Feng Wang

“…Furthermore, our results provide the foundation for development of stress-tolerant genotypes under various climate scenarios through forward and reverse genetic breeding programs.…”
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The Immunoprotection of <i>OmpH</i> Gene Deletion Mutation of <i>Pasteurella multocida</i> on Hemorrhagic Sepsis in Qinghai Yak by Jianlei Jia, Meng Zhao, Kairu Ma, Hongjian Zhang, Linsheng Gui, Huzhi Sun, Huiying Ren, Tamaki Okabayashi, Jing Zhao

“…The mutant strain was generated through the reverse genetic operation system of pathogens and proteomics technology. …”
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Isolation and characterization of soil chitinolytic bacteria by Ng, Wui Ming

“…However, amplicon of around 400bp for family 18 chitinase gene was only successfully generated from the genome of BRI 1, BRI 8, BRI 13 and BRI 36. A reverse genetic is proposed to solve the problem encountered for BRI 2. …”
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The Arabidopsis T‐DNA mutant SALK_008491 carries a 14‐kb deletion on chromosome 3 that provides rare insights into the plant response to dynamic light stress by Laura S. Lopez, Carsten Völkner, Philip M. Day, Chance M. Lewis, Chase L. Lewis, Dominik Schneider, Viviana Correa Galvis, Jeffrey A. Cruz, Ute Armbruster, David M. Kramer, Hans‐Henning Kunz

“…Moreover, our study renews calls for caution when setting up reverse genetic studies using T‐DNA lines. Although second‐site insertions, indels, and SNPs have been reported before, large deletion surrounding the insertion site causes yet another problem. …”
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CRISPR/Cas9-Mediated Mutation in <em>XSP10</em> and <em>SlSAMT</em> Genes Impart Genetic Tolerance to Fusarium Wilt Disease of Tomato (<em>Solanum lycopersicum</em> L.) by Johni Debbarma, Banashree Saikia, Dhanawantari L. Singha, Debajit Das, Ajay Kumar Keot, Jitendra Maharana, Natarajan Velmurugan, Kallare P. Arunkumar, Palakolanu Sudhakar Reddy, Channakeshavaiah Chikkaputtaiah

“…Taken together, the reverse genetic studies in transient and stable lines of tomato revealed that, <i>XSP10</i> and <i>SlSAMT</i> function together as negative regulators in conferring genetic tolerance to Fusarium wilt disease.…”
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Role of CIV NS1 Protein in Innate Immunity and Viral Replication by Cheng Fu, Wenhui Zhu, Nan Cao, Wenjun Liu, Zhier Lu, Ziyuan Wong, Kaiting Guan, Chunyan Hu, Baoting Han, Sen Zeng, Shuangqi Fan

“…To further investigate the role of NS1, a recombinant H3N2 virus strain (rH3N2) and an NS1-null virus (rH3N2ΔNS1) were rescued using reverse-genetic technology. The rH3N2ΔNS1 virus exhibited lower viral titers compared to rH3N2, but had a stronger activation effect on the receptors LGP2 and RIG-I. …”
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Attenuated Porcine Reproductive and Respiratory Syndrome Virus Regains Its Fatal Virulence by Serial Passaging in Pigs or Porcine Alveolar Macrophages To Increase Its Adaptation to... by Jingjing Wang, Mengsi Zhang, Xiaochun Cui, Xiang Gao, Weifeng Sun, Xinna Ge, Yongning Zhang, Xin Guo, Jun Han, Lei Zhou, Hanchun Yang

“…Next, the full-genome sequences were analyzed to explore the genetic evolutionary processes during adaptation both in vivo and in vitro. Finally, by a reverse genetic operation, four reverse mutation sites, NSP12-W121R, ORF2b (open reading frame 2b)-H9D, ORF5-H15L, and ORF5-V189L, were finally identified to partially contribute to the ability of the virus to adapt to PAMs, which may be related to virulence reversion during reverse passage. …”
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Comprehensive meta-QTL analysis for dissecting the genetic architecture of stripe rust resistance in bread wheat by Sandeep Kumar, Dinesh Kumar Saini, Farkhandah Jan, Sofora Jan, Mohd Tahir, Ivica Djalovic, Dragana Latkovic, Mohd Anwar Khan, Sundeep Kumar, V. K. Vikas, Upendra Kumar, Sundip Kumar, Narendra Singh Dhaka, Om Parkash Dhankher, Sachin Rustgi, Reyazul Rouf Mir

“…The candidate genes identified can also be utilized for enhancing the wheat resistance against stripe rust after in vivo confirmation/validation using one or more of the following methods: gene cloning, reverse genetic methods, and omics approaches.…”
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A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges by Lei Tan, Guoyuan Wen, Xusheng Qiu, Yanmei Yuan, Chunchun Meng, Yingjie Sun, Ying Liao, Cuiping Song, Weiwei Liu, Yonghong Shi, Huabin Shao, Chan Ding

“…In this study, we successfully generated a recombinant LaSota candidate vaccine strain, rNDV-IBV-T/B, which expresses a short, synthetic, previously identified IBV S1 multi-epitope cassette using the reverse genetic system. The recombinant virus was propagated in nine-day-old embryonated chicken eggs for 20 passages and genetic stability was confirmed by whole genome DNA sequencing. …”
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Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to met... by Sivakumar Ramamoorthy, Michelle Pena, Palash Ghosh, Ying-Yu Liao, Mathews Paret, Jeffrey B. Jones, Neha Potnis

“…IMPORTANCE T6SS has received attention due to its significance in mediating interorganismal competition through contact-dependent release of effector molecules into prokaryotic and eukaryotic cells. Reverse-genetic studies have indicated the role of T6SS in virulence in a variety of plant pathogenic bacteria, including the one studied here, Xanthomonas. …”
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Drosophila TRPA1 controls thermotactic behavior by Rosenzweig, Mark, Ph. D. Massachusetts Institute of Technology

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Uncharacterized and lineage-specific accessory genes within the Proteus mirabilis pan-genome landscape by Robert F. Potter, Kailun Zhang, Ben Reimler, Jamie Marino, Carol E. Muenks, Kelly Alvarado, Meghan A. Wallace, Lars F. Westblade, Erin McElvania, Melanie L. Yarbrough, David A. Hunstad, Gautam Dantas, Carey-Ann D. Burnham

“…The diverse, whole-genome characterized strain bank from this work can be used in conjunction with reverse genetic and infection models to better understand the impact of accessory genome content on bacterial physiology and pathogenesis of infection.…”
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Immunogenicity and Cross-Protective Efficacy Induced by an Inactivated Recombinant Avian Influenza A/H5N1 (Clade 2.3.4.4b) Vaccine against Co-Circulating Influenza A/H5Nx Viruses by Sara H. Mahmoud, Ahmed A. Khalil, Noura M. Abo Shama, Marwa F. El Sayed, Reem A. Soliman, Naglaa M. Hagag, Nahed Yehia, Mahmoud M. Naguib, Abdel-Sattar Arafa, Mohamed A. Ali, Mounir M. El-Safty, Ahmed Mostafa

“…In this study, three H5Nx vaccines were generated by utilizing the reverse genetic system: rgH5N1_2.3.4.4, rgH5N8_2.3.4.4 and rgH5N1_2.2.1.2. …”
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Characterization of Newcastle disease virus (NDV) isolated from NDV vaccinated broiler farms and investigation of vaccine efficacy against challenge with Velogenic genotype VII NDV by Shahrestani, Kiarash Roohani

“…Through the use of reverse genetic technology, genotype–matched NDV vaccine has been used in certain countries. …”
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Poultry viruses: from threat to therapy by Omar, Abdul Rahman

“…In the area of vaccinology, most of the advances in the development of recombinant vaccines against poultry diseases are based on the development of recombinant viral vectored, DNA plasmid and reverse genetic vaccines. However, it is anticipated that more recombinant based vaccines will be used in the field in the near future. …”
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Challenges in controlling viral diseases of poultry by Omar, Abdul Rahman

“…Recently, a few new live recombinant viral vector vaccines based on avian adenovirus and reverse genetic NDV and AIV are making their way in several countries. …”
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