Molecular and Genetics-Based Systems for Tracing the Evolution and Exploring the Mechanisms of Human Norovirus Infections by Sheng-Chieh Lin, Geng-Hao Bai, Pei-Chun Lin, Chung-Yung Chen, Yi-Hsiang Hsu, Yuan-Chang Lee, Shih-Yen Chen

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In vivo characterization of the novel ebolavirus Bombali virus suggests a low pathogenic potential for humans by B. S. Bodmer, A. Breithaupt, M. Heung, J. E. Brunetti, C. Henkel, J. Müller-Guhl, E. Rodríguez, L. Wendt, S. L. Winter, M. Vallbracht, A. Müller, S. Römer, P. Chlanda, C. Muñoz-Fontela, T. Hoenen, B. Escudero-Pérez

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Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus by Anja Dalmann, Kerstin Wernike, Eric J. Snijder, Nadia Oreshkova, Ilona Reimann, Martin Beer

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Investigating the Role of African Horse Sickness Virus VP7 Protein Crystalline Particles on Virus Replication and Release by Shani Bekker, Christiaan A. Potgieter, Vida van Staden, Jacques Theron

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A Reverse-Genetics Mutational Analysis of the Barley HvDWARF Gene Results in Identification of a Series of Alleles and Mutants with Short Stature of Various Degree and Disturbance in BR Biosynthesis Allowing a New Insight into the Process by Damian Gruszka, Malgorzata Gorniak, Ewelina Glodowska, Ewa Wierus, Jana Oklestkova, Anna Janeczko, Miroslaw Maluszynski, Iwona Szarejko

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Mutated Measles Virus Matrix and Fusion Protein Influence Viral Titer In Vitro and Neuro-Invasion in Lewis Rat Brain Slice Cultures by Johannes Busch, Soroth Chey, Michael Sieg, Thomas W. Vahlenkamp, Uwe G. Liebert

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Exploring the Prospects of Engineered Newcastle Disease Virus in Modern Vaccinology by Muhammad Bashir Bello, Khatijah Yusoff, Aini Ideris, Mohd Hair-Bejo, Abdurrahman Hassan Jibril, Ben P. H. Peeters, Abdul Rahman Omar

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Rapid construction of infectious clones for distinct Newcastle disease virus genotypes by Zuhua Yu, Yuhao Zhang, Zedian Li, Qingzhong Yu, Yanyan Jia, Chuan Yu, Jian Chen, Songbiao Chen, Lei He

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In SARS-CoV-2 delta variants, Spike-P681R and D950N promote membrane fusion, Spike-P681R enhances spike cleavage, but neither substitution affects pathogenicity in hamstersResearch... by Yuri Furusawa, Maki Kiso, Shun Iida, Ryuta Uraki, Yuichiro Hirata, Masaki Imai, Tadaki Suzuki, Seiya Yamayoshi, Yoshihiro Kawaoka

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One-Step Assembly of a PRRSV Infectious cDNA Clone and a Convenient CRISPR/Cas9-Based Gene-Editing Technology for Manipulation of PRRSV Genome by Hejin Zhang, Kaiqi Duan, Yingbin Du, Shaobo Xiao, Liurong Fang, Yanrong Zhou

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Advantages, disadvantages and research progress on strategies for screening and identifying functional genes by LIU Xu, CHANG De, WANG Jun-feng

Subjects: “…functional genes|forward genetics|reverse genetics|bioinformatics|model organism…”
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A comprehensive, improved protocol for generating common bean (Phaseolus vulgaris L.) transgenic hairy roots and their use in reverse-genetics studies. by Ronal Pacheco, Georgina Estrada-Navarrete, Jorge Solis-Miranda, Noreide Nava, M A Juárez-Verdayes, Yolanda Ortega-Ortega, Carmen Quinto

“…Importantly, it includes new, critical steps for generating transgenic hairy roots and using them in molecular analyses based on reverse-genetics approaches. Using this protocol, the expression of two different genes, used as an example, was significantly increased or decreased in approximately 30% of the transformed plants. …”
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Application of CRISPR/Cas9-Based Reverse Genetics in <i>Leishmania braziliensis</i>: Conserved Roles for HSP100 and HSP23 by Vanessa Adaui, Constanze Kröber-Boncardo, Christine Brinker, Henner Zirpel, Julie Sellau, Jorge Arévalo, Jean-Claude Dujardin, Joachim Clos

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Tobacco Rattle Virus as a Tool for Rapid Reverse-Genetics Screens and Analysis of Gene Function in <i>Cannabis sativa</i> L. by Hanan Alter, Reut Peer, Aviv Dombrovsky, Moshe Flaishman, Ben Spitzer-Rimon

“…These results indicate that TRV, despite its limited spread, is an attractive vector for rapid reverse-genetics screens and for the analysis of gene function in cannabis.…”
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NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications by Mohan Vijee, Thomas Sherinmol, Hanjabam Mickey, Chauhan Vineeta, Bodanapu Reddaiah, Gupta Soni, Sreelakshmi Yellamaraju, Sharma Sulabha, Srinivasan Rajeswari, Sharma Rameshwar

“…<p>Abstract</p> <p>Background</p> <p>TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetics procedure for identifying point mutations in selected gene(s) amplified from a mutagenized population using high-throughput detection platforms such as slab gel electrophoresis, capillary electrophoresis or dHPLC. …”
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Research Advances on the Interactions between Rabies Virus Structural Proteins and Host Target Cells: Accrued Knowledge from the Application of Reverse Genetics Systems by Juanbin Yin, Xiangwei Wang, Ruoqing Mao, Zhixiong Zhang, Xin Gao, Yingying Luo, Yuefeng Sun, Xiangping Yin

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Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro by Yu Han, Kejia Lu, Riteng Zhang, Xi Wei, Hanwei Guo, Lina Tong, Lina Tong, Xinglong Wang, Sa Xiao, Haijin Liu, Zengqi Yang

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Infectious Salmon Anemia Virus Infectivity Is Determined by Multiple Segments with an Important Contribution from Segment 5 by Matías Cárdenas, Sofía Michelson, Daniel R. Pérez, Margarita Montoya, Jorge Toledo, Yesseny Vásquez-Martínez, Marcelo Cortez-San Martin

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Construction of a Recombinant Porcine Epidemic Diarrhea Virus Encoding Nanoluciferase for High-Throughput Screening of Natural Antiviral Products by Wan Li, Mengjia Zhang, Huijun Zheng, Peng Zhou, Zheng Liu, Anan Jongkaewwattana, Rui Luo, Qigai He

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A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses by Moushimi Amaya, Randy Yin, Lianying Yan, Viktoriya Borisevich, Bishwo N. Adhikari, Andrew Bennett, Francisco Malagon, Regina Z. Cer, Kimberly A. Bishop-Lilly, Antony S. Dimitrov, Robert W. Cross, Thomas W. Geisbert, Christopher C. Broder

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