The structural basis of RNA-catalyzed RNA polymerization by Shechner, David M, Bartel, David, Shechner, David M.

“…Early life presumably required polymerase ribozymes capable of replicating RNA. Known polymerase ribozymes best approximating such replicases use as their catalytic engine an RNA-ligase ribozyme originally selected from random RNA sequences. …”
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A probabilistic model for the evolution of RNA structure

“…The model, its strengths and its weaknesses are discussed with reference to four examples of functional ncRNA sequences: a riboswitch (guanine), a zipcode (nanos), a splicing factor (U4) and a ribozyme (RNase P). As shown by our visualisations of posterior probability matrices, the selected examples illustrate three different signatures of natural selection that are highly characteristic of ncRNA: (i) co-ordinated basepair substitutions, (ii) co-ordinated basepair indels and (iii) whole-stem indels. …”

The cosmological model of eternal inflation and the transition from chance to biological evolution in the history of life by Koonin Eugene V

“…The currently favored (partial) solution is an RNA world without proteins in which replication is catalyzed by ribozymes and which serves as the cradle for the translation system. …”
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The small RNA complement of adult Schistosoma haematobium. by Andreas J Stroehlein, Neil D Young, Pasi K Korhonen, Ross S Hall, Aaron R Jex, Bonnie L Webster, David Rollinson, Paul J Brindley, Robin B Gasser

“…Of the sncRNAs that were not miRNAs, some related to the spliceosome (n = 21), biogenesis of other RNAs (n = 3) or ribozyme functions (n = 16), whereas most others (n = 3798) were novel ('orphans') with unknown functions.…”
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RNA polymerase II-driven CRISPR-Cas9 system for efficient non-growth-biased metabolic engineering of Kluyveromyces marxianus by Danielle Bever, Ian Wheeldon, Nancy Da Silva

“…The use of a strong RNA polymerase II promoter allows efficient expression of gRNAs flanked by the self-cleaving RNA structures, tRNA and HDV ribozyme, from a single plasmid co-expressing a codon optimized Cas9. …”
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The 100th Anniversary of X-Ray Crystallography by Kojić-Prodić, B., Molčanov, K.

“…Historic examples of structure determination starting from rock salt to complex, biologically important (macro)molecules, such as globular proteins haemoglobin and myoglobin, DNA, vitamin B12 and the recent discovery of ribozyme, illustrate the development of X-ray structural analysis. …”
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Laboratory development of an RNA quantitative RT-PCR assay reporting in international units for hepatitis D virus by Carla Osiowy, Carla Osiowy, Jacqueline Day, Emma R. Lee

“…To this end, a quantitative real-time reverse transcription PCR (qRT-PCR) assay utilizing synthetic RNA calibrators and a conversion factor to quantify HDV RNA in WHO international standard units (IU/mL) was developed and validated.MethodsqRT-PCR primers and probes were selected within the ribozyme region. Thermocycling conditions and reactions were optimized. …”
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Differential activities of the two closely related withanolides, Withaferin A and Withanone: bioinformatics and experimental evidences. by Kirti Vaishnavi, Nishant Saxena, Navjot Shah, Rumani Singh, Kavyashree Manjunath, M Uthayakumar, Shankar P Kanaujia, Sunil C Kaul, Kanagaraj Sekar, Renu Wadhwa

“…We had earlier identified several genes involved in cytotoxicity of i-Extract in human cancer cells by loss-of-function assays using either siRNA or randomized ribozyme library. METHODOLOGY/PRINCIPAL FINDINGS:In the present study, we have employed bioinformatics tools on four genes, i.e., mortalin, p53, p21 and Nrf2, identified by loss-of-function screenings. …”
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Click nucleic acid ligation: applications in biology and nanotechnology. by El-Sagheer, A, Brown, T

“…Using click ligation, we have synthesized hairpin ribozymes up to 100 nucleotides in length and a hammerhead ribozyme with the triazole linkage located at the substrate cleavage site. …”

The Ancient History of Peptidyl Transferase Center Formation as Told by Conservation and Information Analyses by Francisco Prosdocimi, Gabriel S. Zamudio, Miryam Palacios-Pérez, Sávio Torres de Farias, Marco V. José

“…The observed sequence variation does not seem to significantly affect the 3D structure of the PTC ribozyme.…”
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Engineering a Circular Riboregulator in Escherichia coli by William Rostain, Shensi Shen, Teresa Cordero, Guillermo Rodrigo, Alfonso Jaramillo

“…Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia coli. …”
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Multidimensional futuristic approaches to address the pandemics beyond COVID-19 by Shifa Bushra Kotwal, Nidhi Orekondey, Gargi Prasad Saradadevi, Neha Priyadarshini, Navinchandra V. Puppala, Mahak Bhushan, Snehasri Motamarry, Rahul Kumar, Gireesha Mohannath, Ruchi Jain Dey

“…We also discuss underexplored and underutilized antiviral strategies, including ribozyme-mediated RNA-cleaving tools for targeting viral RNA, and recent advances in plant-based platforms for rapid, low-cost, and large-scale production and oral delivery of antiviral agents/vaccines. …”
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Serum microRNAs as Diagnostic Markers in Polycystic Ovary Syndrome: A Narrative Review by K Lavanya, N Palaniappan, VM Vinodhini, Santhi Silambanan

“…The profiles of microRNAs differ depending on the tissue from which they originate. Due to ribozyme activity, microRNAs are persistent and resistant to hydrolysis. …”
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Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass by Rahul Kumar, Troy Kamuda, Roshani Budhathoki, Dan Tang, Huseyin Yer, Yunde Zhao, Yi Li

“…We then used the ZmUbi1 promoter for controlling Cas9 and sgRNA expression in perennial ryegrass. A ribozyme cleavage target site between the Cas9 and sgRNA sequences allowed production of functional Cas9 mRNA and sgRNA after transcription. …”
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Primordial soup or vinaigrette: did the RNA world evolve at acidic pH? by Bernhardt Harold S, Tate Warren P

“…Examples of <it>in vitro</it>-selected ribozymes with activities at acid pH have recently been documented. …”
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<i>Staphylococcus aureus</i> RnpA Inhibitors: Computational-Guided Design, Synthesis and Initial Biological Evaluation by Lorenzo Suigo, Michaelle Chojnacki, Carlo Zanotto, Victor Sebastián-Pérez, Carlo De Giuli Morghen, Andrea Casiraghi, Paul M. Dunman, Ermanno Valoti, Valentina Straniero

“…<i>S. aureus</i> RnpA is able to modulate mRNA degradation and complex with a ribozyme (<i>rnpB</i>), facilitating ptRNA maturation. …”
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Claudin-5 is involved in breast cancer cell motility through the N-WASP and ROCK signalling pathways by Escudero-Esparza Astrid, Jiang Wen G, Martin Tracey A

“…The Claudin-5 gene was cloned and overexpressed or knocked down using ribozyme technology in human breast cancer cells. Changes in function were assessed using <it>in vitro</it> assays for invasion, growth, adhesion, wounding, motility, transepithelial resistance and electric cell-substrate impedance sensing. …”
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Deconvolution of multichannel LC-MS/MS chromatograms of glucosamine-phosphates: Evidence of a GlmS regulatory difference between Staphylococcus aureus and Enterococcus faecium by Nitish R. Mishra, Amar Deep Sharma, Shivani Gargvanshi, William G. Gutheil

“…These observations appear to localize previously observed differences between MRSA and VRE/VSE peptidoglycan biosynthesis regulation to GlmS, which synthesizes GlcN-6P and is the product of a regulatory ribozyme sensitive to the levels of GlcN-6P…”
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Synthetic Tet-inducible artificial microRNAs targeting β-catenin or HIF-1α inhibit malignant phenotypes of bladder cancer cells T24 and 5637 by Yonghao Zhan, Yuchen Liu, Junhao Lin, Xing Fu, Chengle Zhuang, Li Liu, Wen Xu, Jianfa Li, Mingwei Chen, Guoping Zhao, Weiren Huang, Zhiming Cai

“…The vector generates a Pol II promoter-mediated artificial microRNA which was flanked by ribozyme sequences. In order to prove the utility of this platform, we chose β-catenin and HIF-1α as the functional targets and used the bladder cancer cell lines 5637 and T24 as the test models. …”
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RNA recombination in Hepatitis delta virus: Identification of a novel naturally occurring recombinant by Chia-Chi Lin, Chi-Ching Lee, Siao-Han Lin, Po-Jung Huang, Hsin-Pai Li, Yu-Sun Chang, Petrus Tang, Mei Chao

“…Background/Purpose: Hepatitis delta virus (HDV) is the only animal RNA virus that has an unbranched rod-like genome with ribozyme activity. It replicates in the nucleus by host RNA polymerase via a rolling circle mechanism. …”
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