Ribozyme-mediated reduction of wild-type and mutant cartilage oligomeric matrix protein (COMP) mRNA and protein. by Alcorn, J, Merritt, T, Farach-Carson, M, Wang, H, Hecht, J

“…The goal of this study was to assess the ability of a hammerhead ribozyme (Ribo56, designed against the D469del mutation) to reduce COMP mRNA expression. …”

Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication by Yüksel YILDIZ, John J. ROSSI

Subjects: Get full text

Targeted suicide gene therapy for liver cancer based on ribozyme-mediated RNA replacement through post-transcriptional regulation by Seung Ryul Han, Chang Ho Lee, Ji Young Im, Ju Hyun Kim, Ji Hyun Kim, Sung Jin Kim, Young Woo Cho, Eunkyung Kim, Youngah Kim, Ji-Ho Ryu, Mi Ha Ju, Jin Sook Jeong, Seong-Wook Lee

Subjects: Get full text

Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme by Yonghao Zhan, Congcong Cao, Aolin Li, Hongbing Mei, Yuchen Liu

“…We show that the CasRx-crRNA-ribozyme system (CCRS) is more efficient for RNA knockdown of mRNAs and non-coding RNAs than conventional methods, including CasRx, shRNA, and ribozyme. …”
Get full text

RNase footprinting demonstrates antigenomic hepatitis delta virus ribozyme structural rearrangement as a result of self-cleavage reaction by Dobrynina Nadezhda, Belyanko Tatyana, Alekseenkova Victoria, Savochkina Larissa, Beabealashvilli Robert

“…We have used ribonuclease probing to footprint the structures of uncleaved and post-cleaved forms of the antigenomic HDV ribozymes in solution. Uncleaved ribozymes, associated and individual products of the self-cleavage reaction were analyzed using ribonuclease and Fe(II)-EDTA protection assays to reveal the differences in the structure of pre- and post-cleaved antigenomic HDV ribozyme in solution.…”
Get full text

Selective cleavage of AChR cRNAs harbouring mutations underlying the slow channel myasthenic syndrome by hammerhead ribozymes. by Abdelgany, A, Ealing, J, Wood, M, Beeson, D

“…Ribozymes were incubated with cRNA transcripts encoding wild type and mutant AChR subunits. …”

Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs by Airi Furukawa, Takahiro Tanaka, Hiroyuki Furuta, Shigeyoshi Matsumura, Yoshiya Ikawa

Subjects: Get full text

Oligonucleotide-Based Approaches to Inhibit Dengue Virus Replication by Kingshuk Panda, Kalichamy Alagarasu, Deepti Parashar

Subjects: Get full text

Antisense technology as a potential strategy for the treatment of coronaviruses infection: With focus on COVID‐19 by Seyedeh Hoda Alavizadeh, Maham Doagooyan, Fatemeh Zahedipour, Shima Yahoo Torghabe, Bahare Baharieh, Firooze Soleymani, Fatemeh Gheybi

Subjects: Get full text

Rolling circle RNA synthesis catalyzed by RNA by Emil Laust Kristoffersen, Matthew Burman, Agnes Noy, Philipp Holliger

Subjects: Get full text

Ribozyme-Mediated Inhibition of 801-bp Deletion-Mutant Epidermal Growth Factor Receptor mRNA Expression in Glioblastoma Multiforme by Georg Karpel-Massler, Christian Rainer Wirtz, Marc-Eric Halatsch

Subjects: Get full text

Structural Insights Into Tautomeric Dynamics in Nucleic Acids and in Antiviral Nucleoside Analogs by Bogdan I. Fedeles, Deyu Li, Vipender Singh

Subjects: Get full text

Viroids and Viroid-like Circular RNAs: Do They Descend from Primordial Replicators? by Benjamin D. Lee, Eugene V. Koonin

Subjects: Get full text

Novel Fig-Associated Viroid-Like RNAs Containing Hammerhead Ribozymes in Both Polarity Strands Identified by High-Throughput Sequencing by Alejandro Olmedo-Velarde, Beatriz Navarro, John S. Hu, Michael J. Melzer, Francesco Di Serio

Subjects: Get full text

Development of a Functionally Minimized Mutant of the R3C Ligase Ribozyme Offers Insight into the Plausibility of the RNA World Hypothesis by Eri Kurihara, Sayuri Uchida, Takuya Umehara, Koji Tamura

Subjects: “…R3C ligase ribozyme…”
Get full text

Efficient circular RNA engineering by end-to-end self-targeting and splicing reaction using Tetrahymena group I intron ribozyme by Kyung Hyun Lee, Seongcheol Kim, Jaehwi Song, Seung Ryul Han, Ji Hyun Kim, Seong-Wook Lee

“…In this study, we developed a new in vitro circRNA engineering method by end-to-end self-targeting and splicing (STS) reaction using Tetrahymena group I intron ribozyme. We found that only the P1 helix structure of the group I intron was enough to generate circRNA by STS reaction. …”
Get full text

RNase P Ribozyme Effectively Inhibits Human CC-Chemokine Receptor 5 Expression and Human Immunodeficiency Virus 1 Infection by Bin Yan, Yujun Liu, Yuan-Chuan Chen, Isadora Zhang, Fenyong Liu

“…Here, engineered ribozymes were constructed from an RNase P ribozyme variant to target the mRNA encoding human CC-chemokine receptor 5 (CCR5), an HIV co-receptor. …”
Get full text

Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome by Mohammed Ahmed, Higgs Stephen, Davey Robert A, Kolokoltsov Andrey, Balaraman Velmurugan, Fraser Tresa, Keith James, Nawtaisong Pruksa, Rongsriyam Yupha, Komalamisra Narumon, Fraser Malcolm J

“…A lentivirus vector that expresses these ribozymes as a fusion RNA molecule using an <it>Ae. aegypti </it>tRNA^valpromoter and terminating with a 60A tail insures optimal expression, localization, and activity of the hammerhead ribozyme against the DENV genome. …”
Get full text

Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans by Jie Fang, Jie Wang, Yuzhi Wang, Xiaofan Liu, Baohui Chen, Wei Zou

“…Conditional endogenous gene turn-on can be achieved by either injecting morpholino, which blocks the ribozyme self-cleavage activity or using the Cre recombinase to remove the loxP-flanked ribozyme. …”
Get full text

Hammerhead ribozyme specifically inhibits vascular endothelial growth factor gene expression in a human hepatocellular carcinoma cell line. by Morino, F, Tokunaga, T, Tsuchida, T, Handa, A, Nagata, J, Tomii, Y, Kijima, H, Yamazaki, H, Watanabe, N, Matsuzaki, S, Ueyama, Y, Nakamura, M

“…Hammerhead-type ribozymes are often utilized to suppress the expression of target genes. …”