Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains by Galina V. Beznoussenko, Hee-Seok Kweon, Irina S. Sesorova, Alexander A. Mironov

“…The Golgi complex is the central station of the secretory pathway. Knowledge about the mechanisms of intra-Golgi transport is inconsistent. …”
Get full text

Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization by Danique M. van Bommel, Ruud F. Toonen, Matthijs Verhage

“…Our data underline the importance of retrograde trafficking for Golgi organization, DCV cargo sorting and the distribution of proteins of the regulated secretory pathway.…”
Get full text

Life and Death of Fungal Transporters under the Challenge of Polarity by Sofia Dimou, George Diallinas

“…Sorting of transporters from their site of synthesis, the endoplasmic reticulum (ER), to the PM has been long thought, but not formally shown, to occur via the conventional Golgi-dependent vesicular secretory pathway. Endocytosis of specific eukaryotic transporters has been studied more systematically and shown to involve ubiquitination, internalization, and sorting to early endosomes, followed by turnover in the multivesicular bodies (MVB)/lysosomes/vacuole system. …”
Get full text

The microneme proteins EtMIC4 and EtMIC5 of Eimeria tenella form a novel, ultra-high molecular mass protein complex that binds target host cells. by Periz, J, Gill, A, Hunt, L, Brown, P, Tomley, F

“…The complex is formed within the parasite secretory pathway and is maintained after secretion onto the surface of the parasite. …”

The Menkes disease ATPase (ATP7A) is internalized via a Rac1-regulated, clathrin- and caveolae-independent pathway. by Cobbold, C, Coventry, J, Ponnambalam, S, Monaco, A

“…The Menkes disease gene encodes a P-type transmembrane ATPase (ATP7A) that translocates cytosolic copper ions across intracellular membranes of compartments along the secretory pathway. ATP7A moves from the trans-Golgi network (TGN) to the cell surface in response to exogenously added copper ions and recycles back to the TGN upon copper removal. …”

Mechanisms of substrate processing during ER-associated protein degradation by Christianson, JC, Jarosch, E, Sommer, TJ

“…Maintaining proteome integrity is essential for long-term viability of all organisms and is overseen by intrinsic quality control mechanisms. The secretory pathway of eukaryotes poses a challenge for such quality assurance as proteins destined for secretion enter the endoplasmic reticulum (ER) and become spatially segregated from the cytosolic machinery responsible for disposal of aberrant (misfolded or otherwise damaged) or superfluous polypeptides. …”

An overexpression screen of Toxoplasma gondii Rab-GTPases reveals distinct transport routes to the micronemes by Kremer, K, Kamin, D, Rittweger, E, Wilkes, J, Flammer, H, Mahler, S, Heng, J, Tonkin, C, Langsley, G, Hell, S, Carruthers, V, Ferguson, D, Meissner, M

“…In order to define the secretory pathway of apicomplexans, we performed an overexpression screen of Rabs in Toxoplasma gondii and identified Rab5A and Rab5C as important regulators of traffic to micronemes and rhoptries. …”

Functional organisation of the Hrd1 ubiquitin ligase complex and its role in ERAD by Schulz, J

“…<p>Endoplasmic reticulum (ER)-associated degradation (ERAD) of misfolded proteins of the secretory pathway is crucial for ER homeostasis and the physiological importance of this mechanism is reflected by more than 60 diseases that have been linked to ERAD to date. …”

Extracellular Glycolytic Activities in Root Endophytic <i>Serendipitaceae</i> and Their Regulation by Plant Sugars by Vincenzo De Rocchis, Thomas Roitsch, Philipp Franken

“…Since no conventional signal peptide could be detected in most of the genome sequences encoding the glycolytic enzymes, a non-conventional protein secretory pathway is assumed. The results of the study suggest that root endophytic fungi translocate glycolytic activities into the root, and this process is regulated by the availability of particular plant sugars.…”
Get full text

Codon Bias Can Determine Sorting of a Potassium Channel Protein by Anja J. Engel, Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L. Van Etten, Anna Moroni, Gerhard Thiel

“…By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.…”
Get full text

Engineering Approaches in Plant Molecular Farming for Global Health by Advaita Acarya Singh, Priyen Pillay, Tsepo Lebiletsa Tsekoa

“…The plant system offers a faster alternative, which is a potentially more cost-effective and scalable platform for the mass production of highly complex protein vaccines, owing to the high degree of similarity between the plant and mammalian secretory pathway. Here, we identify and address bottlenecks in the use of plants for vaccine manufacturing and discuss engineering approaches that demonstrate both the utility and versatility of the plant production system as a viable biomanufacturing platform for global health. …”
Get full text

Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation by Zinia D’Souza, Farhana Taher Sumya, Amrita Khakurel, Vladimir Lupashin

“…The Golgi is the central organelle of the secretory pathway and it houses the majority of the glycosylation machinery, which includes glycosylation enzymes and sugar transporters. …”
Get full text

Pancreatic beta cell ER export in health and diabetes by Cesar Barrabi, Kezhong Zhang, Ming Liu, Xuequn Chen

“…In the secretory pathway of the pancreatic beta cell, proinsulin and other secretory granule proteins are first produced in the endoplasmic reticulum (ER). …”
Get full text

A survey of putative secreted and transmembrane proteins encoded in the <it>C. elegans</it> genome by Suh Jinkyo, Hutter Harald

“…Therefore a substantial fraction of the proteins are localized to membranes, reside in the secretory pathway or are secreted. While these proteins are of interest to a variety of different researchers ranging from developmental biologists to immunologists, most of secreted proteins have not been functionally characterized so far.…”
Get full text

An outlook to sophisticated technologies and novel developments for metabolic regulation in the Saccharomyces cerevisiae expression system by Yijian Wu, Sai Feng, Zeao Sun, Yan Hu, Xiao Jia, Bin Zeng

“…In addition to the widely applied promoter engineering technology and the clear mechanism of the endoplasmic reticulum secretory pathway, the innovative genome editing technique CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated system) and its derivative tools allow for more precise and efficient gene disruption, site-directed mutation, and foreign gene insertion. …”
Get full text

An ambiguous N-terminus drives the dual targeting of an antioxidant protein Thioredoxin peroxidase (TgTPx1/2) to endosymbiotic organelles in Toxoplasma gondii by Pragati Mastud, Swati Patankar

“…Nuclear-encoded proteins such as TgTPx1/2 are trafficked to the apicoplast via a secretory route through the endoplasmic reticulum (ER) and to the mitochondrion via a non-secretory pathway comprising of translocon uptake. Given the two distinct trafficking pathways for localization to the two organelles, the signals in TgTPx1/2 for this dual targeting are open areas of investigation. …”
Get full text

New putative chloroplast vesicle transport components and cargo proteins revealed using a bioinformatics approach: an Arabidopsis model. by Nadir Zaman Khan, Emelie Lindquist, Henrik Aronsson

“…This protein is localized in the donor membrane (envelope) and vesicles, but not in the target membrane (thylakoids) suggesting it plays a similar role to a cytosolic homologue, Sar1, in the secretory pathway. Thus, we hypothesized that there may be more similarities, in addition to lipid transport, between the vesicle transport systems in the cytosol and chloroplast, i.e. similar vesicle transport components, possible cargo proteins and receptors. …”
Get full text

Inhibitors of the Sec61 Complex and Novel High Throughput Screening Strategies to Target the Protein Translocation Pathway by Eva Pauwels, Ralf Schülein, Kurt Vermeire

“…Proteins targeted to the secretory pathway start their intracellular journey by being transported across biological membranes such as the endoplasmic reticulum (ER). …”
Get full text

Copper-Zinc Superoxide Dismutase (SOD1) Is Released by Microglial Cells and Confers Neuroprotection against 6-OHDA Neurotoxicity by Elisabetta Polazzi, Ilaria Mengoni, Marco Caprini, Emiliano Peña-Altamira, Ewelina Kurtys, Barbara Monti

“…SOD1, constitutively produced and released by microglia through a lysosomal secretory pathway, is identified here for the first time as an essential component of neuroprotection mediated by microglia. …”
Get full text

LncRNAs and CircRNAs in Endoplasmic Reticulum Stress: A Promising Target for Cardiovascular Disease? by Francisco José Martinez-Amaro, Carlos Garcia-Padilla, Diego Franco, Houria Daimi

“…The endoplasmic reticulum (ER) is a principal subcellular organelle responsible for protein quality control in the secretory pathway, preventing protein misfolding and aggregation. …”
Get full text