Tumour Progression Stage-Dependent Secretion of YB-1 Stimulates Melanoma Cell Migration and Invasion by Corinna Kosnopfel, Tobias Sinnberg, Birgit Sauer, Heike Niessner, Alina Muenchow, Birgit Fehrenbacher, Martin Schaller, Peter R. Mertens, Claus Garbe, Basant Kumar Thakur, Birgit Schittek

“…Intriguingly, YB-1 secretion correlates with the stage of melanoma progression and depends on a calcium- and ATP-dependent non-classical secretory pathway leading to the occurrence of YB-1 in the extracellular space as a free protein. …”
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A plant protein signal sequence improved humoral immune response to HPV prophylactic and therapeutic DNA vaccines by Silvia Massa, Francesca Paolini, Gianfranca Curzio, Marcelo Nazario Cordeiro, Elena Illiano, Olivia Costantina Demurtas, Rosella Franconi, Aldo Venuti

“…Thus, a plant ss was used to drive Human Papillomavirus (HPV) model antigens into the human secretory pathway: the HPV16 E7 oncoprotein, its chimera with the coat protein (CP) of the Potato Virus X (PVX), the first 200 amino acids of the HPV16 minor capsid protein L2 (known to harbour cross-reacting epitopes) and its chimera with E7 gene. …”
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Redox Homeostasis in Pancreatic β-Cells: From Development to Failure by Štěpánka Benáková, Blanka Holendová, Lydie Plecitá-Hlavatá

“…We briefly highlight the targets of redox signaling in the insulin secretory pathway and focus more on possible targets of extracellular redox signaling through secreted thioredoxin1 and thioredoxin reductase1. …”
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Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into <it>Pichia pastoris </it>producing high levels of the Hepatitis B surface antigen by Adnan Ahmad, Gurramkonda Chandrasekhar, Lünsdorf Heinrich, Khanna Navin, Rinas Ursula

“…Further transport of HBsAg to the Golgi apparatus is impaired thus leading to secretory pathway disfunction and the formation of an extended endoplasmic reticulum which bulges into irregular cloud-shaped formations. …”
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To be or not to be a fat burner, that is the question for cpt1c in cancer cells by Rut Fadó, Sebastian Zagmutt, Laura Herrero, Helena Muley, Rosalía Rodríguez-Rodríguez, Huichang Bi, Dolors Serra, Núria Casals

“…In this review, we collate existing evidence on CPT1C in neurons, showing that CPT1C is a sensor of nutrients that interacts with and regulates other proteins involved in lipid metabolism and transport, lysosome motility, and the secretory pathway. We argue, therefore, that CPT1C expression in cancer cells is not a direct regulator of fat burn, but rather is a regulator of lipid metabolic reprograming and cell adaptation to environmental stressors. …”
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Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris by Olufemi Emmanuel Bankefa, Meiyu Wang, Taicheng Zhu, Yin Li

“…Moreover, separate applications of the mixed feeding strategy and secretory pathway engineering only achieved limited success in enhancing Gox levels, while the combined use of the two strategies acted synergistically, leading to 297% increase of Gox production and the final enzyme titer reached 787.4 U/mL in GSgox-Pp on 1-L fermenter. …”
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Genetically Encoded Fluorescent Probes for Imaging of Intracellular Localization and Activity of SARS-CoV-2 Proteins by Elena Sokolinskaya, Lidia Putlyaeva, Konstantin Lukyanov

“…We found that the M protein localizes in cell lysosomes, which supports the recent hypothesis that β-coronaviruses use lysosomal organelles for egress instead of the traditional Golgi-mediated secretory pathway. Further, we plan to study the interactions between M, E, and S using the FRET method. …”
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SLC26A9 in airways and intestine: secretion or absorption? by Karl Kunzelmann, Raquel Centeio, Jiraporn Ousingsawat, Khaoula Talbi, Ursula Seidler, Rainer Schreiber

“…SLC26A9 supports duodenal bicarbonate secretion, but was assumed to provide a basal Cl- secretory pathway in airways. However, recent results show that basal airway Cl- secretion is due to cystic fibrosis conductance regulator (CFTR), while SLC26A9 may rather secrete HCO3-, thereby maintaining proper airway surface liquid (ASL) pH. …”
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Insulin secretion deficits in a Prader-Willi syndrome β-cell model are associated with a concerted downregulation of multiple endoplasmic reticulum chaperones. by Erik A Koppes, Marie A Johnson, James J Moresco, Patrizia Luppi, Dale W Lewis, Donna B Stolz, Jolene K Diedrich, John R Yates, Ronald C Wek, Simon C Watkins, Susanne M Gollin, Hyun Jung Park, Peter Drain, Robert D Nicholls

“…In summary, our results illuminate the pathophysiological basis of pancreatic β-cell hormone deficits in PWS, with evolutionary implications for the multigenic PWS-domain, and indicate that PWS-imprinted genes coordinate concerted regulation of ER chaperone biosynthesis and β-cell secretory pathway function.…”
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Synthetic pro‐peptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae by Ji Sung Cho, Hye Ji Oh, Young Eun Jang, Hyun Jin Kim, Areum Kim, Jong‐Am Song, Eun Jung Lee, Jeewon Lee

“…To improve the production of the heterologous protein, human granulocyte colony‐stimulating factor (hG‐CSF) in S. cerevisiae, a secretion‐enhancing peptide cassette including an hIL‐1β‐derived pro‐peptide, was added and used as a secretion enhancer to alleviate specific bottlenecks in the yeast secretory pathway. The effects of three key parameters—N‐glycosylation, net negative charge balance, and glycine‐rich flexible linker—were investigated in batch cultures of S. cerevisiae. …”
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Reticulocalbin 3 Is a Novel Mediator of Glioblastoma Progression by Yi He, Salvador Alejo, Jessica D. Johnson, Sridharan Jayamohan, Gangadhara R. Sareddy

“…Reticulocalbin 3 (RCN3), an ER lumen-residing Ca²⁺ binding protein, plays an essential role in protein biosynthesis processes via the secretory pathway. Emerging studies demonstrated that RCN3 is a target for therapeutic intervention in various diseases. …”
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Epigenetic Modulation of SPCA2 Reverses Epithelial to Mesenchymal Transition in Breast Cancer Cells by Monish Ram Makena, Myungjun Ko, Donna Kimberly Dang, Rajini Rao

“…The secretory pathway Ca²⁺-ATPase SPCA2 is a tumor suppressor in triple receptor negative breast cancer (TNBC), a highly aggressive molecular subtype that lacks tailored treatment options. …”
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ER stress as a trigger of UPR and ER-phagy in cancer growth and spread by Alessandro Cherubini, Alessandro Cherubini, Ester Zito, Ester Zito

“…The endoplasmic reticulum (ER) is the organelle where proteins undergo post-translational modifications and are folded and exported to the secretory pathway. Its environment and activity are therefore fundamental for proteostasis, i.e., the plethora of mechanisms controlling protein formation, folding, degradation, and secretion, needed to assure protein balance and cellular health. …”
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Receptor and secreted targets of Wnt-1/β-catenin signalling in mouse mammary epithelial cells by Enver Tariq, Kenny Paraic A, Ashworth Alan

“…Consistent with this, a Hig2-EGFP fusion protein was able to enter the secretory pathway and was detected in conditioned medium. …”
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CircRNA Expression Profiles in Canine Mammary Tumours by Yufan Zhu, Baochun Lu, Juye Wu, Shoujun Li, Kun Jia

“…The GO analysis revealed that the biological processes involved were mainly in the regulation of the secretory pathway, the regulation of neurotransmitter secretion and the positive regulation of phagocytosis. …”
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ESCRT-III drives the final stages of CUPS maturation for unconventional protein secretion by Amy J Curwin, Nathalie Brouwers, Manuel Alonso Y Adell, David Teis, Gabriele Turacchio, Seetharaman Parashuraman, Paolo Ronchi, Vivek Malhotra

“…The unconventional secretory pathway exports proteins that bypass the endoplasmic reticulum. …”
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The Possible Role of the Type I Chaperonins in Human Insulin Self-Association by Federica Pizzo, Maria Rosalia Mangione, Fabio Librizzi, Mauro Manno, Vincenzo Martorana, Rosina Noto, Silvia Vilasi

“…The group I chaperonin cpn60, known for its assembly-assisting function, is present, together with its cochaperonin cpn10, at each step of the insulin secretory pathway. However, the exact function of the heat shock protein in insulin biosynthesis and processing is still far from being understood. …”
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Pyrosequencing-based comparative genome analysis of Vibrio vulnificus environmental isolates. by Shatavia S Morrison, Tiffany Williams, Aurora Cain, Brett Froelich, Casey Taylor, Craig Baker-Austin, David Verner-Jeffreys, Rachel Hartnell, James D Oliver, Cynthia J Gibas

“…Genes specific to the clinical strains include components of sialic acid catabolism, mannitol fermentation, and a component of a Type IV secretory pathway VirB4, as well as several other genes with potential significance for human virulence. …”
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The journey of cardosin A in young Arabidopsis seedlings leads to evidence of a Golgi-independent pathway to the protein storage vacuole by Cláudia Pereira, Vanessa Vieira, José Pissarra, Susana Pereira

“…Using antibodies against different regions of the protein and combining them with immunofluorescence and immunocytochemistry assays in different young seedling tissues, cardosin A was detected along the secretory pathway to the protein storage vacuole, often associated with the endoplasmic reticulum. …”
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Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures by Mamosa G. Ngcala, Tatenda Goche, Adrian P. Brown, Stephen Chivasa, Rudo Ngara

“…Of the 265 secreted proteins identified, 31 responded to heat (≥2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. …”
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