In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in <i>Escherichia coli</i> by Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner, Winfried Römer, W. Bruce Turnbull, Juergen Mairhofer

“…By an in-depth characterization of the production process, we revealed that secretion of rCTC across the <i>E. coli</i> Outer Membrane (OM) is processed by the Type II secretion-system general secretory pathway (gsp-operon) and that cholera toxin B-pentamerization is, likely, the rate-limiting step in complex formation. …”
Get full text

The degradation of membrane proteins from the mammalian endoplasmic reticulum by Stern, Patrick J. (Patrick Joseph), 1972-

Get full text

The antigen processing-associated transporter gene polymorphism: Role on gene and protein expression in HPV-infected pre-cancerous cervical lesion by Fernanda Silva Medeiros, Mauro César da Silva, Neila Caroline Henrique da Silva, Thailany Thays Gomes, Renan Garcia Gomes, Larissa Albuquerque Paiva, Fabiana Oliveira dos Santos Gomes, Christina Alves Peixoto, Maria Carolina Valença Rygaard, Stefan Welkovic, Maria Luiza Bezerra Menezes, Eduardo Antônio Donadi, Norma Lucena-Silva, Norma Lucena-Silva

“…The evasion mechanism of the immune response and persistence of HPV infection can be influenced by polymorphisms (SNPs) in genes associated with transporter associated with antigen processing (TAP), which may change the peptide binding affinity or the TAP expression impacting the efficiency of peptide transport in the secretory pathway, and the presentation of peptides to cytotoxic T lymphocytes. …”
Get full text

PERK-Mediated Unfolded Protein Response Activation and Oxidative Stress in PARK20 Fibroblasts by Giuseppina Amodio, Ornella Moltedo, Dominga Fasano, Lucrezia Zerillo, Marco Oliveti, Paola Di Pietro, Raffaella Faraonio, Paolo Barone, Maria Teresa Pellecchia, Anna De Rosa, Giuseppe De Michele, Elena Polishchuk, Roman Polishchuk, Vincenzo Bonifati, Lucio Nitsch, Giovanna Maria Pierantoni, Maurizio Renna, Chiara Criscuolo, Simona Paladino, Paolo Remondelli

“…All together, these findings suggest that dysfunction of early secretory pathway might contribute to the pathogenesis of the disease.…”
Get full text

BIG3 and BIG5 Redundantly Mediate Vesicle Trafficking in <i>Arabidopsis</i> by Yiping Suo, Fenhong Hu, Haojie Zhu, Di Li, Rui Qi, Jirong Huang, Wenjuan Wu

“…Among the five BIGs in <i>Arabidopsis</i>, BIG5 is characterized to mediate ARF-dependent trafficking at the plasma membrane or endosomes while the members from BIG1 to BIG4 (BIG1-BIG4) at the TGN in the secretory pathway. However, evidence is increasing to suggest that BIG5 can function redundantly with BIG1-BIG4 to regulate vesicular trafficking in response to various intra- and extra-cellular stimuli. …”
Get full text

Human Respiratory Syncytial Virus Infection in a Human T Cell Line Is Hampered at Multiple Steps by Ricardo de Souza Cardoso, Rosa Maria Mendes Viana, Brenda Cristina Vitti, Ana Carolina Lunardello Coelho, Bruna Laís Santos de Jesus, Juliano de Paula Souza, Marjorie Cornejo Pontelli, Tomoyuki Murakami, Armando Morais Ventura, Akira Ono, Eurico Arruda

“…We also assessed the intracellular trafficking of HRSV proteins and found that HRSV proteins colocalized partially with the secretory pathway in A3.01 cells, but these HRSV proteins and viral filaments were present only scarcely at the plasma membrane. …”
Get full text

Defining the ligand-dependent proximatome of the sigma 1 receptor by Jing Zhao, Jing Zhao, Rajalakshmi Veeranan-Karmegam, Frederick C. Baker, Barbara A. Mysona, Barbara A. Mysona, Barbara A. Mysona, Pritha Bagchi, Yutao Liu, Yutao Liu, Sylvia B. Smith, Sylvia B. Smith, Sylvia B. Smith, Graydon B. Gonsalvez, Kathryn E. Bollinger, Kathryn E. Bollinger, Kathryn E. Bollinger

“…Under native conditions, S1R biotinylates over 200 novel proteins, many of which localize within the endomembrane system (endoplasmic reticulum, Golgi, secretory vesicles) and function within the secretory pathway. Under conditions of cellular exposure to either S1R agonist or antagonist, results show enrichment of proteins integral to secretion, extracellular matrix formation, and cholesterol biosynthesis. …”
Get full text

PIMT Controls Insulin Synthesis and Secretion through PDX1 by Rahul Sharma, Sujay K. Maity, Partha Chakrabarti, Madhumohan R. Katika, Satyamoorthy Kapettu, Kishore V. L. Parsa, Parimal Misra

“…The RNA sequencing analysis of PIMT knockdown beta cells identified that the expression of key genes involved in insulin secretory pathway, <i>Ins1</i> (insulin 1), <i>Ins2</i> (insulin 2), <i>Kcnj11</i> (potassium inwardly-rectifying channel, subfamily J, member 11), <i>Kcnn1</i> (potassium calcium-activated channel subfamily N member 1), <i>Rab3a</i> (member RAS oncogene family), <i>Gnas</i> (GNAS complex locus), <i>Syt13</i> (synaptotagmin 13), <i>Pax6</i> (paired box 6), <i>Klf11</i> (Kruppel-Like Factor 11), and <i>Nr4a1</i> (nuclear receptor subfamily 4, group A, member 1) was attenuated due to PIMT depletion. …”
Get full text

The <i>Sporisorium reilianum</i> Effector Vag2 Promotes Head Smut Disease via Suppression of Plant Defense Responses by Yulei Zhao, Nisha Agrawal, Hassan Ghareeb, Mohammad Tanbir Habib, Sascha Dickmeis, Jens Schwachtje, Tim E. Iven, Joachim Kopka, Ivo Feussner, Jan Schirawski

“…Using the yeast secretion trap assay, we confirmed the existence of a functional signal peptide allowing protein secretion via the conventional secretory pathway. We identified the cytoplasmic maize chorismate mutase ZmCM2 by yeast two-hybrid screening as a possible interaction partner of Vag2. …”
Get full text

The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus by Hui Qian, Lixiao Sun, Minghua Wu, Wenhui Zhao, Mengyu Liu, Shuang Liang, Xueming Zhu, Lin Li, Zhenzhu Su, Jianping Lu, Fucheng Lin, Fucheng Lin, Xiaohong Liu

“…The endoplasmic reticulum (ER) acts as the starting point of the secretory pathway, where approximately one-third of the proteins are correctly folded and modified, loaded into vesicles, and transported to the Golgi for further processing and modification. …”
Get full text

Dual role of neddylation in transcription of hepatitis B virus RNAs from cccDNA and production of viral surface antigen by Bingqian Qu, Firat Nebioglu, Mila M. Leuthold, Yi Ni, Pascal Mutz, Jürgen Beneke, Holger Erfle, Florian W.R. Vondran, Ralf Bartenschlager, Stephan Urban

“…Methods: An siRNA screen targeting secretory pathway regulators and neddylation genes was performed. …”
Get full text

African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway by Han Zhong, Shuai Fan, Yongkun Du, Yuhang Zhang, Angke Zhang, Dawei Jiang, Shichong Han, Bo Wan, Gaiping Zhang

“…Notably, our subsequent analyses confirmed that MGF110-7L was overwhelmingly retained in the ER and caused a specific reorganization of the secretory pathway. Further proteomic analyses and biochemical experiments revealed that MGF110-7L could trigger ER stress and activate the unfolded protein response, thus contributing to eIF2α phosphorylation and translation reprogramming. …”
Get full text

Requirement of microtubules for secretion of a micronemal protein CpTSP4 in the invasive stage of the apicomplexan Cryptosporidium parvum by Dongqiang Wang, Peng Jiang, Xiaodong Wu, Ying Zhang, Chenchen Wang, Meng Li, Mingxiao Liu, Jigang Yin, Guan Zhu

“…However, the understanding of cryptosporidial micronemal composition and secretory pathway remains limited. Here, we report a new micronemal protein in C. parvum, namely, thrombospondin (TSP)-repeat domain-containing protein-4 (CpTSP4), providing insights into these ambiguities. …”
Get full text

Genetic regulation of secretory granule and exosome biogenesis within Drosophila secondary cells by Wells, A

“…<p>Secretory pathways are an essential feature of cell-cell signalling processes, yet many fundamental questions remain unanswered concerning the regulatory mechanisms controlling the biogenesis and maturation of the small secretory compartments generated in most cells. …”

A plant-produced SARS-CoV-2 spike protein elicits heterologous immunity in hamsters by Emmanuel Margolin, Emmanuel Margolin, Emmanuel Margolin, Emmanuel Margolin, Georgia Schäfer, Georgia Schäfer, Georgia Schäfer, Joel D. Allen, Sophette Gers, Jeremy Woodward, Andrew D. Sutherland, Melissa Blumenthal, Melissa Blumenthal, Ann Meyers, Megan L. Shaw, Wolfgang Preiser, Richard Strasser, Max Crispin, Anna-Lise Williamson, Anna-Lise Williamson, Edward P. Rybicki, Edward P. Rybicki, Ros Chapman, Ros Chapman

“…Remodelling the secretory pathway presents an opportunity to support key post-translational modifications, and to tailor aspects of glycosylation and glycosylation-directed folding. …”
Get full text

Biogenesis of extracellular vesicles in yeast <subtitle>Many questions with few answers</subtitle> by Débora L. Oliveira, Ernesto S. Nakayasu, Luna S. Joffe, Allan J. Guimarães, Tiago J. P. Sobreira, Joshua D. Nosanchuk, Radames J. B. Cordero, Susana Frases, Arturo Casadevall, Igor C. Almeida, Leonardo Nimrichter, Marcio L. Rodrigues

“…These studies suggested that extracellular vesicle release involves components of both conventional and unconventional secretory pathways, although the precise mechanisms required for this process are still unknown. …”
Get full text

IFT20: An Eclectic Regulator of Cellular Processes beyond Intraflagellar Transport by Francesca Finetti, Anna Onnis, Cosima T. Baldari

“…Moreover, its ability to associate with a wide array of interacting partners in a cell-type specific manner has expanded the function of IFT20 to the regulation of intracellular degradative and secretory pathways. In this review, we will present an overview of the multifaceted role of IFT20 in both ciliated and non-ciliated cells.…”
Get full text

Alternative activation of macrophages: immune function and cellular biology. by Varin, A, Gordon, S

“…This review shows how alternative activation of macrophages induces extensive cellular remodelling of phagocytic, endocytic, signaling and secretory pathways which play an important, but unclear role in the pathogenesis of different disease.…”

Protease Profile of Tumor-Associated Mast Cells in Melanoma by Dmitri Atiakshin, Andrey Kostin, Igor Buchwalow, Vera Samoilova, Markus Tiemann

“…In this study, we present data on phenotype and secretory pathways of proteases in MCs in the course of melanoma. …”
Get full text

Vesicular storage, vesicle trafficking, and secretion of leptin and resistin : the similarities, differences and interplays by Ye, Feng, Than, Aung, Zhao, Yanying, Goh, Kian Hong, Chen, Peng

“…Nevertheless, the secretory pathways of these adipokines and their interplays are poorly elucidated. …”
Get full text
Get full text