Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches. by Susanne Przybylla, Jan Stindt, Diana Kleinschrodt, Jan Schulte Am Esch, Dieter Häussinger, Verena Keitel, Sander H Smits, Lutz Schmitt

“…With the exception of radixin, all other interaction partners were integral or membrane-associated proteins including proteins of the early secretory pathway and the bile acyl-CoA synthetase, the second to last, ER-associated enzyme of bile salt synthesis.…”
Get full text

The microneme proteins EtMIC4 and EtMIC5 of Eimeria tenella form a novel, ultra-high molecular mass protein complex that binds target host cells. by Periz, J, Gill, A, Hunt, L, Brown, P, Tomley, F

“…The complex is formed within the parasite secretory pathway and is maintained after secretion onto the surface of the parasite. …”

The Menkes disease ATPase (ATP7A) is internalized via a Rac1-regulated, clathrin- and caveolae-independent pathway. by Cobbold, C, Coventry, J, Ponnambalam, S, Monaco, A

“…The Menkes disease gene encodes a P-type transmembrane ATPase (ATP7A) that translocates cytosolic copper ions across intracellular membranes of compartments along the secretory pathway. ATP7A moves from the trans-Golgi network (TGN) to the cell surface in response to exogenously added copper ions and recycles back to the TGN upon copper removal. …”

Mechanisms of substrate processing during ER-associated protein degradation by Christianson, JC, Jarosch, E, Sommer, TJ

“…Maintaining proteome integrity is essential for long-term viability of all organisms and is overseen by intrinsic quality control mechanisms. The secretory pathway of eukaryotes poses a challenge for such quality assurance as proteins destined for secretion enter the endoplasmic reticulum (ER) and become spatially segregated from the cytosolic machinery responsible for disposal of aberrant (misfolded or otherwise damaged) or superfluous polypeptides. …”

An overexpression screen of Toxoplasma gondii Rab-GTPases reveals distinct transport routes to the micronemes by Kremer, K, Kamin, D, Rittweger, E, Wilkes, J, Flammer, H, Mahler, S, Heng, J, Tonkin, C, Langsley, G, Hell, S, Carruthers, V, Ferguson, D, Meissner, M

“…In order to define the secretory pathway of apicomplexans, we performed an overexpression screen of Rabs in Toxoplasma gondii and identified Rab5A and Rab5C as important regulators of traffic to micronemes and rhoptries. …”

Functional organisation of the Hrd1 ubiquitin ligase complex and its role in ERAD by Schulz, J

“…<p>Endoplasmic reticulum (ER)-associated degradation (ERAD) of misfolded proteins of the secretory pathway is crucial for ER homeostasis and the physiological importance of this mechanism is reflected by more than 60 diseases that have been linked to ERAD to date. …”

Impact of the lectin chaperone calnexin on the stress response, virulence and proteolytic secretome of the fungal pathogen Aspergillus fumigatus. by Margaret V Powers-Fletcher, Kalyani Jambunathan, Jordan L Brewer, Karthik Krishnan, Xizhi Feng, Amit K Galande, David S Askew

“…Calnexin is a membrane-bound lectin chaperone in the endoplasmic reticulum (ER) that is part of a quality control system that promotes the accurate folding of glycoproteins entering the secretory pathway. We have previously shown that ER homeostasis is important for virulence of the human fungal pathogen Aspergillus fumigatus, but the contribution of calnexin has not been explored. …”
Get full text

Analysis of occludin trafficking, demonstrating continuous endocytosis, degradation, recycling and biosynthetic secretory trafficking. by Sarah J Fletcher, Mudassar Iqbal, Sara Jabbari, Dov Stekel, Joshua Z Rappoport

“…Significant co-localisation between occludin and the biosynthetic secretory pathway was demonstrated. Thus, under steady-state conditions occludin undergoes turnover via a continuous cycle of endocytosis, recycling and degradation, with degradation compensated for by biosynthetic exocytic trafficking. …”
Get full text

Perforin rapidly induces plasma membrane phospholipid flip-flop. by Sunil S Metkar, Baikun Wang, Elena Catalan, Gregor Anderluh, Robert J C Gilbert, Julian Pardo, Christopher J Froelich

“…The cytotoxic cell granule secretory pathway is essential for host defense. This pathway is fundamentally a form of intracellular protein delivery where granule proteases (granzymes) from cytotoxic lymphocytes are thought to diffuse through barrel stave pores generated in the plasma membrane of the target cell by the pore forming protein perforin (PFN) and mediate apoptotic as well as additional biological effects. …”
Get full text

A Case of Geroderma Osteodysplasticum Syndrome: Unique Clinical Findings by Maha Alotaibi, Deema Aldhubaiban, Ahmed Alasmari, Leena Alotaibi

“…The encoded protein has a function in the secretory pathway, was identified by terminal kinase-like protein, and thus, it may function in mitosis. …”
Get full text

Selective Protection of an ARF1-GTP Signaling Axis by a Bacterial Scaffold Induces Bidirectional Trafficking Arrest by Andrey S. Selyunin, Lovett Evan Reddick, Bethany A. Weigele, Neal M. Alto

“…How their activities are coordinated in order to define the successive steps of the secretory pathway and preserve traffic directionality is not well understood in part due to the scarcity of molecular tools that simultaneously target ARF and Rab signaling. …”
Get full text

The short mRNA isoform of the immunoglobulin superfamily, member 1 gene encodes an intracellular glycoprotein. by Ying Wang, Emilie Brûlé, Tanya Silander, Beata Bak, Sjoerd D Joustra, Daniel J Bernard

“…Whether the protein plays intracellular functions or is trafficked through the secretory pathway under certain physiologic or pathophysiologic conditions has yet to be determined.…”
Get full text

Extracellular Glycolytic Activities in Root Endophytic <i>Serendipitaceae</i> and Their Regulation by Plant Sugars by Vincenzo De Rocchis, Thomas Roitsch, Philipp Franken

“…Since no conventional signal peptide could be detected in most of the genome sequences encoding the glycolytic enzymes, a non-conventional protein secretory pathway is assumed. The results of the study suggest that root endophytic fungi translocate glycolytic activities into the root, and this process is regulated by the availability of particular plant sugars.…”
Get full text

Codon Bias Can Determine Sorting of a Potassium Channel Protein by Anja J. Engel, Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L. Van Etten, Anna Moroni, Gerhard Thiel

“…By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.…”
Get full text

Towards a unified biological hypothesis for the BDNF Val66Met-associated memory deficits in humans: a model of impaired dendritic mRNA trafficking by Gabriele eBaj, Davide eCarlino, Lucia eGardossi, Enrico eTongiorgi

“…We discuss the clinical evidence of the association between Val66Met and memory deficits, as well as the molecular mechanisms involved including the reduced transport of BDNF mRNA to the dendrites as well as the reduced processing and secretion of BDNF protein through the regulated secretory pathway.…”
Get full text

A new, long-wavelength borondipyrromethene sphingosine for studying sphingolipid dynamics in live cells[S] by Raehyun Kim, Kaiyan Lou, Mary L. Kraft

“…Visualization of BODIPY 540 fluorescence in parallel with GFP-labeled organelle-specific proteins showed the BODIPY 540 sphingosine metabolites were transported through the secretory pathway and were transiently located within lysosomes, mitochondria, and the nucleus. …”
Get full text

Engineering Approaches in Plant Molecular Farming for Global Health by Advaita Acarya Singh, Priyen Pillay, Tsepo Lebiletsa Tsekoa

“…The plant system offers a faster alternative, which is a potentially more cost-effective and scalable platform for the mass production of highly complex protein vaccines, owing to the high degree of similarity between the plant and mammalian secretory pathway. Here, we identify and address bottlenecks in the use of plants for vaccine manufacturing and discuss engineering approaches that demonstrate both the utility and versatility of the plant production system as a viable biomanufacturing platform for global health. …”
Get full text

Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation by Zinia D’Souza, Farhana Taher Sumya, Amrita Khakurel, Vladimir Lupashin

“…The Golgi is the central organelle of the secretory pathway and it houses the majority of the glycosylation machinery, which includes glycosylation enzymes and sugar transporters. …”
Get full text

Pancreatic beta cell ER export in health and diabetes by Cesar Barrabi, Kezhong Zhang, Ming Liu, Xuequn Chen

“…In the secretory pathway of the pancreatic beta cell, proinsulin and other secretory granule proteins are first produced in the endoplasmic reticulum (ER). …”
Get full text

AMPA receptors commandeer an ancient cargo exporter for use as an auxiliary subunit for signaling. by Nadine Harmel, Barbara Cokic, Gerd Zolles, Henrike Berkefeld, Veronika Mauric, Bernd Fakler, Valentin Stein, Nikolaj Klöcker

“…Interaction with GluA subunits breaks with this ancestral role of CNIH-2 confined to the early secretory pathway. While still taking advantage of being exported preferentially from the ER, GluAs recruit CNIH-2 to the cell surface. …”
Get full text