Activation-induced colocalisation of SCAMP5 with IFNα in human plasmacytoid dendritic cells by Timothy R D J Radstake, Marianne Boes, Emmerik F A Leijten, Juliëtte N Pouw, Michel A M Olde Nordkamp, Tom G O'Toole

“…Recent work in transfected HEK cells connects SCAMP5 to the type I IFN secretory pathway. To further study the role of SCAMP5 in IFNα secretion by pDCs, we focused on the subcellular distribution of SCAMP5 in human pDCs freshly isolated from peripheral blood.Methods We measured SCAMP5 expression by flow cytometry in peripheral blood mononuclear cells of healthy subjects (n=8). …”
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Galectins use N-glycans of FGFs to capture growth factors at the cell surface and fine-tune their signaling by Aleksandra Gedaj, Dominika Zukowska, Natalia Porebska, Marta Pozniak, Mateusz Krzyscik, Aleksandra Czyrek, Daniel Krowarsch, Malgorzata Zakrzewska, Jacek Otlewski, Lukasz Opalinski

“…Most of FGFs are released by cells using the conventional secretory pathway and are N-glycosylated, yet the role of FGFs glycosylation is largely unknown. …”
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Assay for dual cargo sorting into endoplasmic reticulum exit sites imaged by 3D Super-resolution Confocal Live Imaging Microscopy (SCLIM). by Sofia Rodriguez-Gallardo, Kazuo Kurokawa, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Ana Maria Perez-Linero, Auxiliadora Aguilera-Romero, Sergio Lopez, Miho Waga, Akihiko Nakano, Manuel Muñiz

“…Understanding how in eukaryotic cells thousands of proteins are sorted from each other through the secretory pathway and delivered to their correct destinations is a central issue of cell biology. …”
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Penta-EF-Hand Protein Peflin Is a Negative Regulator of ER-To-Golgi Transport. by Mariah Rayl, Mishana Truitt, Aaron Held, John Sargeant, Kevin Thorsen, Jesse C Hay

“…Luminal calcium regulates vesicle transport early in the secretory pathway. In ER-to-Golgi transport, depletion of luminal calcium leads to significantly reduced transport and a buildup of budding and newly budded COPII vesicles and vesicle proteins. …”
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Inhibition of the proprotein convertases represses the invasiveness of human primary melanoma cells with altered p53, CDKN2A and N-Ras genes. by Claude Lalou, Nathalie Scamuffa, Samia Mourah, Francois Plassa, Marie-Pierre Podgorniak, Nadem Soufir, Nicolas Dumaz, Fabien Calvo, Nicole Basset-Seguin, Abdel-Majid Khatib

“…Analysis of M10 cells and their gastric and lymph node derived metastatic cells revealed the presence of all the PCs found in the secretory pathway. Expression of the general PCs inhibitor alpha1-PDX in these cells in a stable manner (M10/PDX) had no effect on the mRNA expression levels of these PCs. …”
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XBP-1 Remodels Lipid Metabolism to Extend Longevity by Soudabeh Imanikia, Ming Sheng, Cecilia Castro, Julian L. Griffin, Rebecca C. Taylor

“…Summary: The endoplasmic reticulum unfolded protein response (UPRER) is a cellular stress response that maintains homeostasis within the secretory pathway, regulates glucose and lipid metabolism, and influences longevity. …”
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Exploring the eukaryotic Yip and REEP/Yop superfamily of membrane-shaping adapter proteins (MSAPs): A cacophony or harmony of structure and function? by Timothy Angelotti

“…Polytopic cargo proteins are synthesized and exported along the secretory pathway from the endoplasmic reticulum (ER), through the Golgi apparatus, with eventual insertion into the plasma membrane (PM). …”
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Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells by Nicholas J. Lennemann, Azia S. Evans, Carolyn B. Coyne

“…To define the dynamic process of enterovirus membrane remodeling of major secretory pathway organelles, we have developed plasmid-based reporter systems that utilize viral protease-dependent release of a nuclear-localized fluorescent protein from the endoplasmic reticulum (ER) membrane during infection, while retaining organelle-specific fluorescent protein markers such as the ER and Golgi. …”
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Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda. by Maren Bleckmann, Markus H-Y Fritz, Sabin Bhuju, Michael Jarek, Margitta Schürig, Robert Geffers, Vladimir Benes, Hüseyin Besir, Joop van den Heuvel

“…Nevertheless, expression of some target proteins--especially those entering the secretory pathway--provides a severe challenge for the baculovirus infected insect cells, due to the reorganisation of intracellular compounds upon viral infection. …”
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Regulation of myeloid and lymphoid cell development by O-glycans on Notch by Pamela Stanley, Ankit Tanwar

“…Additional sugars are added in the Golgi compartment during passage through the secretory pathway to the plasma membrane. Of particular significance for Notch signaling is the addition of GlcNAc to O-fucose on an EGF repeat by the Fringe GlcNAc-transferases LFNG, MFNG or RFNG. …”
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Discriminatory Role of Detergent-Resistant Membranes in the Dimerization and Endocytosis of Prostate-Specific Membrane Antigen. by Sonja Schmidt, Birthe Gericke, Giulio Fracasso, Dunia Ramarli, Marco Colombatti, Hassan Y Naim

“…PSMA associates with different types of detergent-resistant membranes (DRMs) along the secretory pathway. Its mature form is mainly insoluble in Lubrol WX, but does not associate with Triton X-100-DRMs. …”
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Functional analysis of a breast cancer-associated mutation in the intracellular domain of the metalloprotease ADAM12. by Dorte Stautz, Ulla M Wewer, Marie Kveiborg

“…Three breast cancer-associated somatic mutations were previously identified in ADAM12, and two of these, one in the metalloprotease domain and another in the disintegrin domain, were investigated and found to result in protein misfolding, retention in the secretory pathway, and failure of zymogen maturation. The third mutation, p.L792F in the ADAM12 cytoplasmic tail, was not investigated, but is potentially significant given its location within a di-leucine motif, which is recognized as a potential cellular trafficking signal. …”
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Vegetative Insecticidal Protein Vip3Aa Is Transported via Membrane Vesicles in <i>Bacillus thuringiensis</i> BMB171 by Yizhuo Zhang, Xuelian Li, Hongwei Tian, Baoju An, Bing Yan, Jun Cai

“…This work demonstrates that Vip3Aa is not secreted by the General Secretion (Sec) System. To reveal the secretory pathway of Vip3A, we purified the membrane vesicles (MVs) of <i>B. thuringiensis</i> BMB171 and observed by TEM. …”
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Autophagy-Dependent Secretion: Crosstalk between Autophagy and Exosome Biogenesis by Ekaterina Zubkova, Alexander Kalinin, Anastasya Bolotskaya, Irina Beloglazova, Mikhail Menshikov

“…Secretory authophagy, initiated by various stress stimuli, prompts the selective release of proteins implicated in inflammation, including leaderless proteins that bypass the conventional endoplasmic reticulum–Golgi secretory pathway. This reflects the significant impact of stress-induced autophagy on cellular secretion profiles, including the modulation of exosome release. …”
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Proprotein convertase 1/3 (PC1/3) in the rat alveolar macrophage cell line NR8383: localization, trafficking and effects on cytokine secretion. by Hugo Gagnon, Sarah Refaie, Sandra Gagnon, Roxane Desjardins, Michel Salzet, Robert Day

“…The proprotein convertase 1/3 (PC1/3) is an important post-translational processing enzyme for the activation of precursor proteins within the regulated secretory pathway. Well characterized for its role in the neural and endocrine systems, we recently reported an unconventional role of PC1/3 as a modulator of the Toll-like receptor innate immune response. …”
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Intracellular events in the assembly of chylomicrons in rabbit enterocytes by Ian J. Cartwright, Dietmar Plonné, Joan A. Higgins

“…In these cells, the cytosolic TAG also increased to 450 μg/g enterocytes, compared with 12 μg/g enterocytes from chow-fed rabbits, indicating that transfer of TAG from the SER membrane into the secretory pathway can become saturated, so that newly synthesized TAG moves into the cytosol.…”
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Tales of the ER-Golgi Frontier: Drosophila-Centric Considerations on Tango1 Function by Zhi Feng, Ke Yang, José C. Pastor-Pareja, José C. Pastor-Pareja

“…In the secretory pathway, the transfer of cargo from the ER to the Golgi involves dozens of proteins that localize at specific regions of the ER called ER exit sites (ERES), where cargos are concentrated preceding vesicular transport to the Golgi. …”
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The capping domain in RalF regulates effector functions. by Eric Alix, Laurent Chesnel, Brad J Bowzard, Aimee M Tucker, Anna Delprato, Jacqueline Cherfils, David O Wood, Richard A Kahn, Craig R Roy

“…Localization mediated by the capping domain of LpRalF enables the GEF to modulate membrane transport in the secretory pathway, whereas, the capping domain of RpRalF enables this bacterial GEF to modulate actin dynamics occurring near the plasma membrane. …”
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Protein bodies in leaves exchange contents through the endoplasmic reticulum by Reza eSaberianfar, Reza eSaberianfar, Amirali eSattarzadeh, Jussi J. Joensuu, Susanne E. Kohalmi, Rima eMenassa

“…Our results confirm the ER origin of all three fusion-tag-induced PBs. We show that secretory pathway proteins can be sequestered into all types of PBs but with different patterns, and that different fusion tags can target a specific protein to different PBs. …”
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Membrane Curvature Sensing by Amphipathic Helices Is Modulated by the Surrounding Protein Backbone. by Christine M Doucet, Nina Esmery, Maud de Saint-Jean, Bruno Antonny

“…They are found in proteins targeted to distinct curved membranes, mostly in the early secretory pathway. For instance, the ALPS motif of the golgin GMAP210 binds trafficking vesicles, while the ALPS motif of Nup133 targets nuclear pores. …”
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