Novel extracellular and nuclear caspase-1 and inflammasomes propagate inflammation and regulate gene expression: a comprehensive database mining study by Luqiao Wang, Hangfei Fu, Gayani Nanayakkara, Yafeng Li, Ying Shao, Candice Johnson, Jiali Cheng, William Y. Yang, Fan Yang, Muriel Lavallee, Yanjie Xu, Xiaoshu Cheng, Hang Xi, Jonathan Yi, Jun Yu, Eric T. Choi, Hong Wang, Xiaofeng Yang

“…Results We made the following important findings: (1) Caspase-1 substrates and interaction proteins are localized in various intracellular organelles including nucleus and secreted extracellularly; (2) Caspase-1 may get activated in situ in the nucleus in response to intra-nuclear danger signals; (3) Caspase-1 cleaves its substrates in exocytotic secretory pathways including exosomes to propagate inflammation to neighboring and remote cells; (4) Most of caspase-1 substrates are upregulated in coronary artery disease regardless of their subcellular localization but the majority of metabolic diseases cause no significant expression changes in caspase-1 nuclear substrates; and (5) In coronary artery disease, majority of upregulated caspase-1 extracellular substrate-related pathways are involved in induction of inflammation; and in contrast, upregulated caspase-1 nuclear substrate-related pathways are more involved in regulating cell death and chromatin regulation. …”
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Genetic regulation of membrane trafficking, exosome secretion, growth and dense-core granule biogenesis in the Drosophila accessory gland by Redhai, S

“…<p>Many epithelial cell types, particularly those in glands, employ distinct regulated and constitutive secretory pathways to perform their functions. Understanding how membrane trafficking and secretion are coupled to other cellular functions like growth, which is also thought to be linked to secretory and endocytic functions, is fundamental to cell biology and to certain pathologies such as diabetes and cancer. …”

Peroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape by Barbora Salovska, Alexandra Kondelova, Kristyna Pimkova, Zuzana Liblova, Miroslav Pribyl, Ivo Fabrik, Jiri Bartek, Marie Vajrychova, Zdenek Hodny

“…Subsequent SILAC-iodoTMT and secretome analysis after PRDX6 silencing showed the downregulation of PRDX6 in IRIS affected protein secretory pathways, decreased expression of extracellular matrix proteins, and led to unexpected attenuation of senescence-associated secretory phenotype (SASP). …”
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Multiple TLRs elicit alternative NLRP3 inflammasome activation in primary human monocytes independent of RIPK1 kinase activity by Sarah Unterberger, Lisa Mullen, Melanie S. Flint, Sandra Sacre

“…Together these data suggest that the alternative pathway operates independently of RIPK1 kinase activity, downstream of diverse TLRs including TLR4 in primary human monocytes and supports the potential for IL-1β release via GSDMD pores alongside other unconventional secretory pathways.…”
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Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton by Richard Odongo Magwanga, Pu Lu, Joy Nyangasi Kirungu, Hejun Lu, Xingxing Wang, Xiaoyan Cai, Zhongli Zhou, Zhenmei Zhang, Haron Salih, Kunbo Wang, Fang Liu

“…Majority of the LEA genes were involved in secretory pathways. Expression profile analysis indicated that most of the LEA genes were highly expressed in drought tolerant cultivars Gossypium tomentosum as opposed to drought susceptible, G. hirsutum. …”
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Islet Stellate Cells Regulate Insulin Secretion via Wnt5a in Min6 Cells by Wei Xu, Peter M. Jones, Houfa Geng, Rui Li, Xuekui Liu, Yinxia Li, Qian Lv, Ying Liu, Jie Wang, Xiuli Wang, Zilin Sun, Jun Liang

“…Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses were used to observe changes in the β-catenin, receptor tyrosine kinase-like orphan receptor 2 (Ror2), Ca (2+)/calmodulin (CaM)-dependent protein kinase II (CamKII), forkhead box O1 (FoxO1), pancreatic and duodenal homeobox 1 (PDX1), glucose transporter 2 (Glut2), insulin, and Cask mRNA and protein levels in the Wnt and insulin secretory pathways. Flow cytometry was used to confirm the intracellular Ca2+ concentration in Min6 cells. …”
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Network Pharmacology Integrated Molecular Docking and Dynamics to Elucidate Saffron Compounds Targeting Human COX-2 Protein by Aarif Ali, Amir Bashir Wani, Bashir Ahmad Malla, Jagadeesha Poyya, Nawab John Dar, Fasil Ali, Sheikh Bilal Ahmad, Muneeb U. Rehman, Ahmed Nadeem

“…Compartmentalized Protein–Protein Interaction identified 90 interactions with an average interaction score of 0.62, and the highest localization score of 0.99 found in secretory pathways. The Computed Atlas of Surface Topography of Proteins was used to identify binding pockets and important residues that could serve as drug targets. …”
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Human parainfluenza virus 3 field strains undergo extracellular fusion protein cleavage to activate entry by Kyle Stearns, George Lampe, Rachel Hanan, Tara Marcink, Stefan Niewiesk, Samuel H. Sternberg, Alexander L. Greninger, Matteo Porotto, Anne Moscona

“…It was previously thought that for parainfluenza virus 3, an important cause of respiratory disease and a representative of a group of important pathogens, this cleavage event was mediated by furin in the cell secretory pathways prior to formation of the virions. We show that this is only true for laboratory strain viruses, and that clinical viruses that infect humans utilize extracellular proteases that are only made by a small subset of cells. …”
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