Cell cycle dysregulation in pituitary oncogenesis. by Muşat, M, Vax, V, Borboli, N, Gueorguiev, M, Bonner, S, Korbonits, M, Grossman, AB

“…Very recent findings from our group have established up-regulation of the serine-threonine kinase Akt in pituitary tumours compared to normal pituitary, which may cause phosphorylation of p27 on Thr157 and cytoplasmic retention of p27. …”

Molecular characterisation and pathogenicity of chicken astrovirus isolated from commercial broiler chickens in Malaysia by Abdullahi, Raji Abdullahi

“…The genomic organisation of the three isolates exhibited three open reading frames, ORF-1a, ORF-1b, and ORF-2, that encode for trypsin-like serine protease, RNA-dependent RNA polymerase (RdRp) and capsid protein, respectively. …”
Get full text

Biodegradation of recalcitrant chicken feather wastes by locally isolated heavy metal tolerant Alcaligenes sp. strain AQ05-001 by Yusuf, Ibrahim

“…Crude keratinase produced by strain AQ05-001 displayed relative activity over a temperature ranging from 30-60°C, pH 7-10 and was highly inhibited by serine inhibitor, Tween20, Trixton-100 and sodium dodecyl sulphate. …”
Get full text

Structural implications of Pseudomonas fluorescence strain AMS8 cold-active lipase in organic solvent by Yaacob, Norhayati

“…Cold-active AMS8 lipase was catalytically active at 25 – 45 °C and has two alpha-helix lids, a pentapeptide motif with nucleophilic-serine and repeat-toxin sequence motifs distribution at C-terminus. …”
Get full text

The biosynthesis of intestinal mucoproteins by Allen, A

“…This is considered to consist of a central protein core (rich in serine and threonine, together 32% of the total amino acids) carrying carbohydrate side-branches (Kent and Marsden, 1963). …”

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma by Jianxun Song, Xiang Chen, Ying Wen, Mingzhu Yin, Yan Cheng, Wenjun Yi, Xingcong Ren, Xisha Chen, Kuansong Wang, Shilong Jiang, Hongyin Sun, Xuanling Che, Minghui Zhang, Jiaying He, Mengting Liao, Xiangling Li, Xiaoming Zhou, Jinming Yang

“…Mechanistically, eEF2K directly bound to and inactivated glycogen synthase kinase 3 beta (GSK3β) by phosphorylating it at serine 9 (S9), leading to PD-L1 protein stabilization and upregulation, and subsequently tumor immune evasion. …”
Get full text

On a Cold Night: Transcriptomics of Grapevine Flower Unveils Signal Transduction and Impacted Metabolism by Mélodie Sawicki, Marine Rondeau, Barbara Courteaux, Fanja Rabenoelina, Gea Guerriero, Eric Gomès, Ludivine Soubigou-Taconnat, Sandrine Balzergue, Christophe Clément, Essaïd Ait Barka, Nathalie Vaillant-Gaveau, Cédric Jacquard

“…We also identified some mechanisms not yet known to be involved in the response to cold stress, i.e., (1) the up-regulation of genes encoding G-type lectin S-receptor-like serine threonine-protein kinase, pathogen recognition receptor (PRR5), or heat-shock factors among others; (2) the down-regulation of Myeloblastosis (MYB)-related transcription factors and the Constans-like zinc finger family; and (3) the down-regulation of some genes encoding Pathogen-Related (PR)-proteins. …”
Get full text

Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas by Nick A. Kuburich, Petra den Hollander, Maria Castaneda, Mika Pietilä, Ximing Tang, Harsh Batra, Francisco Martínez-Peña, Tanvi H. Visal, Tieling Zhou, Breanna R. Demestichas, Ritesh V. Dontula, Jojo Y. Liu, Joanna Joyce Maddela, Reethi S. Padmanabhan, Lan Thi Hanh Phi, Matthew J. Rosolen, Thiru Sabapathy, Dhiraj Kumar, Filippo G. Giancotti, Luke L. Lairson, Maria Gabriela Raso, Rama Soundararajan, Sendurai A. Mani

“…Here, we identify that stabilizing vimentin phosphorylation at serine 56 induces multinucleation, specifically in hybrid E/M cells with stemness properties but not epithelial or mesenchymal cells. …”
Get full text

Integration analysis of metabolome and transcriptome reveals the effect of exogenous supplementation with mixtures of vitamins ADE, zinc, and selenium on follicular growth and gran... by Yajun Guo, Weisen Zhao, Nan Li, Shizhen Dai, Hao Wu, Zhenlong Wu, Shenming Zeng

“…Combined multi-omics analysis revealed that the protein digestion and absorption, ABC transporters, biosynthesis of amino acids, aminoacyl-tRNA biosynthesis, mineral absorption, alanine, aspartate and glutamate metabolism, glycine, serine and threonine metabolism, arginine biosynthesis, and ovarian steroidogenesis were significantly enriched. …”
Get full text

Assessment of Mixed <i>Plasmodium falciparum</i> <i>sera5</i> Infection in Endemic Burkitt Lymphoma: A Case-Control Study in Malawi by Nobuko Arisue, George Chagaluka, Nirianne Marie Q. Palacpac, W. Thomas Johnston, Nora Mutalima, Sally Peprah, Kishor Bhatia, Eric Borgstein, George N. Liomba, Steve Kamiza, Nyengo Mkandawire, Collins Mitambo, James J. Goedert, Elizabeth M. Molyneux, Robert Newton, Toshihiro Horii, Sam M. Mbulaiteye

“…We examined this hypothesis by measuring diversity in <i>Pf-</i>serine repeat antigen-5 (<i>Pfsera5</i>), an antigenic target of blood-stage immunity to malaria, among 200 eBL cases and 140 controls, all <i>Pf</i> polymerase chain reaction (PCR)-positive, in Malawi. …”
Get full text

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii by Xiaoya Shen, Xiaoya Shen, Xiaoya Shen, Yige Chen, Yige Chen, Yige Chen, Liang Jia, Liang Jia, Liang Jia, Wang He, Xiaofeng Chen, Yiwen Chen, Xingrong Xuan, Jiale Li, Jiale Li, Jiale Li, Zhiyi Bai, Zhiyi Bai, Zhiyi Bai, Wenjuan Li, Wenjuan Li, Wenjuan Li

“…We also screened 22 immune-related DEGs in the mantle and visceral mass during the same pearl culture period, including serine/threonine-protein kinase NLK, C-type lectin, and galectin. …”
Get full text

Discovery of a small-molecule NDR1 agonist for prostate cancer therapy by Yang Bai, Yang Bai, Xiuyuan Sui, Xiuyuan Sui, Zuodong Xuan, Zuodong Xuan, Yifan Du, Yifan Du, Meiling Fu, Meiling Fu, Zeyuan Zheng, Zeyuan Zheng, Kunao Yang, Kunao Yang, Chunlan Xu, Chunlan Xu, Yankuo Liu, Yankuo Liu, Bin Liu, Bin Liu, Min Zhong, Min Zhong, Zhengying Zhang, Zhengying Zhang, Jianzhong Zheng, Xiaoyan Hu, Lei Zhang, Huimin Sun, Huimin Sun, Chen Shao, Chen Shao

“…Nuclear Dbf2-related kinase 1 (NDR1), also known as STK38, is a serine/threonine kinase in the NDR/LATS kinase family that plays a critical role in cellular processes, including immunity, inflammation, metastasis, and tumorigenesis. …”
Get full text

Pembrolizumab as a monotherapy or in combination with platinum-based chemotherapy in advanced non-small cell lung cancer with PD-L1 tumor proportion score (TPS) ≥50%: real-world da... by Elizabeth Dudnik, Mor Moskovitz, Yakir Rottenberg, Anastasiya Lobachov, Rinat Mandelboim, Tzippy Shochat, Damien Urban, Mira Wollner, Hovav Nechushtan, Ofer Rotem, Alona Zer, Sameh Daher, Jair Bar, On behalf of the Israel Lung Cancer Group

“…In the real-world setting, no statistically significant differences in long-term outcomes with P vs PCT were observed; a prospective randomized trial addressing the comparative efficacy of P and PCT in different patient subgroups is highly anticipated.List of abbreviations: AE - adverse events; ALK - anaplastic lymphoma kinase gene; ALT - alanine aminotransferase; (a)NSCLC - (advanced) non-small cell lung cancer; AST - aspartate aminotransferase; BRAF - v-Raf murine sarcoma viral oncogene homolog B; BRCA2 - BReast CAncer gene 2; c-Met - tyrosine-protein kinase Met; CTCAE, v. 4.03 - Common Terminology Criteria for Adverse Events, version 4.03; CTLA-4 - cytotoxic T-lymphocyte-associated protein 4; ECOG PS - Eastern Cooperative Oncology Group performance status; EGFR - epidermal growth factor receptor gene; FISH - fluorescent in situ hybridization; HER2 - human epidermal growth factor receptor 2; IC - tumor-infiltrating immune cells; ICI - immune check-point inhibitors; IHC - immunohistochemistry; IQR - interquartile range; irAE - immune related adverse events; ISCORT - Israeli Society for Clinical Oncology and Radiotherapy; KRAS - Kirsten rat sarcoma viral oncogene homolog; (m)TTD -(median) time-to-treatment discontinuation; mo - months; (m)OS - (median) overall survival; (m)PFS - (median) progression-free survival; muts/Mb - mutations per megabase; NA - not specified/not available; NOS - not otherwise specified; NR - not reported/not reached; ORR - objective response rate; P - pembrolizumab; PCR - polymerase chain reaction; PCT - combination of pembrolizumab with platinum-based chemotherapy; PD – progression of disease; PD-1 - programmed cell death-1; PD-L1 - programmed cell death ligand-1; pts - patients; RET - proto-oncogene RET; ROS1 - proto-oncogene tyrosine-protein kinase ROS1; SD - standard deviation; STK11 - serine/threonine kinase 11; TC - tumor cells; TMB - Tumor mutation burden; TPS - tumor proportion score.…”
Get full text

Does the human placenta express the canonical cell entry mediators for SARS-CoV-2? by Roger Pique-Regi, Roberto Romero, Adi L Tarca, Francesca Luca, Yi Xu, Adnan Alazizi, Yaozhu Leng, Chaur-Dong Hsu, Nardhy Gomez-Lopez

“…The novel coronavirus canonically utilizes the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2 for cell entry. Herein, building upon our previous single-cell study (Pique-Regi et al., 2019), another study, and new single-cell/nuclei RNA-sequencing data, we investigated the expression of ACE2 and TMPRSS2 throughout pregnancy in the placenta as well as in third-trimester chorioamniotic membranes. …”
Get full text

Blood gene expression biomarkers of response to anti-TNF drugs in pediatric inflammatory bowel diseases before initiation of treatment by Sara Salvador-Martín, Gianluca Rubbini, Perceval Vellosillo, Paula Zapata-Cobo, Marta Velasco, Laura M. Palomino, Susana Clemente, Oscar Segarra, Ana Moreno-Álvarez, Ana Fernández-Lorenzo, Begoña Pérez-Moneo, Montserrat Montraveta, Cesar Sánchez, Mar Tolín, Inés Loverdos, María José Fobelo, Victor Manuel Navas-López, Lorena Magallares, Ruth García-Romero, Ricardo Torres-Peral, Alejandro Rodríguez, Ferrán Bossacoma, Vicente Merino-Bohórquez, Enrique Salcedo, Rebeca Álvarez, Ana Dopazo, María Sanjurjo-Sáez, Luis A. López-Fernández

“…Genes participating in defense response to Gram-negative bacterium, serine-type endopeptidase activity, and transcriptional misregulation in cancer are good candidates for anticipating the response to anti-TNF drugs in children with IBDs.…”
Get full text

Selective Targeting of Protein Kinase C (PKC)-θ Nuclear Translocation Reduces Mesenchymal Gene Signatures and Reinvigorates Dysfunctional CD8⁺ T Cells in Immunotherapy-R... by Jenny Dunn, Robert D. McCuaig, Abel H. Y. Tan, Wen Juan Tu, Fan Wu, Kylie M. Wagstaff, Anjum Zafar, Sayed Ali, Himanshu Diwakar, Jane E. Dahlstrom, Elaine G. Bean, Jade K. Forwood, Sofiya Tsimbalyuk, Emily M. Cross, Kristine Hardy, Amanda L. Bain, Elizabeth Ahern, Riccardo Dolcetti, Roberta Mazzieri, Desmond Yip, Melissa Eastgate, Laeeq Malik, Peter Milburn, David A. Jans, Sudha Rao

“…Protein kinase C (PKC)-θ is a serine/threonine kinase with both cytoplasmic and nuclear functions. …”
Get full text

Hyperforin-mediated anticancer mechanisms in MDA-MB-231 cell line: insights into apoptotic mediator modulation and caspase activation by Muttiah Barathan, Ahmad Khusairy Zulpa, Kumutha Malar Vellasamy, Zaridatul Aini Ibrahim, See Mee Hoong, Vanitha Mariappan, Gopinath Venkatraman, Jamuna Vadivelu

“…The results suggest that hyperforin may act as a good cell death-inducing agent.ABBREVIATIONS: TNBC: Triple negative breast cancer; DNA: Deoxyribonucleic acid; RNA: Ribonucleic acid; ROS: Reactive oxygen species; 5-FU: Fluorouracil; TRAIL: TNF-related apoptosis-inducing ligand; AKT: Protein kinase B; COX-2: Cyclooxygenase-2; PCR: Polymerase chain reaction; Bax: Bcl-2-associated X protein; BCL-2: B-cell lymphoma 2; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; mRNA: Messenger ribonucleic acid; MCL1: Myeloid-cell leukemia 1; LDH: Lactate dehydrogenase; ELISA: Enzyme-linked immunosorbent assay; MMP: Mitochondrial membrane potential; MCP-1: Monocyte chemoattractant protein-1; ATCC: American Type Culture Collection; RPMI: Roswell Park Memorial Institute; XIAP: X-linked inhibitor of apoptosis protein; FBS: Fetal bovine serum; MCL-1: Myeloid-cell leukemia 1; DMEM: Dulbecco’s Modified Eagle Medium; DMSO: Dimethyl sulfoxide; CASP3: Caspase 3; RPMI: Roswell Park Memorial Institute; DMEM: Dulbecco’s Modified Eagle Medium; FBS: Fetal bovine serum; h: Hour; TNF-α: Tumour necrosis factor alpha; P53: Tumor protein P53; min: Minutes; cps: Count presecond; OD: Optical density; rpm: Revolutions per minute; PI: Propidium iodide; SD: Standard deviation; DDR: Death Domoin Receptor; TRADD: Tumor necrosis factor receptor type 1-associated DEATH domain; HRK: Harakiri, BCL2 Interacting Protein; RIPK2: Receptor-interacting serine/threonine-protein kinase 2; BIK: BCL2 interacting killer; DCF: 2’, 7’ –dichlorofluorescein; DCF-DA: Dichlorofluorescin Diacetate; FADD: Fas Associated Via Death Domain; TNFRSF: Tumor necrosis factor receptor superfamily; FITC: Fluorescein isothiocyanate; DMSO: Dimethyl sulfoxide; CFLAR: CASP8 And FADD Like Apoptosis Regulator; BID: BH3-interacting domain death agonist; BIRC: Baculoviral IAP repeat-containing protein; CIDEA: Cell death activator CIDE-A; CIDEB: Cell Death Inducing DFFA Like Effector B; BrdU: Bromodeoxyuridine; RFU: Relative fluorescence unit; NF-kB: Nuclear Factor kappa-light-chain-enhancer of activated B cells; %: Percentage; µL: microliter; µM: Micromolar; °C: degree Celcius; ng/mL: Nanograms per millimeter; mg/mL: Milligrams per milliliter; µg/mL: Micrograms per milliliter; ΔΨm: Mitochondrial membrane potential; TNFα: Tumour necrosis factor α; Bcl-xL: B-cell lymphoma-extra large; MDR1: Multidrug Resistance Mutation; AIB1: Amplified in breast cancer 1; LTBR: Lymphotoxin Beta Receptor HighlightsUtilization of hyperforin as anticancer agent against triple negative breast cancer.Hyperforin exhibited a lower IC50 compared to taxol in inhibiting growth of MDA-MB-231 cells.Hyperforin shows similar potential anticancer activity as taxol.Hyperforin uses mitochondrial death pathway to induce cell death in MDA-MB-231 cells.…”
Get full text

Expression of Selected Genes and Circulating microRNAs in Patients with Celiac Disease by Elena Maria Domsa, Ioana Berindan-Neagoe, Livia Budisan, Cornelia Braicu, Ioana Para, Alina Ioana Tantau, Olga Hilda Orasan, Lidia Ciobanu, Teodora Atena Pop, Gabriela Adriana Filip, Nicoleta Leach, Vasile Negrean, Daniela Matei, Vasile Andreica

“…The aim of the study is to describe the expression of selected genes (Wnt family member 3, <i>WNT3</i>; Wnt family member 11, <i>WNT11</i>; tumor necrosis factor alpha, <i>TNFα</i>; mitogen-activated protein kinase 1, <i>MAPK1</i>; AKT serine/threonine kinase 3, <i>AKT3</i>; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, <i>PIK3CA</i>; and cyclin <i>D1, CCND1</i>) and miRNAs (miR-192-5p, miR-194-5p, miR-449a and miR-638) in adult patients with CD. …”
Get full text

Effective-component compatibility of Bufei Yishen formula alleviates chronic obstructive pulmonary disease inflammation by regulating GSK3β-mediated NLRP3 inflammasome activation by Yanqin Qin, Jiena Zhai, Jingfan Yang, Haibo Li, Yange Tian, Xuefang Liu, Peng Zhao, Jiansheng Li

“…Moreover, ECC-BYF suppressed the activation of the NLRP3 inflammasome by increasing the phosphorylation at serine 9 and decreasing the phosphorylation at tyrosine 216 of GSK3β, followed by the inhibition of IL-1β secretion in macrophages. …”
Get full text

In silico study of novel niclosamide derivatives, SARS-CoV-2 nonstructural proteins catalytic residue-targeting small molecules drug candidates by Bashir Lawal, Sheng-Kuang Tsai, Alexander T.H. Wu, Hsu-Shan Huang

“…In the present study, we reported eight (SCOV-L-02, SCOV-L-09, SCOV-L-10, SCOV-L-11, SCOV-L-15, SCOV-L-18, SCOV-L-22, and SCOV-L-23) novel structurally related small-molecule derivatives of niclosamide (SCOV-L series) for their targeting potential against angiotensin-converting enzyme-2 (ACE2), type II transmembrane serine protease (TMPRSS2), and SARS-COV-2 nonstructural proteins (NSPs) including NSP5 (3CLpro), NSP3 (PLpro), and RdRp. …”
Get full text