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    Novel enzyme-polymer conjugates for biotechnological applications by Oscar Romero, Cintia W. Rivero, Jose M. Guisan, Jose M. Palomo

    Published 2013-02-01
    “…First we focused on the protein engineering of the Geobacillus thermocatenulatus lipase 2 (BTL2) to replace the two cysteines (Cys65, Cys296) in the wild type enzyme (BTL-WT) by two serines. Then, by similar mode, a unique cysteine was introduced in the lid area of the protein. …”
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