Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding. von Zhou, M, Morgner, N, Politis, A, Isaacson, S, Matak-Vinković, D, Murata, T, Bernal, R, Stock, D, Robinson, C

“… Mass spectra reveal subunit stoichiometries and the identity of tightly bound lipids within the membrane rotors.  …”

Quantifying protein-protein interactions by molecular counting with mass photometry von Soltermann, F, Foley, E, Pagnoni, V, Galpin, M, Benesch, J, Kukura, P, Struwe, W

“… These measurements enable us to quantify binding affinities over four orders of magnitude at equilibrium for both simple and complex stoichiometries within minutes, as well as to determine the associated kinetics.  …”

Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers von Foley, EDB, Kushwah, MS, Young, G, Kukura, P

“… State-of-the-art single-molecule approaches rely largely on the addition of fluorescent labels, which complicates the quantification of the involved stoichiometries and dynamics because of low temporal resolution and the inherent limitations associated with labeling efficiency, photoblinking and photobleaching.  …”

Evaluation of complexes of DNA duplexes and novel benzoxazoles or benzimidazoles by electrospray ionization mass spectrometry. von Oehlers, L, Mazzitelli, C, Brodbelt, J, Rodriguez, M, Kerwin, S

“… Electrospray ionization mass spectrometry is used to compare the metal ion binding and metal-mediated DNA binding of benzoxazole (1, 2, 3, 4) and benzimidazole (5) compounds and to elucidate the putative binding modes and stoichiometries. The observed metal versus non-metal-mediated DNA binding, as well as the specificity of DNA binding, is correlated with the biological activities of the analogs.  …”

Quaternary dynamics and plasticity underlie small heat shock protein chaperone function. von Stengel, F, Baldwin, A, Painter, A, Jaya, N, Basha, E, Kay, L, Vierling, E, Robinson, C, Benesch, J

“… We observe over 300 different stoichiometries of interaction, demonstrating that an ensemble of structures underlies the protection these chaperones confer to unfolding clients.  …”

Dissecting heterogeneous molecular chaperone complexes using a mass spectrum deconvolution approach. von Stengel, F, Baldwin, A, Bush, M, Hilton, G, Lioe, H, Basha, E, Jaya, N, Vierling, E, Benesch, J

“… We present a nanoelectrospray mass spectrometry analysis algorithm for estimating the distribution of stoichiometries comprising a polydisperse ensemble of oligomers.  …”

An oligomeric signaling platform formed by the Toll-like receptor signal transducers MyD88 and IRAK-4. von Motshwene, P, Moncrieffe, M, Grossmann, J, Kao, C, Ayaluru, M, Sandercock, A, Robinson, C, Latz, E, Gay, N

“… In this work, we show that the death domains of human MyD88 and IRAK-4 assemble into closed complexes having unusual stoichiometries of 7:4 and 8:4, the Myddosome. Formation of the Myddosome is likely to be a key event for TLR4 signaling in vivo as we show here that pathway activation requires that the receptors cluster into lipid rafts.  …”

In vivo single-molecule imaging of bacterial DNA replication, transcription, and repair. von Stracy, M, Uphoff, S, Garza de Leon, F, Kapanidis, A

“… We discuss how single-molecule tracking and quantitative super-resolution microscopy can be adapted to extract DNA binding kinetics, spatial distributions, and copy numbers of proteins, as well as stoichiometries of protein complexes. We highlight experiments which have exploited these techniques to answer important questions in the field of bacterial gene regulation and transcription, as well as chromosome replication, organisation and repair.  …”

Understanding protein mobility in bacteria by tracking single molecules von Kapanidis, A, Uphoff, S, Stracy, M

“… We also discuss studies that provide estimates of the time a diffusing protein takes to locate a target site, as well as studies that examined the stoichiometries of diffusing species, the effect of stable and weak interactions on diffusion, and the constraints of large macromolecular structures on the ability of proteins and their complexes to access the entire cytoplasm. …”

Quaternary dynamics and plasticity underlie small heat shock protein chaperone function von Stengel, F, Baldwin, A, Painter, A, Robinson, C, Benesch, J, al., E

“… We observe over 300 different stoichiometries of interaction, demonstrating that an ensemble of structures underlies the protection these chaperones confer to unfolding clients.  …”

SYNTHESIS AND PROPERTIES OF THE LAYERED OXYCARBONATES SR2-XBAXCUO2(CO3) von Armstrong, A, Obhi, H, Edwards, P

“… Both effects are attributed to the presence of additional oxygen in the structure of compounds at these stoichiometries, analogous to that found earlier for (Ba, Sr)2CuO3+δ materials. © 1993 Academic Press.  …”

Synthetic, structural and reaction chemistry of N-heterocyclic germylene and stannylene compounds featuring N-boryl substituents von Kristinsdóttir, L, Oldroyd, N, Grabiner, R, Knights, A, Heilmann, A, Protchenko, A, Niu, H, Kolychev, E, Campos, J, Hicks, J, Christensen, K, Aldridge, S

“… The reactivity of the germylene systems towards oxygen atom transfer agents has been examined, with 2 : 1 reaction stoichiometries being observed for both Me3NO and pyridine N-oxide, leading to the formation of products thought to be derived from the activation of C-H bonds by a transient first-formed germanone. …”

Optoelectronic properties of tin-lead halide perovskites von Savill, KJ, Ulatowski, AM, Herz, LM

“… However, tin-rich stoichiometries still suffer from tin oxidation and vacancy formation which often obscures the fundamentally achievable performance, causing high background hole densities, accelerating charge-carrier recombination, lowering charge-carrier mobilities, and blue-shifting absorption onsets through the Burstein–Moss effect.  …”

Interaction of two complementary fragments of the bovine spinal cord myelin basic protein with phosphatidylglycerol bilayers, studied by 2H and 31P NMR spectroscopy. von Hayer-Hartl, M, Brophy, P, Marsh, D, Watts, A

“… The relative gradients of the dependence on protein/lipid ratio of both these parameters decrease with the size of the protein fragment and correlate reasonably well with both the net charge on the protein and the lipid binding stoichiometries in the absence of salt. The results are therefore consistent with a model in which the perturbation of the quadrupole splittings either is determined by the net surface potential or is constant for the different protein fragments.  …”

Subunit exchange of multimeric protein complexes. Real-time monitoring of subunit exchange between small heat shock proteins by using electrospray mass spectrometry von Sobott, F, Benesch, J, Vierling, E, Robinson, C

“… A comparison with spectra obtained from statistical modeling demonstrates that after equilibration the distribution of these heterocomplexes is governed by the starting ratio of the two components rather than an inherent preference for certain stoichiometries. This finding suggests that the two different sHSP subunits interact in a very similar manner.  …”

Kinetic Study of Lithiation-Induced Phase Transitions in Amorphous Germanium Thin Films von Miao, Jinghui, Wang, Baoming, Thompson, Carl Vernette

“… In this regime, three phases, two amorphous phases with different stoichiometries and the crystalline phase, can co-exist.  …”
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Protein purification by using immobilized metal ions affinity zeolite adsorbent von Su Najwa, Mohamed

“… This result proves that adsorption capacity of Bovine Serum Albumin depends on the types of adsorbent used, which means the adsorption stoichiometries depends on physical characteristic of adsorbent that could increase and reduce the adsorption capacity of the protein as well as the pH of protein.  …”
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Charge discharge studies of LiNii.xCoxFey02 cathode materials / Nur Fatin Farhanah Nazarudin von Nazarudin, Nur Fatin Farhanah

“… Results show charge-discharge time for the three cathode materials from the first cycle to third cycle differs for different stoichiometries. …”
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Mass spectrometry: from plasma proteins to mitochondrial membranes von Robinson, C

“… During these investigations, we observed many assemblies and intermediates with different subunit stoichiometries, and were keen to ascertain whether or not their overall topology was preserved in the mass spectrometer.  …”

Subunit exchange of multimeric protein complexes. Real-time monitoring of subunit exchange between small heat shock proteins by using electrospray mass spectrometry. von Sobott, F, Benesch, J, Vierling, E, Robinson, C

“… A comparison with spectra obtained from statistical modeling demonstrates that after equilibration the distribution of these heterocomplexes is governed by the starting ratio of the two components rather than an inherent preference for certain stoichiometries. This finding suggests that the two different sHSP subunits interact in a very similar manner.  …”