Functional and structural proteomics of glycoproteins by Owens, R, Nettleship, J

Structural proteomics: High-throughput methods by Owens, R

The impact of protein characterization in structural proteomics. by Geerlof, A, Brown, J, Coutard, B, Egloff, M, Enguita, F, Fogg, M, Gilbert, R, Groves, MR, Haouz, A, Nettleship, J, Nordlund, P, Owens, R, Ruff, M, Sainsbury, S, Svergun, D, Wilmanns, M

“…Protein characterization plays a role in two key aspects of structural proteomics. The first is the quality assessment of the produced protein preparations. …”

Eukaryotic expression: developments for structural proteomics. by Aricescu, A, Assenberg, R, Bill, R, Busso, D, Chang, V, Davis, S, Dubrovsky, A, Gustafsson, L, Hedfalk, K, Heinemann, U, Jones, I, Ksiazek, D, Lang, C, Maskos, K, Messerschmidt, A, Macieira, S, Peleg, Y, Perrakis, A, Poterszman, A, Schneider, G, Sixma, T, Sussman, J, Sutton, G, Tarboureich, N, Zeev-Ben-Mordehai, T

“…Groups in the Structural Proteomics In Europe (SPINE) consortium have developed and implemented high-throughput (HTP) methodologies for cloning, expression screening and protein production in eukaryotic systems. …”

SPINE: Structural Proteomics in Europe - The best of both worlds by Wilson, K, Daenke, S, Stuart, D, Jones, E

Native mass spectrometry: towards high-throughput structural proteomics. by Kondrat, F, Struwe, W, Benesch, J

“…Native mass spectrometry (MS) has become a sensitive method for structural proteomics, allowing practitioners to gain insight into protein self-assembly, including stoichiometry and three-dimensional architecture, as well as complementary thermodynamic and kinetic aspects. …”

The genome and structural proteome of an ocean siphovirus: a new window into the cyanobacterial 'mobilome' by Sullivan, Matthew B., Krastins, Bryan, Hughes, Jennifer L., Hughes, Jennifer L., Kelly, Libusha, Chase, Michael, Sarracino, David, Chisholm, Sallie (Penny)

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Application of high-throughput technologies to a structural proteomics-type analysis of Bacillus anthracis. by Au, K, Berrow, N, Blagova, E, Boucher, I, Boyle, M, Brannigan, J, Carter, L, Dierks, T, Folkers, G, Grenha, R, Harlos, K, Kaptein, R, Kalliomaa, A, Levdikov, V, Meier, C, Milioti, N, Moroz, O, Müller, A, Owens, R, Rzechorzek, N, Sainsbury, S, Stuart, D, Walter, T, Waterman, D, Wilkinson, A

“…A collaborative project between two Structural Proteomics In Europe (SPINE) partner laboratories, York and Oxford, aimed at high-throughput (HTP) structure determination of proteins from Bacillus anthracis, the aetiological agent of anthrax and a biomedically important target, is described. …”

Structural proteomics : elucidating in vivo structural dynamics of integral membrane proteins by hydroxyl radical footprinting by Zhu, Yi

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A heuristic derived from analysis of the ion channel structural proteome permits the rapid identification of hydrophobic gates by Rao, S, Klesse, G, Stansfeld, PJ, Tucker, SJ, Sansom, M

Recombinant protein expression and solubility screening in Escherichia coli: a comparative study. by Berrow, N, Büssow, K, Coutard, B, Diprose, J, Ekberg, M, Folkers, G, Levy, N, Lieu, V, Owens, R, Peleg, Y, Pinaglia, C, Quevillon-Cheruel, S, Salim, L, Scheich, C, Vincentelli, R, Busso, D

“…Producing soluble proteins in Escherichia coli is still a major bottleneck for structural proteomics. Therefore, screening for soluble expression on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. …”

Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis (BA4489). by Meier, C, Carter, L, Winter, G, Owens, R, Stuart, D, Esnouf, R

“…The Oxford Protein Production Facility has been targeting proteins from B. anthracis in order to develop high-throughput technologies within the Structural Proteomics in Europe project. As part of this work, the structure of 5-formyltetrahydrofolate cyclo-ligase (BA4489) has been determined by X-ray crystallography to 1.6 A resolution. …”

High-throughput sample handling and data collection at synchrotrons: embedding the ESRF into the high-throughput gene-to-structure pipeline. by Beteva, A, Cipriani, F, Cusack, S, Delageniere, S, Gabadinho, J, Gordon, E, Guijarro, M, Hall, DR, Larsen, S, Launer, L, Lavault, C, Leonard, G, Mairs, T, McCarthy, A, McCarthy, J, Meyer, J, Mitchell, E, Monaco, S, Nurizzo, D, Pernot, P, Pieritz, R, Ravelli, R, Rey, V, Shepard, W, Spruce, D

“…An automatic data-collection system has been implemented and installed on seven insertion-device beamlines and a bending-magnet beamline at the ESRF (European Synchrotron Radiation Facility) as part of the SPINE (Structural Proteomics In Europe) development of an automated structure-determination pipeline. …”

SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects. by Berry, I, Dym, O, Esnouf, R, Harlos, K, Meged, R, Perrakis, A, Sussman, J, Walter, T, Wilson, J, Messerschmidt, A

“…This paper reviews the developments in high-throughput and nanolitre-scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe (SPINE) project since the project's inception in October 2002. …”

SPINE workshop on automated X-ray analysis: a progress report. by Bahar, M, Ballard, C, Cohen, S, Cowtan, K, Dodson, E, Emsley, P, Esnouf, R, Keegan, R, Lamzin, V, Langer, G, Levdikov, V, Long, F, Meier, C, Muller, A, Murshudov, G, Perrakis, A, Siebold, C, Stein, N, Turkenburg, MG, Vagin, A, Winn, M, Winter, G, Wilson, K

“…The Structural Proteomics In Europe (SPINE) consortium contained a workpackage to address the automated X-ray analysis of macromolecules. …”

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value. by Banci, L, Bertini, I, Cusack, S, de Jong, R, Heinemann, U, Jones, E, Kozielski, F, Maskos, K, Messerschmidt, A, Owens, R, Perrakis, A, Poterszman, A, Schneider, G, Siebold, C, Silman, I, Sixma, T, Stewart-Jones, G, Sussman, J, Thierry, J, Moras, D

“…The EC 'Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer (kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. …”

Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens by Fogg, MJ, Alzari, P, Bahar, M, Bertini, I, Betton, JM, Burmeister, WP, Cambillau, C, Canard, B, Corrondo, MA, Coll, M, Daenke, S, Dym, O, Egloff, MP, Enguita, FJ, Geerlof, A, Haouz, A, Jones, TA, Ma, Q, Manicka, SN, Migliardi, M, Nordlund, P, Owens, RJ, Peleg, Y, Schneider, G, Schnell, R, Stuart, DI, Tarbouriech, N, Unge, T, Wilkinson, AJ, Wilmanns, M, Wilson, KS, Zimhony, O, Grimes, JM

“… <p style="text-align:justify;"> The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. …”

Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens. by Fogg, M, Alzari, P, Bahar, M, Bertini, I, Betton, J, Burmeister, W, Cambillau, C, Canard, B, Corrondo, M, Carrondo, M, Coll, M, Daenke, S, Dym, O, Egloff, M, Enguita, F, Geerlof, A, Haouz, A, Jones, T, Ma, Q, Manicka, SN, Migliardi, M, Nordlund, P, Owens, R, Peleg, Y, Schneider, G, Schnell, R, Stuart, D, Tarbouriech, N, Unge, T, Wilkinson, A, Wilmanns, M, Wilson, K, Zimhony, O, Grimes, J

“…The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. …”

Honing the in silico toolkit for detecting protein disorder. by Esnouf, R, Hamer, R, Sussman, J, Silman, I, Trudgian, D, Yang, Z, Prilusky, J

“…Such design criteria have become integral to the construct-design strategies of laboratories within the Structural Proteomics In Europe (SPINE) consortium. This paper assesses the current state of the art in disorder prediction in terms of prediction reliability and considers how best to use these methods to guide construct design. …”

SPINE workshop on automated X-ray analysis: a progress report by Bahar, M, Ballard, C, Cohen, SX, Cowtan, KD, Dodson, EJ, Emsley, P, Esnouf, RM, Keegan, R, Lamzin, V, Langer, G, Levdikov, V, Long, F, Meier, C, Muller, A, Murshudov, GN, Perrakis, A, Siebold, C, Stein, N, Turkenburg, MG, Vagin, AA, Winn, M, Winter, G, Wilson, KS

“… <p style="text-align:justify;"> The Structural Proteomics In Europe (SPINE) consortium contained a workpackage to address the automated X-ray analysis of macromolecules. …”