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601
Assessing the Orthogonality of Phage-Encoded RNA Polymerases for Tailored Synthetic Biology Applications in <i>Pseudomonas</i> Species
Published 2023-04-01“…The phage T7 RNA polymerase (RNAP) and lysozyme form the basis of the widely used pET expression system for recombinant expression in the biotechnology field and as a tool in microbial synthetic biology. Attempts to transfer this genetic circuitry from <i>Escherichia coli</i> to non-model bacterial organisms with high potential have been restricted by the cytotoxicity of the T7 RNAP in the receiving hosts. …”
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602
Evaluation of existing guidelines for their adequacy for the microbial characterisation and environmental risk assessment of microorganisms obtained through synthetic biology
Published 2020-10-01Subjects: “…synthetic biology…”
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603
Synthetic biology is essential to unlock commercial biofuel production through hyper lipid-producing microalgae: a review
Published 2021-01-01“…This development focuses on the use of synthetic biology to produce genetically modified industrial microalgal strains that hyper-accumulate neutral lipids. …”
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604
Synthetic Biology Tool Development Advances Predictable Gene Expression in the Metabolically Versatile Soil Bacterium Rhodopseudomonas palustris
Published 2022-03-01Subjects: “…synthetic biology tools…”
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605
Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening.
Published 2017-01-01“…Successfully employed in other synthetic biology applications, we demonstrate its advantageous use to engineer a biocatalyst. …”
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606
Advancing the scale of synthetic biology via cross-species transfer of cellular functions enabled by iModulon engraftment
Published 2024-03-01“…Combining big data analytics and laboratory evolution thus enhances the level of understanding of systems biology, and synthetic biology for strain design and development.…”
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607
Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology
Published 2024-01-01“…In this study, we have adapted and implemented an Agrobacterium tumefaciens-mediated transformation protocol for A. vadensis for the first time, and applied the FungalBraid system to genetically modify this species by means of synthetic biology. As proof of concept, we have successfully complemented and fluorescently labelled a uridine auxotrophic A. vadensis pyrA- strain and generated A. vadensis mutants carrying the Penicillium expansum-based expression cassette for the heterologous production of the antifungal protein PeAfpA from P. expansum. …”
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608
The effect of metabolic stress on genome stability of a synthetic biology chassis Escherichia coli K12 strain
Published 2018-01-01Subjects: Get full text
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609
Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology.
Published 2012-01-01“…The simplicity of targeted integration in the ku70 deletion strain will further support protein production strain generation and synthetic biology using P. pastoris strains as platform hosts.…”
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610
Vanadium oxide 3- methoxy salen, a synthetic biologically active complex against cancerous cell lines
Published 2023-02-01Get full text
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611
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A Novel Hydroxylation Step in the Taxane Biosynthetic Pathway: A New Approach to Paclitaxel Production by Synthetic Biology
Published 2020-05-01“…The identification of this hydroxylase will contribute to the development of alternative sustainable paclitaxel production systems using synthetic biology techniques.…”
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613
The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range
Published 2021-03-01“…The review also highlights the last development in metabolic modeling and engineering and in synthetic biology to direct surfactin biosynthesis but also to generate novel derivatives. …”
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614
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How Synthetic Biology and Metabolic Engineering Can Boost the Generation of Artificial Blood Using Microbial Production Hosts
Published 2018-11-01Subjects: “…synthetic biology…”
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616
Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
Published 2024-03-01“…Here, we develop a modular synthetic biology toolkit for Aspergillus oryzae, an edible fungus used in fermented foods, protein production, and meat alternatives. …”
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617
Location of cobalt impurities in the surface oxide of stainless steel 316L and metal release in synthetic biological fluids
Published 2022-03-01“…Time-of-flight secondary ion mass spectrometry (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma mass spectrometry (ICPMS) investigated the location of Co in the surface oxide and extent of release along with other metals (iron, chromium, nickel, and manganese) into synthetic biological fluids (gastric fluid, pH 1.5; lysosomal fluid, pH 4.5; phosphate buffered saline-PBS, pH 7.4). …”
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618
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619
Metabolic Engineering Strategies in Diatoms Reveal Unique Phenotypes and Genetic Configurations With Implications for Algal Genetics and Synthetic Biology
Published 2020-06-01“…Using high-throughput phenotyping we confirmed the suitability of episomes for synthetic biology applications and identified superior geraniol-yielding strains following random integration. …”
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620