Protocol to generate an in vitro model to study vascular calcification using human endothelial and smooth muscle cells by Elisa Persiani, Elisa Ceccherini, Ilaria Gisone, Antonella Cecchettini, Federico Vozzi

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells by Yukiko Tando, Yasuhisa Matsui

“…Here, we describe steps for detecting DNA methylation changes in mouse germ cells isolated from both embryonic and spermatogenic stages after maternal exposure to a chemical compound.For complete details on the use and execution of this protocol, please refer to Tando et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Rapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein by Aditi A. Ghuge, Reuben A. Anderson, Susanne Gottfried, Clément Daube, Siaosi M.B.M.J. Koloamatangi, Anja H. Schiemann, Evelyn Sattlegger

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Protocol for analyzing energy metabolic pathway dependency in human liver cancer cell lines by Sk Ramiz Islam, Sebabrata Maity, Oishee Chakrabarti, Soumen Kanti Manna

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Protocol for gene annotation, prediction, and validation of genomic gene expansion by Quanwei Zhang, Zhengdong D. Zhang

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model by Robin Colenbier, Lenny Coppens, Tine Logghe, Eke van Zwol, Jean-Pierre Timmermans, Johannes Bogers

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Protocol for synthesis of spherical silver nanoparticles with stable optical properties and characterization by transmission electron microscopy by Valeria Nocerino, Bruno Miranda, Principia Dardano, Gennaro Sanità, Emanuela Esposito, Luca De Stefano

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Protocol for mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks by Rui Gao, Xiaoxue Kou, Rongwei He, Yujiang Shen, Lihong Guo, Hao Wang, Siming Huang, Guosheng Chen, Gangfeng Ouyang

“…We then detail evaluations of biocatalytic activity and recyclability.For complete details on the use and execution of this protocol, please refer to Gao et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Animal Oil, Wound Balm, Prussian Blue, the Fire and Light Principium and the Philosophers’ Stone Made from Phosphorus: on the 350th Birthday of the Chymist Johann Conrad Dippel (16... by Alexander Kraft

“…This article does not deal with his theological and philosophical views and undertakings or his medical practice, but is focused on Dippel the chymist. …”
Get full text

Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models by Apeng Chen, Yapeng Chao, Han Zou, Lauren H. McCarl, Gary Kohanbash, Baoli Hu

“…Finally, we show how to assess the tumor immune microenvironment with treatment outcomes.For complete details on the use and execution of this protocol, please refer to Chen et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Deep phenotyping characterization of human unconventional CD8+NKG2A/C+ T cells among T and NK cells by spectral flow cytometry by Aurelio Orta-Resendiz, Caroline Petitdemange, Sandrine Schmutz, Béatrice Jacquelin, Sophie Novault, Nicolas Huot, Michaela Müller-Trutwin

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

‘And she flies! Beautiful’: the dislocating geography of football sound by Margaret Trail

“…The overarching interest of this paper is in articulating the affective conditions of football’s play. It undertakes this through a consideration of the sonorous dimension of football, mapping its sounds across a framework borrowed from recent writings on sound-art and sonic geography. …”
Get full text

The problem of projects: understanding the theoretical underpinnings of project-led PBL by Roy Hanney, Maggi Savin-Baden

“…It then provides an exemplar in the form of a case study that sought to undertake such a combination, and reports on implications for practice.…”
Get full text

Developmental-stage specific single-cell human embryo dissociation by Jenna Lammers, Sophie Loubersac, Laurent David, Thomas Freour, Arnaud Reignier

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Systematics of boundary actions in gauge theory and gravity by Seolhwa Kim, Per Kraus, Richard M. Myers

“…Abstract We undertake a general study of the boundary (or edge) modes that arise in gauge and gravitational theories defined on a space with boundary, either asymptotic or at finite distance, focusing on efficient techniques for computing the corresponding boundary action. …”
Get full text

The role of natural conditions in qualified agritourism - case of Poland by Agnieszka POCZTA-WAJDA, Joanna POCZTA

“…Further, the findings suggest that, in the opinion of the hosts and guests of agritourism farms, undertaking of physical recreational activity and its intensity are influenced by the favourable sightseeing and natural conditions, the number of offered forms of physical recreation and the availability of sports equipment.…”
Get full text

Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish by Hiu-tung Candy Wong, Catherine M. Drerup

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices by Alvaro Curiel-Garcia, Amanda R. Decker-Farrell, Stephen A. Sastra, Kenneth P. Olive

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

Economic geography and entrepreneurial diversification in the agricultural sector by Sharif Rasel, Wim J. M. Heijman, Stijn Reinhard

“…Findings from the Netherlands show that the higher the concentration of entrepreneurial diversification in a region, the greater the likelihood that a farmer undertakes entrepreneurship on the farm.…”
Get full text

Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish by Bingyuan Yang, Yue Zhang, Liu Yang, Wenbiao Chen

“…We describe the preparation of zebrafish transgenic lines and detail steps for setting up the fish for live confocal imaging.For more details on the use and execution of this protocol, please refer to Yang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text