From ‘being alone’ to ‘just being': an analysis of the song ‘Preciso aprender a só ser’ by Gilberto Gil and its (con)texts by Vinícius Rangel Souto, Almir Côrtes Barreto

“…This paper undertakes an analysis of the song Preciso aprender a só ser (1973) by Gilberto Gil, evincing the links established by him between his composition and the song Preciso aprender a ser só (1965), by Marcos and Paulo Sérgio Valle. …”
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Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q by Arpit Kumar Pradhan, Qinfang Shi, Katharina Johanna Tartler, Gerhard Rammes

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for in situ visualization of mitochondrial ROS and apoptosis in spatially confined cells and sample preparation for biochemical analysis by Yijun Qi, Guoqing Cai, Weiwei Yang

“…This approach allows in situ evaluation of a variety of cellular functions during confined migration and preparation of the samples of confined cells for further biochemical analysis.For complete details on the use and execution of this protocol, please refer to Cai et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging by Tina Esmail, Maiken Nedergaard, Felix R.M. Beinlich

“…We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging.For complete details on the use and execution of this protocol, please refer to Møllgård et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Expansion microscopy of the chick embryo neural tube to overcome molecular crowding at the centrosomes-cilia by Axelle Wilmerding, Paula Espana-Bonilla, Nikolaos-Nikiforos Giakoumakis, Murielle Saade

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for in vivo and in vitro study of lymphatic valve formation driven by shear stress signaling pathway by Richa Banerjee, Luz A. Knauer, Ying Yang

“…This approach is useful to study lymphatic valve formation driven by oscillatory shear stress.For complete details on the use and execution of this protocol, please refer to Scallan et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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O catador de resíduos sólidos recicláveis e a desigualdade social by Sandra Regina Medeiros Romancini, Ednilson Viana, Teresinha Maria Gonçalves

“…We are looking forward to investigate also regarding the invisibility of the catcher of residues you recycled along with the society, even admitting the importance of your work for the urban cleaning, as well as legitimation that these workers undertake to the values of the modern society the measure that you face significant barriers for if they integrate the society for the work. …”
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Apparent coordinated and communal hunting behaviours by Erabu sea krait Laticauda semifactiata by Ruchira Somaweera, Vinay Udyawer, A. A. Thasun Amarasinghe, Joe de Fresnes, Jay Catherall, Galina Molchanova

“…Abstract Opportunistic observations of Erabu sea kraits (Laticauda semifaciata) provide evidence that this species undertake a novel foraging tactic; coordinated communal hunting. …”
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Simultaneous recordings of pupil size variation and locus coeruleus activity in mice by Marine Megemont, Lucas S. Tortorelli, Jim McBurney-Lin, Jeremiah Y. Cohen, Daniel H. O’Connor, Hongdian Yang

“…We then detail procedures for data processing and analysis.For complete details on the use and execution of this protocol, please refer to Megemont et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Characterizing diversity among human oral stem-like cancer cells using flow cytometry by Subhashis Ghosh, Paromita Mitra, Uday Saha, Kavya Vipparthi, Sandeep Singh

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Energy and CO2 emission reduction potential from investment in energy efficiency building retrofits in Bangkok, Thailand by Christopher Charles Seeley, Shobhakar Dhakal

“…Our findings suggest that by undertaking energy efficiency retrofits in Bangkok, there is the potential to reduce energy costs by approximately US123 million and reduce over 500,000 tons of CO2 emissions per year, presenting an investment opportunity of approximately US300 million, with a payback of 3.49 years.…”
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Protocol to explain graph neural network predictions using an edge-centric Shapley value-based approach by Andrea Mastropietro, Giuseppe Pasculli, Jürgen Bajorath

“…We also detail steps encompassing neural network training, an explanation phase, and analysis via feature mapping.For complete details on the use and execution of this protocol, please refer to Mastropietro et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry by Zhou Chen, Sandra Zakrzewska, Holly S. Hajare, J. Du Bois, Daniel L. Minor, Jr.

“…These assays are generalizable and can be used for other paralytic shellfish poisoning toxin-binding proteins.For complete details on the use and execution of this protocol, please refer to Chen et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for quantitative ethology on natural social interactions in Drosophila by Jing Ning, Zhou Li, Xingjiang Zhang, Junlong Wang, Dandan Chen, Qiong Liu, Yefeng Shao, Yi Sun

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Mesure imposée et engagements négociés. Mineurs, acteurs familiaux et professionnels dans le cadre de mesures d’Action éducative en milieu ouvert (AEMO) by Emilie Potin

“…What types of commitments are expected of minors and minors how do they undertake ? This article brings to light the formal spaces designed for the participation of family actors and spaces created in the educational support.…”
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Protocols for the social transfer of pain and analgesia in mice by Benjamin Rein, Erin Jones, Sabrena Tuy, Cali Boustani, Julia A. Johnson, Robert C. Malenka, Monique L. Smith

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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The EXTENSIN enigma by Steven Moussu, Gwyneth Ingram

“…However, like many other classes of cell wall proteins, EXTs are biochemically complex, highly diverse, and are encoded by multiple genes, making in-depth functional characterization a challenging undertaking. Here we will provide an overview of current knowledge of the biochemistry and properties of EXTs, and of the tools that have been deployed to study their biological functions in plants.…”
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Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues by Wenjing Liang, Rita H. Najor, Åsa B. Gustafsson

“…The isolated EVs can be used for various experiments to gain insight into their in vivo functions.For complete details on the use and execution of this protocol, please refer to Liang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome by Carmen M. Anadon, Chaomei Zhang, Xuefeng Wang, Ling Cen, Jose R. Conejo-Garcia, Xiaoqing Yu

“…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for generation and assessment of head-like structure in zebrafish by Tao Cheng, Yan-Yi Xing, Yang Dong, Peng-Fei Xu

“…We then detail assessment of this structure through axis induction.For complete details on the use and execution of this protocol, please refer to Cheng et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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