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  1. 1701

    Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation by Gavin I. Ellis, Mosha Z. Deng, Delaine W. Winn, Kimberly E. Coker, Divanshu Shukla, Vijay Bhoj, Michael C. Milone, Raimon Duran-Struuck, James L. Riley

    Published 2022-12-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  2. 1702

    Protocol for phase-field simulations of lithium dendrite growth with MOOSE framework by Shoutong Jin, Yongjun Wu, Hui Yang, Yuhui Huang, Zijian Hong

    Published 2022-12-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  3. 1703

    Protocol for recording and analyzing spontaneous locomotion in Drosophila by Taylor Barwell, Sehaj Raina, Laurent Seroude

    Published 2022-12-01
    “…We have applied this to study Drosophila movement during aging, but it could be used to address a variety of research questions.For complete details on the use and execution of this protocol, please refer to Barwell et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  4. 1704

    Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae by Kazumasa Oya, Akira Matsuura

    Published 2023-03-01
    “…This protocol can be broadly applied to evaluate the physiology of polyploid cells.For complete details on the use and execution of this protocol, please refer to Oya and Matsuura (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  5. 1705

    Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeast by Janhavi A. Kolhe, Neethu L. Babu, Brian C. Freeman

    Published 2023-12-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  6. 1706

    The COVID-19 pandemic reveals the wide-ranging role of biobanks by Dovilė Juozapaitė, Mantas Minkauskas, Inga Laurinaitytė, Martyna Simutytė, Laimonas Griškevičius, Daniel Naumovas

    Published 2023-09-01
    “…One type of organization that could undertake a major role in the process is biobank – a mediator between clinical practice and research. …”
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  7. 1707

    Protocol for live imaging of transferred mouse bone marrow cells by two-photon microscopy by Takao Sudo, Erika Yamashita, Junichi Kikuta, Masaru Ishii

    Published 2023-12-01
    “…We then detail procedures for creating images and analyzing cells.For complete details on the use and execution of this protocol, please refer to Sudo et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  8. 1708

    Protocol for the rapid intravenous in ovo injection of developing amniote embryos by Rory L. Cooper, Gabriel Santos-Durán, Michel C. Milinkovitch

    Published 2023-06-01
    “…This technique is rapid, is reproducible, is of low cost, and will provide an important resource for developmental biologists.For complete details on the use and execution of this protocol, please refer to Cooper & Milinkovitch.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  9. 1709

    Protocol for deep proteomic profiling of formalin-fixed paraffin-embedded specimens using a spectral library-free approach by Rajesh Kumar Soni

    Published 2023-09-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  10. 1710

    Protocols for genetic labeling and tracing of Staphylococcus xylosus during tumor progression by Tingting Dong, Aikun Fu, Shang Cai

    Published 2022-12-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  11. 1711

    Integrative detection of genome-wide translation using iRibo by Alistair Turcan, Jiwon Lee, Aaron Wacholder, Anne-Ruxandra Carvunis

    Published 2024-03-01
    “…We describe the process of using iRibo to generate a species’ translatome from a set of ribosome profiling samples using S. cerevisiae as an example.For complete details on the use and execution of this protocol, please refer to Wacholder et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  12. 1712

    Significance Evaluation of Environmental Aspects by Marcela Malindzakova

    Published 2011-09-01
    “…In order to effectively reduce negative environmental impacts, first it is necessary to undertake a thorough examination of given environmental aspects. …”
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  13. 1713

    A protocol to establish low-cost floating treatment wetlands for large-scale wastewater reclamation by Muhammad Arslan, Samina Iqbal, Ejazul Islam, Mohamed Gamal El-Din, Muhammad Afzal

    Published 2023-12-01
    “…We describe steps for setting up a plant nursery, designing and establishing the FTWs, vegetating units, and building the FTW island.For complete details on the use and execution of this protocol, please refer to Afzal et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  14. 1714

    Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli by Jil-Christine Kramer, Nils Böhringer, Ute Mettal, Till F. Schäberle

    Published 2023-09-01
    “…We then detail the expression of a His-tagged gene to deduce the specific function of the protein.For complete details on the use and execution of this protocol, please refer to Böhringer et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  15. 1715

    A protocol to detect human CD4+ T cell extracellular traps using scanning electron microscopy by Nam Phuong N. Nguyen, Nicole Chizara Oparaugo, Kelsey Ouyang, George W. Agak

    Published 2023-03-01
    “…This protocol can be applied to the study of other ETs released by other T cell subsets.For complete details on the use and execution of this protocol, please refer to Agak et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  16. 1716

    Reconstructing cancer phylogenies using Pairtree, a clone tree reconstruction algorithm by Ethan Kulman, Jeff Wintersinger, Quaid Morris

    Published 2022-12-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  17. 1717

    Student Absenteeism in Mathematics Lessons: Social Variables in the PGS of Namibe by Eduardo Chaves-Barboza, José-Antonio Marín-Marín, Ana-Paula Sarmento-dos-Santos, Juan-Manuel Trujillo-Torrres

    Published 2019-06-01
    “…It contrasts teachers’ perceptions with the perceptions of students undertaking their second and third levels of a Bachelor’s degree in Physics and Chemistry at the Pedagogical Graduate School (PGS) of Namibe, Angola. …”
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  18. 1718

    On the Similarity and Dependence of Time Series by Vilém Novák, Soheyla Mirshahi

    Published 2021-03-01
    “…In this paper, we undertake the problem of evaluating interrelation among time series. …”
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  19. 1719

    Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices by Tori Lhomme, Vincent Prévot

    Published 2023-12-01
    “…While we use, as an example, tanycytes of the arcuate nucleus of the hypothalamus and pro-opiomelanocortin neurons, this protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population.For complete details on the use and execution of this protocol, please refer to Lhomme et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  20. 1720

    Isolation and detection of extracellular vesicles from melanoma cells and liquid biopsies using size-exclusion chromatography and nano-flow cytometry by Evelyn Lattmann, Valérie Lapaire, Mitchell P. Levesque

    Published 2023-09-01
    “…. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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