Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (<i>Acipenser ruthenus</i>) by Ievgeniia Gazo, Ravindra Naraine, Ievgen Lebeda, Aleš Tomčala, Mariola Dietrich, Roman Franěk, Martin Pšenička, Radek Šindelka

“…We observed the upregulated expression of zona pellucida sperm-binding proteins in all treatment groups, as well as the upregulation of several glycolytic enzymes. …”
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The mechanisms of MicroRNA 21 in premature ovarian insufficiency mice with mesenchymal stem cells transplantation by Na Yin, Chao Luo, Lun Wei, Guangzhao Yang, Le Bo, Caiping Mao

“…Mice hormone secretion and the anti-Zona pellucida antibody (AZPAb) levels were analyzed, the ovarian morphological changes and folliculogenesis were observed, and the ovarian apoptosis cells were detected to evaluate ovarian function. …”
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A Quantitative Histologic Analysis of Oogenesis in the Flatfish Species <i>Pleuronectes platessa</i> as a Tool for Fisheries Management by Carine Sauger, Jérôme Quinquis, Clothilde Berthelin, Mélanie Lepoittevin, Nicolas Elie, Laurent Dubroca, Kristell Kellner

“…The following paper gives a detailed description of the oogenesis cycle for the European Plaice (<i>Pleuronectes platessa</i>), from oogonia to post-ovulatory follicle, including ovarian follicle and <i>zona pellucida</i> sizes. Noteworthy particularities were the difficulty in identifying cortical alveoli due to their very small size. …”
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Granulosa Cell Specific Loss of Adar in Mice Delays Ovulation, Oocyte Maturation and Leads to Infertility by Rikki N. Nelson, V. Praveen Chakravarthi, Anamika Ratri, Xiaoman Hong, Jan A. Gossen, Lane K. Christenson

“…Temporal expression analysis of GCs at 0, 4, 8, and 12 h post-hCG of <i>Ifi44</i>, <i>Ifit1</i>, <i>Ifit3b</i>, and <i>Oas1g</i> and <i>Ovgp1</i> confirmed upregulation of these inflammatory and interferon genes and downregulation of <i>Ovgp1</i> a glycoprotein involved in oocyte zona pellucida stability. Thus, loss of ADAR1 in GCs leads to increased expression of inflammatory and interferon response genes which are temporally linked to ovulation failure, alterations in oocyte developmental progression and infertility.…”
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Detecting Blastocyst Components by Artificial Intelligence for Human Embryological Analysis to Improve Success Rate of In Vitro Fertilization by Muhammad Arsalan, Adnan Haider, Jiho Choi, Kang Ryoung Park

“…The embryologist evaluates blastocyst viability by manual microscopic assessment of its components, such as zona pellucida (ZP), trophectoderm (TE), blastocoel (BL), and inner cell mass (ICM). …”
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Investigating the Ovarian Microstructure in the Genera <i>Helicolenus</i> and <i>Scorpaena</i> (Teleostei, Sub-Order Scorpaenoidei) with Implications for Ovarian Dynamics and Spawn... by Cristina Porcu, Eleonora Lai, Andrea Bellodi, Pierluigi Carbonara, Alessandro Cau, Antonello Mulas, Noemi Pascale, Riccardo Porceddu, Maria Cristina Follesa

“…Some microscopic peculiarities in the oogenesis process were found: thin zona pellucida, small and low cortical alveoli, and a specialized ovarian wall during the spawning period. …”
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Assessment of transcript and protein levels contributing to cell cycle control and gap junction connections in morphologically variable groups of porcine cumulus-oocyte complexes by P. Antosik, B. Kempisty, M. Jackowska, H. Piotrowska, D. Bukowska, M. Wozna, M. Lianeri, K.-P. Brussow, J.M. Jaskowski

“…The cdk4 protein in oocytes graded I and II is localized mainly in the zona pellucida, although in grade III COCs the expression of this protein is decreased and observed only in the cytoplasm. …”
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Oviductal estrogen receptor α signaling prevents protease-mediated embryo death by Wipawee Winuthayanon, Miranda L Bernhardt, Elizabeth Padilla-Banks, Page H Myers, Matthew L Edin, Fred B Lih, Sylvia C Hewitt, Kenneth S Korach, Carmen J Williams

“…Elevated protease activity in cKO oviducts causes premature degradation of the zona pellucida and embryo lysis, and wild-type embryos transferred into cKO oviducts fail to develop normally unless rescued by concomitant transfer of protease inhibitors. …”
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Homozygous Splice Site Mutation in <i>ZP1</i> Causes Familial Oocyte Maturation Defect by Özlem Okutman, Cem Demirel, Firat Tülek, Veronique Pfister, Umut Büyük, Jean Muller, Nicolas Charlet-Berguerand, Stéphane Viville

“…Analysis of the data reveals a homozygous splice site mutation (c.1775-3C>A) in the zona pellucida glycoprotein 1 (<i>ZP1</i>) gene. Minigene experiments show that the mutation causes the retention of the intron 11 sequence between exon 11 and exon 12, resulting in a frameshift and the likely production of a truncated protein.…”
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Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus) by Md. Rashedul ISLAM, Osamu ICHII, Teppei NAKAMURA, Takao IRIE, Md. Abdul MASUM, Marina HOSOTANI, Yuki OTANI, Yaser Hosny Ali ELEWA, Yasuhiro KON

“…Almost all MOF oocytes and a few DNOs possessed a clear zona pellucida, expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and Forkhead box protein 2, a representative marker of oocytes and follicular epithelial cells. …”
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Oocyte Morphometric Assessment and Gene Expression Profiling of Oocytes and Cumulus Cells as Biomarkers of Oocyte Competence in Sheep by Carolina Maside, Irene Sánchez-Ajofrín, Daniela Medina-Chávez, Benner Alves, José Julián Garde, Ana Josefa Soler

“…In experiment 1, individually matured oocytes were measured and classified into small, intermediate, and large oocytes after a cluster analysis, based on total diameter (with zona pellucida, ZP), oocyte diameter (without ZP), and ZP thickness. …”
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Evaluation of Stress Accompanying Immunocontraceptive Vaccination in Donkeys by Erik W. Peterson, Lorenzo G. T. M. Segabinazzi, Robert O. Gilbert, Don R. Bergfelt, Hilari M. French

“…Therefore, this study sought to evaluate the stress imposed by the use of two formulations of a zona pellucida (ZP) vaccine, a recombinant (reZP) and a native porcine (pZP) vaccine, both formulated with a Freund’s adjuvant. …”
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Characterization of basal seminal traits and semen cryopreservation in Canada lynx (Lynx canadensis) by Raquel González, Amy Miller, Lindsey Marie Vansandt, William F. Swanson

“…Cleavage rates did not differ between TEY (29 ± 17 %) and SOY (27 ± 14 %) and mean numbers of sperm bound to the zona pellucida was similar (20 sperm, TEY vs. 18 sperm, SOY), indicating that both extenders equally preserved the function of Canada lynx sperm for semen banking purposes. …”
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Dusky-like Is Critical for Morphogenesis of the Cellular Protuberances and Formation of the Cuticle in <i>Henosepilachna vigintioctopunctata</i> by Yuxing Zhang, Qiao Tan, Mengjiao Lin, Chenhui Shen, Lin Jin, Guoqing Li

“…Dusky-like (Dyl) is a transmembrane protein containing a zona pellucida domain. Its physiological roles during metamorphosis have been well explored in <i>Drosophila melanogaster</i> and have also been documented in <i>Tribolium castaneum</i>. …”
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Effect of a Combination of Myo-Inositol, Alpha-Lipoic Acid, and Folic Acid on Oocyte Morphology and Embryo Morphokinetics in non-PCOS Overweight/Obese Patients Undergoing IVF: A Pi... by Stefano Canosa, Carlotta Paschero, Andrea Carosso, Sara Leoncini, Noemi Mercaldo, Gianluca Gennarelli, Chiara Benedetto, Alberto Revelli

“…Oocytes were examined using Polarized Light Microscopy to assess morphological features of zona pellucida (ZP) and meiotic spindle (MS). One hundred and seventy-six embryos (<i>n</i> = 84 in the MI + ALA + FA group, <i>n</i> = 92 in the FA-only group) were assessed by conventional morphology on days 2 and 5, as well as using the Time-Lapse System morphokinetic analysis. …”
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Specific expression of alternatively spliced genes in the turkey (Meleagris gallopavo) reproductive tract revealed their function in spermatogenesis and post-testicular sperm matur... by Łukasz Paukszto, Joanna Wiśniewska, Ewa Liszewska, Marta Majewska, Jan Jastrzębski, Jan Jankowski, Andrzej Ciereszko, Mariola Słowińska

“…Predicted differentially ASGs in the turkey reproductive tract seem to be involved in the regulation of spermatogenesis, including acrosome formation and sperm tail formation and binding of sperm to the zona pellucida. Several ASGs were classified as cilia by actin and microtubule cytoskeleton organization. …”
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Cryotolerance of in vitro matured and vitrified bovine oocytes supplemented with L-carnitine and niacin by K. Anjitha, A. Amritha, R. S. Abhilash, C. Jayakumar, T. Marykutty

“…Shrinkage of cytoplasm was the most common type of cryodamage observed in all the three groups followed by cracked zona pellucida, abnormal shape and increased peri vitelline space. …”
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The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes by Michaela Frolikova, Tereza Otcenaskova, Eliska Valasková, Pavla Postlerova, Romana Stopkova, Pavel Stopka, Katerina Komrskova

“…This AR onset is independent of the extracellular matrix of the oocyte called <i>zona pellucida</i>. After AR, the relocation of mTAS1R3 to the equatorial segment was observed and the receptor remained exposed to the outer surroundings of the female reproductive tract, where its physiological ligand, the amino acid <i>L-glutamate</i>, naturally occurs. …”
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Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity by Mona M. Elghareeb, Gehad E. Elshopakey, Shaymaa Rezk, Ahmed Ateya, Eman S. El-Ashry, Mustafa Shukry, Heba I. Ghamry, Badriyah S. Alotaibi, Nada M.A. Hashem

“…NSO also up-regulated antioxidant enzymes, anti-apoptotic protein, zona pellucida sperm-binding protein, bone morphogenetic protein, and thyroid stimulating hormone, conversely down-regulated inflammatory cytokines, apoptotic proteins, estrogen receptor-α, estrogen receptor-β, and thyroid stimulating hormone receptors compared to NaF-intoxicated group. …”
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Comparative proteomic analysis of seminal plasma exosomes in buffalo with high and low sperm motility by Kai Yu, Kai Xiao, Qin-qiang Sun, Run-feng Liu, Liang-feng Huang, Peng-fei Zhang, Hui-yan Xu, Yang-qing Lu, Qiang Fu

“…Gene Ontology (GO) enrichment of differentially expressed proteins (DEPs) showed that most differential proteins were located in sperm and vesicles, with activities of hydrolase and metalloproteinase, and were involved in sperm-egg recognition, fertilization, single fertilization, and sperm-zona pellucida binding processes, etc. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differential proteins were mainly involved in the PPRP signaling pathway, calcium signaling pathway, and cAMP signaling pathway, among others. …”
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