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201
Optimization of total RNA extraction from sperm and detection of phospholipase C zeta gene fragments from sperm, testis and epididymis of Oryctolagus cuniculus
Published 2013“…As capacitated sperm fuse with the ovum, the acrosome reaction occurs to digest the corona radiata and zona pellucida that surrounds the ovum. A single sperm will then bind to the oocyte's membrane receptor, while its nucleus is engulfed by the ooplasm. …”
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Thesis -
202
The pair ceramide 1-phosphate/ceramide kinase regulates intracellular calcium and progesterone-induced human sperm acrosomal exocytosis
Published 2023-03-01“…Before fertilization, spermatozoa must undergo calcium-regulated acrosome exocytosis in response to physiological stimuli such as progesterone and zona pellucida. Our laboratory has elucidated the signaling cascades accomplished by different sphingolipids during human sperm acrosomal exocytosis. …”
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203
Early-Life Exposure to the Mycotoxin Fumonisin B1 and Developmental Programming of the Ovary of the Offspring: The Possible Role of Autophagy in Fertility Recovery
Published 2023-12-01“…Histopathological changes were evidenced by the altered structures of most of the growing follicle oocytes, as revealed by a thinning irregular zona pellucida and pyknosis in granulosa cells. These findings are concomitant with steroidogenesis- and folliculogenesis-related gene expression, as evidenced by the decrease in CYP19 activity and estrogen receptor beta (ESR2) gene expression. …”
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204
Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma in Mice and Rats
Published 2022-01-01“…Then, the acrosome-reacted spermatozoa penetrate the zona pellucida and adhere to and finally fuse with the egg plasma membrane. …”
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205
Identification and Functional Assignment of Genes Implicated in Sperm Maturation of Tibetan Sheep
Published 2023-05-01“…Functional annotation revealed that they were mainly enriched to various distinct biological processes associated with reproduction (including the caput binding of sperm to the zona pellucida; fertilization in the caput and corpus; and meiosis in the caput and cauda) and development (such as cell differentiation and developmental maturation in the caput; cell proliferation and metabolism in the corpus; and regulation of tube size and cell division/cell cycle in the cauda). …”
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206
Pyrroloquinoline Quinone Improves Ram Sperm Quality through Its Antioxidative Ability during Storage at 4 °C
Published 2024-01-01“…Furthermore, the value of the sperm–zona pellucida binding capacity in the 100 nM PQQ treatment group was also much higher than that of the control. …”
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207
Suppression of Non-Random Fertilization by MHC Class I Antigens
Published 2020-11-01“…Moreover, <i>T-KO</i> sperm showed multiple fusion with zona pellucida (ZP)-free <i>WT</i> eggs, implying that the ability of polyspermy block for sperm from <i>T-KO</i> males was weakened in <i>WT</i> eggs. …”
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208
Laser-Assisted In Vitro Fertilization Facilitates Fertilization of Vitrified-Warmed C57BL/6 Mouse Oocytes with Fresh and Frozen-Thawed Spermatozoa, Producing Live Pups
Published 2014“…Due to hardening of the zona pellucida, spermatozoa are often unable to penetrate vitrified-warmed (V-W) oocytes. …”
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209
Vision-guided robotic micromanipulation for cell rotation control
Published 2024“…Meanwhile, an image processing system built on the basis of U-Net is developed for selecting the optimal dissection position in the zona pellucida (ZP). The system is proven reliable as it effectively indicated the optimal dissection position in the ZP with a success rate of 95.6%. …”
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Thesis-Doctor of Philosophy -
210
Adenovirus-vectored immunocontraception in rat model
Published 2012“…The critical involvement of the female gamete protein zona pellucida 3 (ZP3) in the fertilization process has been well documented. …”
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Thesis -
211
Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmenta...
Published 2020-07-01“…The egg cell is prepared to prevent polyspermia by, among other ways, producing cortical granules (CGs), which are specialized intracellular structures containing enzymes that aim to harden the zona pellucida and block the fusion of subsequent sperm. …”
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212
Mutations in FIGLA Associated With Premature Ovarian Insufficiency in a Chinese Population
Published 2021-10-01“…Luciferase reporter genes were used to determine the effect of wild-type and mutant FIGLA genotypes on the transcriptional activity of its downstream targets, the zona pellucida glycoprotein genes ZP1, ZP2, and ZP3. Chromatin immunoprecipitation was used to determine the level of binding between wild-type and mutant FIGLA with the ZP1, ZP2, and ZP3 promoters.Results: Three different FIGLA mutations were identified in four patients with POI. …”
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213
The impact of asymptomatic and mild COVID-19 on sperm characteristics
Published 2022-10-01“…Mild and asymptomatic forms of the new coronavirus infection course can negatively affect the interaction of spermatozoa with hyaluronic acid (HBA test), that indicates the impairment of the spermatozoa binding to the zona pellucida. One should pay attention to the HBA test when examining the sperm of patients who have suffered the new coronavirus infection.…”
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214
Development Of An Immunocontraceptive Vaccine For Biocontrol Of Rats
Published 2004“…Zona pellucida (ZP) is an extracellular matrix that surrounds the mammalian oocyte. …”
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Thesis -
215
The vertebrate- and testis- specific transmembrane protein C11ORF94 plays a critical role in sperm-oocyte membrane binding
Published 2022-09-01“…Sperm from C11orf94-deficient mice could pass through the zona pellucida, but failed to bind to the oocyte membrane, thus accumulating in the perivitelline space. …”
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216
Oocyte-Specific Knockout of Histone Lysine Demethylase KDM2a Compromises Fertility by Blocking the Development of Follicles and Oocytes
Published 2022-10-01“…To elucidate the physiological role of <i>Kdm2a</i>, the zona pellucida 3-Cre (<i>Zp3</i>-<i>Cre</i>)/LoxP system was used to generate an oocyte <i>Kdm2a</i> conditional knockout (<i>Zp3</i>-<i>Cre</i>; <i>Kdm2a<sup>flox/flox</sup></i>, termed <i>Kdm2a</i> cKO) model. …”
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217
Effects of butylated hydroxytoluene on protein P25b, chilled and cryopreserved bull semen in Bioxcell®, tris- and citrate-egg yolk extenders
Published 2016“…The sperm surface P25b is considered a bull fertility marker, very important in zona pellucida binding during fertilization. Thus, the fertility of spermatozoa may be preserved if P25b is protected. …”
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Thesis -
218
Oocyte differentiation is genetically dissociable from meiosis in mice
Published 2014“…Here we report Stra8-deficient ovarian germ cells that grow and differentiate into oocyte-like cells that synthesize zonae pellucidae, organize surrounding somatic cells into follicles, are ovulated in response to hormonal stimulation, undergo asymmetric cell division to produce a polar body and cleave to form two-cell embryos upon fertilization. …”
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219
Production of calves following nonsurgical transfer of fresh and refrigerated bovine demi-embryos
Published 2008-12-01“…The viability of microblade-bisected Day-7 embryos transferred to recipients without zona pellucidae directly after splitting or after overnight storage at 4°C was investigated in two experiments. …”
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220
Effects of N-acetylcysteine on Growth, Viability, and Ultrastructure of In Vitro Cultured Bovine Secondary Follicles
Published 2022-11-01“…Ultrastructural analysis showed that oocytes from follicles cultured in control medium alone or with 1 mM NAC had intact zonae pellucidae in close association with oolemmae, but the ooplasm showed mitochondria with a reduced number of cristae. …”
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