Sperm Adhesion Molecule 1 (SPAM1) Distribution in Selected Human Sperm by Hyaluronic Acid Test by María José Gómez-Torres, Paula Sáez-Espinosa, Paula Manzano-Santiago, Laura Robles-Gómez, Natalia Huerta-Retamal, Jon Aizpurua

“…The failures of binding to the oocyte zona pellucida are commonly attributed to defects in the sperm recognition, adhesion, and fusion molecules. …”
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Genomic disturbance of vitellogenin 2 (vtg2) leads to vitellin membrane deficiencies and significant mortalities at early stages of embryonic development in zebrafish (Danio rerio) by Ozlem Yilmaz, Emmanuelle Com, Charles Pineau, Julien Bobe

“…Overrepresentation of histones, zona pellucida proteins, lectins, and protein degradation related proteins in F3 vtg2-mutant embryos provide evidence to impaired mechanisms involved in vitellin membrane formation. …”
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Laissez-Faire Stallions? Males’ Fecal Cortisol Metabolite Concentrations Do Not Vary with Increased Female Turnover in Feral Horses (<i>Equus caballus</i>) by Maggie M. Jones, Cassandra M. V. Nuñez

“…In a population of feral horses (<i>Equus caballus</i>) on Shackleford Banks, North Carolina, females previously immunized with the immunocontraceptive agent porcine zona pellucida (PZP) change social groups (bands) more often than unimmunized females, disrupting the social stability within the population. …”
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Oocyte activation and fertilisation: crucial contributors from the sperm and oocyte by Yeste, M, Jones, C, Amdani, S, Coward, K

“…First, we describe the initial interaction between sperm and the zona pellucida, with particular regard to acrosome exocytosis. …”

A method for semi-automatic grading of human blastocyst microscope images. by Santos Filho, E, Noble, J, Poli, M, Griffiths, T, Emerson, G, Wells, D

“…METHODS: The delineation of the boundaries (segmentation) of the zona pellucida, trophectoderm (TE) and inner cell mass (ICM) were performed using advanced image analysis techniques (level set, phase congruency and fitting of ellipse methods). …”

Uromodulin mutations causing familial juvenile hyperuricaemic nephropathy lead to protein maturation defects and retention in the endoplasmic reticulum. by Williams, SE, Reed, A, Galvanovskis, J, Antignac, C, Goodship, T, Karet, F, Kotanko, P, Lhotta, K, Morinière, V, Williams, P, Wong, W, Rorsman, P, Thakker, R

“…Uromodulin contains three epidermal growth factor (EGF)-like domains, a cysteine-rich region which includes a domain of eight cysteines and a zona pellucida (ZP) domain. Over 90% of UMOD mutations are missense, and 62% alter a cysteine residue, implicating a role for protein misfolding in the disease. …”

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility by Arumugam Kumaresan, Manish Kumar Sinha, Nilendu Paul, Pradeep Nag, John Peter Ebenezer Samuel King, Rakesh Kumar, Tirtha Kumar Datta

“…Using high-throughput mass spectrometry platform, we identified 1305 proteins from buffalo spermatozoa and found that these proteins were mostly enriched in glycolytic process, mitochondrial respiratory chain, tricarboxylic acid cycle, protein folding, spermatogenesis, sperm motility and sperm binding to zona pellucida (p < 7.74E−08) besides metabolic (p = 4.42E−31) and reactive oxygen species (p = 1.81E−30) pathways. …”
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Comparative Proteomics of the <i>Acanthopagrus schlegelii</i> Gonad in Different Sex Reversal by Shuyin Chen, Yunxia Yang, Bo Gao, Chaofeng Jia, Fei Zhu, Qian Meng, Zhiwei Zhang, Zhiyong Zhang, Shixia Xu

“…In total, 4692 proteins were identified, including common sex-specific proteins, such as sperm-associated antigen 6 and cilia- and flagella-associated proteins in males, and zona pellucida sperm-binding proteins in females. Furthermore, proteins involved in the integrin signaling pathway, inflammation mediated by the chemokine and cytokine signaling pathways, pyruvate metabolism, CCKR signaling map, de novo purine biosynthesis and the ubiquitin proteasome pathway were upregulated in female gonads, whereas proteins implicated in DNA replication, the heterotrimeric G-protein signaling pathway, Gi alpha- and Gs alpha-mediated pathways, wnt signaling pathway, and hedgehog signaling pathway were upregulated in male gonads. …”
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Identification, characterization and metagenome analysis of oocyte-specific genes organized in clusters in the mouse genome by Vaiman Daniel, Dalbiès-Tran Rozenn, Bontoux Martine, Callebaut Isabelle, Dadé Sébastien, Paillisson Amélie, Monget Philippe

“…</p> <p>Results</p> <p>In the present work, five clusters have been studied: a cluster of thirteen genes characterized by an F-box domain localized on chromosome 9, a cluster of six genes related to T-cell leukaemia/lymphoma protein 1 (Tcl1) on chromosome 12, a cluster composed of a SPErm-associated glutamate (E)-Rich (Speer) protein expressed in the oocyte in the vicinity of four unknown genes specifically expressed in the testis on chromosome 14, a cluster composed of the oocyte secreted protein-1 (Oosp-1) gene and two Oosp-related genes on chromosome 19, all three being characterized by a partial N-terminal zona pellucida-like domain, and another small cluster of two genes on chromosome 19 as well, composed of a TWIK-Related spinal cord K+ channel encoding-gene, and an unknown gene predicted <it>in silico </it>to be testis-specific. …”
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The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening by Timur Shkrigunov, Victor Zgoda, Peter Klimenko, Anna Kozlova, Maria Klimenko, Andrey Lisitsa, Mark Kurtser, Natalia Petushkova

“…Among the abundant terms for GO cellular components, zona pellucida receptor complex, sperm fibrous sheath, and outer dense fiber were revealed. …”
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Hatchability evaluation of bovine IVF embryos using OCT-based 3D image analysis by Yasumitsu MASUDA, Ryo HASEBE, Yasushi KUROMI, Mitsugu HISHINUMA, Tetsuya OHBAYASHI, Ryo NISHIMURA

“…The thickness of the trophectoderm (TE) and TE+zona pellucida (ZP) was lesser, and the volumes of the TE, ZP, blastocoel, and whole embryo and blastocoel diameter were greater in the H embryos than in the NH embryos. …”
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Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos by Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara, Takeshige Otoi

“…A mosaic mutation was observed in blastocysts derived from zona pellucida (ZP)-free oocytes following lipofection treatment, regardless of the target genes. …”
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Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM by Chongyi Shu, Yiqi Yu, Xiaopan Chen, Jiansheng Guo, Yier Zhou, Dandan Wu, Tianyun Yang, Yuhang Fan, Qiongxiao Huang, Jing Shu

“…Abstract The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. …”
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Glyphosate Interference in Follicular Organization in the Wall Lizard <i>Podarcis siculus</i> by Luigi Rosati, Teresa Chianese, Vincenza De Gregorio, Mariailaria Verderame, Anja Raggio, Chiara Maria Motta, Rosaria Scudiero

“…It also induced thecal fibrosis and affected oocyte cytoplasm and zona pellucida organizations. At the functional levels, Gly stimulated the synthesis of estrogen receptors, suggesting a serious endocrine-disrupting effect. …”
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Effect of Antioxidant Supplementation on the Sperm Proteome of Idiopathic Infertile Men by Ashok Agarwal, Manesh Kumar Panner Selvam, Luna Samanta, Sarah C. Vij, Neel Parekh, Edmund Sabanegh, Nicholas N. Tadros, Mohamed Arafa, Rakesh Sharma

“…Further, activation of TRiC complex helped in nuclear compaction, maintenance of telomere length, flagella function, and expression of zona pellucida receptors for sperm&#8722;oocyte interaction.…”
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Retinoic acid enhances germ cell differentiation of mouse skin-derived stem cells by Paul W. Dyce, Neil Tenn, Gerald M. Kidder

“…RA treatment also improved the structural integrity of the OLCs produced by initiating the expression of all three zona pellucida transcripts (Zp1–3) and improving ZP3 expression levels and localization. …”
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A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in Caenorhabditis elegans by Jennifer D Cohen, Alessandro P Sparacio, Alexandra C Belfi, Rachel Forman-Rubinsky, David H Hall, Hannah Maul-Newby, Alison R Frand, Meera V Sundaram

“…Here we describe the vulva luminal matrix, which contains chondroitin proteoglycans, Zona Pellucida (ZP) domain proteins, and other glycoproteins and lipid transporters related to those in mammals. …”
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Assisting the Human Embryo Viability Assessment by Deep Learning for In Vitro Fertilization by Muhammad Ishaq, Salman Raza, Hunza Rehar, Shan e Zain ul Abadeen, Dildar Hussain, Rizwan Ali Naqvi, Seung-Won Lee

“…The success of IVF mainly depends on the assessment and analysis of human blastocyst components such as the blastocoel (BC), zona pellucida (ZP), inner cell mass (ICM), and trophectoderm (TE). …”
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Critical Role of the Cortical Alveolus Protease Alveolin in Chorion Hardening In Vivo at Medaka Fertilization by Bo Fu, Di Wu, Shigeki Yasumasu, Masaya Hane, Chihiro Sato, Ken Kitajima

“…Purified alveolin is known to induce chorion hardening in vitro by processing zona pellucida B (ZPB), a major chorion component. However, in vivo function of alveolin remains unclear; thus, in this study, the effects of <i>alveolin</i> efficiency (<i>Alv</i>^−/−) at the organism level were investigated using the medaka, <i>Oryzias latipes</i>. …”
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#328 : Fertilization and Embryo Quality of Human Mature Oocytes with Morphological Abnormalities After Intracytoplasmic Sperm Injection (ICSI) by Jaqueline Sudiman, IB Putra Adnyana, I Gusti Ayu Widianti, Anom Suardika

“…Mature oocytes were classified as normal morphology (control), oocytes with intra-cytoplasmic dysmorphism (fragile oocytes, inclusion bodies, vacuoles, mild granularity of cytoplasm, severe granularity of cytoplasm, and central granulation), and oocytes with extra-cytoplasmic dysmorphism (granulation in perivitelline space, polar body fragmentation, large perivitelline space, and abnormal zona pellucida). All oocytes were inseminated with ICSI. …”
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