Development of a Lung Vacancy Mouse Model through CRISPR/Cas9-Mediated Deletion of Thyroid Transcription Factor 1 Exon 2 by Lihua Zhao, Meishuang Li, Zhibao Yin, Limin Lv, Meng Zhou, Yixi Wang, Manling Zhang, Tianxu Guo, Xiyun Guo, Han Liu, Linxin Cheng, Xiubin Liang, Shuguang Duo, Rongfeng Li

“…To improve the mouse model towards achieving SCOG-based lung generation, in this project, we used the CRISPR/Cas9 tool to remove Exon 2 of the gene by zygote microinjection and successfully produced <i>TTF-1</i> knockout (E2del) mice. …”
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The impact of oocytes containing smooth endoplasmic reticulum aggregates on assisted reproductive outcomes: a cohort study by Tingfeng Fang, Wenchang Yu, Songbang Ou, Jinyu Lu, Ruiqi Li, Mingpeng Zhao, Yiu Leung Chan, Wenjun Wang

“…Comparable two pronuclei (2PN) fertilization rate and poly-pronucleus zygote rate were shown in SERa + and SERa- cycles (P > 0.05), but which were higher in SERa + oocytes than in SERa- oocytes (P < 0.05). …”
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Adiposity and lipid metabolism indicators mediate the adverse effect of glucose metabolism indicators on oogenesis and embryogenesis in PCOS women undergoing IVF/ICSI cycles by Huahua Jiang, Manfei Si, Tian Tian, Huifeng Shi, Ning Huang, Hongbin Chi, Rui Yang, Xiaoyu Long, Jie Qiao

“…The mediation analysis indicated that elevated FPG, 2hPG, FPI, 2hPI, HbA1c, and HOMA2-IR were significantly associated with decreased retrieved oocyte count, MII oocyte count, normally fertilized zygote count, normally cleaved embryo count, high-quality embryo count, or blastocyst formation count after controlling for adiposity and lipid metabolism indicators. …”
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Isoform-resolved transcriptome of the human preimplantation embryo by Denis Torre, Nancy J. Francoeur, Yael Kalma, Ilana Gross Carmel, Betsaida S. Melo, Gintaras Deikus, Kimaada Allette, Ron Flohr, Maya Fridrikh, Konstantinos Vlachos, Kent Madrid, Hardik Shah, Ying-Chih Wang, Shwetha H. Sridhar, Melissa L. Smith, Efrat Eliyahu, Foad Azem, Hadar Amir, Yoav Mayshar, Ivan Marazzi, Ernesto Guccione, Eric Schadt, Dalit Ben-Yosef, Robert Sebra

“…Here, we generate an isoform-resolved transcriptome of early human development by performing long- and short-read RNA sequencing on 73 embryos spanning the zygote to blastocyst stages. We identify 110,212 unannotated isoforms transcribed from known genes, including highly conserved protein-coding loci and key developmental regulators. …”
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Reproduction, embryonic and early post-embryonic development of saffron cod <i>Eleginus gracilis</i> (Gadidae) from the Tauyskaya Guba Bay (northern Okhotsk Sea) by Ravil R. Yusupov, Marina V. Rakitina

“…The second and third cycles of the zygote division with forming of 4 and 8 blastomers pass in the age interval 14-18 h. …”
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The Caspase Homologues in Scallop <i>Chlamys farreri</i> and Their Expression Responses to Toxic Dinoflagellates Exposure by Zhongcheng Wei, Wei Ding, Moli Li, Jiaoxia Shi, Huizhen Wang, Yangrui Wang, Yubo Li, Yiqiang Xu, Jingjie Hu, Zhenmin Bao, Xiaoli Hu

“…Almost all of the caspase-2/9 genes were highly expressed throughout all developmental stages from zygote to juvenile, and their expression in the digestive gland and kidney was slightly influenced by PSTs. …”
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Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> by Rui Fan, Yundong Li, Qibin Yang, Song Jiang, Jianhua Huang, Lishi Yang, Xu Chen, Falin Zhou, Shigui Jiang

“…<i>PmGrx2</i> was continuously expressed during development and had the highest expression level in the zygote stage. Both ammonia-N stress and bacterial infection could differentially induce the expression of <i>PmGrx2</i> in hepatopancreas and gills. …”
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Associations of personal care products use with reproductive outcomes of IVF/ICSI treatment by Qing-Chun Guo, Wen Yao, Chong Liu, Tao-Ran Deng, Juan Li, Hong-Mei Liao, Wen-Qu Tian, Yi Wang, Yao-Yao Du, Yu-Feng Li

“…Retrieved oocyte number, mature oocyte number, two distinct pronuclei (2PN) zygote number, fertilization rate, cleavage rate, blastocyst formation rate, implantation, clinical pregnancy, miscarriage, and live birth were followed up as reproductive endpoints. …”
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Early development of Rutilus frisii kutum kamenskii larvae with emphasis on the ontogeny of digestive tract by Jafari, Mahdieh

“…After hatching, daily observations were made until the fingerling stage. Nine main stages (zygote, cleavage, morula, blastula,gastrula, neurula, segmentation, pharyngula and hatching) and 30 sub-stages were observed during the embryonic development of kutum while three main stages and eighteen sub-stages were noted after the hatching until the juvenile stage. …”
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H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin by Alberto de la Iglesia, Paula Jauregi, Meritxell Jodar, Ferran Barrachina, Lukas Ded, Carme Mallofré, Leonardo Rodríguez-Carunchio, Juan Manuel Corral, Josep Lluís Ballescà, Katerina Komrskova, Judit Castillo, Rafael Oliva

“…Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote.…”
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Plasmodium berghei leucine-rich repeat protein 1 downregulates protein phosphatase 1 activity and is required for efficient oocyst development by Aline Fréville, Bénédicte Gnangnon, Annie Z. Tremp, Caroline De Witte, Katia Cailliau, Alain Martoriati, El Moukthar Aliouat, Priyanka Fernandes, Cerina Chhuon, Olivier Silvie, Sabrina Marion, Ida Chiara Guerrera, Johannes T. Dessens, Christine Pierrot, Jamal Khalife

“…Our study shows that PbLRR1 is expressed during intra-erythrocytic development of the parasite, and up to the zygote stage in mosquitoes. PbLRR1 can be found in complex with PbPP1 in both asexual and sexual stages and inhibits its phosphatase activity. …”
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The formation and biological characterization of two allotriploid fish derived from interploid crosses by Jialin Cui, Mengxue Luo, Xin Gao, Hong Zhang, Xueyin Zhang, Li Ren, Shaojun Liu

“…The embryos of the two allotriploids developed normally through the zygote, cleavage, blastula, gastrula, segmentation and hatching stages, and high fertilization rates (3nR2C: 91.31%, 3nRC2: 84.09%) and hatching rates (3nR2C: 72.96%, 3nRC2: 71.15%) were observed. …”
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The activity of methylene blue against asexual and sexual stages of Plasmodium vivax by Camila Fabbri, Camila Fabbri, Glenda Quaresma Ramos, Glenda Quaresma Ramos, Glenda Quaresma Ramos, Djane Clarys Baia-da-Silva, Djane Clarys Baia-da-Silva, Djane Clarys Baia-da-Silva, Djane Clarys Baia-da-Silva, Djane Clarys Baia-da-Silva, Alexandre Oliveira Trindade, Luis Carlos Salazar-Alvarez, Juliana Costa Ferreira Neves, Juliana Costa Ferreira Neves, Ivanildes dos Santos Bastos, Ivanildes dos Santos Bastos, Allyson Guimarães Costa, Allyson Guimarães Costa, Allyson Guimarães Costa, Marcus Vinicius Guimarães Lacerda, Marcus Vinicius Guimarães Lacerda, Marcus Vinicius Guimarães Lacerda, Wuelton Marcelo Monteiro, Wuelton Marcelo Monteiro, Fabio Trindade Maranhão Costa, Stefanie Costa Pinto Lopes, Stefanie Costa Pinto Lopes, Stefanie Costa Pinto Lopes

“…An ex vivo schizont maturation assay, zygote to ookinete transformation assay, direct membrane feed assay (DMFA), and standard membrane feed assay (SMFA) using P. vivax gametocytes with MB exposure were performed. …”
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From TgO/GABA-AT, GABA, and T-263 Mutant to Conception of Toxoplasma by Joseph Lykins, Matthew J. Moschitto, Ying Zhou, Ekaterina V. Filippova, Hoang V. Le, Tadakimi Tomita, Barbara A. Fox, David J. Bzik, Chunlei Su, Seesandra V. Rajagopala, Kristin Flores, Furio Spano, Stuart Woods, Craig W. Roberts, Cong Hua, Kamal El Bissati, Kelsey M. Wheeler, Sarah Dovgin, Stephen P. Muench, Martin McPhillie, Colin W.G. Fishwick, Wayne F. Anderson, Patricia J. Lee, Mark Hickman, Louis M. Weiss, Jitender P. Dubey, Hernan A. Lorenzi, Richard B. Silverman, Rima L. McLeod

“…These candidates potentially influence merozoite’s capacity to make gametes that fuse to become zygotes, thereby contaminating environments and causing disease.…”
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Embryonic Development and Nutritional Requirements of Kutum Fry, Rutilus Frisii Kutum by Haghighi, Davoud Talebi

“…Seven period of embryogenesis were defined; I) the newly fertilized egg was zygote, occurred with 1 cell, with an average egg diameter of 2.21 mm, and mean weight of 9.99 mg and lasted for 15 min. …”
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7.345 The Science of Sperm, Fall 2014 by Lesch, Bluma (Bibi), George, Renee

“…Sperm are tiny, haploid cells with a supremely important job: They deliver the paternal genome to the egg, helping create a zygote that develops into a new individual. For a human male, however, only a small fraction of the sperm produced will ever fertilize an egg. …”
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Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers by Jagveer Singh, H S Dhaliwal, Anirudh Thakur, G S Sidhu, P Chhuneja, Kuldeep Pandey

“…The SSR analysis showed that only 85% multifoliate seedlings were zygotic while, the rest were not confirmed to be zygotic. …”
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Maternal and paternal genomes contribute equally to the transcriptome of early plant embryos by Nodine, Michael D., Bartel, David

“…However, other gene-expression studies and genetic analyses show that some transcripts must derive from the early zygotic genome7, 8, 9, 10, 11, 12, 13, 14, implying that the prevailing model does not fully explain the nature of zygotic genome activation in plants. …”
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Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation by Jue Zhang, Shuai-Bo Pi, Nan Zhang, Jing Guo, Wei Zheng, Lizhi Leng, Ge Lin, Heng-Yu Fan

“…It is unclear how translational efficiency and codon stringency affect zygotic genome activation and early embryonic development. …”
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Hybridization and hybrid speciation under global change by Vallejo-Marín, M, Hiscock, SJ

“…We thus speculate that global change will have a larger effect on eroding pre‐zygotic barriers (eco‐geographical isolation and phenology) than post‐zygotic barriers, shifting the relative importance of these two classes of reproductive barriers from what is usually seen in naturally produced hybrids where pre‐zygotic barriers are the largest contributors to reproductive isolation. …”