In vivo modulation of endogenous gene expression via CRISPR/Cas9-mediated 3’UTR editing by Kärt Mätlik, Soophie Olfat, Mark Cary Cowlishaw, Eva Domenech Moreno, Saara Ollila, Jaan-Olle Andressoo

“…We demonstrate that CRISPR/Cas9-mediated excision of a long inhibitory sequence from Gdnf native 3'UTR in mouse zygotes increases the levels of endogenous GDNF with similar phenotypic alterations in embryonic kidney development as we described in GDNF constitutive and conditional hypermorphic mice. …”
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Osteoblast Dysfunction in Non-Hereditary Sclerosing Bone Diseases by Liberato Giardullo, Alberto Altomare, Cinzia Rotondo, Addolorata Corrado, Francesco Paolo Cantatore

“…Protein kinases play also a leading role in triggering the activation of osteoblasts in this group of diseases. Post-zygotic changes in mitogen-activated protein kinase (MAPK) have been shown to be associated with sporadic cases of Melorheostosis. …”
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Seed Viability Test, Germination Rate and In vitro Regeneration of Some Alkanna Species by Cennet YAMAN, Serkan URANBEY, Hussein Abdullah Ahmed AHMED, Dilek BAŞALMA

“…orientalis and 5.0% for A. sieheana, no germination was observed in other applications. The highest zygotic shoot and root formation rate were obtained from A. orientalis with 91.6% and 40.3%, respectively. …”
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Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. by Roman V. Ignatenko, Olga V. Chirva, Maria A. Ershova, Natalia A. Galibina

“…In addition to the genotype effect, unsuitable nutrient medium or late developmental stages of zygotic embryos could be the reasons for the lack of callus induction. …”
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Somatic Embryogenesis in Spinach—A Review by Snežana Zdravković-Korać, Maja Belić, Dušica Ćalić, Jelena Milojević

“…Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. …”
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The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage by Fang Wang, David H. McCulloh, Kasey Chan, Ashley Wiltshire, Caroline McCaffrey, James A. Grifo, David L. Keefe

“…The telomere length of human blastocysts exceeds that of oocytes and telomerase activity increases after zygotic activation, peaking at the blastocyst stage. …”
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Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro by Annelies Raes, Eline Wydooghe, Krishna Chaitanya Pavani, Osvaldo Bogado Pascottini, Katleen Van Steendam, Maarten Dhaenens, Annekatrien Boel, Sonia Heras, Björn Heindryckx, Luc Peelman, Dieter Deforce, Filip Van Nieuwerburgh, Geert Opsomer, Ann Van Soom, Katrien Smits

“…The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. …”
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The ceramide synthase 2b gene mediates genomic sensing and regulation of sphingosine levels during zebrafish embryogenesis by Karen Mendelson, Suveg Pandey, Yu Hisano, Frank Carellini, Bhaskar C Das, Timothy Hla, Todd Evans

“…We show that sphk2 maternal-zygotic mutant zebrafish embryos (sphk2MZ) display early developmental phenotypes, including a delay in epiboly, depleted S1P levels, elevated levels of sphingosine, and resistance to sphingosine toxicity. …”
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Experimental Crossing Confirms Reproductive Isolation between Cryptic Species within <i>Eulimnogammarus verrucosus</i> (Crustacea: Amphipoda) from Lake Baikal by Polina Drozdova, Alexandra Saranchina, Ekaterina Madyarova, Anton Gurkov, Maxim Timofeyev

“…Experimental crossing of the western and southern species, which are morphologically indistinguishable and have adjacent ranges, showed their separation with a post-zygotic reproductive barrier, as hybrid embryos stopped developing roughly at the onset of gastrulation. …”
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Synthetic two-species allodiploid and three-species allotetraploid Saccharomyces hybrids with euploid (complete) parental subgenomes by Zsuzsa Antunovics, Adrienn Szabo, Lina Heistinger, Diethard Mattanovich, Matthias Sipiczki

“…These can mate with cells of a third species resulting in aneuploid zygotes having at least one incomplete subgenome. …”
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Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm by Jae-Wook Yoon, Seung-Eun Lee, Won-Jae Kim, Dae-Cheol Kim, Cheol-Ho Hyun, Shin-Ji Lee, Hyo-Jin Park, So-Hee Kim, Seung-Hwan Oh, Do-Geon Lee, Da-Bin Pyeon, Eun-Young Kim, Se-Pill Park

“…The rates of the normal formation of two pronuclei and total sperm penetration were the highest in zygotes fertilized with sperm from the JBC-A bull. …”
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Zebrafish Paralogs <i>brd2a</i> and <i>brd2b</i> Are Needed for Proper Circulatory, Excretory and Central Nervous System Formation and Act as Genetic Antagonists during Development by Gregory L. Branigan, Kelly S. Olsen, Isabella Burda, Matthew W. Haemmerle, Jason Ho, Alexandra Venuto, Nicholas D. D’Antonio, Ian E. Briggs, Angela J. DiBenedetto

“…Brd2 is a protooncogene and candidate gene for juvenile myoclonic epilepsy in humans, a homeobox gene regulator in Drosophila, and a maternal-zygotic factor and cell death modulator that is necessary for normal development of the vertebrate central nervous system (CNS). …”
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Parthenogenesis and the Evolution of Anisogamy by George W. A. Constable, Hanna Kokko

“…Here, equal contribution from both mating types to zygotes that are sufficiently well provisioned can outweigh the additional costs associated with syngamy.…”
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Comparison of leaf volatile aroma constituents and phenolic acid profiles of the seedling originated polyembryonic mango (Mangifera indica L.) genotypes by Nandkishor Kanade, Shivashankara K S, Kurian R M, Sankaran M

“…Such biochemical markers can also discriminate the nucellar and zygotic seedlings in polyembryonic mango varieties. …”
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Structure and function of Plasmodium actin II in the parasite mosquito stages. by Andrea J Lopez, Maria Andreadaki, Juha Vahokoski, Elena Deligianni, Lesley J Calder, Serena Camerini, Anika Freitag, Ulrich Bergmann, Peter B Rosenthal, Inga Sidén-Kiamos, Inari Kursula

“…We confirm expression in male gametocytes and zygotes and show that actin II is associated with the nucleus in both stages in filament-like structures. …”
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Production of viable bovine embryos by intracytoplasmic sperm injection of oocytes harvested from slaughtered old cows by Mihai Cenariu, Mihai Borzan, Sorin Dan, Remus Chiorean, Emoke Pall

“….; (3) Results: Fertilized oocytes transformed into 46 zygotes (fertilization rate of 59.74%), while after 168 h of cultivation 38 transferable compact morulae or early blastocysts were obtained; (4) Conclusions: Intracytoplasmic sperm injection can represent a viable alternative to classical IVF, when oocytes or sperm with lower fertility are used. …”
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Constructive connectomics: How neuronal axons get from here to there using gene-expression maps derived from their family trees. by Stan Kerstjens, Gabriela Michel, Rodney J Douglas

“…However, the limited information capacity of the zygotic genome puts a strong constraint on how, and which, axonal routes can be encoded. …”
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Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs by Hiroshi SUZUKI, Hiroyuki WATANABE, Yasuyuki ABE

“…Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock, and for the treatment of human infertility. …”
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Development of golden hamster embryos effectively produced by injection of sperm heads sonicated in Tris‐HCl buffer with EGTA by Nami Morishita, Masanori Ochi, Toshitaka Horiuchi

“…Embryonic development was improved following hamster oocyte injection with sperm heads sonicated in TBS + EGTA compared to in TCM199 (8‐cell: 84.1% vs 65.4%; morula: 78.4% vs 43.2%; blastocyst: 42.0% vs 17.3%). Gene expression of zygotic genome activation in 2‐cell embryos was significantly higher with TBS + EGTA than with TCM199. …”
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First successful pregnancies following embryo selection using Time-lapse technology in Iran: Case report by Azita Faramarzi, Mohammad Ali Khalili, Mehrdad Soleimani

“…After ovarian hyper stimulation, 6 COCs were retrieved and inseminated with 25,000 progressive sperms/ oocyte. Five zygotes were placed individually into the micro wells of equilibrated embryo scope dish for Time-lapse observation, and incubated at 37°C, 5% CO2. …”
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