Effect of sperm insemination duration on in vitro fertilisation (IVF) performance in goats by Abdullah, Ramli, Wan Khadijah, W.E., Tan, W.L.

“…After 8-14 hr or 1824 hr of insemination, in vitro culture (IVC) of presumptive zygotes were further incubated in CO 2 (5%) incubator at 38.5°C for preimplantation embryonic development. …”

Effects of chemical treatments on seed germination and desiccation of wild banana (Musa acuminata colla ssp malaccensis and truncata) seeds for cryopreservation by Mohlisun, Zaitialia

“…This option allowed the use of zygotic embryo for conservation of wild banana via cryopreservation. …”
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One-Step In Vitro Generation of ETV2-Null Pig Embryos by Marta Moya-Jódar, Giulia Coppiello, Juan Roberto Rodríguez-Madoz, Gloria Abizanda, Paula Barlabé, Amaia Vilas-Zornoza, Asier Ullate-Agote, Chiara Luongo, Ernesto Rodríguez-Tobón, Sergio Navarro-Serna, Evelyne París-Oller, Maria Oficialdegui, Xonia Carvajal-Vergara, Laura Ordovás, Felipe Prósper, Francisco Alberto García-Vázquez, Xabier L. Aranguren

“…Hence, here, we describe an efficient one-step method for the generation of hematoendothelial-disabled pig embryos via CRISPR-Cas9 microinjection in zygotes. This model could be used in experimentation related to the in vivo generation of humanized organs.…”
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Callus and somatic embryo induction in date palm cv. Khazravi using leaves, immature fruit and endosperm explants by Fateme Farhadi KolahKaj, Payam Pour Mohammadi, Mohammad Farkhari, Khalil0916 AlamiSaied

“…Also in endosperm with zygotic embryo culture, embryo development and the root and shoot were formed after three months. …”
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<i>Stipagrostis pennata</i> (Trin.) De Winter Artificial Seed Production and Seedlings Multiplication in Temporary Immersion Bioreactors by Masoumeh Asadi Aghbolaghi, Beata Dedicova, Farzad Sharifzadeh, Mansoor Omidi, Ulrika Egertsdotter

“…Experimental results also indicated that the total protein analyses of zygotic seedlings and seedlings originating from the synthetic seeds showed no statistically significant differences between these samples.…”
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Maternal Factor Dppa3 Activates 2C-Like Genes and Depresses DNA Methylation in Mouse Embryonic Stem Cells by Chuanyu Zhang, Hang Wen, Siying Liu, Enze Fu, Lu Yu, Shang Chen, Qingsheng Han, Zongjin Li, Zongjin Li, Na Liu, Na Liu

“…Mouse embryonic stem cells (ESCs) contain a rare cell population of “two-cell embryonic like” cells (2CLCs) that display similar features to those found in the two-cell (2C) embryo and thus represent an in vitro model for studying the progress of zygotic genome activation (ZGA). However, the positive regulator determinants of the 2CLCs’ conversion and ZGA have not been completely elucidated. …”
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Revisiting the Genetic, Taxonomic and Evolutionary Aspects of Chagas Disease Vectors of the <i>Triatoma phyllosoma</i> Subcomplex (Hemiptera, Triatominae) by Natália Regina Cesaretto, Yago Visinho dos Reis, Jader de Oliveira, Cleber Galvão, Kaio Cesar Chaboli Alevi

“…The phylogenetic reconstruction enabled us to rescue the six species as independent lineages. Post-zygotic reproductive isolation barriers (sterility and/or hybrid collapse) were observed for some experimental crosses. …”
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Transgenic mice Cre-dependently expressing mutant polymerase-gamma: novel test-system for pharmacological study of mitoprotective drugs by Marina V. Kubekina, Yulia Yu. Silaeva, Alexandra V. Bruter, Diana S. Korshunova, Leonid A. Ilchuk, Yulia D. Okulova, Mariya O. Soldatova, Evgeniya Seryogina, Inga M. Kolesnik, Polina A. Ukolova, Mikhail V. Korokin, Alexey V. Deykin

“…Materials and methods: Murine zygotes were microinjected with transgene construction carrying mutant murine Polg coding sequence and GFP coding sequence by a loxP-flanked STOP-cassette. …”
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Predicting distributions of Wolbachia strains through host ecological contact—Who's manipulating whom? by Clive T. Darwell, Daniel Souto‐Vilarós, Jan Michalek, Sotiria Boutsi, Brus Isua, Mentap Sisol, Thomas Kuyaiva, George Weiblen, Vlastimil Křivan, Vojtech Novotny, Simon T. Segar

“…We then account for post‐zygotic offspring mortality during CI mating, modeling fitness clines across developmental resources—the fecundity trade‐off hypothesis. …”
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Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency network by Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan, Bobby Cheng Peow Tan, Di Chen, Yun-Shen Chan, Huck Hui Ng, Wanlu Liu, Chih-Hung Hsu, Dan Zhang, Yang Shen, Hongqing Liang

“…Currently, extensive insights have been revealed for the totipotency regulatory network around the zygotic genome activation (ZGA) period. However, how the dissolution of the totipotency network is triggered to ensure the timely embryonic development following ZGA is largely unknown. …”
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Correction of Beta-Thalassemia IVS-II-654 Mutation in a Mouse Model Using Prime Editing by Haokun Zhang, Ruilin Sun, Jian Fei, Hongyan Chen, Daru Lu

“…We microinjected PE3 components (pegRNA, nick sgRNA, and PE2 mRNA) into the zygotes from IVS-II-654 mice to generate mutation-edited mice. …”
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Reproductive biology of two sympatric species of tooth-carps: Aphanius hormuzensis and Aphanius furcatus, from south of Iran (Teleostei: Aphaniidae) by Mojtaba Masoudi, Hamid Reza Esmaeili, Mehrgan Ebrahimi, Azad Teimori, Mahvash Seifali

“…Due to overlapping of spawning season in these two sympatric species, it seems that other pre- and post-zygotic factors are responsible for absence of natural hybrids in the studies tooth-carps in the Mehran River.…”
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Scd1 Deficiency in Early Embryos Affects Blastocyst ICM Formation through RPs-Mdm2-p53 Pathway by Huimin Niu, Anmin Lei, Huibin Tian, Weiwei Yao, Ying Liu, Cong Li, Xuetong An, Xiaoying Chen, Zhifei Zhang, Jiao Wu, Min Yang, Jiangtao Huang, Fei Cheng, Jianqing Zhao, Jinlian Hua, Shimin Liu, Jun Luo

“…Knockout <i>Scd1</i> gene by CRISPR/Cas9 from zygotes revealed a decrease in lipid droplets (LDs) and damage in the inner cell mass (ICM) formation of blastocyst. …”
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An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation by Yukiko U. Inoue, Yuki Morimoto, Mayumi Yamada, Ryosuke Kaneko, Kazumi Shimaoka, Shinji Oki, Mayuko Hotta, Junko Asami, Eriko Koike, Kei Hori, Mikio Hoshino, Itaru Imayoshi, Takayoshi Inoue

“…By microinjecting these donor DNAs along with CRISPR/Cas9 components into mouse zygotes, we have effectively generated fluorescent reporter lines and recombinase drivers. …”
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Loss of WTAP Impairs Early Parthenogenetic Embryo Development by Jindong Hao, Siyi Huang, Dongxu Wang, Yongxun Jin, Mingjun Zhang, Jiabao Zhang, Xianfeng Yu

“…To investigate the role of WTAP in porcine early embryonic development, si-WTAP was microinjected into porcine parthenogenetic zygotes. WTAP knockdown significantly reduced the blastocyst rate and global m⁶A levels, but did not affect the cleavage rate. …”
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m6A Profile Dynamics Indicates Regulation of Oyster Development by m6A-RNA Epitranscriptomes by Lorane Le Franc, Bruno Petton, Pascal Favrel, Guillaume Rivière

“…The putative epitranscriptome implication in the cleavage, maternal-to-zygotic transition, and cell differentiation in a lophotrochozoan model brings new insights into the control and evolution of developmental processes.…”
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The Oocyte-Specific Linker Histone H1FOO Is Not Essential for Mouse Oogenesis and Fertility by Fernando Sánchez-Sáez, Raquel Sainz-Urruela, Natalia Felipe-Medina, Yazmine B. Condezo, Manuel Sánchez-Martín, Elena Llano, Alberto M. Pendás

“…Histone H1 is a linker histone essential for packing the nucleosome into higher-order structures, and H1FOO (H1 histone family, member O, oocyte-specific) is a H1 variant whose expression pattern is restricted to growing oocytes and zygotes. To further explore the function of H1FOO, we generated mice lacking the <i>H1foo</i> gene by the CRISPR/Cas9 technique. …”
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Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization by Rafael Montalt, Laura Prósper, María Carmen Vives, Luis Navarro, Patrick Ollitrault, Pablo Aleza

“…Histological observations of pollen tube growth and seed production in self-pollinated flowers were used to evaluate the breakdown of SI, while the genetic analysis with SSR and SNP markers confirmed that all recovered plants were zygotic and had been originated by selfing. Our results confirm that the SI reaction can be surpassed by temperature stress, bud pollination and tetraploidy. …”
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Antioxidants Application Enhances Regeneration and Conversion of Date Palm (<i>Phoenix dactylifera</i> L.) Somatic Embryos by Amal F. M. Zein El Din, Rasmia S. S. Darwesh, Mohamed F. M. Ibrahim, Gehan M. Y. Salama, Ibrahim M. Shams El-Din, Walid B. Abdelaal, Ghada A. Ali, Maha S. Elsayed, Ismail A. Ismail, Eldessoky S. Dessoky, Yasmin M. R. Abdellatif

“…Many embryogenic systems have been designed to generate somatic embryos (SEs) with the morphology, biochemistry, and vigor uniformity of zygotic embryos (ZEs). During the current investigation, several antioxidants were added to the maturation media of the developing somatic embryos of date palm. …”
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Establishing an in vitro production program for buffalo embryos (Bubalus bubalis) in Colombia by Felipe Gamarra P, Viviana Rendón V, Aldemar Chávez R, Leonardo Perez S, Walter Cardona-Maya, Jesús Berdugo G

“…Frozen semen of seven Mediterranean bulls were used, motile sperm was obtained using the Percoll technique and oocytes were inseminated with 2 million sperm/ml. Presumptive zygotes were cultured for 6 days, grade 1 embryos obtained were frozen using ethylene glycol. …”
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