Production of Nematicidal Pinosylvin Stilbenes in Cell Suspension Cultures of <i>Pinus koraiensis</i> by Fungal Elicitation by Yi Rae Kim, Jung Yeon Han, Yong Eui Choi

“…Cell suspension cultures were established from the cultures of calli derived from mature zygotic embryos of <i>P. koraiensis</i> in 1/2 Litvay medium containing 2.2 μM 2,4-D and 2.2 μM BA. …”
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Physiological Responses and Gene Expression Patterns in Open-Pollinated Seedlings of a Pummelo-Mandarin Hybrid Rootstock Exposed to Salt Stress and Huanglongbing by Lamiaa M. Mahmoud, Patrick J. Huyck, Christopher I. Vincent, Frederick G. Gmitter, Jude W. Grosser, Manjul Dutt

“…However, it is necessary to propagate selected lines through tissue culture or cuttings because of the high percentage of zygotic seedlings derived from S10.…”
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Comparison of plastid genomes and ITS of two sister species in Gentiana and a discussion on potential threats for the endangered species from hybridization by Jiuyang Mao, Yuze Liang, Xue Wang, Dequan Zhang

“…Conclusion G. rigescens and G. cephalantha are recently diverged species which might not have undergone stable post-zygotic isolation. Though plastid genome shows obvious advantage in exploring phylogenetic relationships of some complicated genera, the intrinsic phylogeny was not revealed because of matrilineal inheritance here; nuclear genomes or regions are hence crucial for uncovering the truth. …”
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Oocyte Quality, In Vitro Fertilization and Embryo Development of Alpaca Oocytes Collected by Ultrasound-Guided Follicular Aspiration or from Slaughterhouse Ovaries by Leandra Landeo, Michele Zuñiga, Teddy Gastelu, Marino Artica, Jaime Ruiz, Mauricio Silva, Marcelo H. Ratto

“…Grade I to III COCs were in vitro matured for 26 h and in vitro fertilized afterwards for 20 h using fresh alpaca epididymal spermatozoa. Presumptive zygotes from both groups were in vitro cultured for 7 days. …”
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Resveratrol supplementation during in vitro maturation improves embryo development of prepubertal goat oocytes selected by brilliant cresyl blue staining by Anna-Rita PIRAS, Irene MENÉNDEZ-BLANCO, Sandra SOTO-HERAS, Maria-Gracia CATALÁ, Dolors IZQUIERDO, Luisa BOGLIOLO, Maria-Teresa PARAMIO

“…After 24 h, IVM-oocytes were fertilized with fresh semen and presumptive zygotes were in vitro cultured for 8 days. Oocytes were assessed for blastocyst development and quality, mitochondrial activity and distribution, and levels of GSH, ROS, and ATP. …”
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Cd248a and Cd248b in zebrafish participate in innate immune responses by Xianpeng Li, Ruitong Guo, Shuaiqi Yang, Xiangmin Zhang, Xiu Yin, Lei Teng, Shicui Zhang, Guangdong Ji, Hongyan Li, Hongyan Li

“…Both cd248a and cd248b exhibited similar and dynamic expression pattern in early development, both genes had weak maternal expression, the zygotic transcripts were first seen in anterior somites and head mesenchyme, then shifted to eyes and head mesenchyme, later expanded to branchial arches, and gradually declined with development. …”
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Assisted gamete treatment to pinpoint acquired meiotic maturity and overcome oocyte activation deficiency contributed by both gametes by Philip Xie, B.Sc., Ashley Aluko, M.D., Stephanie Cheung, M.Sc., Dan Goldschlag, M.D., Owen Davis, M.D., Zev Rosenwaks, M.D., Gianpiero D. Palermo, M.D., Ph.D.

“…To address this complex form of OAD, in couple 1, 27 oocytes out of 34 retrieved presented normal metaphase II spindles after EOM; ICSI with AGT yielded a fertilization rate of 63.0% (17/27). All 17 zygotes were cryopreserved initially. Two embryos were thawed and transferred, yielding a monochorionic diamniotic twin pregnancy. …”
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Small Noncoding RNA Signatures for Determining the Developmental Potential of an Embryo at the Morula Stage by Angelika Timofeeva, Yulia Drapkina, Ivan Fedorov, Vitaliy Chagovets, Nataliya Makarova, Maria Shamina, Elena Kalinina, Gennady Sukhikh

“…As part of the optimization of assisted reproductive technology programs, the aim of the study was to identify key small noncoding RNA (sncRNA) molecules that participate in maternal-to-zygotic transition and determine development potential and competence to form a healthy fetus. …”
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PfSRPK1 Regulates Asexual Blood Stage Schizogony and Is Essential for Male Gamete Formation by Sudhir Kumar, Vinay K. Baranwal, Amanda S. Leeb, Meseret T. Haile, Kenza M. Z. Oualim, Nina Hertoghs, Ashley M. Vaughan, Stefan H. I. Kappe

“…Gametocytes taken up in an infectious blood meal by female anopheline mosquito get activated to form gametes and fuse to form short-lived zygotes, which transform into ookinetes to infect mosquitoes. …”
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Variants in Maternal Effect Genes and Relaxed Imprinting Control in a Special Placental Mesenchymal Dysplasia Case with Mild Trophoblast Hyperplasia by Tien-Chi Huang, Kung-Chao Chang, Jen-Yun Chang, Yi-Shan Tsai, Yao-Jong Yang, Wei-Chun Chang, Chu-Fan Mo, Pei-Hsiu Yu, Chun-Ting Chiang, Shau-Ping Lin, Pao-Lin Kuo

“…Finally, we identified heterozygous mutations in the X-linked <i>ATRX</i> gene, a known maternal–zygotic imprinting regulator in the patient. Overall, our study demonstrates that PMD and PHM may share overlapping etiologies with the defective/relaxed dosage control of imprinted genes, representing two extreme ends of a spectrum.…”
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Characterization of SHARPIN knockout Syrian hamsters developed using CRISPR/Cas9 system by Jinxin Miao, Tianfeng Lan, Haoran Guo, Jianyao Wang, Guangtao Zhang, Zheng Wang, Panpan Yang, Haoze Li, Chunyang Zhang, Yaohe Wang, Xiu‐Min Li, Mingsan Miao

“…Methods A single‐guide ribonucleic acid targeting exon 1 of SHARPIN gene was designed and constructed. The zygotes generated by cytoplasmic injection of the Cas9/gRNA ribonucleoprotein were transferred into pseudopregnant hamsters. …”
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#71 : Successful IVF Procedure on SARS-CoV-2 Positive Advance Maternal Age Woman by Novita Prasetiawati, Agus Supriyadi, Hadi Sjarbaini, Gde Suardana, Sudirmanto Tarigan, Mohammad Haekal, Deana Rosaria Indah, Gangsar Pariyanti, Anom Bowolaksono

“…The oocytes were obtained from the follicular fluid analysis, following intracytoplasmic sperm injection. The zygotes were cultured for 48 hours and tested negative for SARS-CoV-2. …”
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In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (<i>Eruca sativa</i> Mill.) Variety Domaća Rukola by Nevena Banjac, Dijana Krstić-Milošević, Tatjana Mijalković, Mirjana Petrović, Tatjana Ćosić, Mariana Stanišić, Branka Vinterhalter

“…Somatic embryogenesis from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar. …”
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The ERA-Related GTPase AtERG2 Associated with Mitochondria 18S RNA Is Essential for Early Embryo Development in Arabidopsis by Pengyu Cheng, Hongjuan Li, Linlin Yuan, Huiyong Li, Lele Xi, Junjie Zhang, Jin Liu, Yingdian Wang, Heping Zhao, Huixin Zhao, Shengcheng Han

“…The T-DNA insertion lines of AtERG2 showed silique shortage, early seed abortion, and sporophytic maternal effects (SME), in which some seeds arrested in the zygotic stage at 1.5 days after pollination (DAP) and aborted at 2.0 DAP in aterg2-1 +/−. …”
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Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene by Mohammad Shamim Hossein, Young-Bum Son, Young-Bum Son, Yeon Woo Jeong, Yeon Woo Jeong, Yeon Ik Jeong, Mi Na Kang, Eun Ji Choi, Kang Bae Park, Yu Ra Bae, Dae Young Kim, Woo Suk Hwang, Woo Suk Hwang

“…In Experiment II, a total of 59, 70, and 65 presumptive zygotes produced by fresh, Percoll gradient, and swim-up methods, respectively, were transferred to surrogates (5 for each group); the Percoll gradient (27.27%) and swim-up samples (14.29%) showed the highest blastocyst formation rates, while fresh diluted semen did not produce any blastocyst. …”
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CRISPR/Cas9-editing of KISS1 to generate pigs with hypogonadotropic hypogonadism as a castration free trait by Julio M. Flórez, Julio M. Flórez, Kyra Martins, Staci Solin, Jonathan R. Bostrom, Paula Rodríguez-Villamil, Felipe Ongaratto, Sabreena A. Larson, Uyanga Ganbaatar, Alexander W. Coutts, Doug Kern, Thomas W. Murphy, Eui-Soo Kim, Daniel F. Carlson, Abe Huisman, Tad S. Sonstegard, Clay A. Lents

“…Genome editors were intracytoplasmically injected into 684 swine zygotes and transferred to 19 hormonally synchronized surrogate sows. …”
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Truncation of IFT80 causes early embryonic loss in Holstein cattle associated with Holstein haplotype 2 by M. Sofía Ortega, Derek M. Bickhart, Kelsey N. Lockhart, Daniel J. Null, Jana L. Hutchison, Jennifer C. McClure, John B. Cole

“…Abattoir-derived oocytes were fertilized in vitro, and zygotes were injected at the one-cell stage either with a guide RNA and CAS9 mRNA complex (n = 100) or Cas9 mRNA (control, n = 100) before return to culture, and replicated 3 times. …”
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Plant Regeneration via Somatic Embryogenesis in <i>Larix principis-rupprechtii</i> Mayr by Shuaifei Jiang, Xiaoyi Chen, Ying Gao, Ying Cui, Lisheng Kong, Jian Zhao, Jinfeng Zhang

“…In this study, we described a high-efficiency SE system via indirect pathways and investigated the effect of genotype, culture conditions and phytohormones on SE. Immature zygotic embryos (IZEs) of <i>L. principis-rupprechtii</i> Mayr were used as explant materials. …”
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Overlaps in olfactive signalling coupled with geographic variation may result in localised pollinator sharing between closely related Ficus species by Xiaoxia Deng, Yufen Cheng, Yan-Qiong Peng, Hui Yu, Magali Proffit, Finn Kjellberg

“…In Ficus, odours of receptive figs differ among species and the specific pollinators generally only enter figs of their host species ensuring a pre-zygotic barrier to plant interspecific hybridisation. …”
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p66Shc in sheep preimplantation embryos: Expression and regulation of oxidative stress through the manganese superoxide dismutase-reactive oxygen species metabolic pathway by Tong Zhang, Jiaxin Zhang, Ruilan Li

“…RNAi-mediated silencing of p66Shc via microinjection of p66Shc siRNA into sheep zygotes resulted in significant decreases in p66Shc mRNA and protein levels (p<0.05). …”
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