Non-Invasive Metabolic Live Cell Imaging of Early Embryo Development Using Novel Adapted Confocal Microscopy in Ageing by Fabrizzio HORTA, Hope NEWMAN, Erick J. VARGAS-ORDAZ, Victor J. CADARSO, Reza NOSRATI, Adrian NEILD, Beverly VOLLENHOVEN, Sally CATT

“…Embryos from young and old females showed significantly different NADH levels during embryo development (Young: 29,889 ±864AU; Old: 25,972 ±260AU; p[Formula: see text]0.0001). Zygotes that reached the blastocyst stage in old females had significantly decreased NADH levels compared to young females (Young: 31,508 ±853AU, Old: 26,417 ±186AU; p=0.0002). …”
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Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation by H. Güralp, K. Pocherniaieva, M. Blecha, T. Policar, M. Pšenička, T. Saito

“…Our results indicate that zygotic gene expression (MBT) starts after this stage. …”
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Outcomes of assisted reproductive technologies in discordant couples with HIV infection in a woman by Helen V. Mituyrina, Svetlana G. Perminova, Elena S. Sannikova

“…In the main group, a significantly lower number of oocyte-cumulus complexes [6.5 (3; 12) and 9 (6; 15); p0.001], zygotes [4 (2; 8) and 6 (4; 10); p0.001] and blastocysts [2 (0; 2) and 4 (1; 6); p0.001] were obtained in comparison with the control group. …”
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Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development by Tianqing Zhu, Jiwen Hu, Xiaowei Yang, Lisheng Kong, Juanjuan Ling, Junhui Wang, Sanping An

“…The relative transcript levels of these genes were measured in endosperm and zygotic and somatic embryos at different developmental stages. …”
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TOP2A deficiency leads to human recurrent spontaneous abortion and growth retardation of mouse pre-implantation embryos by Yuhan Duan, Huijia Fu, Jiayu Huang, Nanlin Yin, Linhong Liu, Xiru Liu

“…Moreover, in vivo experiments confirmed that inhibition of TOP2A impairs trophectoderm differentiation, embryonic mitochondrial function as well as the developmental rate; however, no differences were noted in the expression of zygotic genome activation-related genes. Conclusions Collectively, our data suggest that lower TOP2A expression is related to RSA as it inhibits trophoblast cell proliferation, migration, and invasion by activation of the FOXO signalling pathway. …”
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Estradiol Decline Before hCG Administration in COH Has a Negative Effect on IVF Outcomes in Patients Without OC Pretreatment by Zhu Y, Zheng Z, Fan B, Sun Y, Zhai J, Du Y

“…Based on pretreatment strategy, the two groups were further categorized into oral contraceptive (OC) and non-OC sub-groups, and IVF and clinical outcomes were compared between paired groups.Results: Number of dominant follicles on hCG day and normally fertilized zygotes were significantly decreased in E2 decline group, and the significantly reduced live birth rate in E2 decline group indicated the close relationship between E2 decline and clinical outcomes. …”
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Variation of Acid Phosphatase Activity in the Fallopian Tube of Rabbits: A Semiquantitative Histochemical Assessment by Virendra Kumar, Geetika Mohan, Niyati Airan, Aditi Srivastava, Anil Kumar Dwivedi

“…It is important for the passage of gametes, zygotes, and early embryos. Fallopian tube undergoes histochemical variations with ovarian cycles. …”
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High-throughput sequencing of RNA 5'- and 3'-termini yields insights into viral and vertebrate gene expression by Koppstein, David N. P. (David Neal Pira)

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Regulation of early cell fate specification in the crustacean embryo, Parhyale hawaiensis by Wen, T-C

“…These early asymmetries must translate into differential regulation of maternal to zygotic transition (MZT) with different germ layers to ensure correct specification and patterning during development. …”

Attenuated glucose uptake promotes catabolic metabolism through activated AMPK signaling and impaired insulin signaling in zebrafish by Longwei Xi, Longwei Xi, Gang Zhai, Gang Zhai, Yulong Liu, Yulong Liu, Yulong Gong, Qisheng Lu, Qisheng Lu, Zhimin Zhang, Haokun Liu, Haokun Liu, Junyan Jin, Junyan Jin, Xiaoming Zhu, Xiaoming Zhu, Zhan Yin, Zhan Yin, Zhan Yin, Zhan Yin, Shouqi Xie, Shouqi Xie, Shouqi Xie, Dong Han, Dong Han, Dong Han

“…Approxiamately 30% of glut2−/− fish survived to adulthood and could reproduce. The maternal zygotic mutant glut2 (MZglut2) fish exhibited growth retardation, decreased blood and tissue glucose levels, and low locomotion activity. …”
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One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses by Mengyu Gao, YuTing He, XingLong Zhu, WanLiu Peng, YanYan Zhou, Yang Deng, Guangneng Liao, Wei Ni, Yi Li, Jun Gao, Hong Bu, Jiayin Yang, Guang Yang, Yang Yang, Ji Bao

“…The feasibility of this one-step method to produce gene-edited pigs using rAAV-CRISPR/Cas9 oviductal injection into sows within 24 h of conception was then validated.Results: Our research firstly establishes the efficient delivery of CRISPR/Cas9 to pig zygotes, both in vivo and in vitro, using rAAV6. Successful gene editing in pigs was achieved through oviductal injection of rAAV-CRISPR/Cas9.Conclusion: This method circumvents the intricate procedures involved in in vitro embryo manipulation and embryo transfers, providing a straightforward and cost-effective approach for the production of gene-edited pigs.…”
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Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes by Desmond A. R. Tutt, Desmond A. R. Tutt, Gizem Guven-Ates, Gizem Guven-Ates, Wing Yee Kwong, Wing Yee Kwong, Rob Simmons, Fei Sang, Giuseppe Silvestri, Carla Canedo-Ribeiro, Alan H. Handyside, Remi Labrecque, Marc-André Sirard, Richard D. Emes, Richard D. Emes, Darren K. Griffin, Kevin D. Sinclair, Kevin D. Sinclair

“…Following IVM and IVF, putative zygotes were cultured to Day 8 in standard media. …”
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Ovule Development and <i>in Planta</i> Transformation of <i>Paphiopedilum</i> Maudiae by <i>Agrobacterium</i>-Mediated Ovary-Injection by Bai-Xue Luo, Li Zhang, Feng Zheng, Kun-Lin Wu, Lin Li, Xin-Hua Zhang, Guo-Hua Ma, Jaime A. Teixeira da Silva, Lin Fang, Song-Jun Zeng

“…The time taken for the embryo to develop to a mature sac was 45-50 days after pollination (DAP) and most mature embryo sacs had completed fertilization and formed zygotes by about 50–54 DAP. <i>In planta</i> transformation was achieved by injection of the ovaries by <i>Agrobacterium</i>, resulting in 38 protocorms or seedlings after several rounds of hygromycin selection, corresponding to 2, 7, 5, 1, 3, 4, 9, and 7 plantlets from <i>Agrobacterium</i>-mediated ovary-injection at 30, 35, 42, 43, 45, 48, 50, and 53 DAP, respectively. …”
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Assessment of sperm cryopreservatives and oocyte vitrification for In vitro embryo production in cattle by Ghareeb, Salman Hammadi

“…For in vitro culture (IVC) presumptive zygotes were vortexed and washed three times in HEPES-buffered TCM199 containing 0.4 g/l BSA and 4 μg/ml gentamicin, to remove cumulus cells and attached spermatozoa. …”
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Chromosome Doubling in Genetically Diverse Bilberry (<i>Vaccinium myrtillus</i> L.) Accessions and Evaluation of Tetraploids in Terms of Phenotype and Ability to Cross with Highbus... by Malgorzata Podwyszynska, Katarzyna Mynett, Monika Markiewicz, Stanisław Pluta, Agnieszka Marasek-Ciolakowska

“…To expand the gene pool and introduce new traits to the tetraploid cultivars of <i>Vaccinium corymbosum</i> from wild diploid species <i>V. myrtillus</i>, it is necessary to double the chromosome number in diploid species in order to overcome a post zygotic crossing barrier and a strong triploid block, existing within the genus <i>Vaccinium</i>. …”
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Modeling of asymmetric division of somatic cell in protoplasts culture of higher plants by S. I. Kondratenko, T. P. Pasternak, O. P. Samovol, O. M. Mogilna, O. V. Sergienko

“…Meanwhile, in all analyzed species, there was observed the division of the initial genetically homogeneous population of mitotically active cells into two types of asymmetric division: by the type of division of the mother cell into smaller daughter cells and by the type of the first asymmetric division of the zygotic embryo in planta. In this case, the first type of asymmetric division occurred during unusual cytomorphism of the mother cells: a pronounced heart-shaped form even before the first division, which is inherent in the morphology of somatic plant embryo in vitro at the heart-shaped stage. …”
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Single-Cell RNA Sequencing Reveals Differences in Chromatin Remodeling and Energy Metabolism among In Vivo-Developed, In Vitro-Fertilized, and Parthenogenetically Activated Embryos... by Jianlin Fan, Chang Liu, Yunjing Zhao, Qianqian Xu, Zhi Yin, Zhonghua Liu, Yanshuang Mu

“…We analyzed single-cell RNA-seq data from the oocyte to eight-cell stages in IVV, IVF, and PA porcine embryos, focusing on developmental differences during early zygotic genome activation (ZGA), a vital stage for embryonic development. (1) Our findings reveal that in vitro embryos (IVF and PA) exhibit more similar developmental trajectories compared to IVV embryos, with PA embryos showing the least gene diversity at each stage. (2) Significant differences in maternal mRNA, particularly affecting mRNA splicing, energy metabolism, and chromatin remodeling, were observed. …”
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Indirect somatic embryogenesis of Piper hispidinervum L. and evaluation of the regenerated plants by flow cytometry by Paulo Cesar Alves de Sousa, Stênio Steferson Silva e Souza, Gabriela Ferreira Nogueira, Inaê Mariê de Araújo Silva-Cardoso, Jonny Everson Scherwinski-Pereira

“…The somatic embryos-like structures of P. hispidinervum have conspicuous morphoanatomical similarities with the zygotic embryo, and flow cytometry analysis showed no significant variation in nuclear DNA size among plants regenerated in vitro and plants coming from seed germination, which indicates ploidy level stability. …”
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Implications des progrès récents en physiologie de la reproduction pour la conduite de la reproduction dans l’espèce caprine by P. CHEMINEAU, G. BARIL, B. LEBOEUF, M.C. MAUREL, F. ROY, M. PELLICER-RUBIO, B. MALPAUX, Y. COGNIE

“…La production in vitro d’embryons permet des taux élevés de développement après maturation et fécondation in vitro des ovocytes et pourrait permettre l’obtention de populations synchrones de zygotes au stade unicellulaire, nécessaire à l’application des biotechnologies. …”
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IGF2 reduces meiotic defects in oocytes from obese mice and improves embryonic developmental competency by Yanling Wan, Tahir Muhammad, Tao Huang, Yue Lv, Qianqian Sha, Shuang Yang, Gang Lu, Wai-yee Chan, Jinlong Ma, Hongbin Liu

“…Additionally, the embryonic developmental competency and embryos quality of IGF2-treated zygotes were also determined. Results In our findings, we observed significantly reduced contents of insulin-like growth factor 2 (IGF2) in the serum and oocytes of obese mice. …”
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