Does extreme asymmetric dominance promote hybridization between Anopheles coluzzii and Anopheles gambiae s.s. in seasonal malaria mosquito communities of West Africa? by Abdoulaye Niang, Patric S. Epopa, Simon P. Sawadogo, Hamidou Maïga, Lassana Konaté, Ousmane Faye, Roch K. Dabiré, Frédéric Tripet, Abdoulaye Diabaté

“…These recently-diverged sibling species do not exhibit intrinsic post-zygotic barriers to reproduction and are thought to be separated by strong assortative mating combined with selection against hybrids. …”
Get full text

A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9 by Yuqiang Qian, Di Wang, Wenchao Niu, Ding Zhao, Jinze Li, Zhiquan Liu, Xun Gao, Yang Han, Liangxue Lai, Zhanjun Li

“…Furthermore, we also successfully edited the genome of mouse embryos by microinjecting mRNA and sgRNA of sABE system into zygotes. Conclusions We have developed a substantially smaller sABE system that expands the targeting scope and offers higher precision of genome editing. …”
Get full text

Characterization of Creb-regulated transcription coactivator 1 (Crtc1) during embryogenesis of Zebrafish (Danio rerio) by Sie, Lawrence Eng Kean

“…I have characterized the spatial and temporal expression of crtc1 and crtc1b during zebrafish development, and identified unique splicing of maternal and zygotic specific crtc1b transcripts. Also, I have demonstrated the co-activator function of zebrafish Crtc1 and Crtc1b by using CRE luciferase reporter assay in cell culture. …”
Get full text

Histological and biochemical characterization of oil palm (Elaeis guineensis jacq.) endosperm during fruit development by Kok, Sau Yee

“…On the whole, the anatomical and nutritional study of oil palm endosperm has provided information on cellular changes and nutrient requirements for zygotic embryo development. This knowledge will be useful in optimizing the tissue culture media of oil palm in order to improve the efficiency of somatic embryogenesis.…”
Get full text

Somatic Embryogenesis and Organogenesis in Teak (Tectona Grandis L.) by Lim, Sooi Ping

“…In the second study, proliferation of shoots was achieved with four types of explants namely shoot tip, node, cotyledon and zygotic embryo. The embryo explant was found to produce the highest number of shoots with an average of 9.3 shoots (76% response) per explant on MS medium with 2.0mgl-1 BAP and 0.01mgl-1 NAA. …”
Get full text

Isolation and Characterization of MYB-Related Genes from Oil Palm (Elaeis Guineensis Jacq.) by Teoh, Wan Chin

“…OpMyb24, which contains a full-length coding region, was obtained from an oil palm zygotic embryo (OPZE) cDNA library. Its Myb-domain shows extensive homology to the snapdragon proteins Myb308 and Myb330, and maize protein Zm3 8, and these myb-like genes have been found to be involved in regulating phenylpropanoid and lignin biosynthesis, and control of flavonoid biosynthesis. …”
Get full text

Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows by Idil Serbetci, L. Antonio González-Grajales, Carolina Herrera, Iulian Ibanescu, Muhittin Tekin, Manuel Melean, Fumie Magata, Eleni Malama, Heinrich Bollwein, Dragos Scarlet, Dragos Scarlet

“…Presence of disease did not affect the time required by zygotes to reach specific developmental stages, as recorded by means of time-lapse monitoring. …”
Get full text

Multiple congenital malformations arise from somatic mosaicism for constitutively active Pik3ca signaling by Elise Marechal, Anne Poliard, Anne Poliard, Kilian Henry, Mathias Moreno, Mathilde Legrix, Nicolas Macagno, Grégoire Mondielli, Teddy Fauquier, Anne Barlier, Anne Barlier, Heather C. Etchevers

“…To further examine their development, we drove the most common post-zygotic activating mutation of Pik3ca in neural crest and related embryonic lineages. …”
Get full text

Potencial de obtenção de novos porta-enxertos em cruzamentos envolvendo limoeiro 'Cravo', laranjeira 'Azeda', tangerineira 'Sunki' e híbridos de Poncirus Trifoliata Potential of ob... by Walter dos Santos Soares Filho, Carlos Alberto da Silva ledo, Antônio da Silva Souza, Orlando Sampaio Passos, Matheus Pires Quintela, Lorenna Alves Mattos

“…Parameters evaluated were: fruit set, percentage of hybrids out of the total number of seedlings (zygotes and nucellars), stem height and diameter of the hybrids at 12 months of age. …”
Get full text

Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC class II genes upon Staphylococcus aureus pneumonia by Feng Li, Bowen Niu, Lingling Liu, Mengmin Zhu, Hua Yang, Boyin Qin, Xiuhua Peng, Lixiang Chen, Chunhua Xu, Xiaohui Zhou

“…Methods In this study, we have produced HLA DP401 and HLA DRA0101 humanized mice by microinjection of C57BL/6J zygotes. Neo‐floxed IAβ+/− mice were crossbred with Ella‐Cre and further crossbred with HLA DP401 or HLA‐DRA0101 humanized mice. …”
Get full text

Modification of <i>i</i>-GONAD Suitable for Production of Genome-Edited C57BL/6 Inbred Mouse Strain by Yukari Kobayashi, Takuya Aoshima, Ryota Ito, Ryota Shinmura, Masato Ohtsuka, Eri Akasaka, Masahiro Sato, Shuji Takabayashi

“…Improved genome editing via oviductal nucleic acid delivery (<i>i</i>-GONAD) is a novel method for producing genome-edited mice in the absence of ex vivo handling of zygotes. <i>i</i>-GONAD involves the intraoviductal injection of clustered regularly interspaced short palindromic repeats (CRISPR) ribonucleoproteins via the oviductal wall of pregnant females at 0.7 days post-coitum, followed by in vivo electroporation (EP). …”
Get full text

<i>Xenopus gpx3</i> Mediates Posterior Development by Regulating Cell Death during Embryogenesis by Hongchan Lee, Tayaba Ismail, Youni Kim, Shinhyeok Chae, Hong-Yeoul Ryu, Dong-Seok Lee, Taeg Kyu Kwon, Tae Joo Park, Taejoon Kwon, Hyun-Shik Lee

“…Using reverse transcription polymerase chain reaction (RT-PCR), we demonstrated the zygotic nature of this gene. Interestingly, the expression of <i>gpx3</i> enhanced during the tailbud stage of development, and whole mount in situ hybridization (WISH) analysis revealed <i>gpx3</i> localization in prospective tail region of developing embryo. …”
Get full text

Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality by Cláudia Lima Verde Leal, Karina Cañón-Beltrán, Yulia N. Cajas, Meriem Hamdi, Aracelli Yaryes, María Gemma Millán de la Blanca, Paula Beltrán-Breña, Rosane Mazzarella, Juliano Coelho da Silveira, Alfonso Gutiérrez-Adán, Encina M González, Dimitrios Rizos

“…This study aimed to evaluate the effect of extracellular vesicles (EVs) isolated from oviductal (OF) and uterine fluid (UF) in sequential IVC on the development and quality of bovine embryos. Zygotes were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum (dFCS) in the presence (BSA-EV and dFCS-EV) or absence of EVs from OF (D1 to D4) and UF (D5 to D8), mimicking in vivo conditions. …”
Get full text

Integrated transcriptomic and metabolic analyses provide insights into the maintenance of embryogenic potential and the biosynthesis of phenolic acids and flavonoids involving tran... by Junchen Wang, Junchen Wang, Lifeng Zhang, Lifeng Zhang, Liwang Qi, Liwang Qi, Shougong Zhang, Shougong Zhang

“…In this study, integrated transcriptomic and metabolomic analyses were performed in embryogenic callus (EC) and NEC originating from a single immature zygotic embryo to better decipher the key molecular and metabolic mechanisms required for embryogenic potential maintenance. …”
Get full text

Risk Factors for Early and Late Onset Preeclampsia in Reunion Island: Multivariate Analysis of Singleton and Twin Pregnancies. A 20-Year Population-Based Cohort of 2120 Preeclampsi... by Pierre-Yves Robillard, Malik Boukerrou, Gustaaf Dekker, Marco Scioscia, Francesco Bonsante, Brahim Boumahni, Silvia Iacobelli

“…Conclusions: Maternal ages over 35 years, chronic hypertension, history of preeclampsia, ovulation induction, in vitro fertilizations, history of perinatal deaths and twin pregnancy (in our experience, especially mono zygotic twin pregnancies) are significant risk factors for EOP. …”
Get full text

Establishment of a Wisent (<i>Bison bonasus</i>) Germplasm Bank by Anna Maria Duszewska, Magdalena Baraniewicz-Kołek, Jarosław Wojdan, Katarzyna Barłowska, Wojciech Bielecki, Paweł Gręda, Wojciech Niżański, Wanda Olech

“…After IVM, COCs were fertilized in vitro with wisent sperm. The obtained wisent zygotes, based on oocytes matured for 24 h and 30 h, were cultured for 216 h. …”
Get full text

C57BL/6J and B6129F1 Embryo Transfer: Unilateral and Bilateral Transfer, Embryo Number and Recipient Female Background Control for the Optimization of Embryo Survival and Litter Si... by Sofia Lamas, Filipa Franquinho, Marlene Morgado, João R. Mesquita, Fátima Gärtner, Irina Amorim

“…To explore this theme, 2-cell C57BL/6J embryos were transferred after overnight culture of freshly collected zygotes using different conditions: unilateral transfers using a total of 6, 8, 12, 15, 20 and 25 embryos were performed initially; then, this strain was also used for bilateral transfers using a total of 6, 12 and 20 embryos equally divided by the two oviducts. …”
Get full text

Micropropagation of Tongkat Ali (Eurycoma Longifolia Jack) Via Somatic Embryogenesis and Direct Plant Regeneration Techniques by Hussein, Sobri

“…In attempts to establish the somatic embryos of E. longifolia, the potential of cotyledon, zygotic embryo, leaf, petiole, stem and taproot in forming embryogenic callus were examined in the basal Murashige and Skoog (MS) medium supplemented with different auxins at various concentrations. …”
Get full text

Callus Induction from Explants of Eurycoma Longifolia Jack (Tongkat Ali) and the Production of 9- Methoxycanthin-6-One from the Induced Callus by Noormi, Rosli

“…Qualitative analysis using TLC revealed that 9-methoxycanthin-6-one was present in leaf, petiole, stem, rachis, tap root, fibrous root, cotyledon and zygotic embryo of intact plant. The quantitative analysis using HPLC showed that the highest concentration of 9-methoxycanthin-6-one content was found in tap root. …”
Get full text

The zebrafish as a model system for analyzing mammalian and native α-crystallin promoter function by Mason Posner, Kelly L. Murray, Matthew S. McDonald, Hayden Eighinger, Brandon Andrew, Amy Drossman, Zachary Haley, Justin Nussbaum, Larry L. David, Kirsten J. Lampi

“…We injected a variety of α-crystallin promoter fragments from each species combined with the coding sequence for green fluorescent protein (GFP) into zebrafish zygotes to determine the resulting spatiotemporal expression patterns in the developing embryo. …”
Get full text