Impacts of the COVID-19 pandemic on Chinese assisted reproductive technology institutions and human sperm banks: reflections in the post-pandemic era by Lun Wei, Jiakai Zhang, Xiaoling Deng, Chao Luo, Le Bo, Shasha Gao, Fei Qian, Shucheng Lu, Caiping Mao

“…In addition, couples should be reminded that during ART treatment, disinfectants should not be used excessively to minimize risks of damaging the reproductive system, gametes and zygotes. At the same time, timely and reasonable guidance for tackling negative emotions from stress response is needed to provide reassurance and to avoid irrational fear and excessive stress. …”
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MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway by Karina Cañón-Beltrán, Yulia N Cajas, Vasileios Almpanis, Sandra Guisado Egido, Alfonso Gutierrez-Adan, Encina M González, Dimitrios Rizos

“…Our aim was to verify whether miR-148b is taken up by embryos through gymnosis, validate its target genes, and investigate the effect of miR-148b supplementation on early embryo development and quality. Methods Zygotes were cultured in SOF + 0.3% BSA (Control) or supplemented with: 1 µM miR-148b mimics during: D1-D7 (miR148b) or D1-D4 (miR148b-OV: representing miRNA effect in the oviduct) or D4-D7 (miR148b-UT: representing miRNA effect in the uterus) or 1 µM control mimics was used during: D1-D7 (CMimic). …”
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Establishment of tissue culture and transformation protocol for tropical corn (Zea mays L.) inbred lines by Matazu, Nasiruddeen Umar

“…The lowest callus induction and proliferation frequency was recorded in CML 425 with 41.67% using 17 days after pollination (DAP) immature zygotic embryo (IZE). Addition of 0.015 mg/L AgNO3, 0.02 mg/L Ag2SO4 and 4.12 g/L -aminobutyric acid to culture media have significantly increased the formation of embryogenic callus. …”
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Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver by Arne Hinrichs, Barbara Kessler, Mayuko Kurome, Andreas Blutke, Elisabeth Kemter, Maren Bernau, Armin M. Scholz, Birgit Rathkolb, Simone Renner, Sebastian Bultmann, Heinrich Leonhardt, Martin Hrabĕ de Angelis, Hiroshi Nagashima, Andreas Hoeflich, Werner F. Blum, Martin Bidlingmaier, Rüdiger Wanke, Maik Dahlhoff, Eckhard Wolf

“…Methods: CRISPR/Cas9 technology was applied to mutate exon 3 of the GHR gene in porcine zygotes. Two heterozygous founder sows with a 1-bp or 7-bp insertion in GHR exon 3 were obtained, and their heterozygous F1 offspring were intercrossed to produce GHR-KO, heterozygous GHR mutant, and wild-type pigs. …”
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Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization by A. Suma, K. Joseph John, Kangila Venkataramana Bhat, Madhavan Latha, Chakkamadathil Jayasundaran Lakshmi, Mottaiyan Pitchaimuthu, V. A. M. Nissar, Polavakkalipalayam Palanisamy Thirumalaisamy, Chitra Devi Pandey, Sushil Pandey, Ashok Kumar, Raj Kumar Gautam, Gyanendra Pratap Singh

“…Presence of pre- and post-zygotic barriers to interspecific geneflow, differences in ploidy levels and genotype specific variations in chromosome numbers led to varying degrees of sterility in F1 plants of interspecific crosses. …”
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Dynamic patterning of maternal mRNAs in the Early C. elegans embryo by Li, Jialing, Ph. D. Massachusetts Institute of Technology

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Generation, proliferation and expansion of epithelial cells from primary tissue into mucosoid cultures by Boccellato, F, Meyer, TF

“…The stimulus can be of various sources including physical stimulation (e.g., heat, electromagnetic radiation, contact and others) chemical stimulation (e.g. natural or synthetic molecules) or biological stimulation (e.g., whole or part of organisms such as gamete, zygotes, embryos, bacteria, viruses or parasites). …”

Fertility in seasonal-calving pasture-based lactating dairy cows following timed artificial insemination or timed embryo transfer with fresh or frozen in vitro–produced embryos by A.D. Crowe, J.M. Sánchez, S.G. Moore, M. McDonald, R. Rodrigues, M.F. Morales, L. Orsi de Freitas, F. Randi, J. Furlong, J.A. Browne, M.B. Rabaglino, P. Lonergan, S.T. Butler

“…Following in vitro maturation and fertilization, presumptive zygotes were cultured in vitro to the blastocyst stage. …”
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Hepes na produção de embriões bovinos in vitro Hepes on in vitro production of bovine embryos by Marcelo Marcos Montagner, Paulo Bayard Dias Gonçalves, Jairo Pereira Neves, Luís Fabiano Santos da Costa, Ederson Bisognin Bortolotto, Alexandre Morales Farias, Patrícia Stranieri

“…A third experiment was designed to determine the effect of HEPES on embryo development. The zygotes were divided in two groups and co-incubated with oviduct epithelial cells in modified KSOM with 10% of fetal bovine serum without HEPES (n = 95) or with 25.0mM of HEPES (n = 92). …”
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