Use of peptide asparginyl ligases (PALs) in generating alternative non-Ig protein-drug conjugates for cancer therapy by See, Julia Jing Yi

“…Past research on antibody–drug conjugates (ADCs) focused on protein site-specific modification as chemical conjugation lacks site-selectivity. …”
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Solublizing the insoluble by Ang, Grace Pei Ting

“…Biochemical and structural research on target proteins has often been hindered by the difficulties faced in protein purification as proteins are known to aggregate in unsuitable environments. …”
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Co-translational integration of the light harvesting complex II and its associated pigments into polymeric membranes by Zapf, Thomas

“…Centrifugal microfiltration in means of polymersome purification as well as the development of a novel silica nanoparticles based purification method are further presented in this work. …”
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Rejection of pharmaceuticals by forward osmosis membranes by Jin, Xue, Shan, Junhong, Wang, Can, Wei, Jing, Tang, Chuyang Y.

“…These results can be important for FO membrane synthesis, modification and their application in water purification.…”
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A microfluidic platform to study pathogen-host interactions at single cell level by Zhang, Rui

“…Continuous flow of the organic phase increases the interfacial contact with the aqueous phase to achieve purification of target nucleic acid through phase partitioning. …”
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Synthesis and application of titanium dioxide nanotube and composites by Liang, Kun

“…The second application is on photocatalysis for water purification. Various TiO2 nanotube and TiO2-carbon composite with 3D structures (TiO2 nanotube– CNT, 3D Graphite – TiO2 coating and CNT – TiO2 coating) were fabricated and tested for their photocatalytic properties in water purification. …”
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Polyphenoloxidase from guava (Psidium guajava L.) by Augustin, M.A., Ghazali, Hasanah M., Hashim, Hasimah

“…Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. …”

Understanding electrostatic interaction on strong cation-exchanger via co-ion valency effects by Essert, GM, de Souza, JP, Schwaminger, SP, Bazant, MZ, Berensmeier, S

“…Altogether, our work may improve material designing and process development and, thereby, help to overcome the concurrent technological and economic bottlenecks of the well-deployed purification step of IEC.…”
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Structure and subunit arrangement of Mycobacterial F1FO ATP synthase and novel features of the unique mycobacterial subunit δ by Kamariah, Neelagandan, Huber, Roland G., Nartey, Wilson, Bhushan, Shashi, Bond, Peter J., Grüber, Gerhard

“…Expression and purification of the fused mycobacterial b-δ24-446 construct, excluding the membrane-embedded N-terminal amino acids, provided insight into its secondary structure. …”
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Optimised “green solvent” extraction of long-chain menaquinones (Vitamin K2) from wet Lactococcus lactis biomass by Lee, Sze Ying, Hu, Xiao, Stuckey, David C.

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Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach by Tam, Yew Joon

“…Following the release of HBsAg from cell disruption, univariate screening approaches on factors affecting the purification performance of HBsAg on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) techniques and the establishment of a two-step purification strategy were performed. …”
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Enhancing Protein Crystal Nucleation Using In Situ Templating on Bioconjugate-Functionalized Nanoparticles and Machine Learning by McCue, Caroline, Girard, Henri-Louis, Varanasi, Kripa K.

“…Although protein crystallization offers a promising alternative to chromatography for lower-cost protein purification, slow nucleation kinetics and high protein concentration requirements are major barriers for using crystallization as a viable strategy in downstream protein purification. …”
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Microfluidic lab-on-chip advances for liquid–liquid extraction process studies by Maurice, Ange, Theisen, Johannes, Gabriel, Jean-Christophe P.

“…The ever-increasing complexity of industrial product recycling calls for more efficient purification processes such as liquid–liquid (L/L) extraction. …”
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Novel Polymeric Materials for Environmental Applications / by Das, Paramita, editor 655455, Das, Subhasis (Subhasis K.), editor 655456, World Scientific (Online service) 655444

“…"Polymeric materials play an essential and ubiquitous role in many fields including structural and packaging materials, drug development, tissue engineering, wastewater treatment, pollutant removal, separation, water purification, smart agriculture, and even road and building construction. …”
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Microfiltration performance with two-phase flow by Qian, Guanglei, Zhou, Jiti, Zhang, Jinsong, Chen, Congcong, Jin, Ruofei, Liu, Wantao

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Production of single-chain variable fragment epidermal growth factor receptor (scFv-EGFR) antibody by Loh, Valerie Kai Li.

“…Affinity chromatography and size-exclusion chromatography via Fast Protein Liquid Chromatography (FPLC) are the methods of purification and protein products are characterised using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and/or Western blot. …”
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Vaccines: The Health of Nations - A Paradigm Shift by Datta, Shoumen

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Effect of air bubbling on atrazine adsorption in water by powdered activated carbons : competitive adsorption of impurities by Wang, Rong, Krantz, William B., Jia, Yue, Fane, Anthony Gordon

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Synthesis and characterization of mesoporous expanded graphite modified with PA/H3PO4 for enhanced oil sorption efficiency by Elbidi, Moammar, Mohd Salleh, Mohamad Amran, Gunam Resul, Mohamad Faiz Mukhtar, Rashid, Suraya Abdul

“…The global challenge of addressing oil spills in the ocean arises from their mobility and limited self-purification capacity, resulting in substantial and enduring harm to aquatic ecosystems and the depletion of valuable resources. …”

Use of Transient Transfection for cGMP Manufacturing of eOD-GT8 60mer, a Self-Assembling Nanoparticle Germline-Targeting HIV-1 Vaccine Candidate by Sharma, Vaneet K., Menis, Sergey, Brower, Evan T., Sayeed, Eddy, Ackland, Jim, Lombardo, Angela, Cottrell, Christopher A., Torres, Jonathan L., Hassell, Thomas, Ward, Andrew B., Tsvetnitsky, Vadim, Schief, William R.

“…Production was carried out via transient expression in the human embryonic kidney 293 (HEK293) cell line followed by a combination of purification techniques. A large-scale cGMP (200 L) production run yielded 354 mg of the purified eOD-GT8 60mer drug product material, which was formulated at 1 mg/mL in 10% sucrose in phosphate-buffered saline (PBS) at pH 7.2. …”
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