A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial cells in minute sample volume followed by direct on-chip quantitative PCR assay by Zhang, Rui, Gong Haiqing, Thomas, Zeng, Xu Dong, Lou, Chao-Ping, Sze, Chun Chau

“…Purification of nucleic acids from a low quantity of bacterial cells in minute volume is important in many clinical and biological applications. …”
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Sequence- and structure-specific probing of RNAs by short nucleobase-modified dsRNA-binding PNAs incorporating a fluorescent light-up uracil analog by Krishna, Manchugondanahalli Shivakumar, Toh, Desiree-Faye Kaixin, Meng, Zhenyu, Ong, Alan Ann Lerk, Wang, Zhenzhang, Lu, Yunpeng, Xia, Kelin, Prabakaran, Mookkan, Chen, Gang

“…We have successfully developed nucleobase-modified dsRNA-binding PNAs (dbPNAs) to facilitate structure-specific and selective recognition of dsRNA over single-stranded RNA (ssRNA) and dsDNA regions at near-physiological conditions. …”
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Exploring the induction of preproinsulin-specific Foxp3+ CD4+ Treg cells that inhibit CD8+ T cell-mediated autoimmune diabetes by DNA vaccination by Stifter, Katja, Schuster, Cornelia, Schlosser, Michael, Boehm, Bernhard Otto, Schirmbeck, Reinhold

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Incidence and mortality rates of 14 site-specific infectious diseases in 10 diverse areas of China: findings from China Kadoorie Biobank, 2006-2018 by Huang, R, Kartsonaki, C, Turnbull, I, Pei, P, Chen, Y, Liu, J, Du, H, Sun, D, Yang, L, Barnard, M, Lv, J, Yu, C, Chen, J, Li, L, Chen, Z, Bragg, F

“…The observed burden of, and disparities in, site-specific infections can inform targeted prevention efforts.…”

Relative deficiency in interferon-γ-secreting CD4+ T cells is strongly associated with poorer COVID-19 vaccination responses in older adults by Ho, Vanda W. T., Boon, Low Heng, Cui, Jianzhou, Juequn, Zhou, Shunmuganathan, Bhuvaneshwari, Gupta, Rashi, Tan, Nikki Y. J., Qian, Xinlei, Purushotorman, Kiren, Fong, Siew-Wai, Renia, Laurent, Ng, Lisa F. P., Angeli, Veronique, Chen, Jinmiao, Kennedy, Brian K., Ong, Catherine W. M., Macary, Paul A.

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The cellular characterization of SARS-CoV-2 spike protein in virus-infected cells using the receptor binding domain binding specific human monoclonal antibodies by Chan, Conrad En-Zuo, Ng, Ching-Ging, Lim, Angeline Pei-Chew, Seah, Shirley Lay-Kheng, Chye, De-Hoe, Wong, Steven Ka-Khuen, Lim, Jie-Hui, Lim, Vanessa Zi-Yun, Lai, Soak-Kuan, Wong, Pui-San, Leong, Kok-Mun, Liu, Yi-Chun, Sugrue, Richard J., Tan, Boon Huan

“…While all five antibodies bound conformational-specific epitopes within SARS-CoV-2 spike (S) protein, only PD5, PD7, and SC23 were able to bind to the receptor binding domain (RBD). …”
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Analyses of human vaccine-specific circulating and bone marrow-resident B cell populations reveal benefit of delayed vaccine booster dosing with blood-stage malaria antigens by Barrett, JR, Silk, SE, Mkindi, CG, Kwiatkowska, KM, Hou, MM, Lias, AM, Kalinga, WF, Mtaka, IM, McHugh, K, Bardelli, M, Davies, H, King, LDW, Edwards, NJ, Chauhan, VS, Mukherjee, P, Rwezaula, S, Chitnis, CE, Olotu, AI, Minassian, AM, Draper, SJ, Nielsen, CM

“…Notably, a higher frequency of vaccine-specific (putatively long-lived) plasma cells was detected in the bone marrow of these delayed boosting vaccinees by ELISPOT and correlated strongly with serum IgG. …”

Waning of specific antibodies against Delta and Omicron variants five months after a third dose of BNT162b2 SARS-CoV-2 vaccine in elderly individuals by Goh, Yun Shan, Rouers, Angeline, Fong, Siew-Wai, Zhuo, Nicole Ziyi, Hor, Pei Xiang, Loh, Chiew Yee, Huang, Yuling, Neo, Vanessa Kexin, Kam, Isaac Kai Jie, Wang, Bei, Ngoh, Eve Zi Xian, Salleh, Siti Nazihah Mohd, Lee, Raphael Tze Chuen, Pada, Surinder, Sun, Louisa Jin, Ong, Desmond Luan Seng, Somani, Jyoti, Lee, Eng Sing, Maurer-Stroh, Sebastian, Wang, Cheng-I., Leo, Yee Sin, Ren, Ee Chee, Lye, David C., Young, Barnaby Edward, Ng, Lisa F. P., Renia, Laurent

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Understanding lived experiences of bereaved parents of children with chronic life-threatening Illness : towards a culture-specific and meaning-oriented narrative E-writing intervention (NeW-I) for anticipatory grief support in Singapore by Dutta, Oindrila

“…This doctoral research is a first-of-its kind series of studies that comprehensively and holistically examined the lived experience of bereaved parents globally and in Singapore; building on this novel body of knowledge, it further developed and piloted a culture-specific and evidence-based intervention to support parent-caregivers of children with chronic life-threatening illness. …”
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Dialectic of digital enlightenment: reclaiming radical philosophy for our times by Nayak, Bhabani Shankar

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Global age-sex-specific mortality, life expectancy, and population estimates in 204 countries and territories and 811 subnational locations, 1950–2021, and the impact of the COVID-19 pandemic: a comprehensive demographic analysis for the Global Burden of Disease Study 2021 by Schumacher, AE, Kyu, HH, Aali, A, Almidani, O, Bandyopadhyay, S, Basnyat, B, Maude, RJ, Bennett, D, Browne, AJ, Dolecek, C, Dunachie, SJ, Newton, CRJ, Rodriguez, JAB

“…Life expectancy was calculated using age-specific mortality rates and standard demographic methods. …”

16.355J / ESD.355J Advanced Software Engineering, Fall 2002 by Leveson, Nancy

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Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering by Low, Pearlie Si Jia, Tjin, Monica Suryana, Fong, Eileen

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21W.780 Communicating in Technical Organizations, Spring 2006 by Barrett, Edward C., Bentley, Frank

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16.01-04 Unified Engineering I, II, III, & IV, Fall 2003-Spring 2004 by Hall, Steven Ray, Coleman, Charles P, Drela, Mark, Lundqvist, Ingrid Kristina, Spearing, S Mark, Waitz, Ian A, Young, Peter W

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Feasibility of Vector Instruction-Set Semantics Using Abstract Monads by De Belen, Arthur Reiner

“…I answer this question with a tentative “yes”, backed by a partial specification in Haskell of a small but nontrivial subset of this vector extension, a translation of the same specification into Coq using hs-to-coq², and work towards demonstrating the utility of this specification.…”
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Solublizing the insoluble by Ang, Grace Pei Ting

“…Biochemical and structural research on target proteins has often been hindered by the difficulties faced in protein purification as proteins are known to aggregate in unsuitable environments. …”
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Co-translational integration of the light harvesting complex II and its associated pigments into polymeric membranes by Zapf, Thomas

“…Centrifugal microfiltration in means of polymersome purification as well as the development of a novel silica nanoparticles based purification method are further presented in this work. …”
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A microfluidic platform to study pathogen-host interactions at single cell level by Zhang, Rui

“…On-chip quantitative PCR was carried out with species-specific primer and probe sets to quantify the adhered bacteria. …”
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Design and construction of a low cost compliant hand robot by Yu, Wei Shin., Choo, Fook Hoong.

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